Qualitative and quantitative analyses of influenza virus receptors in trachea and lung tissues of humans,mice,chickens and ducks

作     者：WANG BaoLin LIU Bo CHEN Ling ZHANG JianYong HE HongXuan ZHANG Hong 

作者机构：Laboratory of Respiratory Medicine Department of Respiratory Medicine Affiliated Hospital of Zunyi Medical College Zunyi 563003 China National Research Centerfor Wildlife-borne Diseases Institute of Zoology Chinese Academy af Sciences Beijing 100101 China Z-BioMed Inc. Rockville MD 20855 USA 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2012年第55卷第7期

页      面：612-617页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 100705[医学-微生物与生化药学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 071005[理学-微生物学] 10[医学] 

基　　金：supported by the Governor’s Fund of Guizhou Province for Outstanding Individuals in Science Technology and Education(Grant No. 2010-68) 

主　　题：influenza virus siaUc acid receptors trachea lung lectin immunofluorescence quantitative analysis qualitative analysis 

摘      要：To accurately determine the expression and distribution patterns of two infuenza virus receptors （SAa2,3-gal and SAa2,6-gal） in trachea and lung tissues of humans, mice, chickens and ducks, we analyzed lectin immunofluorescence stainings of various tissue sections qualitatively and quantitatively. Results from the qualitative analysis showed that both influenza virus receptors were expressed in lung tissues of humans, mice, chickens and ducks as well as trachea tissues of mice and ducks. However, SAa2,6-gal receptor was expressed only in the human trachea tissue and SAa2,3-gal receptor was expressed only in the chicken trachea tissue. Results from the quantitative analysis demonstrated that both receptors were expressed in trachea tis- sues of human and mouse, as well as in lung tissues of humans, chickens and ducks. Meanwhile, our results also showed that the expression and distribution of influenza virus receptors in the same tissue were not always uniform, indicating that their distribution and expression in various tissues are not simply the distinction between the presence or absence of receptors, but rather the difference in the amount of expressed receptors.