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TGF-β2 downregulates osteogenesis under inflammatory conditions in dental follicle stem cells

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International Journal of Oral Science 2018年第4期10卷 232-243页

作者： Soyoun Um Joo-Hee Lee Byoung-Moo Seo Biotooth Engineering Lab Dental Research InstituteDental Regenerative BiotechnologyDepartment of Dental ScienceSchool of DentistrySeoul National University Biotooth Engineering Lab Department of Oral and Maxillofacial Surgery and Craniomaxillofacial Life ScienceDental Research InstituteSchool of DentistrySeoul ational University

Bone formation is important for the reconstruction of bone-related structures in areas that have been damaged by *** conditions such as those that occur in patients with rheumatoid arthritis, cystic fibrosis, and periodontitis have been shown to inhibit osteoblastic differentiation. This study focussed on dental follicle stem cells(DFSCs), which are found in developing tooth germ and participate in the reconstruction of alveolar bone and periodontal tissue in periodontal disease. After bacterial infection of inflamed dental tissue, the destruction of bone was observed. Currently, little is known about the relationship between the inflammatory environment and bone formation. Osteogenic differentiation of inflamed DFSCs resulted in decreased alkaline phosphatase(ALP) activity and alizarin red S staining compared to normal DFSCs. Additionally, in vivo transplantation of inflamed and normal DFSCs demonstrated severe impairment of osteogenesis by inflamed DFSCs. Protein profile analysis via liquid chromatography coupled with tandem mass spectrometry was performed to analyse the differences in protein expression in inflamed and normal tissue. Comparison of inflamed and normal DFSCs showed significant changes in the level of expression of transforming growth factor(TGF)-β2. Porphyromonas gingivalis(P.g.)-derived lipopolysaccharide(LPS) was used to create in vitro inflammatory conditions similar to periodontitis. The osteogenic differentiation of LPS-treated DFSCs was suppressed, and the cells displayed low levels of TGF-β1 and high levels of TGF-β2. DFSCs treated with TGF-β2 inhibitors showed significant increases in alizarin red S staining and ALP activity. TGF-β1 expression was also increased after inhibition of TGF-β2. By examining inflamed DFSCs and LPS-triggered DFSCs, these studies showed both clinically and experimentally that the increase in TGF-β2 levels that occurs under inflammatory conditions inhibits bone formation.

关键词： TGF downregulates osteogenesis under inflammatory conditions in dental follicle stem cells

Systematic review of anastomotic complications of esophagojejunostomy after laparoscopic total gastrectomy

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World Journal of Gastroenterology 2015年第32期21卷 9656-9665页

作者： Mikito Inokuchi Sho Otsuki Yoshitaka Fujimori Yuya Sato Masatoshi Nakagawa Kazuyuki Kojima Department of Surgical Oncology Tokyo Medical and Dental University Department of Minimally Invasive Surgery Tokyo Medical and Dental University

AIM: To investigate the anastomotic complications ofesophagojejunostomy(EJS) after laparoscopic total gastrectomy(LTG), we reviewed retrospective ***: A literature search was conducted in Pub Med for studies published from January 1, 1994 through January 31, 2015. The search terms included "laparoscopic," "total gastrectomy," and "gastric cancer." First, we selected 16 non-randomized controlled trials(RCTs) comparing LTG with open total gastrectomy(OTG) and conducted an updated meta-analysis of anastomotic complications after total gastrectomy. The Newcastle-Ottawa scoring system(NOS) was used to assess the quality of the non-RCTs included in this study. Next, we reviewed anastomotic complications in 46 case studies of LTG to compare the various procedures for EJS. RESULTS: The overall incidence of anastomotic leakage associated with EJS was 3.0%(30 of 984 patients) among LTG procedures and 2.1%(31 of 1500 patients) among OTG procedures in the 16 non-RCTs. The incidence of anastomotic leakage did not differ significantly between LTG and OTG(odds OR = 1.42, 95%CI: 0.86-2.33, P = 0.17, I2 = 0%). Anastomotic stenosis related to EJS was reported in 72(2.9%) of 2484 patients, and the incidence was 3.2% among LTG procedures and 2.7% among OTG procedures. The incidence of anastomotic stenosis related to EJS was slightly, but not significantly, higher in LTG than in OTG(OR = 1.55, 95%CI: 0.94-2.54, P = 0.08, I2 = 0%). The various procedures for LTG were classified into six categories in the review of case studies of LTG. The incidence of EJS leakage was similar(1.1% to 3.2%), although the incidence of EJS stenosis was relatively high when the Or VilTM device was used(8.8%) compared with other procedures(1.0% to 3.6%).CONCLUSION:The incidence of anastomotic complications associated with EJS was not different between LTG and OTG. Anastomotic stenosis was relatively common when the Or VilTM device was used.

关键词： Gastric cancer Laparoscopic Gastrectomy Anastomosi

Extrapolating neurogenesis of mesenchymal stem/stromal cells on electroactive and electroconductive scaffolds to dental and oral-derived stem cells

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International Journal of Oral Science 2022年第2期14卷 123-131页

作者： Boon Chin Heng Yunyang Bai Xiaochan Li Xuehui Zhang Xuliang Deng Central Laboratory Peking University School and Hospital of StomatologyBeijingChina School of medical and Life Sciences Sunway UniversitySubang JayaSelangor Darul EhsanMalaysia Department of Geriatric Dentistry Peking University School and Hospital of StomatologyBeijingChina Department of dental Materials&dental medical Devices Testing center Peking University School and Hospital of StomatologyBeijingChina National Engineering Laboratory for Digital and Material Technology of Stomatology NMPA Key Laboratory for Dental MaterialsBeijing Laboratory of Biomedical Materials&Beijing Key Laboratory of Digital StomatologyPeking University School and Hospital of StomatologyBeijingChina

The high neurogenic potential of dental and oral-derived stem cells due to their embryonic neural crest origin,coupled with their ready accessibility and easy isolation from clinical waste,make these ideal cell sources for neuroregeneration ***,these cells also have high propensity to differentiate into the osteo-odontogenic *** strategy to enhance neurogenesis of these cells may be to recapitulate the natural physiological electrical microenvironment of neural tissues via electroactive or electroconductive tissue engineering ***,to date,there had been hardly any such studies on these *** relevant scientific information comes from neurogenesis of other mesenchymal stem/stromal cell lineages(particularly bone marrow and adipose tissue)cultured on electroactive and electroconductive scaffolds,which will therefore be the focus of this *** there are larger number of similar studies on neural cell lines(***12),neural stem/progenitor cells,and pluripotent stem cells,the scientific data from such studies are much less relevant and less translatable to dental and oral-derived stem cells,which are of the mesenchymal *** extrapolation work is needed to validate that electroactive and electroconductive scaffolds can indeed promote neurogenesis of dental and oral-derived stem cells,which would thus facilitate clinical applications in neuroregeneration therapy.

关键词： mesenchymal conductive neural

Magnesium/Nano-hydroxyapatite Composite for Bone Reconstruction: The Effect of Processing Method

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Journal of Bionic Engineering 2020年第1期17卷 92-99页

作者： Mohammad Khodaei Farahnaz Nejatidanesh Mohammad Javad Shirani Srinivasan Iyengar Hossein Sina Omid Savabi Department of Materials Science and Engineering Golpayegan University of TechnologyGolpayegan ***Iran dental Materials Research center Dental Research InstituteSchool of DentistryIsfahan University of Medical SciencesIsfahan ***Iran Post graduate student of Prosthodontics Student Research CommitteeSchool of DentistryIsfahan University of Medical SciencesIsfahan ***Iran Materials Engineering Division Faculty of EngineeringLund UniversityLund 11822100Sweden European Spallation Source ERIC Lund 17622100Sweden dental Research center Dental Research InstituteSchool of DentistryIsfahan University of Medical SciencesIsfahan ***Iran

Nano-ceramic particles can serve as reinforcing agents for metallic materials to improve their mechanical ***,it is important to ensure chemical compatibility between the matrix and *** the present study,magnesium composites with and without nano-hydroxyapatite(nHA)particles were fabricated for bone reconstruction *** different techniques were used,Conventional Sintering(CS)of powder compacts and Spark Plasma Sintering(SPS)of pre-compacted *** showed that a 10 wt%addition of nHA particles to magnesium,followed by SPS improved the compression strength by 27%.CS did not lead to any significant improvement compared to SPS processing.X-ray diffraction data after CS revealed the formation of unfavorable phases due to chemical reactions between nHA particles and the magnesium matrix,while these phases were absent after SPS *** mechanical properties of the specimens fabricated by CS were much inferior to those processed using *** shorter processing time associated with SPS leaded to reduced interaction between nHA particles and the Mg-matrix,compared to CS.

关键词： magnesium nano-hydroxyapatite composite materials in situ reaction biomedical-materials

Clinical significance of MET in gastric cancer

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World Journal of Gastrointestinal Oncology 2015年第11期7卷 317-327页

作者： Mikito Inokuchi Sho Otsuki Yoshitaka Fujimori Yuya Sato Masatoshi Nakagawa Kazuyuki Kojima Department of Gastrointestinal Surgery Tokyo Medical and Dental University Department of Minimally Invasive Surgery Tokyo Medical and Dental University

Chemotherapy has become the global standard treatment for patients with metastatic or unresectable gastric cancer(GC),although outcomes remain *** molecular-targeted therapies inhibiting signaling pathways of various tyrosine kinase receptors have been developed,and monoclonal antibodies targeting human epidermal growth factor receptor 2 or vascular endothelial growth factor receptor 2 have become standard therapy for *** growth factor and its receptor,c-MET(MET),play key roles in tumor growth through activated signaling pathways from receptor in GC *** amplification of MET leads to the aberrant activation found in GC tumors and is related to survival in patients with *** review discusses the clinical significance of MET in GC and examines MET as a potential therapeutic target in patients with *** studies in animal models have shown that MET antibodies or smallmolecule MET inhibitors suppress tumor-cell proliferation and tumor progression in MET-amplified GC *** drugs are now being evaluated in clinical trials as treatments for metastatic or unresectable GC.

关键词： MET Gastric cancer Genomic amplification Immunohis

Management of gastric and duodenal neuroendocrine tumors

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World Journal of Gastroenterology 2016年第30期22卷 6817-6828页

作者： Yuichi Sato Satoru Hashimoto Ken-ichi Mizuno Manabu Takeuchi Shuji Terai Department of Gastroenterology Niigata University Graduate School of Medical and Dental Sciences Division of Endoscopy Niigata University Medical and Dental Hospital

Gastrointestinal neuroendocrine tumors (GI-NETs) are rare neoplasms, like all NETs. However, the incidence of GI-NETS has been increasing in recent years. Gastric NETs (G-NETs) and duodenal NETs (D-NETs) are the common types of upper GI-NETs based on tumor location. G-NETs are classified into three distinct subgroups: type I, II, and III. Type I G-NETs, which are the most common subtype (70%-80% of all G-NETs), are associated with chronic atrophic gastritis, including autoimmune gastritis and Helicobacter pylori associated atrophic gastritis. Type II G-NETs (5%-6%) are associated with multiple endocrine neoplasia type 1 and Zollinger-Ellison syndrome (MEN1-ZES). Both type I and II G-NETs are related to hypergastrinemia, are small in size, occur in multiple numbers, and are generally benign. In contrast, type III G-NETs (10%-15%) are not associated with hypergastrinemia, are large-sized single tumors, and are usually malignant. Therefore, surgical resection and chemotherapy are generally necessary for type III G-NETs, while endoscopic resection and follow-up, which are acceptable for the treatment of most type I and II G-NETs, are only acceptable for small and well differentiated type III G-NETs. D-NETs include gastrinomas (50%-60%), somatostatin-producing tumors (15%), nonfunctional serotonin-containing tumors (20%), poorly differentiated neuroendocrine carcinomas (< 3%), and gangliocytic paragangliomas (< 2%). Most D-NETs are located in the first or second part of the duodenum, with 20% occurring in the periampullary region. Therapy for D-NETs is based on tumor size, location, histological grade, stage, and tumor type. While endoscopic resection may be considered for small nonfunctional D-NETs (G1) located in the higher papilla region, surgical resection is necessary for most other D-NETs. However, there is no consensus regarding the ideal treatment of D-NETs.

关键词： Gastric neuroendocrine tumors Duodenal neuroendocrine tumors Classification Endoscopic treatment Endoscopic submucosal dissection

Effect of grain boundary segregation on aging resistance and mechanical properties of tetravalent element-doped 3Y-TZP ceramics for dental restoration

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International Journal of Smart and Nano Materials 2023年第2期14卷 170-188页

作者： Bin Deng Rongfang Zou Yang Huang Lu Feng Yanru Chen Department of Stomatology the First Medical CenterChinese PLA General HospitalBeijingChina Chinese PLA medical School BeijingChina

In the humid oral environment,3Y-TZP ceramics always suffer from low-temperature degradation(LTD)for a long time,which results in the degradation of mechanical properties and catastrophic *** low-temperature degradation(LTD)and mechanical properties of low-cost tetravalent(Ge^(4+),Ti^(4+))element-doped 3Y-TZP were investigated by analysing grain boundary segregation in samples with deferent *** results show that GeO_(2) is superior to TiO_(2) in limiting LTD but results in lower flexural strength and fracture toughness when the content is≥1.5 mol%.This dilemma can be improved by adding only 0.1%-0.5 wt%Al_(2)O_(3),and the flexural strength and fracture toughness of 0.25 wt% Al_(2)O_(3) zirconia are then increased to 898 MPa and 4.68 MPa·m^(1/2) compared with 1Ge-3Y,*** work is expected to provide an effective reference for the development and application of budget dental materials.

关键词： 3Y-TZP low-temperature degradation grain boundary segregation mechanical properties GeO_(2)

Fabrication of Porous Mg-Zn Scaffold through Modified Replica Method for Bone Tissue Engineering

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Journal of Bionic Engineering 2018年第5期15卷 907-913页

作者： Amir Hamed Aghajanian Bijan Abbasi Khazaei Mohammad Khodaei Mohammad Rafienia Department of Materials Engineering Faculty of Engineering Razi University Kermanshah Iran dental Research center School of Dentistry Isfahan University of Medical Sciences Isfahan 81746-73461 Iran Biosensor Research center lsfahan University of Medical Sciences Isfahan 81744176 Iran

Biodegradable scaffolds are essential parts in hard tissue engineering. A highly porous magnesium-zinc （Mg-Zn 4 wt.%） scaffold with different Mg-Zn powder to liquid media ratios （50 wt.%, 70 wt.% and 90 wt.%） and different concentrations of ethanol （0 vol.%, 10 vol.%, 20 vol.% and 40 vol.%） were prepared through modified replica method. The mechanical properties were assessed through compression test and the structures of scaffolds were examined by Scanning Electron Microscope （SEM）. Results show that, the increase in Mg-Zn powder to liquid media ratio （50 wt.% to 90 wt.%） in ethanol free slurry, increases the thickness of struts （37 lam to 74 lam） and the plateau stress （0.5 MPa to 1.4 MPa）. The results obtained from X-ray Diffractometry （XRD） and compression test indicate that consuming ethanol in liquid media of replica, results in higher plateau stress by 46% due to less Mg-water reaction and no formation of Mg（OH）2 in the scaffold. The results of porosity measurement indicate that water-ethanol mixture composition and different solid fractions have no significant effects on true and apparent porosities of the fabricated scaffolds.

关键词： porous magnesium scaffold replica method Mg-Zn powder to water ratio mechanical properties

A case of diffuse alveolar hemorrhage following synthetic cathinone inhalation

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World Journal of Emergency Medicine 2020年第3期11卷 182-184页

作者： Masakazu Nitta Taro Tamakawa Natsuo Kamimura Tadayuki Honda Hiroshi Endoh Advanced Emergency and Critical Care center Niigata University Medical and Dental HospitalChuo-kuNiigataJapan

Dear editor,Diffuse alveolar hemorrhage(DAH)sometimes causes a life-threatening condition;thus,prompt diagnosis and treatment for DAH is ***,a variety of diseases(e.g.,systemic autoimmune diseases,infectious diseases,drugs)are associated with the development of DAH,which occasionally causes diffi culty with identifying the specifi c etiology.

关键词： alveolar diseases diagnosis