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PRKCDBP Methylation is a Potential and Promising Candidate Biomarker for Non-small Cell Lung Cancer

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中国肺癌杂志 2022年第2期25卷 78-85页

作者： Jing LI Lin QI Mingfang ZHANG Caiyun YAO Jinan FENG Zhonghua ZHENG Chujia CHEN Shiwei DUAN Yuanlin QI school of Basic medical Sciences Fujian Medical UniversityFuzhou 350122China medical Genetics Center School of MedicineNingbo UniversityNingbo 315211China

Background and objectives:The occurrence and development of lung cancer are closely linked to epigenetic modification.Abnormal DNA methylation in the CpG island region of genes has been found in many cancers.Protein kinase C delta binding protein(PRKCDBP) is a potential tumor suppressor and its epigenetic changes are found in many human malignancies.This study investigated the possibility of PRKCDBP methylation as a potential biomarker for non-small cell lung cancer(NSCLC).Methods:We measured the methylation levels of PRKCDBP in the three groups of NSCLC tissues.Promoter activity was measured by the dual luciferase assay,with S’-aza-deoxycytidine to examine the effect of demethylation on the expression level of PRKCDBP.Results:The methylation levels of PRKCDBP in tumor tissues and 3 cm para-tumor were higher than those of distant(>10 cm)non-tumor tissues.Receiver operating characteristic(ROC) curve analysis between tumor tissues and distant non-tumor tissues showed that the area under the line(AUC) was 0.717.Dual luciferase experiment confirmed that the promoter region was able to promote gene expression.Meanwhile,in vitro methylation of the fragment(PRKCDBP;e) could significantly reduce the promoter activity of the fragment.Demethylation of 5’-aza-deoxycytidine in lung cancer cell lines A549 and H1299 showed a significant up-regulation of PRKCDBP mRNA levels.Conclusion:PRKCDBP methylation is a potential and promising candidate biomarker for non-small cell lung cancer.

关键词： Lung neoplasms PRKCDBP DNA methylation

Reducing Labor Anxiety with Auriculotherapy:A Randomized Clinical Trial Study

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Chinese Journal of Integrative Medicine 2022年第5期28卷 440-444页

作者： Katayon Vakilian Mehrnoush Ghaemmaghami Narges Sheikhganbari Fatemeh Shabani Masoomeh Vahedi Department of Midwifery School of MedicineTraditional and Complementary Medicine Research CenterArak University of Medical SciencesArak(***)Iran Medical School Arak University of Medical SciencesArak(***)Iran Nursing-Midwifery school Iran University of Medical SciencesTehran(***)Iran

Objectives:To investigate the effect of auriculotherapy on reducing labor anxiety.Methods:In this randomized clinical trial,166 nulliparous pregnant women admitted to the Labor Ward of Izadi Hospital in Qom,Iran were included and assigned to two groups by randomized block design.In the intervention group(83 cases),auriculotherapy in"Shenmen,Thalamic,Zero,Brain,and Brain stem"was performed in dilatations of 4,6 and 8 cm,between the contractions for 30 s in both ears at interval 1 h.While the control group received routine care.The Spielberger Inventory were completed pre-and post-interventions.Results:The auriculotherapy was effective to reduce anxiety state in the intervention group compared to the control group(P=0.001).The score of state anxiety in the intervention group reduced for 7.0,8.0 and 11.0 U in 4,6,and 8 cm dilatations after the intervention.The score of trait anxiety in the intervention group reduced for 3.8,4.0 and 8.3 U in 4,6,and 8 cm dilatations after the intervention.There was no significant difference of delivery mode and newborns’weight and Apgar score between groups(P>0.05).No adverse postpartum outcome such as hemorrhage has been observed in both groups.Conclusion:Since most women are anxious during labor,it seems that auriculotherapy can be an easy and safe method to reduce labor anxiety.(Trial registration No.irct20121230011944 n4)

关键词： auriculotherapy childbirth pain anxiety

Cross-Sectional Online Survey on Depression and Anxiety Among the Population Infected or Non-Infected with COVID-19——China,December 2022

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China CDC weekly 2023年第11期5卷 235-240页

作者： Tingting Qin Xingming Li Mingyu Gu Yao Wang Yutong Yang Xinyuan Bai Kun Qiao school of Public Health Capital Medical UniversityBeijingChina

Summary What is already known about this topic?The psychological impact of the large-scale infection of the population resulting from the end of lockdown measures in China during the coronavirus disease 2019(COVID-19)pandemic is unknown.What is added by this report?Among all participants,55.7%had depression symptoms,with a significant difference between the infected and non-infected groups,and 30.1%had anxiety.Those who were young,unvaccinated,had lower incomes,and experienced chronic diseases were more likely to experience negative emotions.What are the implications for public health practice?Government officials should take into account the effect of policies on public sentiment during similar public health events and implement tailored community interventions to address any negative sentiment.

关键词： Infect likely Population

Royal-jelly-based apitherapy can attenuate damages to male reproductive parameter following nicotine administration

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Animal Models and Experimental Medicine 2022年第2期5卷 133-140页

作者： Mahsa Nazar-Zadeh Cyrus Jalili Amene Nikgoftar Fathi Ali Ghanbari Mitra Bakhtiari Department of Anatomical Sciences Medical SchoolKermanshah University of Medical SciencesKermanshahIran medical Biology Research Center Kermanshah University of Medical SciencesKermanshahIran Department of Anatomical Sciences Medical SchoolArak University of Medical SciencesArakIran

Background:Nicotine administration can generate severe oxidative stress and lipid peroxidation.Royal jelly,with its antioxidant properties,acts as a scavenger of reactive oxygen species.This study describes the apitherapy effects of royal jelly on testicular damage following nicotine administration.Methods:Forty-eight male BALB/c mice were divided into 8 groups(n=6):saline,3 different doses of royal jelly(100,150,and 200 mg/kg body weight(BW)per day),nicotine(1.5 mg/kg),and 3 different groups of Nic+Roy(1.5 mg/kg of Nic+100,150,and 200 mg/kg BW per day of royal jelly).Nicotine was administrated intraperitoneally,and royal jelly was prescribed orally for 10 consecutive days.Serum levels of hormones(testosterone,luteinizing hormone,and follicle-stimulating hormone),total antioxidant capacity,nitric oxide(NO)status,malondialdehyde levels,sperm DNA fragmentation,sperm parameters,histopathological changes(H&E staining),immunohistochemistry against apoptotic proteins,and gene expression of Bcl-2,p53,Caspase-3,and Nrf2(real-time PCR)were assessed to evaluate the molecular and histological changes.Results:Hormone levels,sperm parameters,and status of antioxidants were decreased significantly(p<.05)following nicotine administration.Moreover,royal jelly treatment normalized hormonal and antioxidant characteristics,decreased apoptotic gene expression,increased Nfr2 gene expression,and restored histopathological alteration to the physiological status significantly(p<.05).Conclusion:Royal jelly upregulates the antioxidant status,inhibits the mitochondrialdependent apoptosis pathway,and increases the rate of proliferation.This therapeutic agent effectively protected the testis against nicotine-associated damages by antioxidant and anti-apoptotic effects.

关键词： antioxidant apitherapy apoptosis damage male nicotine reproduction royal jelly testis

Single intratesticular injection of blood-serum-derived exosomes can potentially alleviate testopathy following testicular torsion

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Animal Models and Experimental Medicine 2022年第4期5卷 362-368页

作者： Mona Keivan Fatemeh Mansouri Torghabeh Samira Davoodi Shima Moradi Maryamneghari Reza Dadfar Member of Research Committee Medical SchoolKermanshah University of Medical SciencesKermanshahIran Department of Physiology Sciences Medical SchoolMashhad University of Medical SciencesMashhadIran Department of Anatomical Sciences Medical SchoolKermanshah University of Medical SciencesKermanshahIran Department of Anatomical Sciences Faculty of MedicineAJA University of Medical SciencesTehranIran

Background:Testicular torsion(TT)is an acute inflammatory process leading to male infertility.Today,anti-i nflammatory effects of exosomes derived from blood serum are used in various laboratory procedures.In the present study,the anti-i nflammatory effects of blood-serum-derived exosomes in treatment of acute inflammation following TT in mice were evaluated.Materials and Methods:Eighteen male mice were grouped as healthy control,TT,and TT+exosome.TT was induced surgically,and exosomes were extracted from blood serum and administrated by a single intratesticular injection(10 IU).Malondialdehyde(MDA)and Griess assays were used to evaluate the level of oxidative stress.Sperm indices,testosterone(Tes),and apoptotic gene expression(p-53,Bcl2,and Caspase-3)were also assessed.H&E and immunohistochemistry(IHC)stainings were used for histopathological investigations.Data analysis was applied by SPSS(v.19)software.Results:Oxidative stress and apoptotic genes expression were increased significantly(p<0.05)in TT group compared with control.Sperm parameters and Tes were significantly increased,and expression of apoptotic genes was significantly reduced in TT+exosome group(p<0.05).Conclusion:Since the blood-serum-derived exosomes have anti-i nflammatory features,the intratesticular application of blood-serum-derived exosomes can be used clinically in acute phase of orchitis following TT to inhibit testicular inflammation.

关键词： anti-i nflammation exosome parameter sperm testicular torsion

Updates on CRISPR-based gene editing in HIV-1/AIDS therapy

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Virologica Sinica 2022年第1期37卷 1-10页

作者： Zhihao Zhang Wei Hou Shuliang Chen Institute of medical Virology School of Basic Medical SciencesWuhan UniversityWuhan430071China

Although tremendous efforts have been made to prevent and treat HIV-1 infection,HIV-1/AIDS remains a major threat to global human health.The combination antiretroviral therapy(cART),although able to suppress HIV-1 replication,cannot eliminate the proviral DNA integrated into the human genome and thus requires lifelong treatment that may lead to various side effects.In recent years,clustered regularly interspaced short palindromic repeat(CRISPR)-associated nuclease 9(Cas9)related gene-editing systems have been developed and designed as effective ways to treat HIV-1 infection.However,new gene-targeting tools derived from or functioning like CRISPR/Cas9,including base editor,prime editing,SHERLOCK,DETECTR,PAC-MAN,ABACAS,pfAGO,have been developed and optimized for pathogens detection and diseases correction.Here,we summarize recent studies on HIV-1/AIDS gene therapy and provide more gene-editing targets based on studies relating to the molecular mechanism of HIV-1 infection.We also identify the strategies and potential applications of these new gene-editing technologies for HIV-1/AIDS treatment in the future.Moreover,we discuss the caveats and problems that should be addressed before the clinical use of these versatile CRISPR-based gene targeting tools.Finally,we offer alternative solutions to improve the practice of gene targeting in HIV-1/AIDS gene therapy.

关键词： HIV-1/AIDS CRISPR/Cas9 Gene editing Gene therapy

Mechanisms and applications of antitumor immunotherapy of responsive drug-loaded nanoparticles in breast cancer

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BIOCELL 2023年第7期47卷 1483-1498页

作者： LETIAN JIN HETING CHEN QI RUAN RUI LIU YIFENG FAN XIUFANG XU DAJIANG WANG JIAHUI LU school of medical Imaging Hangzhou Medical CollegeHangzhou310053China

During the chemotherapy of tumors,the cytotoxic effect of drugs is vital to kill tumor cells,and the delivery of a chemotherapeutic agent is of great importance for optimal therapeutic effects.The high in vivo clearance rate and low delivery efficiency of conventional chemotherapeutic agents affect the therapeutic effect.In recent years,the responsive drug delivery nanosystem has received increasing concern owing to its excellent biocompatibility,stable delivery performance,and controlled drug release strategies.To lucidly explain the cytocidal and immunotherapeutic effects of such responsive nanosystems in breast cancer,this review discusses the various stimuli and responses of drug-loaded liposomal nanosystems.The light/magnetic response of drug-loaded bionic membranes nanosystems and the heat/magnetic response of drug-loaded iron oxide nanosystems are also elaborated.Their cancer cell-killing efficacy and antitumor immunotherapeutic effects are also scrutinized.

关键词： Responsive nanoparticles Antitumor immunotherapy Breast cancer

Bystanders or not?Microglia and lymphocytes in aging and stroke

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Neural Regeneration Research 2023年第7期18卷 1397-1403页

作者： Justin N.Nguyen Anjali Chauhan University of Texas McGovern Medical School at Houston HoustonTXUSA Department of Neurology University of Texas McGovern Medical School at HoustonHoustonTXUSA

As the average age of the world population increases,more people will face debilitating aging-associated conditions,including dementia and stroke.Not only does the incidence of these conditions increase with age,but the recovery afterward is often worse in older patients.Researchers and health professionals must unveil and understand the factors behind age-associated diseases to develop a therapy for older patients.Aging causes profound changes in the immune system including the activation of microglia in the brain.Activated microglia promote T lymphocyte transmigration leading to an increase in neuroinflammation,white matter damage,and cognitive impairment in both older humans and rodents.The presence of T and B lymphocytes is observed in the aged brain and correlates with worse stroke outcomes.Preclinical strategies in stroke target either microglia or the lymphocytes or the communications between them to promote functional recovery in aged subjects.In this review,we examine the role of the microglia and T and B lymphocytes in aging and how they contribute to cognitive impairment.Additionally,we provide an important update on the contribution of these cells and their interactions in preclinical aged stroke.

关键词： age B lymphocytes brain central nervous system cognition inflammation microglia middle cerebral artery occlusion neuroinflammation stroke T lymphocytes white matter injury

A novel anticancer property of Lycium barbarum polysaccharide in triggering ferroptosis of breast cancer cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2022年第4期23卷 286-299页

作者： Xing DU Jingjing ZHANG Ling LIU Bo XU Hang HAN Wenjie DAI Xiuying PEI Xufeng FU Shaozhang HOU Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education School of Basic Medical SciencesNingxia Medical UniversityYinchuan 750004China Department of Clinical Pathology School of Basic Medical SciencesNingxia Medical UniversityYinchuan 750004China

Breast cancer is one of the most malignant tumors and is associated with high mortality rates among women.Lycium barbarum polysaccharide(LBP)is an extract from the fruits of the traditional Chinese herb,L.barbarum.LBP is a promising anticancer drug,due to its high activity and low toxicity.Although it has anticancer properties,its mechanisms of action have not been fully established.Ferroptosis,which is a novel anticancer strategy,is a cell death mechanism that relies on iron-dependent lipid reactive oxygen species(ROS)accumulation.In this study,human breast cancer cells(Michigan Cancer Foundation-7(MCF-7)and MD Anderson-Metastatic Breast-231(MDA-MB-231))were treated with LBP.LBP inhibited their viability and proliferation in association with high levels of ferroptosis.Therefore,we aimed to ascertain whether LBP reduced cell viability through ferroptosis.We found that the structure and function of mitochondria,lipid peroxidation,and expression of solute carrier family 7 member 11(SLC7 A11,also known as x CT,the light-chain subunit of cystine/glutamate antiporter system X_(c)^(-))and glutathione peroxidase 4(GPX4)were altered by LBP.Moreover,the ferroptosis inhibitor,Ferrostatin-1(Fer-1),rescued LBP-induced ferroptosis-associated events including reduced cell viability and glutathione(GSH)production,accumulation of intracellular free divalent iron ions and malondialdehyde(MDA),and down-regulation of the expression of x CT and GPX4.Erastin(x CT inhibitor)and RSL3(GPX4 inhibitor)inhibited the expression of x CT and GPX4,respectively,which was lower after the co-treatment of LBP with Erastin and RSL3.These results suggest that LBP effectively prevents breast cancer cell proliferation and promotes ferroptosis via the x CT/GPX4 pathway.Therefore,LBP exhibits novel anticancer properties by triggering ferroptosis,and may be a potential therapeutic option for breast cancer.

关键词： Lycium barbarum polysaccharide Ferroptosis Breast cancer cells xCT Glutathione peroxidase 4(GPX4)