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Study of Oxidation of Carbon Monoxide on the Surface of Sn-doped Carbon Nanotube

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Chinese Journal of Structural Chemistry 2019年第4期38卷 524-532页

作者： MEYSAM Najafi Medical Biology Research Center Kermanshah University of Medical Sciences

In recent years, the discovery of suitable catalyst to the oxidation of carbon monoxide(CO) is a major concern in industry. In present study, in the first step the carbon nanotube(CNT) with Sn was doped and the surface of Sn-CNT via O_2 molecule was activated. In the second step the CO oxidation on the surface of Sn-CNT via Langmuir Hinshelwood(LH) and Eley Rideal(ER) mechanisms was investigated. Results show that O_2-Sn-CNT can oxidize the CO molecule via Sn-CNT-O-O* + CO → Sn-CNT-O-O*-CO → Sn-CNT-O* + CO_2 and Sn-CNT-O* + CO → Sn-CNT + CO_2 reactions. Results show that CO oxidation on activated Sn-CNT surface via the ER mechanism has lower energy barrier than LH mechanism. Finally, calculated parameters reveal that activated Sn-CNT is an acceptable catalyst with high potential to the oxidation of CO molecule.

关键词： catalyst nanostructure metal adsorption oxidation reaction adsorption energy

Coxsackievirus B3 HFMD animal models in Syrian hamster and rhesus monkey

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Virologica Sinica 2024年第2期39卷 290-300页

作者： Suqin Duan Wei Zhang Yongjie Li Yanyan Li Yuan Zhao Weihua Jin Quan Liu Mingxue Li Wenting Sun Lixiong Chen Hongjie Xu Jie Tang Jinghan Hou Zijun Deng Fengmei Yang Shaohui Ma Zhanlong He Institute of medical biology Chinese Academy of Medical Sciences Medical Primate Research Center Peking Union Medical CollegeYunnan Key Laboratory of Vaccine Research Development on Severe Infectious DiseaseKunming650118China

Coxsackievirus B3(CVB3)is the pathogen causing hand,foot and mouth disease(HFMD),which manifests across a spectrum of clinical severity from mild to severe.However,CVB3-infected mouse models mainly demonstrate viral myocarditis and pancreatitis,failing to replicate human HFMD symptoms.Although several enteroviruses have been evaluated in Syrian hamsters and rhesus monkeys,there is no comprehensive data on CVB3.In this study,we have first tested the susceptibility of Syrian hamsters to CVB3 infection via different routes.The results showed that Syrian hamsters were successfully infected with CVB3 by intraperitoneal injection or nasal drip,leading to nasopharyngeal colonization,acute severe pathological injury,and typical HFMD symptoms.Notably,the nasal drip group exhibited a longer viral excretion cycle and more severe pathological damage.In the subsequent study,rhesus monkeys infected with CVB3 through nasal drips also presented signs of HFMD symptoms,viral excretion,serum antibody conversion,viral nucleic acids and antigens,and the specific organ damages,particularly in the heart.Surprisingly,there were no significant differences in myocardial enzyme levels,and the clinical symptoms resembled those often associated with common,mild infections.In summary,the study successfully developed severe Syrian hamsters and mild rhesus monkey models for CVB3-induced HFMD.These models could serve as a basis for understanding the disease pathogenesis,conducting pre-trial prevention and evaluation,and implementing post-exposure intervention.

关键词： Coxsackievirus B3(CVB3) Hand foot and mouth disease(HFMD) Animal models Syrian hamster Rhesus monkey

Splicing factor SF3B3,a NS5-binding protein,restricts ZIKV infection by targeting GCH1

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Virologica Sinica 2023年第2期38卷 222-232页

作者： Tanxiu Chen Hao Yang Penghui Liu Moliduer Hamiti Xintian Zhang Yi Xu Wenqi Quan Yong Zhang Wenhai Yu Li Jiao Tingfu Du Juemin Xi Bin Yin Wei Zhou Shuaiyao Lu Xiaozhong Peng Institute of medical biology Chinese Academy of Medical Sciences and Peking Union Medical CollegeKunming650031China Department of Science and Education Jiangxi Key Laboratory of Translational Cancer ResearchJiangxi Cancer HospitalNanchang330029China The State Key Laboratory of medical Molecular biology Department of Molecular Biology and BiochemistryInstitute of Basic Medical SciencesMedical Primate Research CenterNeuroscience CenterChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100730China

Zika virus(ZIKV),a positive-sense single-stranded RNA virus,causes congenital ZIKV syndrome in children and Guillain-Barre Syndrome(GBS)in adults.ZIKV expresses nonstructural protein 5(NS5),a large protein that is essential for viral replication.ZIKV NS5 confers the ability to evade interferon(IFN)signalling;however,the exact mechanism remains unclear.In this study,we employed affinity pull-down and liquid chromatography-tandem mass spectrometry(LC-MS/MS)analyses and found that splicing factor 3b subunit 3(SF3B3)is associated with the NS5-Flag pull-down complex through interaction with NS5.Functional assays showed that SF3B3 overexpression inhibited ZIKV replication by promoting IFN-stimulated gene(ISG)expression whereas silencing of SF3B3 inhibited expression of ISGs to promote ZIKV replication.GTP cyclohydrolase I(GCH1)is the first and ratelimiting enzyme in tetrahydrobiopterin(BH4)biosynthesis.NS5 upregulates the expression of GCH1 during ZIKV infection.And GCH1 marginally promoted ZIKV replication via the IFN pathway.Additionally,GCH1 expression is related to the regulation of SF3B3.Overexpression of the SF3B3 protein effectively reduced GCH1 protein levels,whereas SF3B3 knockdown increased its levels.These findings indicated that ZIKV NS5 binding protein SF3B3 contributed to the host immune response against ZIKV replication by modulating the expression of GCH1.

关键词： Nonstructural protein 5(NS5) Splicing factor 3b subunit 3(SF3B3) GTP cyclohydrolase I(GCH1) IFN-stimulated gene(ISGs) IFN signalling Pathway

A pilot study of the relative number of circulating tumor cells and leukocytes containing actin-binding proteins in head and neck cancer patients

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The Journal of Biomedical research 2023年第3期37卷 213-224页

作者： Gelena Kakurina Marina Stakheeva Elena Sereda Evgenia Sidenko Olga Cheremisina Evgeny Choinzonov Irina Kondakova Cancer research Institute Tomsk National Research Medical CenterRussian Academy of SciencesTomsk 634009Russia Department of Biochemistry and Molecular biology Faculty of Medicine and BiologySiberian State Medical UniversityTomsk 634050Russia.

Circulating tumor cells(CTCs)play an important role in tumor metastases,which is positively correlated with an increased risk of death.Actin-binding proteins,including cofilin(CFL1),profilin 1(PFN1),and adenylate cyclase-associated protein 1(CAP1),are thought to be involved in tumor cell motility and metastasis,specifically in head and neck squamous cell carcinoma(HNSCC).However,currently,there are no published studies on CFL1,PFN1,and CAP1 in CTCs and leukocytes in HNSCC patients.We assessed serum levels of CFL1,PFN1,and CAP1 and the number of CTCs and leukocytes containing these proteins in blood from 31 HNSCC patients(T1-4N0-2M0).The analysis used flow cytometry and an enzyme-linked immunosorbent assay kit.We found that CAP1+CTCs and CAP1+leukocyte subpopulations were prevalent in these HNSCC patient samples,while the prevalence rates of CFL1+and PFN1+CTCs were relatively low.Patients with stage T2-4N1-2M0 had CFL1+and PFN1+CTCs with an elevated PFN1 serum level,compared with the T1-3N0M0 group.In summary,the PFN1 serum level and the relative number of PFN1+CD326+CTCs could be valuable prognostic markers for HNSCC metastases.The current study is the first to obtain data regarding the contents of actin-binding proteins(ABPs)in CTCs,and leukocytes in blood from HNSCC patients.This is also the first to assess the relationship between the number of CTCs subgroups and disease characteristics.

关键词： head and neck squamous cell carcinoma metastasis circulating tumor cells actin-binding proteins adenylyl cyclase-associated protein 1

Melatonin, circadian rhythms and glaucoma: current perspective

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Neural Regeneration research 2022年第8期17卷 1759-1760页

作者： Denis Gubin Dietmar Weinert Chronobiology&Chronomedicine Laboratory Medical UniversityTyumen/Department of BiologyMedical UniversityTyumen/Tyumen Cardiology Research CenterTomsk National Research Medical CenterRussian Academy of ScienceTomskRussia Institute of biology/Zoology Martin Luther UniversityHalle-WittenbergGermany

Primary open-angle glaucoma as a causal factor for circadian disruption:Living by the clock,in alignment with external time cues is an important condition for human health and well-being.Periodic changes in the ambient light serve as a key factor to synchronize the endogenously generated circadian rhythms.The retina perceives the photic signals and transmits them to the central body clock,the suprachiasmatic nuclei(SCN),via the retinohypothalamic tract.

关键词： ambient alignment Periodic

Costunolide covalently targets NACHT domain of NLRP3 to inhibit inflammasome activation and alleviate NLRP3-driven inflammatory diseases

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Acta Pharmaceutica Sinica B 2023年第2期13卷 678-693页

作者： Haowen Xu Jiahao Chen Pan Chen Weifeng Li Jingjing Shao Shanshan Hong Yi Wang Lingfeng Chen Wu Luo Guang Liang Chemical biology research center School of Pharmaceutical SciencesWenzhou Medical UniversityWenzhou 325035China School of Pharmaceutical Sciences Hangzhou Medical CollegeHangzhou 311399China medical research center the First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000China

The NLRP3 inflammasome’s core and most specific protein,NLRP3,has a variety of functions in inflammation-driven diseases.Costunolide(COS)is the major active ingredient of the traditional Chinese medicinal herb Saussurea lappa and has anti-inflammatory activity,but the principal mechanism and molecular target of COS remain unclear.Here,we show that COS covalently binds to cysteine 598 in NACHT domain of NLRP3,altering the ATPase activity and assembly of NLRP3 inflammasome.We declare COS’s great anti-inflammasome efficacy in macrophages and disease models of gouty arthritis and ulcerative colitis via inhibiting NLRP3 inflammasome activation.We also reveal that theα-methylene-γ-butyrolactone motif in sesquiterpene lactone is the certain active group in inhibiting NLRP3 activation.Taken together,NLRP3 is identified as a direct target of COS for its anti-inflammasome activity.COS,especially theα-methylene-γ-butyrolactone motif in COS structure,might be used to design and produce novel NLRP3 inhibitors as a lead compound.

关键词： Costunolide NLRP3 inflammasome Inflammation Molecular target NACHT domain α-Methylene-γ-butyrolactone Gouty arthritis Ulcerative colitis

Critical involvement of circular RNAs in virus-associated cancers

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Genes & Diseases 2023年第6期10卷 2296-2305页

作者： Arghavan Zebardast Tayebeh Latifi Moein shirzad Golnaz Goodarzi Saeed Ebrahimi Fana Sadra Samavarchi Tehrani Yousef Yahyapour Department of Virology School of Public HealthTehran University of Medical SciencesTehran ***Iran Cellular and Molecular biology research center Health Research InstituteBabol University of Medical SciencesBabol 47176Iran Department of Clinical Biochemistry School of MedicineTehran University of Medical SciencesTehran ***Iran Student Scientific research center Tehran University of Medical SciencesTehran ***Iran Infectious Diseases and Tropical Medicine research center Health Research InstituteBabol University of Medical SciencesBabol 47176Iran

Virus-related cancer is cancer where viral infection leads to the malignant transformation of the host's infected cells. Seven viruses (e.g., human papillomavirus (HPV), Epstein–Barr virus (EBV), Kaposi's sarcoma herpesvirus (KSHV), Hepatitis B virus (HBV), Hepatitis C virus (HCV), Human T-lymphotropic virus (HTLV), and Merkel cell polyomavirus (MCV)) that infect humans have been identified as an oncogene and have been associated with several human malignancies. Recently, growing attention has been attracted to exploring the pathogenesis of virus-related cancers. One of the most mysterious molecules involved in carcinogenesis and progression of virus-related cancers is circular RNAs (circRNA). These emerging non-coding RNAs (ncRNAs), due to the absence of 5′ and 3′ ends, have high stability than linear RNAs and are found in some species across the eukaryotic organisms. Compelling evidence has revealed that viruses also encode a repertoire of circRNAs, as well as dysregulation of these viral circRNAs play a critical role in the pathogenesis and progression of different types of virus-related cancers. Therefore, understanding the exact role and function of the virally encoded circRNAs with virus-associated cancers will open a new road for increasing our knowledge about the RNA world. Hence, in this review, we will focus on emerging roles of virus-encoded circRNAs in multiple cancers, including cervical cancer, gastric cancer, Merkel cell carcinoma, nasopharyngeal carcinoma, Kaposi cancer, and liver cancer.

关键词： Cancer Circular RNA Neoplasms Oncogenes Viruses

Two novel sets of UiO-66@metal oxide/graphene oxide Z-scheme heterojunction:Insight into tetracycline and malathion photodegradation

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Journal of Environmental Sciences 2020年第5期32卷 222-236页

作者： Hanieh Fakhri Hasan Bagheri Chemical Injuries research center Systems Biology and Poisonings InstituteBaqiyatallah University of Medical SciencesTehranIran

Nowadays,widespread researches have been focused on the development of effective photocatalysts to remove pollutants of the aquatic system.In accordance with the universal studies,two new sets of UiO-66@metal oxide(including ZnO and TiO2)/graphene oxide heterojunctions were synthesized for photodegradation of aromatic(tetracycline)and nonaromatic(malathion)pollutants which are challenging cases in the environment.The dosage of the photocatalyst,pH of the solution,the type of metal oxide,and the presence of various scavengers are assayed parameters in this work.In the optimum condition,maximum photodegradation efficiency is achieved in 90 min for tetracycline(81%)and malathion(100%)by the UiO-66@ZnO/graphene oxide.The superior separation of charge carriers by Z-scheme mechanism,excellent electron mobility on layers of graphene oxide and high surface area are factors that enhanced the efficiency.Furthermore,in comparison with pure UiO-66,the band gaps belong to heterojunctions revealed a red shift in the absorption edge,which can be responsible for more expand adsorption of the solar spectrum.Total organic carbon analysis verified the decontamination of these pollutants in the solution.The produced main intermediates during the photocatalytic process were identified and the possible degradation pathway proposed.In general,the superior photocatalytic activity suggests that these designed photocatalysts can be a promising choice for having a clean future.

关键词： Metal-organic framework UiO-66 Photodegradation Malathion Tetracycline Z-scheme mechanism

Cell therapy for the treatment of reproductive diseases and infertility:an overview from the mechanism to the clinic alongside diagnostic methods

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Frontiers of Medicine 2022年第6期16卷 827-858页

作者： Kosar Babaei Mohsen Aziminezhad Seyedeh Elham Norollahi Sogand Vahidi Ali Akbar Samadani Non-Communicable Disease research center Neyshabur University of Medical SciencesNeyshaburIran UMR INSERM U 1122 IGE-PCVInteractions Gène-Environment En Physiopathologie Cardiovascular UniversitéDe LorraineNancyFrance Cancer research center and Department of Immunology Semnan University of Medical SciencesSemnanIran Medical Biology Research Center Kermanshah University of Medical SciencesKermanshahIran Guilan Road Trauma research center Guilan University of Medical SciencesRashtIran

Infertility is experienced by 8%–12%of adults in their reproductive period globally and has become a prevalent concern.Besides routine therapeutic methods,stem cells are rapidly being examined as viable alternative therapies in regenerative medicine and translational investigation.Remarkable progress has been made in understanding the biology and purpose of stem cells.The affected pluripotent stem cells(iPSCs)and mesenchymal stem cells(MSCs)are further studied for their possible use in reproductive medicine,particularly for infertility induced by premature ovarian insufficiency and azoospermia.Accordingly,this study discusses current developments in the use of some kinds of MSCs such as adipose-derived stem cells,bone marrow stromal cells,umbilical cord MSCs,and menstrual blood MSCs.These methods have been used to manage ovarian and uterine disorders,and each technique presents a novel method for the therapy of infertility.

关键词： infertility stem cell therapy mesenchymal stem cells pluripotent stem cells