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Autophagy and mitophagy as potential therapeutic targets in diabetic heart condition:Harnessing the power of nanotheranostics

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Asian Journal of Pharmaceutical Sciences 2024年第3期19卷 79-101页

作者： Sagnik Nag Oishi Mitra Bhanu Maturi Simran Preet Kaur Ankita Saini Muskan Nama Soumik Roy Souvik Samanta Leena Chacko Rohan Dutta Suresh Babu Sayana Vetriselvan Subramaniyan Jasvinder Singh Bhatti Ramesh Kandimalla Jeffrey Cheah School of Medicine and Health Sciences Monash University MalaysiaJalan Lagoon Selatan47500 Bandar SunwaySelangorMalaysia Department of Bio-Sciences School of Bio-Sciences&Technology(SBST)Vellore Institute of Technology(VIT)Tiruvalam RoadVellore 632014Tamil NaduIndia Department of Internal Medicine University of Alabama at BirminghamBirminghamALUSA Department of Microbiology University of Delhi(South Campus)Benito Juarez RoadNew Delhi 110021India Department of biotechnology Indian Institute of TechnologyHyderabad(IIT-H)SangareddyTelangana 502284India BioAnalytical lab Meso Scale Discovery1601 Research BlvdRockvilleMDUSA Department of Pharmacology Government Medical CollegeSuryapetTelanganaIndia lab.ratory of Translational Medicine and Nanotherapeutics Department of Human Genetics and Molecular MedicineSchool of Health SciencesCentral University of PunjabBathindaIndia Department of Biochemistry Kakatiya Medical CollegeWarangal 506007India

Autophagy and mitophagy pose unresolved challenges in understanding the pathology of diabetic heart condition(DHC),which encompasses a complex range of cardiovascular issues linked to diabetes and associated *** significant progress in reducing mortality rates from cardiovascular diseases(CVDs),heart failure remains a major cause of increased morbidity among diabetic *** cellular processes are essential for maintaining cellular balance and removing damaged or dysfunctional components,and their involvement in the development of diabetic heart disease makes them attractive targets for diagnosis and *** a variety of conventional diagnostic and therapeutic strategies are availab.e,DHC continues to present a significant ***-of-care diagnostics,supported by nanobiosensing techniques,offer a promising alternative for these complex *** conventional medications have been widely used in DHC patients,they raise several concerns regarding various physiological *** medicine places great emphasis on the application of nanotechnology to target autophagy and mitophagy in DHC,offering a promising approach to deliver drugs beyond the limitations of traditional *** article aims to explore the potential connections between autophagy,mitophagy and DHC,while also discussing the promise of nanotechnology-based theranostic interventions that specifically target these molecular pathways.

关键词： Autophagy Diabetes Diabetic heart condition Mitophagy Nanotheranostics Nanomedicine

Paper Analytical Device for the Colourimetric Detection of Alkaline Phosphatase in Serum and Saliva

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Journal of Analysis and Testing 2024年第3期8卷 385-394页

作者： D.Alageswari A.LakshmiDevi P.E.Resmi P.V.Suneesh AarathiPradeep T.G.SatheeshBabu Department of Chemistry Amrita School of Physical Sciences Coimbatore Amrita Vishwa Vidyapeetham Amrita Biosensor Research lab. Amrita School of Engineering Coimbatore Amrita Vishwa Vidyapeetham Amrita Biomedical Engineering Centre Amrita School of Engineering Coimbatore Amrita Vishwa Vidyapeetham

A paper analytical device (PAD) was developed for the colourimetric estimation of alkaline phosphatase (ALP). ALP catalyzes the hydrolysis of disodium phenyl phosphate (DSPP) to phenol, which then undergoes oxidative coupling with4-aminoantipyrine (4-AAP) producing a reddish brown-coloured quinone imine. The colourimetric reaction tested in solution has been translated onto a PAD for the point of care (PoC) testing of ALP. A uniform colour was obtained in 25 min with the introduction of ALP onto the reagent drop cast paper device. The images obtained by scanning the PAD were processed using ImageJ software. The colour intensity obtained by image processing was proportional to the concentration of ALP present in the sample. ALP in serum and saliva samples were tested using the PAD in the range of 0 to 528 U/L and0 to 187 U/L, respectively. The interference studies revealed that the PAD was selective to ALP in the presence of other biomolecules. The PAD exhibited a limit of detection (LOD) of 3.3 U/L and a sensitivity of 48.36 a.u/Log(U/L) for serum ALP and LOD of 1.69 U/L and a sensitivity of 28.5 a.u/Log (U/L) for salivary ALP. The PAD showed good agreement with the clinical methods for real samples.

关键词： Alkaline phosphatase Colourimetry ImageJ Paper analytical device Point of care testing

The Expression and Location of Midkine in Gastric Carcinomas of Chinese Patients

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Cellular & Molecular Immunology 2007年第2期4卷 135-140页

作者： Yaling Huang Guochun Cao Hui Wang Qingling Wang Yayi Hou Immunology and Reproduction Biology lab Medical School ＆ State Key Laboratory of Pharmaceutical Biotechnology Nanjing University Nanjing 210093 China

Midkine （MK）, a heparin-binding growth factor, can regulate cell growth, survival and differentiation. MK is expressed at high levels in a variety of human carcinomas. Recently, the urine and serum MK concentration was analyzed in gastric cancer patient. However, the association of the cytokine mRNA expression with the categorical clinicopathological variables of the tumors and the location of its protein expression in the tumor tissues are still elusive. MK mRNA expression from the surgically resected specimens of healthy gastric tissues （9 cases）, gastric cancer tissues and the matched non-cancerons tissues adjacent to the cancer （37 cases） were assessed by reverse transcriptase-polymerase chain reaction （RT-PCR） and real-time PCR. Immunohistochemical analysis was performed to locate MK in gastric cancer. The expression of MK mRNA in gastric cancer was much higher in tumor tissues than that in the non-cancerons tissues and control tissue samples. And its expression was significantly associated with the pTNM stage and distant metastasis, but not with the differentiation grade, tumor size and nodal involvement. MK protein was ubiquitous in the tumor, especially in the adenoid part of tumors. In addition, it was found in the cytoplasm of tumor cells and highly concentrated in nucleus and nucleolns. The expression level and location of MK in gastric tumor tissues of Chinese Patients may be related to the tumor genesis and progression. Further study is necessary on the mechanism of MK in gastric tumorigenesis and tumor growth.

关键词： midkine gastric cancer RT-PCR real-time PCR immunohistochemical analysis

Lipid Oxidation and Histamine Production in Atlantic Mackerel（Scomber scombrus） Versus Time and Mode of Conservation

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Journal of Life Sciences 2012年第7期6卷 713-720页

作者： Hanane Oucif Smail Ali-Mehidi Sidi-Mohamed El-Amine Abi-Ayad lab. of Aquaculture and Bioremediation Dpt. of Biotechnology University of Es-Sdnia Oran 31000 Algeria

Lipids oxidation, histamine production and quality loss were studied according to storage time and temperature （ambient temperature （Tamb） 26 ℃, 4 ℃ and -18℃） in Atlantic mackerel （Scomber scombrus）. Muscle pH, hydrolysis of phospholipids, content of primary （hydroperoxides）, secondary lipid oxidation products （TBARS） and histamine were determined and compared with a sensory assessment. Atlantic mackerel is sensory acceptable, less than 24 hours at Tamb, for up to 3 days at 4 ℃ and 3 months at-18 ℃. Evolution of biochemical parameters with storage time and temperature showed significant differences （P 〈 0.05）. Muscle pH increased from 5.99 to 6.13 at Tamb, to 6.23 at 4 ℃ and to 6.04 at -18 ℃. The highest content of TBARS is associated with a decrease in phospholipids and hydroperoxides contents and highest levels of sensory alteration. Histamine content exceeded the limit recommended by the Trade Algerian Ministry （10 mg/100g）, after 24 hours at Tamb, 5 days of storage at 4 ~C only. Therefore, freezing storage has a preserving effect on lipid damage and histamine production and seems the best means of storage; if these species are not consumed during the two days following capture. Moreover, monitoring histamine production is more useful as sanitary index rather than spoilage parameter and the strategy used for measuring kinetic of lipid oxidation appear pertinent for determining the degree of oxidation.

关键词： Conservation histamine hydroperoxides phospholipids temperature TBARS Scomber scombrus.

Comparison of Antioxidant Activities in Black Soybean Preparations Fermented with Various Microorganisms

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Agricultural Sciences in China 2010年第7期9卷 1065-1071页

作者： QIN Yao JIN Xiao-nan Park Heui Dong Department of Food Science and Technology Kyungpook National University Daegu 702-701 Korea Applied Plant Stress Biology lab.Divison of biotechnology/College of Life Sciences and biotechnology Korea University Seoul 136-713 Korea

Black soybeans [Glycine max（L.） Merrill] were fermented with various GRAS（generally recognized as safe） microorganisms,including Aspergillus sp.,Bacillus *** yeast（Issatchenkia orientalis） at 30°C for 3 *** antioxidant contents,including total phenolics and total flavonoids,and the antioxidant activities of 1,1-diphenyl-2-picrylhydrazine（DPPH）,ferric reducing antioxidant power（FRAP） and *** were analyzed in preparations of fermented black *** cultures proved to yield significant levels of antioxidants compared with non-fermented cultures（P 〈 0.05）.Fermented black soybean preparations possessed antioxidant activities could be attributed to the total phenolics and flavonoids present.

关键词： antioxidant activity black soybean microorganisms fermentation

Pectate lyase is a factor in the adaptability for Heterodera glycines infecting tobacco

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Journal of Integrative Agriculture 2019年第3期18卷 618-626页

作者： TIAN Zhong-ling SHI Hong-li Munawar Maria ZHENG Jing-wu lab.ratory of Plant Nematology Institute of BiotechnologyCollege of Agriculture&BiotechnologyZhejiang University School of Resource and Environmental Sciences Henan Institute of Science and Technology Key lab.of Molecular Biology of Crop Pathogens and Insects Ministry of Agriculture

The soybean cyst nematode, Heterodeara glycines, is a serious pathogen of soybean, and reported to be the host of a wide range of Fabaceae. In the present study, the host specificity and reproductivity of two populations of H. glycines collected from soybean and tobacco were identified and characterized. The comparative identity between β-1,4-endoglucanase, pectate lyase and chorismate mutase of H. glycines parasitizing on soybean and tobacco were 99, 97 and 98%, respectively. The qR T-PCR analysis indicated that the expression of pectate lyase 2 gene was significantly higher in second-stage juveniles of H. glycines Henan population parasitizing on tobacco than that of H. glycines Shanxi population parasitizing on soybean. In addition, the pectic acid content of cell wall was significantly higher(45%) in the roots of tobacco than the roots of soybean. Our results indicate that the changes in transcript parasitism genes may be a result of long-term evolution illustrating how a plant-parasitic nematode adapts to the host environment for optimal infestation and survival.

关键词： s: Heterodera glycines tobacco parasitism genes β-1,4-endoglucanase pectate lyase chorismate mutase pectic acid

Investigating Interaction Between Biochanin A and Human Serum Albumin by Multi-spectroscopic and Molecular Simulation Methods

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Transactions of Tianjin University 2017年第4期23卷 325-333页

作者： Zhaohui Xue Aiqing Cheng Yanni Li Wancong Yu Xiaohong Kou School of Chemical Engineering and Technology Tianjin University Medical Plant lab Tianjin Research Center of Agricultural Biotechnology

Biochanin A (BCA), the most abundant isoflavone in chickpeas, presents a wide range of biological activities, such as hypolipidaemic, anti-oxidative, anti-proliferative, and estrogen-like effects. We investigated the interaction between BCA and human serum albumin (HSA) via several techniques. UV–Vis absorption spectroscopy verified the conformational variation of HSA after BCA addition, and fluorescence spectroscopy revealed the relevant binding parameters. Circular dichroism spectroscopy was used to estimate the secondary structural changes of HSA with and without BCA. Molecular docking and dynamics simulations were then applied to study the characteristics of HSA with BCA. Energy decomposition analysis was used to prove that Trp214 in subdomain IIA of HSA is the most likely binding site of BCA. Van der Waals forces and hydrophobic interactions may play important roles during the binding process. All of our results showed that BCA presents significant binding affinity to HSA, thus confirming that the role of HSA has as an efficient transporter of biomolecules. © 2017, Tianjin University and Springer-Verlag Berlin Heidelberg.

关键词： Absorption spectroscopy Binding energy Bins Body fluids Dichroism Flavonoids Fluorescence spectroscopy Hydrophobicity Molecular biology Molecular dynamics Van der Waals forces

An overview on application of phage display technique in immunological studies

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Asian Pacific Journal of Tropical Biomedicine 2017年第7期7卷 599-602页

作者： Abbas Rami Mahdi Behdani Najmeh Yardehnavi Mahdi Habibi-Anbouhi Fatemeh Kazemi-Lomedasht biotechnology Research Center Venom&Biotherapeutics Molecules Lab. Pasteur Institute of Iran Tehran Iran Medical Cellular and Molecular Research Center Golestan University of Medical Sciences Gorgan Iran Department of Medical biotechnology Faculty of Advanced Medical Technologies Golestan University of Medical Sciences Gorgan Iran National Cell Bank of Iran Pasteur Institute of Iran Tehran Iran

Phage display is very strong technique in drug discovery and development. Phage display has many applications in improving the immunological studies. Development of monoclonal antibody, peptides, peptidomimetics and epitope mapping are main application of phage display. Selection of monoclonal antibody or peptides that are displayed on the surface of the phages can be occurred through biopanning process. In biopanning process phage library is incubated with antigen and particular phages can be identified and isolated. Increasing the stringency in the biopanning rounds can be help to select phages with high affinity and specificity. Here, we describe an overview of phage display application with focusing on monoclonal antibody production and epitope mapping.

关键词： Phage display Antibody Epitope mapping Immunology Antibody library Peptide library

Synthesis,Crystal Structure and Antimicrobial Study of N,N,N'-Tris(3-aminopropyl)ethylenediamine Copper(Ⅱ) Diperchlorate

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Chinese Journal of Structural Chemistry 2007年第1期26卷 121-125页

作者：许兴友许同桃高健王大奇陆路德徐国想 Key marine biotechnology lab.ratary Huaihai Institute of Technology Materials Chemistry lab.ratory Nanjing University of Science&Technology Department of Chemical Engineering Lianyungang Technical College College of Chemistry and Chemical Engineering Liaocheng University

The title compound [Cu（C11H29N5）]（ClO4）2 was synthesized and characterized by elemental analysis, IR and X-ray single-crystal diffraction. The compound crystallizes in the monoclinic system, space group P21/n with α = 9.405（2）, b = 14.495（3）, c = 14.715（3） A, β= 90.262（3）°, V=2006.1（7） A^3,M,=493.83,Z=4,F）（000）= 1028,Dc= 1.635 g/cm^3, T= 298（2） K,μ= 1.403 mm^-1 and = 0.71073 A. The was refined to R = 0.0508 and wR = 0. 1250 for 2293 observed reflections with I 〉 2σ（I）. The Cu（Ⅱ） atom possesses a five-coordtinate CuN5 environment which shows a slightly distorted square-pyramidal geonetry. Antimicrobial activity study found that the complex is active against Salmonella species, Staphylococcus aureu, Bacillus pumilus and Bacillus coliforms.

关键词： N,N,N'-tris(3-aminopropyl)ethylenediamine copper compound crystal antimicrobial activity