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Lycorine derivative effectively inhibits the replication of coronaviruses both in vitro and in vivo

hLife 2024年第2期2卷 75-87页

作者： Liang Shen Jianzhong Zhao Ying Xia Junjie Lu Jiali Sun Jian Tang Hui Xing Lijuan Yin Yang Yang Chunhua Wang Department of Clinical lab.ratory Xiangyang Central HospitalAffiliated Hospital of Hubei University of Arts and ScienceHubeiChina Shenzhen Key lab.ratory of Pathogen and Immunity National Clinical Research Center for Infectious DiseaseState Key Discipline of Infectious DiseaseShenzhen Third People’s HospitalSecond Hospital Affiliated to Southern University of Science and TechnologyGuangdongChina Department of Clinical lab.ratory Xiangyang No.1 People’s HospitalHubei University of MedicineHubeiChina State Key lab.ratory of Food Nutrition and Safety Key Laboratory of Industrial MicrobiologyMinistry of EducationTianjin Key Laboratory of Industry MicrobiologyNational and Local United Engineering Lab of Metabolic Control Fermentation TechnologyChina International Science and Technology Cooperation Base of Food Nutrition/Safety and Medicinal ChemistryCollege of BiotechnologyTianjin University of Science&TechnologyTianjinChina

The established and ongoing prevalence of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and seasonal human coronaviruses(HCoV)like HCoV-OC43,HCoV-NL63,and HCoV-229E,pose a continuous threat to public ***,it is urgently needed to explore antiviral drugs with broad-spectrum anti-coronavirus *** previous studies have revealed that lycorine is a potent broad-spectrum anti-coronavirus drug,a natural alkaloid extracted from Amaryllidaceae with various pharmacological and microbiological ***,it is unsafe to directly use lycorine as a clinical antiviral drug due to the cytotoxicity and induction of cell *** this study,a series of lycorine derivatives were designed and *** of them,named Ly-8,was found to effectively inhibit the replication of different coronavirus strains in vitro,including ***,Ly-8 was also shown to effectively inhibit HCoVOC43 replication in the central nervous system,and provide effective protection against HCoV-OC43 infection in mice with low drug ***,Ly-8-resistant mutants were not observed during the 30 times sequential passages in cell ***,these findings suggest that Ly-8 may be a potential candidate drug for the future development of broad-spectrum anti-coronavirus drugs.

关键词： broad-spectrum antiviral drug lycorine derivative Ly-8

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Degradation of chlorpyrifos in lab.ratory soil and its impact on soil microbial functional diversity

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Journal of Environmental Sciences 2009年第3期21卷 380-386页

作者： FANG Hua YU Yunlong CHU Xiaoqiang WANG Xiuguo YANG Xiaoe YU Jingquan Department of Plant Protection College of Agriculture & Biotechnology Zhejiang University Hangzhou 310029 China MOE Key lab.of Environment Remediation & Ecosystem Health College of Environmental and Resource Sciences Zhejiang University Hangzhou 310029 China Department of Horticulture College of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China

Degradation of chlorpyrifos at different concentrations in soil and its impact on soil microbial functional diversity were investigated under lab.ratory condition. The degradation half-live of chlorpyrifos at levels of 4, 8, and 12 mg/kg in soil were calculated to be 14.3, 16.7, and 18.0 d, respectively. The Biolog study showed that the average well color development （AWCD） in soils was significantly （P 〈 0.05） inhibited by chlorpyrifos within the first two weeks and thereafter recovered to a similar level as the control. A similar variation in the diversity indices （Simpson index lID and McIntosh index U） was observed, but no significant difference among the values of the Shannon-Wiener index H＇ was found in chlorpyrifos-treated soils. With an increasing chlorpyrifos concentration, the half-life of chlorpyrifos was significantly （P ≤ 0.05） extended and its inhibitory effect on soil microorganisms was aggravated. It is concluded that chlorpyrifos residues in soil had a temporary or short-term inhibitory effect on soil microbial functional diversity.

关键词： Biolog chlorpyrifos community-level physiological profile microbial functional diversity

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A Three-Dimensional (3D) Environment to Maintain the Integrity of Mouse Testicular Can Cause the Occurrence of Meiosis

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Journal of Integrative Agriculture 2013年第8期12卷 1481-1488页

作者： CHU Zhi-li LIU Chao BAI Yao-fu ZHU Hai-jing HU Yue HUA Jin-lian College of Veterinary Medicine Shaanxi Center of Stem Cells Engineering & Technology/Key Lab for Animal BiotechnologyMinistry of Agriculture/Northwest A&F University

Adhesions between different cells and extracellular matrix have been studied extensively in vitro, but little is known about their functions in testicular tissue counterparts. Spermatogonia and their companion somatic cells maintain a close association throughout spermatogenesis and this association is necessary for normal spermatogenesis. In order to keep the relative integrity of the testicular tissues, and to detect the development in vitro, culture testicular tissues in a three- dimensional （3D） agarose matrix was examined. Testicular tissues isolated from 6.5 d postpartum （dpp） mouse were cultured on the top of the matrix for 26 d with a medium height up to 4/5 of the 3D agarose matrix. The results showed that in this 3D culture environment, each type of testicular cells kept the same structure, localization and function as in vivo and might be more biologically relevant to living organisms. After culture, germ cell marker VASA and meiosis markers DAZL and SCP3 showed typical positive analysed by immunofluorescence staining and RT-PCR. It demonstrated that this 3D culture system was able to maintain the number of germ cells and promote the meiosis initiation of male germ cells.

关键词： three-dimensional culture (3D) meiosis organ culture mouse

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Synthesis,Structure and Luminescent Properties of a Two-dimensional Cobalt(Ⅱ) Coordination Polymer Based on 2-Thiobarbituric Acid

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Chinese Journal of Structural Chemistry 2017年第7期36卷 1210-1215页

作者：王超周梦思杨丽佳梁凯朱智甲陈珍霞 College of Chemistry Chemical Engineering and Biotechnology Donghua University Key lab.of Eco-textile Ministry of Education Donghua University Department of Chemistry Fudan University

A two-dimensional coordination polymer [Co（H2tba）2（DMF）2]n 1 （H2tba = 2-thiobarbituric acid anion, DMF = N,N-dimethylformamide） has been synthesized by CoCl2？6H2O with the 2-thiobarbituric acid ligands under solvothermal conditions. Compound 1 was further characterized by FTIR, elemental analysis, TGA and powder XRD techniques. Single-crystal X-ray diffraction studies revealed that the title compound crystallizes in the monoclinic space group Pbca with a = 12.5948（12）, b = 11.4269（11）, c = 14.8939（15） , V = 2143.5（4）？3, Z = 4, Dc = 1.523 g·cm-3, F（000） = 1012, μ = 1.038 mm-1, R = 0.0358 and wR2 = 0.0888 （I 〉 2σ（I））. Single-crystal X-ray diffraction analysis reveals that 1 is the first two-dimensional noninterpenetrating （4, 4） net coordination polymer based on the 2-thiobarbituric acid. Coordination polymer 1 exhibits two luminescent emission spectra at 477 and 489 nm with the excitation peak at 273 nm.

关键词： solvothermal synthesis coordination polymers luminescence

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Induction of nucleoside phosphorylase in Enterobacter aerogenes and enzymatic synthesis of adenine arabinoside

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Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2008年第7期9卷 520-526页

作者： Xiao-kun WEI Qing-bao DING Lu ZHANG Yong-li GUO Lin OU Chang-lu WANG College of biotechnology Tianjin University of Science and Technology Tianjin 300457 China State Key lab.of Bioreactor Engineering East China University of Science and Technology Shanghai 200237 China Shanghai Weiping Bioscience and biotechnology Co. Ltd. Shanghai 200237 China

Nucleoside phosphorylases (NPases) were found to be induced in Enterobacter aerogenes DGO-04, and cytidine and cytidine 5′-monophosphate (CMP) were the best inducers. Five mmol/L to fifteen mmol/L cytidine or CMP could distinctly increase the activities of purine nucleoside phosphorylase (PNPase), uridine phosphorylase (UPase) and thymidine phosphorylase (TPase) when they were added into medium from 0 to 8 h. In the process of enzymatic synthesis of adenine arabinoside from adenine and uracil arabinoside with wet cells of Enterobacter aerogenes DGO-04 induced by cytidine or CMP, the reaction time could be shortened from 36 to 6 h. After enzymatic reaction the activity of NPase in the cells induced remained higher than that in the cells uninduced.

关键词： Nucleoside phosphorylase (NPase) Enterobacter aerogenes Cytidine Cytidine 5'-monophosphate (CMP) Adenine arabinoside

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Morphological and molecular characterization of the rice root-knot nematode, Meloidogyne graminicola, Golden and Birchfeild, 1965 occurring in Zhejiang, China

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Journal of Integrative Agriculture 2018年第12期17卷 2724-2733页

作者： TIAN Zhong-ling Munawar Maria Eda Marie Barsalote Pablo Castillo ZHENG Jing-wu lab.ratory of Plant Nematology Institute of Biotechnology College of Agriculture and Biotechnology Zhejiang University Institute for Sustainable Agriculture(IAS) Spanish National Research Council (CSIC) Campus de Excelencia Internacional Agroalimentario Key lab.of Molecular Biology of Crop Pathogens and Insects Ministry of Agriculture

The rice root-knot nematode Meloidogyne graminicola is a severe pest of rice. In China, it was first reported from Hainan Province, and later from several other provinces. In the present study, a rice root-knot nematode population found from the rice cultivation areas of Zhejiang Province, China is characterized via molecular analysis using internal transcribed spacer(ITS) and cytochrome c oxidase subunit Ⅱ(coxⅡ)-16 S rRNA genes and scanning electron microscopy(SEM) observations of males and the second-stage juveniles. Morphometric data and molecular sequence comparisons for all M. graminicola populations occurring in China are also provided. The overall morphology of M. graminicola found in Zhejiang match well with the original description, though males have a slightly longer body and stylet, and a shorter tail, while the second-stage juvenile is also slightly longer than in the original description. This is the first report of M. graminicola from Zhejiang. Phylogenetic studies based on coxⅡ suggest that all the Chinese populations belong to Type B. This study expands knowledge of the increasing distribution and phylogenetic relationships of M. graminicola that occur in China.

关键词： Meloidogyne graminicola morphology morphometric molecular rice rDNA cytochrome c oxidase subunit Ⅱ (coxⅡ) China

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Application of response surface methodology in medium optimization for pyruvic acid production of Torulopsis glab.ata TP19 in batch fermentation

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Journal of Zhejiang University-Science B(Biomedicine & biotechnology) 2007年第2期8卷 98-104页

作者： ZHANG Jian GAO Nian-fa Tianjin Key lab.of lndustrial Microbiology College of Biotechnology Tianjin University of Science and Technology Tianjin 300457 China

Response surface methodology (RSM) was used to optimize the fermentation medium for enhancing pyruvic acid production by Torulopsis glab.ata TP19. In the first step of optimization, with Plackett-Burman design, ammonium sulfate, glucose and nicotinic acid were found to be the important factors affecting pyruvic acid production significantly. In the second step, a 23 full factorial central composite design and RSM were applied to determine the optimal concentration of each significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the critical components were obtained as follows: ammonium sulfate 0.7498 (10.75 g/L), glucose 0.9383 (109.38 g/L) and nicotinic acid 0.3633 (7.86 mg/L) with a predicted value of maximum pyruvic acid production of 42.2 g/L. Under the optimal conditions, the practical pyruvic acid production was 42.4 g/L. The determination coefficient (R2) was 0.9483, which ensures adequate credibility of the model. By scaling up fermentation from flask to jar fermentor, we obtained promising results.

关键词： Response surface methodology Torulopsis glab.ata Pyruvic acid Fermentation Medium optimization

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Immunoproteomics of membrane proteins of Shigella flexneri 2a 2457T

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World Journal of Gastroenterology 2005年第43期11卷 6880-6883页

作者： Tian-Yi Ying Jun-Jun Wang Heng-Liang Wang Er-Ling Feng Kai-Hua Wei Liu-Yu Huang Pei-Tang Huang Cui-Fen Huang Beijing Institute of biotechnology State Key Lab of Pathogen and Biosecurity Beijing 100071 China Beijing Institute of Pharmaceutical Chemistry Beijing 102205 China

AIM： To screen the immunogenic membrane proteins of Shigella Aexneri 2a 2457T. METHODS： The routine two-dimensional polyacrylamide gel electrophoresis （2-DE） and Western blotting were combined to screen immunogenic proteins of S. Aexneri 2a 2457T. Serum was gained from rabbits immunized with the same bacteria. Immunogenic spots were cut out from the polyacrylamide gel and digested by trypsin in-gel. Matrix-assisted laser desorption/ionization time of flight-mass spectrometry （MALDI-TOF-MS） was performed to determine the molecular weight of peptides. Electrospray ionization （ESI-MS/MS） was performed to determine the sequences of the interesting peptides. RESULTS： A total of 20 spots were successfully identified from Coomassie brilliant blue stained gels representing 13 protein entries, 5 known antigens and 8 novel antigens. A hypothetical protein （YaeT） was detected, which might be a candidate target of vaccine. CONCLUSION： Membrane proteins of S. flexneri 2a 2457T were successfully observed by 2-DE. Several known and novel antigens were identified by mass spectrum.

关键词： Shigella flexneri 2a 2457T Immunoproteomics Membrane proteins

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Deciphering fungal metabolon coupling tandem inverse-electrondemand Diels-Alder reaction and semipinacol rearrangement for the biosynthesis of spiro polycyclic alkaloids

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Science China Chemistry 2024年

作者： Shuai Liu Wen-Qiang Xu Ying-Tong Di Man-Cheng Tang Ding-Kang Chen Ming-Ming Cao Yao-Wen Chang Hong-Yu Tang Chun-Mao Yuan Jun-Bo Yang Zhi-Li Zuo Han Guo Zi-Fei Xu Ying Zeng Yun-Dong Wu Xiao-Jiang Hao Key lab.ratory of Phytochemistry and Natural Medicines (Chinese Academy of Sciences) Kunming Institute of Botany Chinese Academy of Sciences University of Chinese Academy of Sciences lab.of Computational Chemistry and Drug Design State Key Laboratory of Chemical Oncogenomics Peking University Shenzhen Graduate School Shenzhen Bay lab.ratory State Key lab.ratory of Microbial Metabolism School of Life Sciences & Biotechnology Shanghai Jiao Tong University State Key lab.ratory of Functions and Applications of Medicinal Plants Guizhou Medical University Molecular Biology Experimental Center Germplasm Bank of Wild Species in Southwest China Kunming Institute of Botany Chinese Academy of Sciences Yunnan Key lab.ratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences Research Unit of Chemical Biology of Natural Anti-Virus Products Chinese Academy of Medical Sciences

In the intricate process of natural product biosynthesis, a metabolon can enhance metabolic flux by associating sequential enzymes. A fungal metabolon, comprising of flavin-dependent monooxygenase SpeF and P450 monooxygenase SpeG, is identified in the biosynthesis of spiro polycyclic alkaloids(+)-notoamide B and its diastereomer(+)-versicolamide B. Using notoamide E as a substance, SpeF/SpeG metabolon can control the stereoselectivity of its 2,3-epoxidation, followed by hydrogen atom ion at C-17 to generate reactive epoxide tau-mA with dienyl iminium unit. Subsequently,(+)-notoamide B and(+)-versicolamide B are produced via tandem nonenzymatic inverse-electron-demand Diels-Alder reaction and semipinacol rearrangement. This provides the first example of metabolon in the biosynthesis of spiro-prenylated indole alkaloids.

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Expression pattern of GASA,downstream genes of DELLA,in Arabidopsis

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Chinese Science Bulletin 2008年第24期53卷 3839-3846页

作者： ZHANG ShengChun WANG XiaoJing College of Life Sciences South China Normal University Guangdong Provincial Key Lab of Biotechnology for Plant Development Guangzhou 510631 China

Separation and functional research of related components involved in gibberellins(GAs) signaling are important to clarify the mechanism of GA *** on the downstream components of DELLA,the key factor of the GA signaling pathway,is limited at ***(GA-Stimulated in Arabidopsis) family contains 15 genes usually regulated by GA in Arabidopsis *** GASA proteins have a cleavable signal peptide in N terminus and a conserved GASA domain including 12 cysteines in C ***-PCR analysis revealed that the expression of GASA4 and GASA6 were down-regulated,but GASA1 and GASA9 were up-regulated in the DELLA mutants,gai-t6 and rga-24,as well as the double mutant,consisting with the results that GASA4 and GASA6 were induced,but GASA1 and GASA9 were inhibited by exogenous *** addition,the expression patterns of other GASA genes were regulated by GA and ABA,separately or *** of GASA genes were expressed in roots,stems,leaves,flowers and developing *** gene driven by the promoters of GASA6,GASA7,GASA8,GASA9,GASA10,GASA11 and GASA12 were used as reporters and it was found that all GASA genes expressed in the growing and differentiating organs and abscission zones,suggesting the role of these genes in cell growth and *** study provided an important basis for functional study of the GASA gene family in the GA and ABA signaling pathway.

关键词： GASA DELLA GA ABA 基因

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