Morphological and molecular characterization of the rice root-knot nematode, Meloidogyne graminicola, Golden and Birchfeild, 1965 occurring in Zhejiang, China

作     者：TIAN Zhong-ling Munawar Maria Eda Marie Barsalote Pablo Castillo ZHENG Jing-wu 

作者机构：Laboratory of Plant Nematology Institute of Biotechnology College of Agriculture and Biotechnology Zhejiang University Institute for Sustainable Agriculture(IAS) Spanish National Research Council (CSIC) Campus de Excelencia Internacional Agroalimentario Key Lab of Molecular Biology of Crop Pathogens and Insects Ministry of Agriculture 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2018年第17卷第12期

页      面：2724-2733页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：supported by the Special Fund for Agro-scientific Research in the Public Interest in China(201503114) 

主　　题：Meloidogyne graminicola morphology morphometric molecular rice rDNA cytochrome c oxidase subunit Ⅱ (coxⅡ) China 

摘      要：The rice root-knot nematode Meloidogyne graminicola is a severe pest of rice. In China, it was first reported from Hainan Province, and later from several other provinces. In the present study, a rice root-knot nematode population found from the rice cultivation areas of Zhejiang Province, China is characterized via molecular analysis using internal transcribed spacer(ITS) and cytochrome c oxidase subunit Ⅱ(coxⅡ)-16 S rRNA genes and scanning electron microscopy(SEM) observations of males and the second-stage juveniles. Morphometric data and molecular sequence comparisons for all M. graminicola populations occurring in China are also provided. The overall morphology of M. graminicola found in Zhejiang match well with the original description, though males have a slightly longer body and stylet, and a shorter tail, while the second-stage juvenile is also slightly longer than in the original description. This is the first report of M. graminicola from Zhejiang. Phylogenetic studies based on coxⅡ suggest that all the Chinese populations belong to Type B. This study expands knowledge of the increasing distribution and phylogenetic relationships of M. graminicola that occur in China.