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Anti-infective immune functions of type IV interferon in grass carp(Ctenopharyngodon idella):A novel antibacterial and antiviral interferon in lower vertebrates

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Zoological Research 2024年第5期45卷 972-982页

作者： Yuchen Liu Wentao Zhu Yanqi Zhang Jingjing Zhang Maolin Lv Jianguo Su Hubei Hongshan laboratory College of FisheriesHuazhong Agricultural UniversityWuhanHubei 430070China laboratory for marine biology and biotechnology Qingdao Marine Science and Technology CenterQingdaoShandong 266237China

Type IV interferon(IFN-υ)is a recently discovered cytokine crucial for host defense against viral ***,the role and mechanisms of IFN-υin bacterial infections remain *** study investigated the antibacterial and antiviral functions and mechanisms of grass carp(Ctenopharyngodon idella)IFN-υ(CiIFN-υ)both in vivo and in *** CiIFN-υgene was first identified and characterized in grass ***,the immune expression of CiIFN-υsignificantly increased following bacterial challenge,indicating its response to bacterial *** eukaryotic recombinant expression plasmid of CiIFN-υwas then constructed and transfected into fathead minnow(FHM)*** were collected and incubated with four bacterial strains,followed by plate spreading and colony *** indicated that CiIFN-υexhibited more potent antibacterial activity against gram-negative bacteria compared to gram-positive bacteria and aggregated gram-negative bacteria but not gram-positive *** vivo experiments further confirmed the antibacterial function,showing high survival rates,low tissue edema and damage,reduced tissue bacterial load,and elevated proinflammatory response at the early stages of bacterial *** addition,the antiviral function of CiIFN-υwas confirmed through in vitro and in vivo experiments,including crystal violet staining,survival rates,tissue viral burden,and reverse transcription-quantitative real-time polymerase chain reaction(RT-qPCR).This study highlights the antibacterial function and preliminary mechanism of IFN-υ,demonstrating that IFN-υpossesses dual functions against bacterial and viral infections.

关键词： Grass carp(Ctenopharyngodon idella) IFN-υ Bactericidal activity Antiviral activity Antimicrobial immunity

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Pufferfish gasdermin Ea is a significant player in the defense against bacterial pathogens

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marine Life Science & Technology 2024年第3期6卷 462-474页

作者： Hang Xu Kunpeng Qin Kangwei Hao Zihao Yuan Li Sun CAS and Shandong Province Key laboratory of Experimental marine biology Institute of OceanologyCAS Center for Ocean Mega-ScienceChinese Academy of SciencesQingdao 266071China laboratory for marine biology and biotechnology Laoshan LaboratoryQingdao 266237China School of marine Science University of Chinese Academy of SciencesQingdao 266400China

Gasdermins(GSDMs)are proteins cleaved by caspase(CASP)to trigger *** teleosts,pyroptosis is mediated by gasdermin E(GSDME).The Pufferfish,Takifugu rubripes,possesses two GSDME orthologs:named TrGSDMEa and *** is cleaved by CASP3/7 to liberate the N-terminal(NT)domain that can trigger pyroptosis in mammalian ***,the biological function of TrGSDMEa in pufferfish is unknown,and TrGSDMEb is poorly *** found that TrGSDMEb was cleaved by CASP1/3/6/7/8,but the resulting NT domain,despite its similarity to TrGSDMEa-NT domain in sequence and structure,failed to induce *** and TrGSDMEb exhibited similar expression patterns in pufferfish under normal physiological conditions but were up-and downregulated,respectively,in expression during Vibrio harveyi and Edwardsiella tarda *** infection induced the activation of TrGSDMEa and CASP3/7 in pufferfish cells,resulting in pyroptosis accompanied with IL-1βproduction and *** of TrGSDMEa-mediated pyroptosis via TrCASP3/7 reduced the death of pufferfish cells and augmented bacterial dissemination in fish ***-oriented mutagenesis identified 16 conserved residues in teleost GSDMEa that were required for the pore formation or auto-inhibition of *** study illustrates the role of GSDMEa-mediated pyroptosis in teleost defense against bacterial pathogens and provides new insights into the structure-based function of vertebrate GSDME.

关键词： GSDME Caspase Takifugu rubripes Bacterial infection Immune defense

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Full T-cell activation and function in teleosts require collaboration of first and co-stimulatory signals

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Zoological Research 2024年第1期45卷 13-24页

作者： Wei Liang Kang Li Haiyou Gao Kunming Li Jiansong Zhang Qian Zhang Xinying Jiao Jialong Yang Xiumei Wei State Key laboratory of Estuarine and Coastal Research School of Life SciencesEast China Normal UniversityShanghai 200241China laboratory for marine biology and biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdaoShandong 266237China

Mammalian T-cell responses require synergism between the first signal and co-stimulatory ***,whether and how dual signaling regulates the T-cell response in early vertebrates remains *** the present study,we discovered that the Nile tilapia(Oreochromis niloticus)encodes key components of the LAT signalosome,namely,LAT,ITK,GRB2,VAV1,SLP-76,GADS,and PLC-γ*** components are evolutionarily conserved,and CD3εmAb-induced T-cell activation markedly increased their ***,at least ITK,GRB2,and VAV1 were found to interact with LAT for signalosome *** of the first signal,the NF-κB,MAPK/ERK,and PI3K-AKT pathways were activated upon CD3εmAb ***,treatment of lymphocytes with CD28 mAbs triggered the AKT-mTORC1 pathway downstream of the co-stimulatory *** CD3εand CD28 mAb stimulation enhanced ERK1/2 and S6 phosphorylation and elevated NFAT1,c-Fos,IL-2,CD122,and CD44 expression,thereby signifying T-cell ***,rather than relying on the first or co-stimulatory signal alone,both signals were required for T-cell *** T-cell activation was accompanied by marked apoptosis and cytotoxic *** findings suggest that tilapia relies on dual signaling to maintain an optimal T-cell response,providing a novel perspective for understanding the evolution of the adaptive immune system.

关键词： Oreochromis niloticus CD3 CD28 T cells Adaptive immunity Evolution

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Characterization,expression dynamics,and potential function of OPA1 for regulation of mitochondrial morphology during spermiogenesis in Phascolosoma esculenta

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Journal of Oceanology and Limnology 2024年第1期42卷 187-200页

作者： Xinming GAO Binbin FENG Chen DU Congcong HOU Shan JIN Daojun TANG Junquan ZHU Key laboratory of Applied marine biotechnology by the Ministry of Education and Key laboratory of marine biotechnology of Zhejiang Province School of Marine SciencesNingbo UniversityNingbo 315211China College of Ecology Lishui UniversityLishui 323000China

Mitochondria undergo morphological changes during spermatogenesis in some *** mechanism and role of mitochondrial morphology regulation,however,remain somewhat *** this study,we analyzed the molecular characteristics,expression dynamics and subcellular localization of optic atrophy protein 1(OPA1),a mitochondrial fusion and cristae maintenance-related protein,to reveal the possible regulatory mechanisms underlying mitochondrial morphology in Phascolosoma esculenta *** full-length cDNA of the *** opa1 gene(Pe-opa1)is 3743 bp in length and encodes 975 amino *** Pe-OPA1 protein is highly conservative and includes a transmembrane domain,a GTPase domain,two helical bundle domains,and a lipid-interacting *** and protein expression was higher in the coelomic fluid(a site of spermatid development)of male *** and increased first and then decreased from March to ***,their expression during the breeding stage was significantly higher than during the non-breeding stage,suggesting that Pe-OPA1 is involved in *** *** Pe-OPA1 protein was more abundant in components consisting of many spermatids than in components without,indicating that Pe-OPA1 mainly plays a role in the spermatid in coelomic ***,Pe-OPA1 was mainly detected in the spermatid *** experiments showed that the Pe-OPA1 are constitutively expressed and co-localized with mitochondria during spermiogenesis,suggesting its involvement in *** *** results provide evidence for Pe-OPA1's involvement in the regulation of mitochondrial morphology during spermiogenesis.

关键词： optic atrophy protein 1(OPA1) spermiogenesis Phascolosoma esculenta mitochondrial dynamics spermatid

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Photoperiod Mediates the Effects of Temperature and Light Intensity on the Proliferation of Ulva prolifera

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Journal of Ocean University of China 2024年第1期23卷 255-263页

作者： JIANG Jianan YU Yanyan CHEN Yili LI Yahe XU Nianjun Key laboratory of marine biotechnology of Zhejiang Province School of Marine SciencesNingbo UniversityNingbo 315211China

In order to study the complex effects of photoperiod,temperature,and light intensity on the spore maturation and release number of Ulva prolifera,we cultured thalli segment(2–3 mm)under three different photoperiods(L:D=12:12,14:10 and 10:14),temperature(15℃(LT),25℃(MT)and 30℃(HT))and light intensity(100,200 and 400μmol m^(−2)s^(−1),noted as LL,ML and HL,respectively)*** the maturation time,spore release number and chlorophyll fluorescence were *** results suggested that:1)The spore maturation time was accelerated by higher temperature or higher light intensity from 62 h to 36 h,and changes in day length accelerated the spore maturation to a certain extent as compared with 12:12 light/dark cycle;2)Higher light intensity significantly decreased the chlorophyll fluorescence(Fv′/Fm′,NPQ,rETRmax andα)of the mature reproductive segment under 30℃with 12:12 light/dark *** when in the other photoperiods(10:14 and 14:10 conditions),the inhibitory effects of high light intensity were alleviated significantly;3)The optimum condition for the spore maturation and release was 12:12 light/dark cycle,25℃,400μmol m^(−2)s^(−1),with both shorter and longer photoperiod reducing the spore release number;4)Higher light intensity significantly increased the spore release number under 25℃,but these effects were alleviated by 30℃*** study is the first attempt to elucidate the coincidence effects of photoperiod,temperature and light intensity on the reproduction of Ulva,which would help to reveal the mechanism of the rapid proliferation of green tide.

关键词： light intensity maturation photoperiod reproduction temperature Ulva prolifera

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Anti-infective immune functions of type Ⅳ interferon in grass carp(Ctenopharyngodon idella): A novel antibacterial and antiviral interferon in lower vertebrates

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Zoological Research 2024年第05期 972-982页

作者： Yuchen Liu Wentao Zhu Yanqi Zhang Jingjing Zhang Maolin Lv Jianguo Su Hubei Hongshan laboratory College of Fisheries Huazhong Agricultural University laboratory for marine biology and biotechnology Qingdao Marine Science and Technology Center

Type Ⅳ interferon(IFN-υ) is a recently discovered cytokine crucial for host defense against viral infections. However,the role and mechanisms of IFN-υ in bacterial infections remain unexplored. This study investigated the antibacterial and antiviral functions and mechanisms of grass carp(Ctenopharyngodon idella) IFN-υ(CiIFN-υ) both in vivo and in vitro. The CiIFN-υ gene was first identified and characterized in grass carp. Subsequently, the immune expression of CiIFN-υ significantly increased following bacterial challenge, indicating its response to bacterial infections. The eukaryotic recombinant expression plasmid of CiIFN-υ was then constructed and transfected into fathead minnow(FHM) cells. Supernatants were collected and incubated with four bacterial strains,followed by plate spreading and colony counting. Results indicated that CiIFN-υ exhibited more potent antibacterial activity against gram-negative bacteria compared to grampositive bacteria and aggregated gram-negative bacteria but not gram-positive bacteria. In vivo experiments further confirmed the antibacterial function, showing high survival rates, low tissue edema and damage, reduced tissue bacterial load, and elevated proinflammatory response at the early stages of bacterial infection. In addition, the antiviral function of CiIFN-υ was confirmed through in vitro and in vivo experiments, including crystal violet staining,survival rates, tissue viral burden, and reverse transcription-quantitative real-time polymerase chain reaction(RT-qPCR). This study highlights the antibacterial function and preliminary mechanism of IFN-υ,demonstrating that IFN-υ possesses dual functions against bacterial and viral infections.

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OsATL32 ubiquitinates the reactive oxygen species-producing OsRac5-OsRbohB module to suppress rice immunity

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Journal of Integrative Plant biology 2024年第7期66卷 1459-1480页

作者： Yuqing Yan Hui Wang Yan Bi Jiajing Wang Muhammad Noman Dayong Li Fengming Song National Key laboratory for Rice biology and Breeding Institute of BiotechnologyZhejiang UniversityHangzhou 310058China Ministry of Agriculture Key laboratory of Molecular biology of Crop Pathogens and Insect Pests Institute of BiotechnologyZhejiang UniversityHangzhou 310058China Key laboratory of biology of Crop Pathogens and Insects of Zhejiang Province Institute of BiotechnologyZhejiang UniversityHangzhou 310058China

Ubiquitination-mediated protein degradation is integral to plant immunity,with E3 ubiquitin ligases acting as key factors in this ***,we report the functions of OsATL32,a plasma membrane-localized Arabidopsis Tóxicos En Levadura(ATL)-type E3 ubiquitin ligase,in rice(Oryza sativa)immunity and its associated regulatory *** found that the expression of OsATL32 is downregulated in both compatible and incompatible interactions between rice and the rice blast fungus Magnaporthe *** OsATL32 protein level declines in response to infection by a compatible *** strain or to chitin ***32 negatively regulates rice resistance to blast and bacterial leaf blight diseases,as well as chitin-triggered *** and genetic studies revealed that OsATL32 suppresses pathogen-induced reactive oxygen species(ROS)accumulation by mediating ubiquitination and degradation of the ROS-producing OsRac5–OsRbohB module,which enhances rice immunity against *** protein phosphatase PHOSPHATASE AND TENSIN HOMOLOG enhances rice blast resistance by dephosphorylating OsATL32 and promoting its degradation,preventing its negative effect on rice *** study provides insights into the molecular mechanism by which the E3 ligase OsATL32 targets a ROS-producing module to undermine rice immunity.

关键词： E3 ligase OsATL32 OsRac5-OsRbohB module phosphatase OsPTEN reactive oxygen species rice immunity

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Combined proteomic and metabolomic studies on the liver of Amur sturgeon Acipenser schrenckii under titanium dioxide nanoparticle exposure

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Journal of Oceanology and Limnology 2024年第3期42卷 1001-1015页

作者： Zhou ZHOU Ting LIU Jie KONG Zhenxing ZHAO Junquan ZHU Key laboratory of Aquacultural biotechnology Ministry of Educationand Key Laboratory of Marine Biotechnology of Zhejiang ProvinceCollege of Marine SciencesNingbo UniversityNingbo 315000China Guizhou Fisheries Research Institute Guizhou Academy of Agriculture SciencesGuiyang 550000China

Nanomaterials,particularly titanium dioxide nanoparticles(TiO_(2)-NPs),are extensively utilized across various ***,their environmental release has raised concerns regarding their potential ecological and environmental *** reproductive toxicity of TiO_(2)-NPs in fish species has attracted considerable attention,yet conflicting research outcomes have been *** investigated the effects of TiO_(2)-NPs exposure on the liver of juvenile Amur sturgeon Acipenser schrenckii using label-free proteomic and untargeted metabolomic *** experiment included a control group and three groups exposed to different concentrations of TiO_(2)-NPs(low,TL;medium,TM;high,TH).Compared to the control group,9,19,and 25 proteins and 35,73,and 158 metabolites were differentially expressed in the TH,TM,and TL TiO_(2)-NP-exposed groups,*** differentially expressed genes(DEGs)were enriched in the Kyoto Encyclopedia for Genes and Genomes(KEGG)pathways related to glycolysis and ***,among the 126 correlated proteins,the most enriched pathways were associated with endocytosis and protein processing in the endoplasmic ***,syringic acid was significantly downregulated across all three TiO_(2)-NP-exposed *** obtain a comprehensive overview of the TiO_(2)-NP-induced expression changes,a co-regulated network of proteins and metabolites associated with TiO_(2)-NPs exposure was *** to TiO_(2)-NPs led to enrichment and alteration of pathways related to immune responses,including endocytosis,protein processing in the endoplasmic reticulum,and peroxisome proliferator-activated receptor(PPAR)*** conclusion,our findings indicate that exposure to TiO_(2)-NPs might disrupt glucose metabolism and induce immune responses,thus contributing to our understanding of the environmental impacts of nanomaterials and highlighting the need for further research and development of potential mitigation strategies.

关键词： TiO_(2)-nanoparticle exposure proteomics metabolomics glucose metabolism immune response

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Taxonomic dependency and spatial heterogeneity in assembly mechanisms of bacteria across complex coastal waters

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Ecological Processes 2024年第1期13卷 80-97页

作者： Huizhen Yan Dandan Lin Gaoke Gu Yujie Huang Xuya Hu Zhenhao Yu Dandi Hou Demin Zhang Barbara J.Campbell Kai Wang State Key laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products School of Marine SciencesNingbo University Key laboratory of marine biotechnology of Zhejiang Province School of Marine Sciences Ningbo University Department of Biological Sciences Clemson University

Background Understanding community assembly mechanisms across taxa and space is fundamental for microbial ecology. However, the variability and determinants of assembly processes over taxa and space remain unclear. Here, we investigated taxonomic dependency and spatial heterogeneity in bacterial assembly mechanisms across coastal waters in the East China Sea using neutral and null models with customized visualization *** Overall, bacterial assembly mechanisms varied across broad taxonomic groups(phyla and proteobacterial classes) and space at the regional scale. A determinism–stochasticity balanced mechanism governed total bacterial assembly, while taxonomic dependency existed in assembly mechanisms and ecological processes. Among community ecological features, niche breadth and negative-to-positive cohesion ratio were strongly associated with the determinism-to-stochasticity ratio of bacterial groups. Bacterial assembly mechanisms commonly exhibited spatial heterogeneity, the extent and determinants of which varied across taxonomic groups. Spatial assembly of total bacteria was directly driven by many environmental factors and potential interactions between taxa, but not directly by geographic factors. Overall, the bacterial groups with higher spatial heterogeneity in assembly mechanisms were more related to environmental and/or geographic factors(except Bacteroidetes), while those with lower heterogeneity were more related to ecological *** Our results confirm the pervasiveness of taxonomic dependency and spatial heterogeneity in bacterial assembly, providing a finer understanding about regulation across complex coastal waters.

关键词： Bacterioplankton Assembly mechanism Niche breadth Null model Environmental gradient Biogeography

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Effect of dietary supplement of inactivated Lactobacillus plantarum Ep-M 17 on growth performance,immune response,disease resistance,and intestinal microbiota in Penaeus vannamei

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Journal of Oceanology and Limnology 2024年第2期42卷 676-694页

作者： Ting WU Xiaoman HU Wenlong XU Yang DU Jiong CHEN State Key laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Ningbo UniversityNingbo 315211China laboratory of Biochemistry and Molecular biology School of Marine SciencesNingbo UniversityNingbo 315211China Key laboratory of Applied marine biotechnology of Ministry of Education Ningbo UniversityNingbo 315211China

Our previous study found that feeding with Lactobacillus plantarum Ep-M17 could effectively affect the growth performance,immune response,and gut microbiota of Penaeus ***,high temperature and pressure during feed pelletizing is the main problem that can lead to a decrease in the activity of probiotics or cause their *** investigation needs to investigate whether inactivated Ep-M17 can exert similar effects as live ***,we evaluated the effects of inactivated *** Ep-M17 on growth performance,immune response,disease resistance,and gut microbiota in *** show that adding inactivated Ep-M17 to the feed also promoted body weight gain and increased relative immune protection in ***,histological examination revealed that the administration of inactivated Ep-M17 led to improvements in the density and distribution of microvilli in the intestines and enhancements in the abundance of B and R cells in the ***,the inactivated Ep-M17 supplementation resulted in increased activity levels of nutrient immune-related enzymes in both the shrimp hepatopancreas and ***,it stimulated the expression of Lvlec,PEN-3a,Crustin,LGBP,Lysozyme,and proPo genes in both the hepatopancreas and ***,the inactivated Ep-M17 also increased bacterial diversity in the gut of shrimp and promoted the abundance of specific flora,facilitating the host organism’s metabolism and immunity to improve the disease resistance of ***,supplementation of inactivated *** Ep-M17 in shrimp diets can exert similar effects as live *** Ep-M17 effectively improving growth performance,gut microbiota,immune response,and disease resistance in ***.

关键词： Penaeus vannamei Lactobacillus plantarum Ep-M17 inactivated probiotics immune response gut flora

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