Full T-cell activation and function in teleosts require collaboration of first and co-stimulatory signals
作者机构:State Key Laboratory of Estuarine and Coastal ResearchSchool of Life SciencesEast China Normal UniversityShanghai 200241China Laboratory for Marine Biology and BiotechnologyQingdao National Laboratory for Marine Science and TechnologyQingdaoShandong 266237China
出 版 物:《Zoological Research》 (动物学研究(英文))
年 卷 期:2024年第45卷第1期
页 面:13-24页
核心收录:
学科分类:0710[理学-生物学] 07[理学] 071009[理学-细胞生物学]
基 金:supported by the National Key Research and Development Program(2022YFD2400804) National Natural Science Foundation of China(32022086,31972822) Natural Science Foundation of Shanghai(20ZR1417500)
主 题:Oreochromis niloticus CD3 CD28 T cells Adaptive immunity Evolution
摘 要:Mammalian T-cell responses require synergism between the first signal and co-stimulatory ***,whether and how dual signaling regulates the T-cell response in early vertebrates remains *** the present study,we discovered that the Nile tilapia(Oreochromis niloticus)encodes key components of the LAT signalosome,namely,LAT,ITK,GRB2,VAV1,SLP-76,GADS,and PLC-γ*** components are evolutionarily conserved,and CD3εmAb-induced T-cell activation markedly increased their ***,at least ITK,GRB2,and VAV1 were found to interact with LAT for signalosome *** of the first signal,the NF-κB,MAPK/ERK,and PI3K-AKT pathways were activated upon CD3εmAb ***,treatment of lymphocytes with CD28 mAbs triggered the AKT-mTORC1 pathway downstream of the co-stimulatory *** CD3εand CD28 mAb stimulation enhanced ERK1/2 and S6 phosphorylation and elevated NFAT1,c-Fos,IL-2,CD122,and CD44 expression,thereby signifying T-cell ***,rather than relying on the first or co-stimulatory signal alone,both signals were required for T-cell *** T-cell activation was accompanied by marked apoptosis and cytotoxic *** findings suggest that tilapia relies on dual signaling to maintain an optimal T-cell response,providing a novel perspective for understanding the evolution of the adaptive immune system.