Full T-cell activation and function in teleosts require collaboration of first and co-stimulatory signals

作     者：Wei Liang Kang Li Haiyou Gao Kunming Li Jiansong Zhang Qian Zhang Xinying Jiao Jialong Yang Xiumei Wei 

作者机构：State Key Laboratory of Estuarine and Coastal ResearchSchool of Life SciencesEast China Normal UniversityShanghai 200241China Laboratory for Marine Biology and BiotechnologyQingdao National Laboratory for Marine Science and TechnologyQingdaoShandong 266237China 

出 版 物：《Zoological Research》 (动物学研究（英文）)

年 卷 期：2024年第45卷第1期

页      面：13-24页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 

基　　金：supported by the National Key Research and Development Program(2022YFD2400804) National Natural Science Foundation of China(32022086,31972822) Natural Science Foundation of Shanghai(20ZR1417500) 

主　　题：Oreochromis niloticus CD3 CD28 T cells Adaptive immunity Evolution 

摘      要：Mammalian T-cell responses require synergism between the first signal and co-stimulatory ***,whether and how dual signaling regulates the T-cell response in early vertebrates remains *** the present study,we discovered that the Nile tilapia(Oreochromis niloticus)encodes key components of the LAT signalosome,namely,LAT,ITK,GRB2,VAV1,SLP-76,GADS,and PLC-γ*** components are evolutionarily conserved,and CD3εmAb-induced T-cell activation markedly increased their ***,at least ITK,GRB2,and VAV1 were found to interact with LAT for signalosome *** of the first signal,the NF-κB,MAPK/ERK,and PI3K-AKT pathways were activated upon CD3εmAb ***,treatment of lymphocytes with CD28 mAbs triggered the AKT-mTORC1 pathway downstream of the co-stimulatory *** CD3εand CD28 mAb stimulation enhanced ERK1/2 and S6 phosphorylation and elevated NFAT1,c-Fos,IL-2,CD122,and CD44 expression,thereby signifying T-cell ***,rather than relying on the first or co-stimulatory signal alone,both signals were required for T-cell *** T-cell activation was accompanied by marked apoptosis and cytotoxic *** findings suggest that tilapia relies on dual signaling to maintain an optimal T-cell response,providing a novel perspective for understanding the evolution of the adaptive immune system.