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Immunity of the greater wax moth Galleria mellonella

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Insect Science 2017年第3期24卷 342-357页

作者： Iwona Wojda maria curie-sklodowska university Faculty of Biology and Biotechnology Institute of Biology and Biochemistry Department of Immuno-biology Akademicka 19 20-033 Lublin Poland

Investigation of insect immune mechanisms provides important information concerning innate immunity, which in many aspects is conserved in animals. This is one of the reasons why insects serve as model organisms to study virulence mechanisms of human pathogens. From the evolutionary point of view, we also learn a lot about host-pathogen interaction and adaptation of organisms to conditions of life. Additionally, insect-derived antibacterial and antifungal peptides and proteins are considered for their potential to be applied as alternatives to antibiotics. While Drosophila melanogaster is used to study the genetic aspect of insect immunity, Galleria mellonella serves as a good model for biochemical research. Given the size of the insect, it is possible to obtain easily hemolymph and other tissues as a source of many immune-relevant polypeptides. This review article summarizes our knowledge concerning G. mellonella immunity. The best-characterized immune-related proteins and peptides are recalled and their short characteristic is given. Some other proteins identified at the mRNA level are also mentioned. The infectious routes used by Galleria natural pathogens such as Bacillus thuringiensis and Beauveria bassiana are also described in the context of host-pathogen interaction. Finally, the plasticity of G. mellonella immune response influenced by abiotic and biotic factors is described.

关键词： Bacillus thuringiensis Beauveria bassiana defense proteins and peptides Galleria mellonella insect immunity

Influence of intralipid on free propofol fraction assayed in human serum albumin solutions and human plasma

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Acta Pharmacologica Sinica 2006年第12期27卷 1637-1641页

作者： Rafal KALITYNSKI Andrzej LDAWIDOWICZ Jacek POSZYTEK Department of Chromatographic Methods Maria Curie-Sklodowska UniversityLublin PL 20031Poland

Aim:It is generally assumed that only unbound drugs can reach the site of actionby diffusing across the membranes and exerting pharmacological effects by inter-acting with *** research has shown that the percentage of freedrugs may depend on the total drug *** aim of the paper is toverify whether the mentioned dependence reported for propofol also takes placein plasma and human serum albumin samples in the presence of intralipid-themedium used as a vehicle for propofol infusions and a parenteral nutrition ***:Artificial plasma samples and human plasma were spiked with intralipidor ethanolic solutions of *** samples were then assayed for free propofolconcentration using ultrafiltration and high performance liquid chromatographywith fluorimetric ***:The decrease of the total drug concentrationresults in free propofol fraction increase,irrespectively of the used type of propofolsolvent and sample *** addition of intralipid causes the lowering of theoverall free drug fraction with respect to the samples spiked with ethanolic solu-tions of the ***:The presence of intralipid does not influence thephenomenon of free propofol fraction rise at low total drug *** arise cannot be ignored in clinical conditions when the drug is applied for sedative,antiemetic or other low-dosage purposes.

关键词： propofol unbound drugs human serum albumin drug binding intralipid plasma

Potential energy surfaces and fission fragment mass yields of even-even superheavy nuclei

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Chinese Physics C 2021年第12期45卷 155-174页

作者： Pavel V.Kostryukov Artur Dobrowolski Bozena Nerlo-Pomorska Michal Warda Zhigang Xiao Yongjing Chen Lile Liu Jun-Long Tian Krzysztof Pomorski Institute of Physics Maria Curie Sklodowska University20-031 LublinPoland Department of Physics Tsinghua UniversityBeijing 100084China Institute of Atomic Energy Beijing 102413China School of Physics and Electrical Engineering Anyang Normal UniversityAnyang 455000China

Potential energy surfaces and fission barriers of superheavy nuclei are analyzed in a macroscopic-microscopic *** Lublin-Strasbourg Drop(LSD)model is used to obtain the macroscopic part of the energy,whereas the shell and pairing energy corrections are evaluated using the Yukawa-folded potential;a standard flooding technique is utilized to determine barrier heights.A Fourier shape parametrization containing only three deformation parameters is shown to effectively reproduce the nuclear shapes of nuclei approaching *** addition,a non-axial degree of freedom is taken into account to better describe the structure of nuclei around the ground state and in the saddle *** addition to the symmetric fission valley,a new highly asymmetric fission mode is predicted in most superheavy *** fission fragment mass distributions of the considered nuclei are obtained by solving 3D Langevin equations.

关键词： nuclear fission mac-mic model fission barrier heights fragment mass yields

Sorption of lanthanide ions on biochar composites

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Journal of Rare Earths 2018年第11期36卷 1212-1220页

作者： Dorota Kotodyniska Justyna Bak Malwina Majdariska Dominika Fila Department of Inorganic Chemistry Faculty of ChemistryMaria Curie-Sktodowska UniversityM.Curie Sktodowska 5q.220-031 tublinPoland

Sorption of lanthanum（Ⅲ）, cerium（Ⅲ and neodymium（Ⅲ） ions from the aqueous solutions of mixtures through adsorption on the biochar composites was investigated as a function of sorbent mass, pH, phase contact time and initial concentration of solutions at 295 K. The maximum removal of lanthanide ions takes place under the following conditions： 0.1 g of sorbent mass, pH 4 and 360 rain contact time for all studied initial concentrations of solutions. Kinetics of La（Ⅲ）, Ce（Ⅲ） and Nd（Ⅲ） ions sorption proceeded by a fast initial uptake reached equilibrium. This process was modelled by means of the pseudo first order, pseudo second order, intraparticle diffusion and Elovich models. The desorption of three lanthahide ions by nitric, hydrochloric and sulfuric acids at a concentration of 1 mol/L from biochar composites was also studied. In order to investigate the sorption mechanism FFIR, XRD and XPS analyses were performed after sorption of ions from the mixture.

关键词： Lanthanide ions Biochar composites Sorption mechanism Zerovalent iron Rare earths

Fission fragment mass yields of Th to Rf even-even nuclei

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Chinese Physics C 2021年第5期45卷 179-189页

作者： Krzysztof Pomorski Jose M.Blanco Pavel V.Kostryukov Artur Dobrowolski Bozena Nerlo-Pomorska Michal Warda Zhi-Gang Xiao Yong-Jing Chen Li-Le Liu Jun-Long Tian Xin-Yue Diao Qiang-Hua Wu Institute of Physics Maria Curie Sklodowska University20-03 I LublinPoland Department of Physics Tsinghua UniversityBeijing 100084China China Institute of Atomic Energy Bejjing 102413China School of Physics and Electrical Engineering Anyang Normal UniversityAnyang 455000China

Fission properties of the actinide nuclei are deduced from theoretical *** investigate potential energy surfaces and fission barriers and predict the fission fragment mass yields of actinide *** results are compared with experimental data where *** calculations were performed in the macroscopic-microscopic approximation With the Lublin-Strasbourg Drop(LSD)for the macroscopic part,and the microscopic energy corrections were evaluated in the Yukawa-folded *** Fourier nuclear shape parametrization is used to describe the nuclear shape,including the non-axial degree of *** fission fragment mass yields of the nuclei considered are evaluated within a 3D colletive model using the Borm-Oppenheimer approximation.

关键词： nuclear fission mac-mic model fission barrier heights fragment mass yields

Trace elements and stable sulfur isotopes in plants of acid mine drainage area:Implications for revegetation of degraded land

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Journal of Environmental Sciences 2020年第8期32卷 128-136页

作者： Agnieszka Gałuszka Zdzisław MMigaszewski Andrzej Pelc Andrzej Trembaczowski Sabina Doł˛egowska Artur Michalik Geochemistry and the Environment Division Institute of ChemistryJan Kochanowski University7 Uniwersytecka St.25-406 KielcePoland Mass Spectrometry Laboratory Institute of PhysicsMaria Curie-Skłodowska University1 Maria Curie-Skłodowska Sq.20-031 LublinPoland

The abundances of trace elements,a low pH of water and soil in areas impacted by the acid mine drainage(AMD)may cause an excessive uptake of potentially toxic elements and nutritional imbalances in ***-tolerant,native plants are used for revegetation of degraded mining *** established levels of selected trace elements and stable sulfur isotopes in the above-ground plant biomass collected in a mining area in south-central *** 2016,20 samples of the most common species were collected from sites with a different influence of acid mine drainage and analyzed for trace elements by the inductively coupled plasma mass spectrometry *** the basis of the results obtained in 2016,the most contaminated site was selected for a more detailed study,in which sulfur contents and stable sulfur isotope ratios were determined together with trace elements in 17 *** results confirmed that the plants native to the AMD area efficiently accumulated trace elements,especially As and rare earth *** showed the highest content of trace elements,but exhibited the lowest concentrations of sulfur accompanied by the highestδ34S *** has been shown for the first time that stable sulfur isotope composition of AMD plants in south-central Poland is significantly depleted in the 34S isotope showing an averageδ34 S value of–10.5‰in comparison with positiveδ34S values in local vegetation growing outside the AMD area and in local precipitation.

关键词： Trace elements Stable sulfur isotopes Pyrite oxidation Rare earth elements Plants

Novel synthesis method combining a foaming agent with freeze-drying to obtain hybrid highly macroporous bone scaffolds

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Journal of Materials Science & Technology 2020年第8期36卷 52-63页

作者： Paulina Kazimierczak Aleksandra Benko Krzysztof Palka Cristina Canal Dorota Kolodynska Agata Przekora Department of Biochemistry and Biotechnology Medical University of LublinChodzki 120-093LublinPoland AGH university of Science and Technology Faculty of Materials Science and CeramicsA.Mickiewicza 30 Ave.30-059KrakowPoland Department of Materials Engineering Lublin University of TechnologyNadbystrzycka 3620-618LublinPoland Biomaterials Biomechanics and Tissue Engineering GroupDepartment of Materials Science and MetallurgyUniversitat Politècnica de CatalunyaBarcelona08019Spain Research Centre in Multiscale Science and Engineering Universitat Politècnica de CatalunyaBarcelona08019Spain Department of Inorganic Chemistry Maria Curie-Sklodowska UniversityMaria Curie Sklodowska Sq.220-031LublinPoland

Three-dimensional macroporous scaffolds are commonly used in bone tissue engineering applications since they provide sufficient space for cell migration and proliferation, facilitating bone ingrowth and implant vascularisation. The aim of this work was to combine two simple methods, freeze-drying and gas-foaming, in order to fabricate highly macroporous bone scaffolds made of chitosan/agarose matrix reinforced with nanohydroxyapatite. The secondary goal of this research was to comprehensively assess biomedical potential of developed biomaterials. In this work, it was demonstrated that simultaneous application of freeze-drying and gas-foaming technique allows to obtain hybrid(as proven by ATR-FTIR)macroporous bone scaffolds(pore diameter > 50 um) characterized by high open(70%) and interconnected porosity. Novel scaffolds were non-toxic, favoured osteoblasts adhesion and growth and induced apatite formation on their surfaces, indicating their high bioactivity that is essential for good implant osseointegration. Biomaterials were also prone to enzymatic degradation, degradation in acidified microenvironment(e.g. osteoclast-mediated), and slow degradation under physiological p H of ***, the scaffolds revealed microstructure(70% open porosity, SSA approx. 30 m2/g, high share of macropores with diameter in the range 100-410 um) and compressive strength(1–1.4 MPa) comparable to cancellous bone, indicating that they are promising implants for cancellous bone regeneration.

关键词： Agarose Chitosan Nanohydroxyapatite Biomaterial porosity Biodegradation Cryogel

The study of the interactions between graphene and Ge（001）/Si（001）

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Nano Research 2017年第11期10卷 3648-3661页

作者： Pawel Dabrowski Maciej Rogala Iwona Pasternak Jacek Baranowski Wlodzimierz Strupinski Marek Kopciuszynski Ryszard Zdyb Mieczyslaw Jalochowski Iaroslav Lutsyk Zbigniew Klusek Department of Solid State Physics Faculty of Physics and Applied Informatics University of Lodz Pomorska 149/153 90-236 Lodz Poland Institute of Electronic Materials Technology Wolczynska 133 01-919 Warsaw Poland Institute of Physics Maria Curie-Sklodowska University pl.M.Curie-Sklodowskiej 1 20-031 Lublin Poland Department of Solid State Physics Yuriy Fedkovych Chernivtsi National University Kotsubinsky 2 58012 Chernivtsi Ukraine

The interaction between graphene and germanium surfaces was investigated using a combination of microscopic and macroscopic experimental techniques and complementary theoretical *** functional theory （DFT） calculations for different reconstructions of the Ge（001） surface showed that the interactions between graphene and the Ge（001） surface introduce additional peaks in the density of states,superimposed on the graphene valence and conduction energy *** growth of graphene induces nanofaceting of the Ge（001） surface,which exhibits well-organized hill and valley *** graphene regions covered by hills are of high quality and exhibit an almost linear dispersion relation,which indicates weak graphene-germanium *** the other hand,the graphene component occupying valley regions is significantly perturbed by the interaction with *** was also found that the stronger graphene-germanium interaction observed in the valley regions is connected with a lower local electrical *** of graphene/Ge（001）/Si（001） was performed to obtain a more uniform *** process results in a surface characterized by negligible hill and valley structures;however,the graphene properties unexpectedly deteriorated with increasing uniformity of the Ge（001） *** sum up,it was shown that the mechanism responsible for the formation of local conductivity inhomogeneities in graphene covering the Ge（001） surface is related to the different strength of graphene-germanium *** present results indicate that,in order to obtain high-quality graphene,the experimental efforts should focus on limiting the interactions between germanium and graphene,which can be achieved by adjusting the growth conditions.

关键词： chemical vapor deposition surface reconstruction scanning tunneling microscopy chemical bonding first-principles calculation

Characterization of Urban Soil with SEM-EDX

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American Journal of Analytical Chemistry 2016年第10期7卷 724-735页

作者： Reetu Sharma Khageshwar Singh Patel Lesia Lata Huber Milosh School of Studies in Chemistry Pt. Ravishankar Shukla University Raipur India Department of Soil Science and Protection Maria Curie-Sklodowska University Lublin Poland Geology and Lithosphere Protection Department Maria Sklodowska-Curie University Lublin Poland

The coal is largely used for generation of energy in the India, and their huge exploitations cause contamination of the soil. In the present work, the inorganic contamination of surface soil in the coal basin of the country, Korba is described. The concentration of elements i.e. C, O, S, Cl, Na, Mg, Al, Si, P, K, Ca, Ti, Fe, Mn and Ni in the surface soils (n = 30) was analysed by technique i.e. scanning electron microscope-energy dispersive X-ray spectroscopy (SEM-EDX), ranged from 6.6% - 36.4%, 37.8% - 54.3%, 0.07% - 0.39%, 0.03% - 0.25%, 0.10% - 0.39%, 0.12% - 0.49%, 3.7% - 10.3%, 12.2% - 24.4%, 0.11% - 0.60%, 0.68% - 3.22%, 0.8% - 2.7%, 0.25% - 0.61%, 2.3% - 6.4%, 0.08% - 0.22% and 0.04% - 0.16% with mean value (p = 0.05) of 18.8% ± 2.7%, 49.0% ± 1.5%, 0.18% ± 0.03%, 0.11% ± 0.02%, 0.23% ± 0.03%, 0.32% ± 0.03%, 6.1% ± 0.5%, 16.9% ± 0.9%, 0.27% ± 0.04%, 1.6% ± 0.2%, 1.5% ± 0.2%, 0.41% ± 0.03%, 4.3% ± 0.4%, 0.14% ± 0.02% and 0.08% ± 0.01%, respectively. The surface soil morphology, concentration variation of elements, pollution indices and contaminant sources in the soil are discussed.

关键词： Urban Soil Element Characterization Pollution Indices Sources SEM-EDX