检索结果-南通市图书馆

In vivo volumetric monitoring of revascularization of traumatized skin using extended depth-of-field photoacoustic microscopy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Optoelectronics 2020年第4期13卷 307-317页

作者： Zhongwen CHENG Haigang MA Zhiyang WANG Sihua YANG MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China Guangdong Provincial key laboratory of laser life science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China

Faster and better wound healing is a critical medical issue.Because the repair process of wounds is closely related to revascularization,accurate early assessment and postoperative monitoring are very important for establishing an optimal treatment plan.Herein,we present an extended depth-of-field photoacoustic microscopy system(E-DOF-PAM)that can achieve a constant spatial resolution and relatively uniform excitation efficiency over a long axial range.The superior performance of the system was verified by phantom and in vivo experiments.Furthermore,the system was applied to the imaging of normal and trauma sites of volunteers,and the experimental results accurately revealed the morphological differences between the normal and traumatized skin of the epidermis and dermis.These results demonstrated that the E-DOF-PAM is a powerful tool for observing and understanding the pathophysiology of cutaneous wound healing.

关键词： photoacoustic microscopy(PAM) extended depth-of-field traumatized skin

Light-responsive charge-reversal nanovector for high-efficiency in vivo CRISPR/Cas9 gene editing with controllable location and time

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2020年第9期13卷 2399-2406页

作者： Yunxia Wu Judun Zheng Qin Zeng Tao Zhang Da Xing MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science South China Normal UniversityGuangzhou 510631China Guangdong Provincial key laboratory of laser life science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China

Controllably and efficaciously localized CRISPR/Cas9 plasmids transfection plays an essential role in genetic editing associated with various key human diseases.We employed near-infrared(NIR)light-responsive CRISPR/Cas9 plasmids delivery via a charge-reversal nanovector to achieve highly efficient and site-specific gene editing.The nanovector with abundant positive charges was fabricated on the basis of an ultraviolet-sensitive conjugated polyelectrolyte coated on an upconversion nanomaterial(UCNP-UVP-P),which can convert into negative charges upon 980 nm light irradiation.Using the as-prepared nanovector,we demonstrated the plasmids could be efficiency transfected into tumor cells(~63%±4%)in a time-contolled manner,and that functional CRISPR/Cas9 proteins could be successfully expressed in a selected NIR-irradiated region.Particularly,this strategy was successfully applied to the delivery of CRISPR/Cas9 gene to tumor cells in vivo,inducing high efficiency editing of the target gene PLK-1 under photolrradiation.Therefore,this precisely controlled gene regulation strategy has the potential to serve as a new paradigm for gene engineering in complex biological systems.

关键词： light-responsive charge-reversal CRISPR/Cas9 gene editing

Photoacoustic nanoprobe forβ-galactosidase activity detection and imaging in vivo

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Innovative Optical Health sciences 2019年第3期12卷 16-23页

作者： Qin Zeng Yunxia Wu Tao Zhang MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science College of Biophotonics South China Normal University Guangzhou 510631P.R.China

Detection and visualization ofβ-galactosidase(β-gal)is essential to reffect its physiological and pathological effects on human health and disease,but it is still challenging to precisely trackβ-gal in vivo owing to the limitation of current analytical methods.In our work,we reported a photoacoustic(PA)nanoprobe for selective imaging of the endogenousβ-gal in vivo.Our nanoprobe Cy7-β-gal-LP was constructed by encapsulation of a near-infra red(NIR)dye Cy7-β-gal within a liposome(LP,DSPE-PEG2000-COOH).The dye Cy7-β-gal was synthesized based on a dye Cy-OH where the hydroxyl group was replaced by aβ-D-galactopyranoside residue,which can be recognized byβ-gal as an enzyme hydrolytic site.With the addition ofβ-gal,the absorbance of Cy7-β-gal exhibited a significant red shift with the absorption peak moved from 600 nm to 680 nm,which should generate a switch-on PA signal at 680 nm in the presence ofβ-gal.In addition,as theffuorescence of the dye was totally quenched due to aggregation within the liposome,Cy7-β-gal-LP exhibited high PA conversion efficiency.With the nanoprobe,we achieved the selective PA detection and imaging ofβ-gal in the tumor-bearing mice.

关键词： β-galactosidase photoacoustic imaging

Vacancy-defect-dipole amplifies the thermoacoustic conversion efficiency of carbon nanoprobes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2020年第9期13卷 2413-2419页

作者： Wei Fang Yujiao Shi Da Xing MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science South China Normal UniversityGuangzhou 510631China College of Biophotonics South China Normal UniversityGuangzhou 510631China

The immense potential of carbon nanoprobes(CNPs)for using as contrast agents has propelled much recent research and development in the field of thermoacoustic(TA)molecular imaging,while the proper engineering and design of such materials with required high TA conversion efficiency is still a highly challenging task.In this work,we proposed a controllable strategy to amplify the TA conversion efficiency of the CNPs by constructing vacancy defect(VD)dipoles,and systematically demonstrated the amplification mechanism through theoretical and experimental investigations.First-principles calculation results indicate that,when a carbon atom is removed from the CNPs by chemical approach,owing to local electron density redistribution,the VDs are formed at the positions of the original carbon atoms and act as the structural origin of permanent electric dipoles with the dipole moment several orders higher than that of non-defect sites.Under pulsed microwave irradiation,the VD dipoles are polarized repeatedly and significantly contribute to the conversion efficiency from absorbed electromagnetic waves to ultrasound through enhanced dielectric relaxation losses.We experimentally synthesized graphene samples with different VD densities and VD types to demonstrate the efficiency of the proposed strategy,and results coincide well with the theoretical proposition.This work offers feasible guidance to the systematic development and rational design of new high-conversion-efficiency TA CNPs via VD engineering.

关键词： thermoacoustic conversion efficiency vacancy-defect carbon nanoprobes

Aza-BODIPY-based phototheranostic nanoagent for tissue oxygen auto-adaptive photodynamic/photothermal complementary therapy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2022年第1期15卷 716-727页

作者： Meijiao Zhao Qin Zeng Xipeng Li Da Xing Tao Zhang MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science South China Normal UniversityGuangzhou 510631China Guangdong Provincial key laboratory of laser life science College of BiophotonicsSouth China Normal UniversityGuangzhou 510631China

Tumor oxygen spatial heterogeneity is a critical challenge for the photodynamic inhibition of solid tumors.Development of an intelligent nanoagent to initiate optimal therapeutics according to the localized oxygen levels is an effective settlement.Herein,we report an activatable nanoagent(BDP-Oxide nanoparticles(NPs))to enable the oxygen auto-adaptive photodynamic/photothermal complementaly treatment.Upon the nanoagent accumulated in the tumor region,the low extracellular pH could trigger the disassociation of the nanoagent to release the phototheranostic agent,BDP-Oxide,which will subsequently afford the fluorescence imaging-guided photodynamic oxidation after it gets into the outer oxygen-rich tumors.Along with the penetration deepening in the solid tumor,furthermore,BDP-Oxide could be reduced into BDP by the cytochrome P450(CYP450)enzymes activated in the low oxygen tension regions of inner hypoxic tumors,which will switch on the photothermal and photoacoustic effects.Overall,the BDP-Oxide NPs-enabled photodynamic/photothermal complementary therapy significantly suppressed the solid tumor growth(inhibition rate of 94.8%).This work proposes an intelligent platform to address the oxygen partial pressure for the optimization of cancer phototherapeutics.

关键词： boron dipyrromethene(BODIPY) complementary therapy self-adaptive spatial heterogeneity of oxygen

A smartphone-based automated fluorescence analysis system for point-of-care testing of Hg(Ⅱ)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Innovative Optical Health sciences 2022年第5期15卷 28-40页

作者： Yafei Chen Ke Zhang Yuan Liu Chunsun Zhang MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science College of Biophotonics South China Normal University Guangzhou 510631 P.R.China

This work demonstrates a smartphone-based automated fluorescence analysis system(SAFAS)for point-of-care testing(POCT)of Hg(Ⅱ).This system consists of three modules.The smartphone module is used to provide an excitation light source,and to collect and analyze fluorescent images.The dark box module is applied to integrate the desired optical elements and offers a dark environment.The cost of the integrated dark box mainly includes the upper cover,box body,lower bottom,¯xture and some optical elements which is about$109.The chip module is used for fluorescence sensing,which is composed of an upper plate,bottom plate and cloth-based chip.Due to the integration of multiple smartphone functions,the SAFAS eliminates the need for additional power sources,light sources and analysis systems.The dark box and upper and bottom plates are made by 3D printer.The cloth-based chip(about$0.005 for each chip)is fabricated using the wax screenprinting technique,with no need for expensive and complex fabrication equipments.To our knowledge,the cloth-based microfluidic fluorescence detection method combined with smartphone functions is first reported.By using optimal conditions,the designed system can realize the quantitative detection of Hg(Ⅱ),which has a linear range of 0.001–100μgmL^(-1)and a detection limit of 0.5 ngmL^(-1).Additionally,the SAFAS has been successfully applied for detecting Hg(Ⅱ)in actual water samples,with recoveries of 100.1%–111%,RSDs of 3.88%–9.74%,and fast detection time of about 1 min.Obviously,the proposed SAFAS has the advantages of high sensitivity,wide dynamic range,acceptable reproducibility,good stability and low cost.Therefore,it is believed that the presented SAFAS has great potential to perform the POCT of Hg(II)in different water samples.

关键词： Smartphone automated fluorescence detection cloth-based chip point-of-care testing Hg(II).

3D depth-coded photoacoustic microscopy with a large field of view for human skin imaging

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Optics Letters 2018年第8期16卷 58-61页

作者：程中文马海钢王志阳杨思华 moe key laboratory of laser life science & institute of laser life science South China Normal University College of Biophotonics South China Normal University

Photoacoustic （PA） microscopy comes with high potential for human skin imaging, since it allows noninvasively high-resolution imaging of the natural hemoglobin at depths of several millimeters. Here, we developed a PA microscopy to achieve high-resolution, high-contrast, and large field of view imaging of skin. A three-dimensional （3D） depth-coding technology was used to encode the depth information in PA images, which is very intuitive for identifying the depth of blood vessels in a two-dimensional image, and the vascular structure can be analyzed at different depths. Imaging results demonstrate that the 3D depth-coded PA microscopy should be translated from the bench to the bedside.

关键词： PA depth-coded photoacoustic microscopy with a large field of view for human skin imaging

Optical and fast nondestructive identification of the ages of leaded ancient pottery

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chinese Physics B 2012年第5期21卷 641-646页

作者：吴方媛杨湘波 MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science College of BiophotonicsSouth China Normal University School of Physical Education&Sports science South China Normal University

In this paper,based on the one-dimensional（1D） optical superlattice model,we calculate the average reflectivities（ARs） of leaded ancient pottery（AP） made within the last 2000 years,and find that for incident light with a suitable wavelength,the AR of the leaded AP increases monotonously with the increase in the layer number of the silvery glaze（SG） media.Based on this property,we propose an optical nondestructive method for identifying the age of leaded AP by detecting the AR.By using the exhaust algorithm and the discriminant function of variance,we obtain the optimal wavelength range of the incident light to identify the ages of the leaded AP.It is found that in the visible light band,if we choose green light with a wavelength range of 540-540.1 nm as the incident light,leaded AP made within the last 2000 years can be identified swiftly and precisely by detecting the ARs.This will be useful for designing optical instruments for the fast nondestructive identification of the ages of leaded AP.

关键词： optical superlattice ancient pottery nondestructive identification

Band rules for the frequency spectra of three kinds of aperiodic photonic crystals with negative refractive index materials

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chinese Physics B 2009年第12期18卷 5313-5325页

作者：全小林杨湘波 moe key laboratory of laser life science & institute of laser life science South China Normal University

By means of the theory of electromagnetic wave propagation and transfer matrix method, this paper investigates the band rules for the frequency spectra of three kinds of one-dimensional （1D） aperiodic photonic crystals （PCs）, generalized Fibonacci GF（p, 1）, GF（1,2）, and Thue Morse （TM） PCs, with negative refractive index （NRI） materials. It is found that all of these PCs can open a broad zero-n gap, TM PC possesses the largest zero-n gap, and with the increase of p, the width of the zero-n gap for GF（p, 1） PC becomes smaller. This characteristic is caused by the symmetry of the system and the open position of the zero-n gap. It is found that for GF（p, 1） PCs, the possible limit zero-n gaps open at lower frequencies with the increase of p, but for GF（1,2） and TM PCs, their limit zero-n gaps open at the same frequency. Additionally, for the tbree bottom-bands, we find the interesting perfect self-similarities of the evolution structures with the increase of generation, and obtain the corresponding subband-number formulae. Based on 11 types of evolving manners Qi （i = 1, 2,... , 11） one can plot out the detailed evolution structures of the three kinds of aperiodic PCs for any generation.

关键词： negative refractive index photonic crystals generalized Fibonacci photonic crystal Thue-Morse photonic crystal