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Intrinsically reinforced silks obtained by incorporation of graphene quantum dots into silkworms

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Science China Materials 2019年第2期62卷 245-255页

作者： Lin Ma Maxwell Akologo Akurugu Vivian Andoh Haiyan Liu Jiangchao Song Guohua Wu Long Li College of Biotechnology Jiangsu University of Science and Technology The sericultural Research Institute Chinese Academy of Agricultural Sciences laboratory of risk assessment for sericultural products and edible insects Ministry of Agriculture Department of tea and food technology Jiangsu Polytechnic College Agriculture and Forestry

Silkworm silks have been widely used in a variety of fields due to their sensuousness, luster and excellent mechanical properties. Researchers have paid special attention in improving the mechanical properties of silks. In this work,Bombyx mori larval silkworms are injected with graphene quantum dots(GQDs) through a vascular injection to enhance mechanical properties of the silkworm silks. The GQDs can be incorporated into the silkworm silk gland easily due to hemolymph circulation and influence the spinning process of silkworm. The breaking strength, elongation at break and toughness modulus of the silks increase by 2.74, 1.33 and 3.62 times, respectively, by injecting per individual with 0.6 μg GQDs. Wide-angle X-ray scattering indicates that the size ofβ-sheet nanocrystals in GQDs-silks is smaller than that in control-silks. Infrared spectra suggest that GQDs confine the conformation transition of silk fibroin to β-sheet from random coil/α-helix, and the change of the size and content of β-sheet may be the reason for the improvement of the mechanical properties. The toxicity and safety limit of GQDs incorporated into each silkworm is also evaluated, and the results show that the upmost dose of GQDs per silkworm is30.0 μg. The successful obtainment of reinforced silks by in vivo uptake of GQDs provides a promising route to produce high-strength silks.

关键词： silkworm silks graphene quantum dots decrease of β-sheets enhanced mechanical properties

In vivo toxicity evaluation of boron nitride nanosheets in Bombyx mori silkworm model

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In vivo toxicity evaluation of boron nitride nanosheets in B...

Transcriptome analysis reveals gene expression changes of the silk gland of silkworm（Bombyx mori） in response to MgO NPs treatment

中国蚕学会2021年学术年会

作者： Lin Ma Vivian Andoh Mark Owusu Adjei Haiyan Liu Zhongyuan Shen Long Li Jiangchao Song Weiguo Zhao Guohua Wu College of Biotechnology Jiangsu University of Science and Technology The sericultural Research Institute Chinese Academy of Agricultural Sciences laboratory of risk assessment for sericultural products and edible insects Ministry of Agriculture Department of tea and food technology Jiangsu Polytechnic College of Agriculture and Forestry

Boron nitride nanosheets（BN NSs）,a novel material with a structure similar to graphene,have attracted much attention due to their extraordinary properties.A deep in vivo study of the toxicity of BN NSs is indispensable,which can help to understand their potential risk and provide useful information for their safe ***,so far as we know,the systematic in vivo toxicity evaluation of BN NSs hasn’t been *** this study,silkworm（Bombyx mori） was used as a model to investigate the toxicity of BN NSs,by continuously feeding silkworm larvae with BN NSs at various mass concentrations（1%,2%,3%,4%）.The toxicity was evaluated from the levels of animal entirety（mortality,silkworm growth,cocoons and silk properties）,tissues（pathological examination） and genes（transcriptomic profiling）.The results show that the exposure to BN NSs causes no obvious adverse effects on the growth,silk properties or tissues of silkworm,but the expressions of genes in midgut concerned with some specific functions and pathways are significantly changed,indicating that BN NSs may have potential danger to lead to *** study has performed in vivo toxicity evaluation of BN NSs and provided useful safety information for the application of BN NSs.

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Transcriptome analysis reveals gene expression changes of th...

Size effects of boron nitride nanosheets on the growth and silk properties of silkworm

中国蚕学会2021年学术年会

作者： Lin Ma Vivian Andoh Mark Owusu Adjei Haiyan Liu Zhongyuan Shen Long Li Weiguo Zhao College of Biotechnology Jiangsu University of Science and Technology The sericultural Research Institute Chinese Academy of Agricultural Sciences laboratory of risk assessment for sericultural products and edible insects Ministry of Agriculture Department of tea and food technology Jiangsu Polytechnic College of Agriculture and Forestry

A comparative transcriptome analysis was performed to study the gene expression changes in the silk gland of silkworm after treatment with different concentrations（1%,2%,3% and 4%,mass concentration） of magnesium oxide nanoparticles（MgO NPs）.Compared with control,there are 211 up-regulated and 595 down-regulated genes in MgO NPs *** is observed that 95 genes related to metabolism,23 genes related to immune and 18 genes related to xenobiotics biodegradation were significantly affected by MgO NPs,indicating that MgO NPs have significant influence on the functions of metabolism,immune and xenobiotics biodegradation of silkworm,indicating that MgO NPs may have potential danger to cause *** analysis shows that the interaction between MgO NPs and cells are based on transporter activity,KEGG analysis demonstrates that longevity regulating pathway-worm,peroxisome and MAPK signaling pathway are closely involved in the biological effects of MgO NPs.

关键词： MgO NPs Bombyx mori silk gland transcriptome analysis

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Size effects of boron nitride nanosheets on the growth and s...

Phenolic and flavonoid contents of mandarin (Citrus reticulata Blanco) fruit tissues and their antioxidant capacity as evaluated by DPPH and ABTS methods

中国蚕学会2021年学术年会

作者： Lin Ma Vivian Andoh Hai-Yan Liu College of Biotechnology Jiangsu University of Science and Technology The sericultural Research Institute Chinese Academy of Agricultural Sciences laboratory of risk assessment for sericultural products and edible insects Ministry of Agriculture College of Tea and Food Science Technology Jiangsu Vocational College of Agriculture and Forestry

It is generally known that the biological effects of nanomaterials strongly depend on their *** this study,the boron nitride nanosheets（BN NSs）,a novel material with great potential in biomedical applications,with different sizes are *** kinds of silkworms（qiufeng × baiyu,Nistari 7019） were used as models to investigate the effects of BN NSs with different sizes（BN NSs-1:thickness of 41.5 nm,average diameter of 270.7 nm;BN NSs-2:thickness of 48.2 nm,average diameter of 562.2 nm） on the growth and silk properties of *** results show that exposure to different sized BN NSs causes no obvious adverse effects on the growth or tissues of silkworm,and only BN NSs-1 has some damage to the cocoon of silkworms（cocoon appearance and weight）.XRD,FTIR and SEM results show that the crystalline,secondary or appearance of degummed silks are not *** study has performed size-dependent in vivo biological effects evaluation of BN NSs and provided safety information to enrich the database for better application of BN NSs.

关键词： boron nitride nanosheets silkworm size effect growth silk properties

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Journal of Integrative Agriculture 2018年第1期17卷 256-263页

作者： ZHANG Hua YANG Yi-fei ZHOU Zhi-qin College of Horticulture and Landscape Architecture Southwest University College of Life Science & Engineering Chongqing Three Gorges University Key laboratory of Horticulture for Southern Mountainous Regions Ministry of Education laboratory of Quality & Safety risk assessment for Citrus products of Ministry of Agriculture Citrus Research InstituteSouthwest University

The total phenolic and flavonoid contents in the fruit tissues （peels, pulp residues, seeds, and juices） of 19 citrus genotypes belonged to Citrus reticulata Blanco were evaluated and their antioxidant capacity was tested by 2,2-diphenyl-l-picrylhydra- zyl radicals （DPPH） method and 2,2＇-azino-bis（3-ethylbenzthiozoline-6）-sulphonic acid （ABTS） method. The total phenolic and flavonoid contents, and their antioxidant capacity varied in different citrus fruit tissues. Generally, the peel had both the highest average of total phenolics （27.18 mg gallic acid equivalent （GAE） g^-1 DW） and total flavonoids （38.97 mg rutin equivalent （RE） g^-1 DW）. The highest antioxidant capacity was also the average of DPPH value （21.92 mg vitamin C equiv- alent antioxidant capacity （VCEAC） g^-1 DW） and average of ABTS value （78.70 mg VCEAC g-1 DW） in peel. The correlation coefficient between the total phenolics and their antioxidant capacity of different citrus fruits tissues ranged from 0.079 to 0.792, and from -0.150 to 0.664 for the total flavonoids. The antioxidant capacity of fruit tissues were correlated with the total phenoilc content and flavonoid content except in case of the peel. In addition, the total phenolic content and antioxidant capacity varied in different citrus genotypes. Manju and Karamandarin were better genotypes with higher antioxidation and the phenolic content, however Shagan was the poorest genotype with lower antioxidation and the phenolic content.

关键词： Citrus L. fruit tissues phenolics/flavonoids antioxidant capacity

Rapid authentication of sesame oil using ion mobility spectrometry and chemometrics

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Oil Crop Science 2020年第4期5卷 161-165页

作者： Jun Jiang Xinjing Dou Liangxiao Zhang Jin Mao Li Yua Fei Maa Peiwu Li Oil Crops Research Institute Chinese Academy of Agricultural SciencesWuhanChina Key laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture and Rural AffairsWuhanChina Key laboratory of Detection for Mycotoxins Ministry of Agriculture and Rural AffairsWuhanChina laboratory of risk assessment for Oilseed products(Wuhan) Ministry of Agriculture and Rural AffairsWuhanChina Quality Inspection and Test Center for Oilseed products Ministry of Agriculture and Rural AffairsWuhanChina OCRI-Syscan Joint laboratory of Applications of Ion Mobility Spectrometry on Oilseed and edible Oils WuhanChina

To ensure authenticity of sesame oil,an authentication technology was proposed using ion mobility spectrometry(IMS)and ***-class classification(OCC)methods including one-class partial least squares(OCPLS)and one-class support vector machine(OCSVM)were employed to build authentication models for sesame ***,an independent test set was used to validate the constructed *** set of 45 adulterated oils indicated that prediction correction rate of OCPLS model reached 95.6%(43 out of 45).Moreover,the complete set of sesame oils adulterated by sesame oil essence could be identified as *** with previous studies,OCPLS model could work to identify untargeted *** conclusion,OCC method could effectively detect adulterated sesame oils containing as little as 10%other vegetable *** study provided a rapid screening method for adulterated sesame oil in market surveillance and a reference for developing authentication methods of other edible oils.

关键词： Rapid authentication Sesame oil Ion mobility spectrometry One-class classification

Comparative advantages of chemical compositions of specific edible vegetable oils

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Oil Crop Science 2023年第1期8卷 1-6页

作者： Li Xue Ruinan Yang Xuefang Wang Fei Ma Li Yu Liangxiao Zhang Peiwu Li Key laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture and Rural AffairsLaboratory of Risk Assessment for Oilseed Products(Wuhan)Quality Inspection and Test Center for Oilseed ProductsOil Crops Research InstituteChinese Academy of Agricultural SciencesWuhan430062China College of Food Science and Engineering Nanjing University of Finance and Economics/Collaborative Innovation Center for Modern Grain Circulation and SafetyNanjing210023China Hubei Hongshan laboratory Wuhan430070China Xianghu laboratory Hangzhou311231China

As important supplementary to major edible oils, comparative chemical advantages of minor edible oils decidetheir development and usage. In this study, chemical composition of 13 kinds of specific edible vegetable oilswere investigated. The comparative advantages of chemical compositions of these edible oils were obtained asfollows: (1) camellia, tiger nut and almond oil were rich in oleic acid, the contents of which accounted for79.43%, 69.16% and 66.26%, respectively;(2) safflower oil contained the highest content of linoleic acid(76.69%), followed by grape seed (66.85%) and walnut oil (57.30%);(3) perilla seed, siritch, peony seed andherbaceous peony seed oil were rich in α-linolenic acid (59.61%, 43.74%, 40.83% and 30.84%, respectively);(4)the total phytosterol contents of these oils ranged from 91.46 mg/100 g (camellia oil) to 506.46 mg/100 g (siritchoil);and (5) The best source of tocopherols was sacha inchi oil (122.74 mg/100 g), followed by perilla seed oil(55.89 mg/100 g), peony seed oil (53.73 mg/100 g) and herbaceous peony seed oil (47.17 mg/100 g). Thecomparative advantages of these specific edible oils indicated that they possess the high potential nutritionalvalues and health care functions.

关键词： Comparative advantages Fatty acid composition Phytosterols Tocopherols Specific vegetable oils Chemical composition

edible insects in China： Utilization and prospects

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Insect Science 2018年第2期25卷 184-198页

作者： Ying Feng Xiao-Ming Chen Min Zhao Zhao He Long Sun Cheng-Ye Wang Wei-Feng Ding Research Institute of Resource insects Chinese Academy of Forestry Kunming China

The use of edible insects has a long history in China, where they have been consumed for more than 2000 years. In general, the level of acceptance is high for the consumption of insects in China. Many studies on edible insects have been conducted in the last 20 years, and the scope of the research includes the culture ofentomophagy and the identification, nutritional value, farming and breeding of edible insects, in addition to food production and safety. Currently, 324 species of insects from 11 orders are documented that are either edible or associated with entomophagy in China, which include the common edible species, some less commonly consumed species and some medicinal insects. How- ever, only approximately 10 to 20 types of insects are regularly consumed. The nutritional values for 174 species are available in China, including edible, feed and medicinal species. Although the nutritional values vary among species, all the insects examined contain pro- tein, fat, vitamins and minerals at levels that meet human nutritional requirements. edible insects were, and continue to be, consumed by different ethnic groups in many parts of China. People directly consume insects or food products made from insects. The processing of products from insect protein powder, oil and chitin, and the development of healthcare foods has been studied in China. People also consume insects indirectly by eating livestock that were fed insects, which may be a more acceptable pathway to use insects in human diets. Although limited, the data on the food safety of insects indicate that insects are safe for food or feed. Incidences of allergic reactions after consuming silkworm pupae, cicadas and crickets have been reported in China. Insect farming is a unique breeding industry in rural China and is a source of income for local people. insects are reared and bred for human food, medicine and animal feed using two approaches in China： the insects are either fully domesticated and reared comple

关键词： edible insects entomophagy nutritive value insect food safety insectfarming

Research progress on the origin traceability of edible oils

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Oil Crop Science 2020年第4期5卷 194-197页

作者： Qian Zhou Xinjing Dou Liangxiao Zhang Wei Fan Shiyin Guo Weiguo Wu Zhonghai Tang College of Food Science and Technology Hunan Agricultural UniversityChangsha410128China Oil Crops Research Institute Chinese Academy of Agricultural SciencesWuhan430062China laboratory of Quality and Safety risk assessment for Oilseeds products(Wuhan) Ministry of Agriculture and Rural AffairsWuhan430062China Quality Inspection and Test Center for Oilseeds products Ministry of Agriculture and Rural AffairsWuhan430062China Hunan Engineering Technology Research Center for Rapeseed Oil Nutrition Health and Deep Development Changsha410128China

The origin traceability of edible oil is an effective way to protect the legitimate rights and interests of producers and *** review briefly summarizes research progress on origin traceability technologies for edible oils including stable isotope ratio mass spectrometry,mineral element fingerprint analysis,organic component fingerprint analysis,near-infrared spectroscopy,nuclear magnetic resonance technology and biochemical ***,the development trends in edible oil origin traceability technology were proposed.

关键词： edible oil Origin traceability Stable isotope ratio Mineral element fingerprint Organic composition