检索结果-南通市图书馆

The glutamate receptor gene GLR3.3:A bridge of calciummediated root development in poplar

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Horticultural plant Journal 2024年第6期10卷 1449-1462页

作者： Yi An Ya Geng Yu Liu Xiao Han Lichao Huang Wei Zeng Jin Zhang Mengzhu Lu State Key laboratory of Subtropical Silviculture Sino-Australia Plant Cell Wall Research CentreCollege of Forestry and BiotechnologyZhejiang A&F UniversityHangzhouZhejiang 311300China

Poplar is one of the fastest-growing temperate trees in the world and is widely used in ornamental horticulture for *** root is essential for tree growth and development and its utilization potential is ***(Ca),as a signaling molecule,is involved in the regulation of plant root ***,the detailed underlying regulatory mechanism is *** this study,we analyzed the morphological and transcriptomic variations of 84K poplar(Populus alba×***)in response to different calcium concentrations and found that low Ca^(2+)(1 mmol·L^(-1))promoted lateral root development,while deficiency(0.1 mmol·L^(-1)Ca^(2+))inhibited lateral root ***-expression analysis showed that Ca^(2+)channel glutamate receptors(GLRs)were present in various modules with significance for root *** GLR paralogous genes,PagGLR3.3a and Pag GLR3.3b,were mainly expressed in roots and up-regulated under Ca^(2+)*** CRISPR/Cas9-mediated signal gene(crispr-PagGLR3.3a,PagGLR3.3b)and double gene(crispr-PagGLR3.3ab)mutants presented more and longer lateral *** analysis showed that crispr-PagGLR3.3ab plants had more xylem cells and promoted the development of secondary vascular *** transcriptomic analysis suggested that knockout of PagGLR3.3a and PagGLR3.3b led to the up-regulation of several genes related to protein phosphorylation,auxin efflux,lignin and hemicellulose biosynthesis as well as transcriptional regulation,which might contribute to lateral root *** study not only provides novel insight into how the Ca^(2+)channels mediated root growth and development in trees,but also provides a directive breeding of new poplar species for biofuel and bioenergy production.

关键词： Glutamate receptor Calcium Root development Lateral root Poplar

Efficient Transformation of indica Rice Mediated by Agrobacterium and Generation of NcGDH Transgenic Genic Male-Sterile Rice with High Nitrogen Use Efficiency

在线全文

学校读者我要写书评

暂无评论

Rice science 2021年第6期28卷 529-531,I0030-I0036页

作者： LIU Cong TANGg Dongying ZHOU Zhengkun ZENG Hui HU Xiaochun TAN Vanning QIN Peng DENG Yong WU Jicai WANG Yan YANG Yuanzhu YUAN Dingyang LIU Xuanming LIN Jianzhong Hunan province Key laboratory of plant Functional Genomics and Developmental regulation College of BiologyHunan UniversityChangsha 410082China Academy of Seed Industry of Hunan Yahua Changsha 410001China State Key laboratory of Hybrid Rice Hunan Hybrid Rice Research CenterHunan Academy of Agricultural SciencesChangsha 410125China

Although some indica rice varieties have already been transformed by Agrobacterium,there is still great demand of developing transformation system for economically important indica ***,a fast and efficient method of Agrobacterium-mediated transformation for indica rice was developed,and an NcGDH transgenic genic male-sterile(GMS)indica rice 628S was generated using this *** regenerated transgenic plantlets can be obtained within three months,and the transformation efficiency achieved about 17%.NcGDH transgenic 628S exhibited an enhanced capacity of ammonium assimilation and more effective panicles under low nitrogen *** the hybrid rice lines generated by NcGDH transgenic 628S showed 8%-27%yield advantage than those by the non-transgenic *** together,these results provide an efficient transfbnnation method for indica rice and an opportunity to improve nitrogen use efficiency(NUE)and the heterosis of hybrid rice.

关键词： sterile Transformation transformation

The DUF579 proteins GhIRX15s regulate cotton fiber development by interacting with proteins involved in xylan synthesis

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2024年第4期12卷 1112-1125页

作者： Mengyun Li Feng Chen Jingwen Luo Yanan Gao Jinglong Cai Wei Zeng Monika S.Doblin Gengqing Huang Wenliang Xu Hubei Key laboratory of Genetic regulation and Integrative Biology School of Life SciencesCentral China Normal UniversityWuhan 430079HubeiChina zhejiang Provincial Key laboratory of plant Evolutionary Ecology and Conservation School of Life SciencesTaizhou UniversityTaizhou 318000ZhejiangChina La Trobe Institute for Sustainable Agriculture&Food School of AgricultureBiomedicine and EnvironmentLa Trobe UniversityBundooraVIC 3086Australia

Cotton provides the most abundant natural fiber for the textile *** mature cotton fiber largely consists of secondary cell walls with the highest proportion of cellulose and a small amount of hemicellulose and *** dissect the roles of hemicellulosic polysaccharides during fiber development,four IRREGULAR XYLEM 15(IRX15)genes,GhIRX15-1/-2/-3/-4,were functionally characterized in *** genes encode DUF579 domain-containing proteins,which are homologs of AtIRX15 involved in xylan *** four GhIRX15 genes were predominantly expressed during fiber secondary wall thickening,and the encoded proteins were localized to the Golgi *** GhIRX15 gene could restore the xylan deficient phenotype in the Arabidopsis irx15irx15l double *** of GhIRX15s in cotton resulted in shorter mature fibers with a thinner cell wall and reduced cellulose content as compared to the wild ***,GhIRX15-2 and GhIRX15-4 formed homodimers and *** addition,the GhIRX15s showed physical interaction with glycosyltransferases GhGT43C,GhGT47A and GhGT47B,which are responsible for synthesis of the xylan backbone and reducing end ***,the GhIRX15s can form heterocomplexes with enzymes involved in xylan modification and side chain synthesis,such as GhGUX1/2,GhGXM1/2 and *** findings suggest that GhIRX15s participate in fiber xylan biosynthesis and modulate fiber development via forming large multiprotein complexes.

关键词： Cotton fiber Xylan biosynthesis GhIRX15s Protein-protein interaction Protein complexes

Functional evaluation of the insulin/insulin-like growth factor signaling pathway in determination of wing polyphenism in pea aphid

在线全文

学校读者我要写书评

暂无评论

Insect Science 2023年第3期30卷 816-828页

作者： Yiyang Yuan Yanyan Wang Wanwan Ye Erliang Yuan Jian Di Xin Chen Yanling Xings Yucheng Sun Feng Ge Institute of plant Protection Shandong Academy of Agricultural SciencesJinanChina State Key laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBejingChina Shandong province Key laboratory of plant Virology JinanChina School of Life Sciences Hebei UniversityBaodingHebei ProvinceChina College of Life Sciences Cangzhou Normal University CangzhouHebei ProvinceChina

Wing polyphenism is a common phenomenon that plays key roles in environmental adaptation of ***/insulin-like growth factor signaling(IIS)pathway is a highly conserved pathway in regulation of metabolism,development,and growth in *** has been reported that IS is required for switching of wing morph in brown planthopper via regulating the development of the wing ***,it remains elusive whether and how IIS pathway regulates transgenerational wing dimorphism in *** this study,we found that pairing and solitary treatments can induce pea aphids to produce high and low percentage winged offspring,*** expression level of ILP5(insulin-like peptide 5)in maternal head was significantly higher upon solitary treatment in comparison with pairing,while silencing of ILP5 caused no obvious change in the winged offspring *** interference-mediated knockdown of FoxO(Forkhead transcription factor subgroup O)in stage 20 embryos significantly increased the winged offspring *** results of pharmacological and quantitative polymerase chain reaction experiments showed that the embryonic insulin receptors may not be involved in wing ***,ILP4 and ILP11 exhibited higher expression levels in 1st wingless offspring than in winged *** demonstrate that FoxO negatively regulates the wing morph development in *** may regulate aphid wing polyphenism in a developmental stage-specific ***,the regulation may be not mediated by the canonical IIS *** findings advance our understanding of IIS pathway in insect transgenerational wing polyphenism.

关键词： Acyrthosiphon pisum FoxO insulin/insulin-like growth factor signaling pathway insulin-like peptides wing polyphenism

Temperature Difference Between the Air and Organs of Rice plant and Its Relation to Spikelet Fertility

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2008年第6期7卷 678-685页

作者： YAN Chuan DING Yan-feng LIU Zheng-hui WANG Qiang-sheng LI Gang-hua HE Ying WANGShao-hua Key laboratory of Crop Growth regulation Ministry of Agriculture/Hi-Tech Key Laboratory of Information Agriculture of Jiangsu Province Nanjing Agricultural University Nanjing 210095 P.R.China

Based on the experiment of measuring panicles and leaves, air temperature, and humidity above the canopy of rice cultivars after heading in 2005 and 2006, we investigated the temperature difference （TD） between the air and organs of rice plant and its relationship with spikelet fertility. The results showed that TDs between the air and organs of rice varied with air temperature, air humidity, and plant type. For similar air humidity, TDs were lower at the air temperature of 28.5℃ than at higher temperature of 35.5℃, whereas for the same air temperature, the TDs decreased as the air humidity increased. TDs were also affected by plant type of the cultivars. Erect panicle cultivars showed higher TDs than those with droopy panicles under similar climatic conditions, and cultivars with panicles above flag leaf （PAFL） had higher TDs than those with panicles below the flag leaf （PBFL）. Cultivars grown in a location with lower air humidity and higher temperature, such as Taoyuan, China, had higher spikelet fertility than those in higher humidity under the similar air temperature during the grain filling stage. This is partially attributed to the larger TDs under the lower humidity. Rowspacing and the ratio of basal-tillering to panicle-spikelet fertilizer showed a significant influence on TD and subsequently on spikelet fertility, suggesting the possibility of increasing spikelet fertility by agronomic management.

关键词： rice air temperature temperature of organs spikelet fertility

Genome-wide identification and analysis of the regulation wheat DnaJ family genes following wheat yellow mosaic virus infection

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2022年第1期21卷 153-169页

作者： LIU Ting-ting XU Miao-ze GAO Shi-qi ZHANG Yang HU Yang JIN Peng CAI Lin-na CHENG Ye CHEN Jian-ping YANG Jian ZHONG Kai-li State Key laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products/Key laboratory of Biotechnology in plant Protection of Ministry of Agriculture and Rural Affairs and zhejiang province Institute of Plant VirologyNingbo UniversityNingbo 315211P.R.China Institutes of Biomedical Sciences Fudan UniversityShanghai 200032P.R.China Institute of Forest Health Zhejiang Academy of ForestryHangzhou 310021P.R.China

The co-chaperone DnaJ plays an important role in protein folding and regulation of various physiological activities, and participates in several pathological processes. DnaJ has been extensively studied in many species including humans,drosophila, mushrooms, tomatoes, and Arabidopsis. However, few studies have examined the role of DnaJ in wheat(Triticum aestivum), and the interaction mechanism between TaDnaJs and plant viruses. Here, we identified 236 TaDnaJs and performed a comprehensive genome-wide analysis of conserved domains, gene structure and protein motifs, chromosomal positions and duplication relationships, and cis-acting elements. We grouped these Ta Dna Js according to their domains, and randomly selected six genes from the groups for tissue-specific analysis, and expression profiles analysis under hormone stress, and 17 genes for plant virus infection stress. In qRT-PCR, we found that among the 17 TaDnaJ genes tested, 16 genes were up-regulated after wheat yellow mosaic virus(WYMV) infection, indicating that the TaDnaJ family is involved in plant defense response. Subsequent yeast two-hybrid assays verified the WYMV NIa, NIb and 7 KD proteins interacted with TaDJC(TraesCS7 A02 G506000), which had the most significant changes in gene expression levels after WYMV *** into the molecular mechanisms of Ta Dna J-mediated stress tolerance and sensitivity could inform different strategies designed to improve crop resistance to abiotic and biotic stress. This study provides a basis for future investigation of the TaDnaJ family and plant defense mechanisms.

关键词： TaDnaJ WYMV wheat genome-wide expression hormone biotic stress HSP40

Nitric oxide mediates the fungal elicitor-induced puerarin biosynthesis in Pueraria thomsonii *** cells through a salicylic acid(SA)-dependent and a jasmonic acid(JA)-dependent signal pathway

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2006年第4期49卷 379-389页

作者： XU Maojun1, DONG Jufang1 & ZHU Muyuan2 Department of Biotechnology, zhejiang Gongshang University, Hangzhou 310035, China State Key laboratory of plant Physiology and Biochemistry, College of Life Sciences, zhejiang University, Hangzhou 310012, China Department of Biotechnology Zhejiang Gongshang University Hangzhou China State Key laboratory of plant Physiology and Biochemistry College of Life Sciences Zhejiang University Hangzhou China

Nitric oxide (NO) has emerged as a key signaling molecule in plant secondary metabolite biosynthesis recently. In order to investigate the molecular basis of NO signaling in elicitor-induced secondary metabolite biosynthesis of plant cells, we determined the contents of NO, salicylic acid (SA), jasmonic acid (JA), and puerarin in Pueraria thomsonii Benth. suspension cells treated with the elicitors prepared from cell walls of Penicillium citrinum. The results showed that the fungal elicitor induced NO burst, SA accumulation and puerarin production of P. thomsonii Benth. cells. The elicitor-induced SA accumulation and puerarin production was suppressed by nitric oxide specific scavenger cPITO, indicating that NO was essential for elicitor-induced SA and puerarin biosynthesis in P. thomsonii Benth. cells. In transgenic NahG P. thomsonii Benth. cells, the fungal elicitor also induced puerarin biosynthesis, NO burst, and JA accumulation, though the SA biosynthe-sis was impaired. The elicitor-induced JA accumulation in transgenic cells was blocked by cPITO, which suggested that JA acted downstream of NO and its biosynthesis was controlled by NO. External application of NO via its donor sodium nitroprusside (SNP) enhanced puerarin biosynthesis in trans-genic NahG P. thomsonii Benth. cells, and the NO-triggered puerarin biosynthesis was suppressed by JA inhibitors IBU and NDGA, which indicated that NO induced puerarin production through a JA-dependent signal pathway in the transgenic cells. Exogenous application of SA suppressed the elicitor-induced JA biosynthesis and reversed the inhibition of IBU and NDGA on elicitor-induced pu-erarin accumulation in transgenic cells, which indicated that SA inhibited JA biosynthesis in the cells and that SA might be used as a substitute for JA to mediate the elicitor- and NO-induced puerarin biosynthesis. It was, therefore, concluded that NO might mediate the elicitor-induced puerarin bio-synthesis through SA- and JA-dependent signal p

关键词： nitric oxide (NO), salicylic acid (SA), jasmonic acid (JA), fungal elicitor, puerarin.

SIMYB1 and SIMYB2, two new MYB genes from tomato, transcriptionally regulate cellulose biosynthesis in tobacco

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2017年第1期16卷 65-75页

作者： SHI Yan-na LIU Xiao-fen LI Xue DONG Wen-cheng Donald Grierson YIN Xue-ren CHEN Kun-song College of Agriculture & Biotechnology Zhejiang University Hangzhou 310058 P.R. China zhejiang Provincial Key laboratory of Horticultural plant Integrative Biology Zhejiang University Hangzhou 310058 P.R.China laboratory of Horticultural plant Growth Development and Quality Improvement Ministry of Agriculture Zhejiang University Hangzhou 310058 P.R. China plant & Crop Sciences Division School of Biosciences University of Nottingham Sutton Bonington Campus Loughborough LE12 5RD UK

Cellulose, a major constituent of plant biomass, is synthesized by a cellulose synthase complex. It has been demonstrated that MYB genes transcriptionally regulate cellulose synthase in Arabidopsis. However, little is known about this process in tomato. Here, two MYB （SIMYB1/2） and three cellulose synthase （CESA）（SICESA41516） genes were isolated. SIMYB1/2 and SICESA4/5/6 accumulation was found to correspond to cellulose accumulation in different tissues of tomato. Dual luciferase assays indicated that these two MYBs were transcriptional activators that interact with promoters of SICESA4/5/6. Moreover, SIMYB2 could also activate promoters of SIMYB1/2, suggesting the possible underlying auto-activation mech- anisms for MYB transcription factors. Transient over-expression of SlMYB1/2 in Nicotiana tabacum up-regulated tobacco endogenous NtCESA genes and increased cellulose accumulation. The function of SIMYB112 was further investigated using stable transformation and the results indicated that N. tabacum lines heterologous expressing SIMYB1/2 displayed a pleiotropic phenotype, long and narrow leaves, with NtCESA induced and significant increase of cellulose. In conclusion, our data suggest that tomato SIMYB1/2 have transcriptional regulatory roles in cellulose biosynthesis and SIMYB2 was more effective than SIMYB1, which may due to the transcriptional activation by SIMYB2 on SIMYB1 and itself.

关键词： MYB cellulose cellulose synthase transcriptional regulation tomato tobacco

ZmMYC7 directly regulates ZmERF147 to increase maize resistance to Fusarium graminearum

在线全文

学校读者我要写书评

暂无评论

The Crop Journal 2023年第1期11卷 79-88页

作者： Hongzhe Cao Kang Zhang Wei Li Xi Pang Pengfei Liu Helong Si Jinping Zang Jihong Xing Jingao Dong State Key laboratory of North China Crop Improvement and regulation Hebei Agricultural UniversityBaoding 071000HebeiChina Hebei Key laboratory of plant Physiology and Molecular Pathology Hebei Agricultural UniversityBaoding 071000HebeiChina

The jasmonic acid(JA)signaling pathway is involved in plant growth,development,and response to abiotic or biotic ***2,a bHLH transcription factor,is a regulatory hub in the *** function of ZmMYC7,a putative MYC2 ortholog,in jasmonate-signaled defense responses of maize has not been *** this study,we found that ZmMYC7 possesses JID,TAD,bHLH and Zip domains and essential characteristics of transcription factors:a nuclear location and transactivation *** ZmMYC7mutants showed markedly increased sensitivity to Fusarium graminearum and Setosphaeria *** expression levels of the defense-associated genes ZmPR1,ZmPR2,ZmPR3,ZmPR5,ZmPR6,and ZmPR7 in response to *** infection were downregulated in ZmMYC7 mutants,while ZmPR4 and ZmPR10 were ***7 interacted with members of the ZmJAZ family,including ZmJAZ8,ZmJAZ11,and ***7 physically interacted with G-box cis-elements in the ZmERF147 promoter in vitro and transcriptional activation of ZmERF147 by ZmMYC7 was inhibited by ZmJAZ11 and ***147 mutants were more susceptible to *** infection than inbred line B73with concomitant down-regulation of all defense-associated ZmPRs except *** findings indicate that ZmMYC7 functions in maize resistance to *** and sheds light on maize defense responses to pathogenic fungi via the JA signaling pathway.

关键词： Maize Fusarium graminearum ZmMYC7 ZmERF147