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Relations between mercury fractions and microbial community components in seawater under the presence and absence of probable phosphorus limitation conditions

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Journal of Environmental Sciences 2019年第1期31卷 145-162页

作者： Igor ?ivkovi? Vesna Fajon Jo?e Kotnik Yaroslav Shlyapnikov Kristina Obu Vazner Ermira Begu Stefanija ?estanovi? Danijela ?anti? Ana Vrdoljak Slaven Jozi? Mladen ?oli? Jelena Lu?i? Jere Ve?a Grozdan Ku?pili? Marin Ordulj Frano Mati? Branka Grbec Natalia Bojani? ?ivana Nin?evi? Gladan Milena Horvat Department of Environmental Sciences Jo?ef Stefan Institute Jo?ef Stefan International Postgraduate School Ecological Engineering Institute Maribor 2000 Slovenia laboratory of Marine Microbiology Institute of Oceanography and FisheriesSplit 21000 Croatia laboratory of Chemical Oceanography and Sedimentology of the Sea Institute of Oceanography and FisheriesSplit 21000 Croatia laboratory of Chemical Oceanography and Sedimentology of the Sea Institute of Oceanography and Fisheries Department of Marine Studies University of SplitSplit 21000 Croatia laboratory of Physical Oceanography Institute of Oceanography and FisheriesSplit 21000 Croatia Laboratory of Plankton and Shellfish Toxicity Institute of Oceanography and FisheriesSplit 21000 Croatia

Microbial transformations of toxic monomethylmercury(MMHg) and dissolved gaseous mercury(DGM) at the lower levels of the marine food web are not well understood, especially in oligotrophic and phosphorus-limited seas. To examine the effects of probable phosphorus limitation(~PP-limitation) on relations between mercury(Hg) fractions and microorganisms, we determined the total mercury(THg), total methylated mercury(MeHg), DGM, and microbiological and chemical parameters in the Central Adriatic Sea. Using statistical analysis, we assessed the potential microbial effects on Hg transformations and bioaccumulation. Only in the absence of ~PP-limitation conditions(^(NO–P)P-limitation) is MeHg significantly related to most chemical and microbial parameters, indicating metabolism-dependent Hg transformations.The heterotrophic activity of low nucleic acid bacteria(abundant in oligotrophic regions)seems responsible for most of Hg methylation under ^(NO–P)P-limitation. Under these conditions,DGM is strongly related to microbial fractions and chlorophyll a, indicating biological DGM production, which is probably not metabolically induced, as most of these relations are also observed underPP-limitation. MMHg biomagnification was observed through an increased bioaccumulation factor from microseston to mesozooplankton. Our results indicate that Hgtransformations and uptake might be enhanced under ^(NO–P)P-limitation conditions, emphasizing their impact on the transfer of Hg to higher trophic levels.

关键词： Mercury speciation Methylmercury Probable phosphorus limitation Microbial community

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Dietary cholesterol promotes growth and ecdysone signalling pathway by modulating cholesterol transport in swimming crabs(Portunus trituberculatus)

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Animal Nutrition 2022年第3期10卷 249-260页

作者： Tingting Zhu Qicun Zhou Zheng Yang Yingying Zhang Jiaxiang Luo Xiangsheng Zhang Yuedong Shen Lefei Jiao Douglas R.Tocher Min Jin laboratory of Fish and shellfish Nutrition School of Marine SciencesNingbo University315211 NingboChina Guangdong Provincial Key laboratory of Marine Biotechnology Institute of Marine SciencesShantou University515063 ShantouChina

Cholesterol,as an indispensable nutrient,regulates molting and growth in crustacean.As crustaceans are unable to biosynthesize cholesterol de novo,it is central to understand how dietary cholesterol affects molting in crustaceans.An 8-week feeding trial was conducted to evaluate the effects of dietary cholesterol level(0.12%,0.43%,0.79%,1.00%,1.30%and 2.50%)on growth,cholesterol metabolism and expression of genes related to lipid and ecdysone metabolism in female swimming crabs(Portunus trituberculatus).A total of 192 crabs(1.41±0.05 g)were randomly distributed into 192 aquaria.Each treatment had 4 replicates with each replicate containing 8 crabs.Crabs fed the 1.00%cholesterol diet showed best growth performance,and thus based on percent weight gain,the optimal dietary cholesterol requirement was calculated at 1.01%.Tissue cholesterol concentrations were positively correlated with dietary cholesterol level.The contents of functional fatty acids in hepatopancreas significantly increased as dietary cholesterol increased from 0.12%to 2.50%(P<0.05).The expression levels of genes related to lipogenesis pathway,lipid catabolism and fatty acid oxidation were significantly downregulated with increased dietary cholesterol level(P<0.05).The highest expression levels of cholesterol transport genes,low-density lipoprotein receptor(ldlr)and low-density lipoprotein receptorrelated protein 2(lrp2)occurred in crabs fed the 1.30%cholesterol diet.Moreover,hormones related to molting such as crustacean hyperglycemic hormone(CHH),methyl farnesoate(MF),molt-inhibiting hormone(MIH),and ecdysone in hemolymph were significantly influenced by dietary cholesterol level(P<0.05).The highest expression levels of ecdysone receptor(ecr)and chitinase 1(chi1)in eyestalk and hepatopancreas were found in crabs fed the diet containing 1.00%cholesterol(P<0.05).In conclusion,the optimal dietary level was beneficial to functional fatty acid accumulation,regulated lipid metabolism,promoted the ecdysone signalling pathway by improving the cholesterol t

关键词： Portunus trituberculatus Cholesterol Lipid metabolism Ecdysone signalling pathway

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High dietary lipid level promotes low salinity adaptation in the marine euryhaline crab(Scylla paramamosain)

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Animal Nutrition 2023年第1期12卷 297-307页

作者： Jiaxiang Luo Chen Ren Tingting Zhu Chen Guo Shichao Xie Yingying Zhang Zheng Yang Wenli Zhao Xiangsheng Zhang Jingjing Lu Lefei Jiao Qicun Zhou Douglas R.Tocher Min Jin laboratory of Fish and shellfish Nutrition School of Marine SciencesNingbo UniversityNingbo 315211China Guangdong Provincial Key laboratory of Marine Biotechnology Institute of Marine SciencesShantou UniversityShantou 515063China

The physiological processes involved in adaptation to osmotic pressure in euryhaline crustaceans are highly energy demanding,but the effects of dietary lipids(fat)on low salinity adaptations have not been well evaluated.In the present study,a total of 120 mud crabs(Scylla paramamosain,BW=17.87±1.49 g)were fed control and high-fat(HF)diets,at both medium salinity(23‰)and low salinity(4‰)for 6 wk,and each treatment had 3 replicates with each replicate containing 10 crabs.The results indicated that a HF diet significantly mitigated the reduction in survival rate,percent weight gain and feed efficiency induced by low salinity(P<0.05).Low salinity lowered lipogenesis and activated lipolysis resulting in lipid depletion in the hepatopancreas of mud crabs(P<0.05).Thus,HF diets enhanced the process of lipolysis to supply more energy.In the gills,low salinity and the HF diet increased the levels of mitochondrial biogenesis markers,the activity of mitochondrial complexes,and the expression levels of genes related to energy metabolism(P<0.05).Consequently,the positive effects of the HF diet on energy metabolism in mud crabs at low salinity promoted osmotic pressure regulation.Specifically,significantly higher haemolymph osmotic pressure and inorganic ion content,as well as higher osmotic pressure regulatory enzyme activity in gills,and gene and protein expression levels of NaK-ATPase were observed in crabs fed the HF diet at low salinity(P<0.05).In summary,high dietary lipid levels improved energy provision to facilitate mitochondrial biogenesis,which increased ATP provision for osmotic pressure regulation of mud crabs.This study also illustrates the importance of dietary lipid nutrition supplementation for low salinity adaptations in mud crabs.

关键词： Osmotic pressure regulation Mitochondrial energy metabolism Lipid metabolism

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Effects of Dietary Vitamin D_(3)on Growth Performance,Immune Status,and Calcium and Phosphorus Metabolism in Litopenaeus vannamei Under Different Salinity Conditions

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Journal of Ocean University of China 2023年第4期22卷 1023-1033页

作者： LIU Miao ZHANG Hui SHAO Rui ZHOU Xueqi ZHOU Qicun MAI Kangsen AI Qinghui WAN Min Key laboratory of Aquaculture Nutrition and Feed Ministry of Agriculture&Key Laboratory of MaricultureMinistry of EducationCollege of FisheriesOcean University of ChinaQingdao 266003China laboratory of Fish and shellfish Nutrition School of Marine SciencesNingbo UniversityNingbo 315211China

Vitamin D_(3)(VD_(3)) plays a vital role in various physiological processes in addition to its role in regulating the homeostasis of calcium and phosphorus.The present study was conducted to investigate the effects of dietary VD_(3) on growth performance,immune status and the metabolism of calcium and phosphorus in Litopenaeus vannamei under the optimal salinity(25)and high salinity(35)conditions.Shrimp(2.1 g±0.1 g)were fed with experimental diets containing 0,1500,6000 and 12000IUkg^(−1)VD_(3) for 30 days under two salinity conditions respectively.The results showed that the growth performance and intestinal health were reduced,while oxidative stress was induced in the shrimp cultured at salinity 35 compared to those in the shrimp cultured at salinity 25.Interestingly,dietary supplementation of 1500 IU kg^(−1)VD_(3) at salinity 35 promoted the growth performance,improved the intestinal health,and enhanced the antioxidant capacity and non-specific immunity in shrimp.In addition,the content of inorganic phosphorus in shrimp serum was affected by dietary VD_(3) independent of salinity.In contrast,the expressions of calcium transport-related genes,including calmodulin and Ca2+-ATPase,were regulated by salinity,instead of dietary VD_(3).In conclusion,an appropriate supplementation of dietary VD_(3) under high-salt condition can promote the growth,alleviate intestinal inflammation,enhance antioxidant capacity and immunity,and contribute to phosphorus metabolism in L.vannamei.

关键词： vitamin D_(3) Penaeus vannamei salinity antioxidant capacity immunity metabolism intestinal health

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The potential of ocean acidification on suppressing larval development in the Pacific oyster Crassostrea gigas and blood cockle Area inflata Reeve

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Chinese Journal of Oceanology and Limnology 2014年第6期32卷 1307-1313页

作者：李加琦蒋增杰张继红毛玉泽卞大鹏方建光 Mariculture Ecology and Carrying Capacity laboratory Yellow Sea Fisheries Research InstituteChinese Academy of Fisheries Sciences National Engineering Research Center of Marine shellfish

We evaluated the effect of pH on larval development in larval Pacific oyster(Crassostrea gigas) and blood cockle(Arca inflata Reeve).The larvae were reared at pH 8.2(control),7.9,7.6,or 7.3beginning 30 min or 24 h post fertilization.Exposure to lower pH during early embryonic development inhibited larval shell formation in both species.Compared with the control,larvae took longer to reach the D-veliger stage when reared under pH 7.6 and 7.3.Exposure to lower pH immediately after fertilization resulted in significantly delayed shell formation in the Pacific oyster larvae at pH 7.3 and blood cockle larvae at pH 7.6 and 7.3.However,when exposure was delayed until 24 h post fertilization,shell formation was only inhibited in blood cockle larvae reared at pH 7.3.Thus,the early embryonic stages were more sensitive to acidified conditions.Our results suggest that ocean acidification will have an adverse effect on embryonic development in bivalves.Although the effects appear subtle,they may accumulate and lead to subsequent issues during later larval development.

关键词：太平洋牡蛎幼虫发育泥蚶酸化海洋早期胚胎发育低pH值幼虫饲养

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Dietary choline activates the Ampk/Srebp signaling pathway and decreases lipid levels in Pacific white shrimp(Litopenaeus vannamei)

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Animal Nutrition 2023年第4期15卷 58-70页

作者： Jingjing Lu Xinyue Tao Jiaxiang Luo Tingting Zhu Lefei Jiao Peng Sun Qicun Zhou Douglas R.Tocher Min Jin laboratory of Fish and shellfish Nutrition School of Marine SciencesNingbo UniversityNingbo 315211China Guangdong Provincial Key laboratory of Marine Biotechnology Institute of Marine SciencesShantou UniversityShantou 515063China

An 8-week feeding trial was conducted in Pacific white shrimp(Litopenaeus vannamei)to evaluate the effects of dietary choline supplementation on choline transport and metabolism,hepatopancreas histological structure and fatty acid profile,and regulation of lipid metabolism.Six isonitrogenous and isolipidic diets were formulated to contain different choline levels of 2.91(basal diet),3.85,4.67,6.55,10.70 and 18.90 g/kg,respectively.A total of 960 shrimp(initial weight,1.38±0.01 g)were distributed randomly into twenty-four 250-L cylindrical fiber-glass tanks,with each diet assigned randomly to 4replicate tanks.The results indicated that dietary choline significantly promoted the deposition of choline,betaine and carnitine(P<0.05).The diameters and areas of R cells,total lipid and triglyceride contents in hepatopancreas,and triglyceride and non-esterified fatty acid contents in hemolymph were negatively correlated with dietary choline level.The contents of functional fatty acids in hepatopancreas,the activity of acetyl-CoA carboxylase(Acc),and the mRNA expression of fas,srebp and acc were highest in shrimp fed the diet containing 4.67 g/kg choline,and significantly higher than those fed the diet containing 2.91 g/kg,the lowest level of choline(P<0.05).The number of R cells,content of very lowdensity lipoprotein(VLDL),activities of carnitine palmitoyl-transferase(Cpt1),lipoprotein lipase and hepatic lipase,and the mRNA expression levels of cpt1,fabp,fatp,ldlr,and ampk in hepatopancreas increased significantly as dietary choline increased(P<0.05).In addition,hepatopancreas m RNA expression levels of ctl1,ctl2,oct1,badh,bhmt,ck,cept,and cct were generally up-regulated as dietary choline level increased(P<0.01).In conclusion,dietary choline promoted the deposition of choline and its metabolites by up-regulating genes related to choline transport and metabolism.Moreover,appropriate dietary choline level promoted the development of hepatopancreas R cells and maintained the normal accumulation of lipids required for develo

关键词： Pacific white shrimp Choline Lipid Fatty acid Histology Ampk signaling pathway

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Myo-inositol accelerates the metamorphosis from megalopa to crablet of Scylla paramamosain by modulating cuticle decomposition and reconstruction

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Aquaculture and Fisheries 2023年第6期8卷 695-705页

作者： Wenxiao Cui Hafiz Sohaib Ahmed Saqib Weifeng Gao Ruibo Wang Yang He Yang Yu Zhen Lin Qingyu Zhang Yin Zhang Shengkang Li Huaiping Zheng Yueling Zhang Mhd Ikhwanuddin Hongyu Ma Guangdong Provincial Key laboratory of Marine Biotechnology Shantou UniversityShantou515063China STU-UMT Joint shellfish Research laboratory Shantou UniversityShantou515063China Rongtong Agricultural Development(Guangzhou)Co. LtdGuangzhou510030China Institute of Tropical Aquaculture and Fisheries Universiti Malaysia TerengganuKuala Nerus21030Malaysia

The mud crab Scylla paramamosain is a key species in China due to its high nutritional value and great economic worth and has grown in popularity.Myo-inositol can modulate versatile physiological functions in aquatic animals.In the present study,S.paramamosain megalopa were given graded concentrations of myo-inositol(0,1,2,4,and 8 ppm)by water immersion to explore how their metamorphosis would be affected.The results showed that supplementing with myo-inositol remarkably increased transformation and survival rate from megalopa to crablet by at least 1.16 and 1.26 times,respectively.To decipher the molecular mechanism of how myo-inositol increases metamorphosis and survival rate,we further performed transcriptome-based gene expression profiling of both megalopa and crablet treated with myo-inositol.The integrative transcriptome analyses predicted that the differentially expressed genes(DEGs)were significantly enriched in chitinase activity,structural constituent of cuticle,and chitin binding,which are associated with the decomposition and reconstruction of cuticle.qPCR results confirmed that myo-inositol mediated gene expression levels of the above cuticle-related pathways.Considering the importance of the cuticle in exoskeleton formation and molting,it can be concluded that myo-inositol-induced changes in the cuticle decomposition and reconstruction might have accelerated the transformation from megalopa to crablet of S.paramamosain.Besides,numerous DEGs were significantly enriched in protein digestion and absorption,amino sugar and nucleotide sugar metabolism.It implies that myo-inositol may improve survival by regulating energy or nutrient absorption.Additionally,the accelerated metamorphosis by myo-inositol may improve survival from megalopa to crablet of S.paramamosain.Overall,this study will provide the first insights into the underlying molecular mechanisms by which myo-inositol increases metamorphosis and survival.

关键词： Myo-inositol Scylla paramamosain Cuticle decomposition and reconstruction Chitin Metamorphosis Survival

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中国北方沿海“褶牡蛎”的分类和订名

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海洋科学 2009年第10期33卷 104-106页

作者：王海艳郭希明刘晓许飞张素萍徐凤山张国范中国科学院海洋研究所山东青岛266071 Haskin shellfish Research laboratory Rutgers University

中国牡蛎的种类繁多,多样性极为丰富,由于其形态变异大,已报道的中国牡蛎物种组成及分布争议甚大,中国北方沿海常见"褶牡蛎"的分类也一直存在很大的争议,这严重阻碍了其他相关研究的进行。作者系统综述了中国北方沿海"褶牡蛎"的分类历... 详细信息

中国牡蛎的种类繁多,多样性极为丰富,由于其形态变异大,已报道的中国牡蛎物种组成及分布争议甚大,中国北方沿海常见"褶牡蛎"的分类也一直存在很大的争议,这严重阻碍了其他相关研究的进行。作者系统综述了中国北方沿海"褶牡蛎"的分类历史和研究现状,并对中国北方沿海"褶牡蛎"进行了系统的分类研究,取得了最新研究成果。确立了中国北方沿海"褶牡蛎"的分类地位,改变其分类长期混乱的局面,这对于"褶牡蛎"的养殖和其他相关研究均具有重要的意义。

关键词： “褶牡蛎” 分类订名

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Impact of seawater carbonate variables on post-larval bivalve calcification

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Journal of Oceanology and Limnology 2018年第2期36卷 405-413页

作者：李加琦毛玉泽蒋增杰张继红卞大鹏方建光 Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao 266071 China laboratory of Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China Function laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China National Engineering and Research Center of Marine shellfish Weihai 264316 China

Several studies have demonstrated that shellfish calcification rate has been impacted by ocean acidification. However, the carbonate system variables responsible for regulating calcification rate are controversial. To distinguish the key variables, we manipulated a seawater carbonate system by regulating seawater pH and dissolved inorganic carbon(DIC). Calcification rates of juvenile blue mussel(M ytilus edulis) and Zhikong scallop(C hlamys farreri) were measured in different carbonate systems. Our results demonstrated that neither [HCO ˉ^+ 3 ], DIC, or p H([H ^+ ]) were determining factors for the shellfish calcification rate of blue mussel or Zhikong scallop. However, a significant correlation was detected between calcification rate and DIC/[H ^+ ] and [CO_3^(2ˉ)] in both species.

关键词： ocean acidification calcification shellfish carbonate system

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Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas:segregation and null allele identification for linkage analysis

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Chinese Journal of Oceanology and Limnology 2009年第1期27卷 74-79页

作者：李莉郭希明张国范 Key laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Haskin shellfish Research laboratory Institute of Coastal and Marine Sciences Rutgers University

Microsatellites were screened in a backcross family of the Pacific oyster, Crassostrea gigas. Fifteen microsatellite loci were distinguishable and polymorphic with 6 types of allele-combinations. Null alleles were detected in 46.7% of loci, accounting for 11.7% of the total alleles. Four loci did not segregate in Mendelian Ratios. Three linkage groups were identified among 7 of the 15 segregating loci. Fluorescence-based automated capillary electrophoresis (ABI 310 Genetic Analyzer) that used to detect the microsatellite loci, has been proved a fast, precise, and reliable method in microsatellite genotyping.

关键词：基因连锁分析太平洋牡蛎微卫星位点鉴定分离微型毛细管电泳 ABI公司

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