High dietary lipid level promotes low salinity adaptation in the marine euryhaline crab(Scylla paramamosain)

作     者：Jiaxiang Luo Chen Ren Tingting Zhu Chen Guo Shichao Xie Yingying Zhang Zheng Yang Wenli Zhao Xiangsheng Zhang Jingjing Lu Lefei Jiao Qicun Zhou Douglas R.Tocher Min Jin Jiaxiang Luo;Chen Ren;Tingting Zhu;Chen Guo;Shichao Xie;Yingying Zhang;Zheng Yang;Wenli Zhao;Xiangsheng Zhang;Jingjing Lu;Lefei Jiao;Qicun Zhou;Douglas R.Tocher;Min Jin

作者机构：Laboratory of Fish and Shellfish NutritionSchool of Marine SciencesNingbo UniversityNingbo 315211China Guangdong Provincial Key Laboratory of Marine BiotechnologyInstitute of Marine SciencesShantou UniversityShantou 515063China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2023年第12卷第1期

页      面：297-307页

核心收录：

学科分类：090801[农学-水产养殖] 0908[农学-水产] 0905[农学-畜牧学] 09[农学] 0906[农学-兽医学] 

基　　金：This study was supported by Nature Science Foundation of Zhejiang Province(LY21C190006) the National Natural Science Foundation of China(32072987). 

主　　题：Osmotic pressure regulation Mitochondrial energy metabolism Lipid metabolism 

摘      要：The physiological processes involved in adaptation to osmotic pressure in euryhaline crustaceans are highly energy demanding,but the effects of dietary lipids(fat)on low salinity adaptations have not been well evaluated.In the present study,a total of 120 mud crabs(Scylla paramamosain,BW=17.87±1.49 g)were fed control and high-fat(HF)diets,at both medium salinity(23‰)and low salinity(4‰)for 6 wk,and each treatment had 3 replicates with each replicate containing 10 crabs.The results indicated that a HF diet significantly mitigated the reduction in survival rate,percent weight gain and feed efficiency induced by low salinity(P0.05).Low salinity lowered lipogenesis and activated lipolysis resulting in lipid depletion in the hepatopancreas of mud crabs(P0.05).Thus,HF diets enhanced the process of lipolysis to supply more energy.In the gills,low salinity and the HF diet increased the levels of mitochondrial biogenesis markers,the activity of mitochondrial complexes,and the expression levels of genes related to energy metabolism(P0.05).Consequently,the positive effects of the HF diet on energy metabolism in mud crabs at low salinity promoted osmotic pressure regulation.Specifically,significantly higher haemolymph osmotic pressure and inorganic ion content,as well as higher osmotic pressure regulatory enzyme activity in gills,and gene and protein expression levels of NaK-ATPase were observed in crabs fed the HF diet at low salinity(P0.05).In summary,high dietary lipid levels improved energy provision to facilitate mitochondrial biogenesis,which increased ATP provision for osmotic pressure regulation of mud crabs.This study also illustrates the importance of dietary lipid nutrition supplementation for low salinity adaptations in mud crabs.