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Cryo-electron microscopy reconstructions of two types of wild rabbit hemorrhagic disease viruses characterized the structural features of Lagovirus

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Protein & Cell 2010年第1期1卷 48-58页

作者： Zhongjun Hu Xiaojuan Tian Yujia Zhai Wei Xu Dong Zheng Fei Sun National laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China Department of Biochemistry and Molecular biology College of Life SciencesBeijing Normal UniversityBeijing 100875China

Rabbit hemorrhagic disease was described in China in 1984 and can cause hemorrhagic necrosis of the liver within two or three days after *** etiological agent,rabbit hemorrhagic disease virus(RHDV),belongs to the Lagovirus genus in the Caliciviridae *** to other calicivirus,such as rNV and SMSV,the structure of Lagovirus members is not well *** this report,structures of two types of wild RHDV particles,the intact virion and the core-like particle(CLP),were reconstructed by cryo-electron microscopy at 11Åand 17Å,*** is the first time the 3D structure of wild caliciviruses CLP has been provided,and the 3D structure of intact RHDV virion is the highest resolution structure in *** of the intact virion and CLP structures clearly indicated that CLP was produced from the intact virion with the protrusion *** contrast with the crystal structures of recombinant Norovirus and San Miguel sea lion virus,the capsomers of RHDV virion exhibited unique structural features and assembly *** P1 and P2 subdomains have interactions inside the AB capsomer,while only P2 subdomains have interaction inside CC *** pseudo atomic models of RHDV capsomers were constructed by homology modeling and density map fitting,and the rotation of RHDV VP60 P domain with respect to its S domain,compared with SMSV,was ***,our cryo-electron microscopic studies of RHDV provide close insight into the structure of Lagovirus,which is important for functional analysis and better vaccine development in the future.

关键词： rabbit hemorrhagic disease virus calicivirus Lagovirus core-like particle cryo-electron microscopy reconstruction

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Elucidating the role of embedding dispersed cobalt sites in nitrogen-doped carbon frameworks in Si-based anodes for stable and superior storage

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Journal of Energy Chemistry 2024年第11期98卷 180-195页

作者： Yueying Chen Ping Li Mianying Huang Chunlei Wu Qianhong Huang Tingyang Xie Xiaoming Lin Akif Zeb Yongbo Wu Zhiguang Xu Yuepeng Cai Joint laboratory of Energy and Materials Chemistry(South China Normal University-Guangzhou Tianci New Materials Co.Ltd.) Key Laboratory of Theoretical Chemistry of EnvironmentMinistry of EducationSchool of ChemistrySouth China Normal UniversityGuangzhou 510006GuangdongChina Department of Chemistry and Chemical Engineering Jiangxi Normal UniversityNanchang 330022JiangxiChina Guangzhou Huifu Research Institute Co.Ltd Nanxiang San LuGuangzhou 510663GuangdongChina Key laboratory of Atomic and Subatomic Structure and Quantum Control(Ministry of Education) Guangdong Basic Research Center of Excellence for Structure and Fundamental Interactions of MatterSchool of PhysicsSouth China Normal UniversityGuangzhou 510006GuangdongChina Guangdong Provincial Key laboratory of Quantum Engineering and Quantum Materials Guangdong-Hong Kong Joint Laboratory of Quantum MatterSouth China Normal UniversityGuangzhou 510006GuangdongChina

Unsatisfactory conductivity and volume effects have hindered the commercial application of siliconbased materials as advanced anode materials for high-performance lithium-ion batteries. Herein, nitrogen doped carbon silicon matrix composite with atomically dispersed Co sites(Si/Co-N-C) is obtained via the design of the frame structure loaded with nano-components and the multi-element hybrid strategy. Co atoms are uniformly fixed to the N-C frame and tightly packed with nanoscale silicon particles as an activation and protection building block. The mechanism of the N-C framework of loaded metal Co in the Si alloying process is revealed by electrochemical kinetic analysis and ex situ characterization ***, the nitrogen-doped Co site activates the intercalation of the outer carbon matrix to supplement the additional capacity. The Co nanoparticles with high conductivity and support enhance the conductivity and structural stability of the composite, accelerating the Li^(+)/Na^(+) diffusion kinetics. Density functional theory(DFT) calculation confirms that the hetero-structure Si/Co-N-C adjusts the electronic structure to obtain good lithium-ion adsorption energy, reduces the Li^(+)/Na^(+) migration energy *** work provides meaningful guidance for the development of high-performance metal/non-metal modified anode materials.

关键词： Co nanoparticles Nitrogen doped carbon Silicon Lithium/sodium storage Metal-organic frameworks(MOFs)

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interactions between bubble and particles of key minerals of diasporic bauxite through the extended DLVO theory

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International Journal of Mining Science and Technology 2022年第1期32卷 201-214页

作者： Ningning Zhang Tian Pang Rui Han Songjiang Chen Zhen Li Yuexian Yu Zhongyu Shi Lijun Liu Jinzhou Qu Anning Zhou College of Chemistry and Chemical Engineering Xi’an University of Science and TechnologyXi’an 710054China Key laboratory of Coal Resources Exploration and Comprehensive Utilization Ministry of Natural Resources of ChinaXi’an 710021China

The flotation of diasporic bauxite is to separate diaspore(valuable mineral)from aluminosilicate minerals(gangue minerals,mainly including kaolinite,illite and pyrophyllite),and the microscopic interaction force between the two types of minerals and air bubbles determines the separation *** this paper,based on the extended Derjaguin-Landau-Verwey-Overbeek(DLVO)theory,the van der Waals,electrostatic and hydrophobic interaction between particles of the four minerals mentioned above and air bubbles in collectorless solution were calculated first,and then diaspore and kaolinite were taken as examples to analyze the influence of various factors such as electrolyte concentration,mineral particle size,air bubble size,collector type(dodecylamine hydrochloride(DAH)and sodium oleate(NaOL))and concentration,and pulp pH on the interactions between the particles of valuable mineral and gangue minerals and air *** results showed that the total extended DLVO interactions between the four minerals and air bubbles were repulsive in most cases in collectorless *** increase in electrolyte concentration reduced the interaction force or even changed the direction of the force under certain *** addition of DAH and NaOL can reduce the adhesion energy barrier of kaolinitebubble and diaspore-bubble *** type of minerals exhibited a specific interface interaction response with air bubbles in each collector with different pH *** research results have theoretical guiding significance for the optimization and directional control of diasporic bauxite flotation conditions.

关键词： Particle-bubble Interaction force Diaspore Aluminosilicate minerals Extended DLVO theory

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Structure of Solution of Colloid and Hydrogen Bonding Fluid Near a Semipermeable Membrane

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Chemical Research in Chinese Universities 2017年第2期33卷 248-254页

作者： ZHAO Meng CHEN Hanfei GU Fang WANG Haijun College of Chemistry and Environmental Science Hebei University Baoding 071002 P. R. China Chemical biology Key laboratory of Hebei Province Hebei University Baoding 071002 P. R. China Key laboratory of Medicinal Chemistry and Molecular Diagnosis Ministry of Education Hebei University Baoding 071002 P. R. China

The local fluid structure of a solution consisting of colloid particles and hydrogen bonding（HB） fluid se-parated from pure solvent by a semipermeable membrane was investigated, where the colloid particles and HB fluidserve as solute and solvent, respectively. In this paper, the semipermeable membrane allows the passage of solventmolecules but not solute particles, and therefore, it provides a nano-confinement for colloid particles. The densityprofiles of the two species near the semipermeable membrane were determined via density functional theory（DFT）for classical fluids. Based on the predicted density profiles under various conditions, the effects of HB strength, func-tionality, total bulk density, density fraction and the size ratio of two species on the fluid structure were discussed. Asan application, the osmotic pressures of the system were also presented. It is shown that the local structure andosmotic pressure can be efficiently regulated by these factors due to the competition between the excluded volumeinteractions and the FIB interaction. The present results are expected to be helpful to study the osmotic phenomenaand relevant problems on the nanoscale.

关键词： Hydrogen bonding fluid Semipermeable membrane Osmotic pressure Density functional theory

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Towards growth of pure AB-stacked bilayer graphene single crystals

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Nano Research 2024年第5期17卷 4616-4621页

作者： Xiaowen Zhang Tao Zhou Yunlong Ren Zuo Feng Ruixi Qiao Qinghe Wang Bin Wang Jinxia Bai Muhong Wu Zhilie Tang Xu Zhou Kaihui Liu Xiaozhi Xu Guangdong Basic Research Center of Excellence for Structure and Fundamental interactions of Matter Guangdong Provincial Key Laboratory of Quantum Engineering and Quantum MaterialsSchool of PhysicsSouth China Normal UniversityGuangzhou 510006China Guangdong-Hong Kong Joint laboratory of Quantum Matter School of PhysicsSouth China Normal UniversityGuangzhou 510006China Physical Science and Engineering Division King Abdullah University of Science and TechnologyThuwal 23955–6900Saudi Arabia State Key laboratory for Mesoscopic Physics Frontiers Science Center for Nano-optoelectronicsSchool of PhysicsPeking UniversityBeijing 100871China Institute for Frontier Science Nanjing University of Aeronautics and AstronauticsNanjing 210094China International Centre for Quantum Materials Collaborative Innovation Centre of Quantum MatterPeking UniversityBeijing 100871China Songshan Lake Materials laboratory Institute of PhysicsChinese Academy of SciencesDongguan 523808China

Given its intriguing band structure and unique tunable bandgap,AB-stacked bilayer graphene has great potentials in the applications of high-end electronics,optoelectronics and *** epitaxial growth of AB-stacked single-crystal bilayer graphene films requires a strict AB-stacked lattice,identical orientations and seamless stitching of bilayer graphene ***,the particles inevitably present on the metal surface that produced during high temperature growth would induce random orientations,twisted stacking islands,and uncontrollable multilayers,which is a great challenge to ***,we propose a heat-resisting-box assisted strategy to produce nearly pure AB-stacked bilayer graphene single-crystal films on Cu/Ni(111)*** our technique,the particles on the Cu/Ni(111)surface are effectively eliminated,which greatly minimizes the occurrence of randomly twisted islands and uncontrollable *** as-grown AB-stacked bilayer graphene films show>99%alignment and>99%AB stacking *** work provides a promising method towards the growth of pure AB-stacked bilayer graphene single crystals and would accelerate its device applications.

关键词： bilayer graphene AB stacking uniform growth heat-resisting box

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A reflective display based on the electro-microfluidic assembly of particles within suppressed water-in-oil droplet array

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Light(Science & Applications) 2023年第12期12卷 2742-2754页

作者： Shitao Shen Haoqiang Feng Yueming Deng Shuting Xie Zichuan Yi Mingliang Jin Guofu Zhou Paul Mulvaney Lingling Shui Guangdong Basic Research Center of Excellence for Structure and Fundamental interactions of Matter Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and DevicesSchool of Information and Optoelectronic Science and EngineeringSouth China Normal University510006 GuangzhouChina International Joint laboratory of Optofluidic Technology and System(LOTS) National Center for International Research on Green OptoelectronicsSouth China Academy of Advanced OptoelectronicsSouth China Normal University510006 GuangzhouPeople’s Republic of China School of Electronic Information University of Electronic Science and Technology of ChinaZhongshan Institute528402 ZhongshanChina ARC Centre of Excellence in Exciton Science School of ChemistryUniversity of MelbourneParkvilleVIC 3010Australia

Reflective displays have stimulated considerable interest because of their friendly readability and low energy ***,we develop a reflective display technique via an electro-microfluidic assembly of particles(eMAP)strategy whereby colored particles assemble into annular and planar structures inside a dyed water droplet to create"open"and"closed"states of a display ***-in-oil droplets are compressed within microwells to form a pixel *** particles dispersed in droplets are driven by deformation-strengthened dielectrophoretic force to achieve fast and reversible motion and assemble into multiple *** eMAP based device can display designed information in three primary colors with≥170°viewing angle,~0.14 s switching time,and bistability with an optimized material *** proposed technique demonstrates the basis of a high-performance and energy-saving reflective display,and the display speed and color quality could be further improved by structure and material optimization;exhibiting a potential reflective display technology.

关键词： deformation reflective fluid

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Light scattering based analyses of the effects of bovine serum proteins on interactions of magnetite spherical particles with cells

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Chinese Chemical Letters 2018年第8期29卷 1291-1295页

作者： Lingling Guo Ting Wang Zhan Chen Nongyue He Yaozhong Chen Tao Yuan State Key laboratory of Bioelectronics National Demonstration Center for Experimental Biomedical Engineering Education School of Biological Science and Medical Engineering Southeast University Department of Chemistry University of Michigan Department of Orthopedic NanjingJinling Hospital

Information concerns endocytosis and autophagic effects of magnetite particles is crucial for understanding the particle-cell interactions. In this work, we investigated the effects of bovine serum proteins on the endocytosis of magnetite spherical particles（MSPs）. Autophagic effects of MSPs in breast cancer cells were studied. Light scattering based flow cytometry and microscopy were used for evaluating the uptake potential of MSPs by cells and the cellular autophagosome accumulation. Results showed bovine serum proteins significantly reduced the endocytosis of MSPs by decreasing their adsorption to cell membranes. Additionally, serum proteins had influences on the endocytic mechanisms of *** accumulation could be caused by the internalized MSPs rather than the particles associated with cell membrane. Above fundamental findings promote our understandings upon the interactions of MSPs with cells. Light scattering based methods were proved to be simple and *** present work may promote their application in studies upon endocytosis of metallic particles in the future.

关键词： Light scattering Endocytosis Autophagic effects Autophagosome Magnetite spherical particles

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Pd nanoparticles immobilized on graphite oxide modified with a base: Highly efficient catalysts for selective hydrogenation of citral

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Science China Chemistry 2013年第2期56卷 203-209页

作者： ZHAO YanFei ZHANG HongYe HUANG ChangLiang CHEN Sha YU Bo XU JiLei LIU ZhiMin Beijing National laboratory for Molecular Sciences Key Laboratory of Colloid Interface and Thermodynamics Institute of Chemistry Chinese Academy of Sciences

In this work, the Pd-based catalysts were designed via immobilizing Pd nanoparticles on graphite oxide (GO) modified with organic base, 1,1,3,3-tetramethylguanidine (TMG), which was used for the selective hydrogenation of citral. These catalysts were characterized by various techniques including IR, X-ray diffraction, transmission electron microscopy, and X-ray photoelectron spectroscopy. It was demonstrated that the Pd particles with size less than 5 nm were uniformly distributed throughout the support, and they were in the electron-deficient state due to the strong interactions with the modified support. The resultant Pd-TMG/GO catalyst displayed high efficiency for the selective hydrogenation of citral with a turnover frequency of 7100 h-1 as well as superior selectivity to citronellal of 89.6%. Moreover, the catalyst can be reused for five times without obvious activity loss, which may result from its stable structure.

关键词： tetramethylguanidine graphite oxide palladium citral hydrogenation

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Structural Evolution During Mechanical Milling of Bimodal-Sized Al_2O_3 particles Reinforced Aluminum Matrix Composite

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Acta Metallurgica Sinica(English Letters) 2018年第4期31卷 423-430页

作者： Ke Zhao Dan Tang Jin-Ling Liu Yi-Guang Wang Science and Technology on Thermostructural Composite Materials laboratory Northwestern Polytechnical University State Key laboratory of Traction Power School of Mechanics and Engineering Southwest Jiaotong University Applied Mechanics and Structure Safety Key laboratory of Sichuan Province Southwest Jiaotong University

Hybrid aluminum matrix composite powders reinforced with bimodal-sized Al2O3 particles were synthesized by mechanical *** different approaches were investigated for the addition of submicron-and nano-sized Al2O3 particles to the aluminum powders,It was observed that the simultaneous addition of bimodal-sized Al2O3 particles to the aluminum powders resulted in an equiaxed morphology of the composite powders and the average particle size stabilized after 5 h of milling,indicating that the presence of bimodal-sized particles has greater effect on accelerating milling process as compared to nano-sized particles;the grain size of the aluminum matrix in composite powders was reduced to under 40 nm,approximate to the value obtained in the separate addition case,while a lower rate of refining was observed due to hindrance of submicron-sized particles on the interactions between nano-sized particles and the aluminum matrix.

关键词： Bimodal-sized Aluminum matrix composite Mechanical milling Grain refinement

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Influence of ship emissions on NOx, SO2, O3 and PM concentrations in a North-Sea harbor in France

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Journal of Environmental Sciences 2018年第9期30卷 56-66页

作者： Frederic Ledoux Cloe Rochet Fabrice Cazier Charles Beaugard Dominique Courcot Unit of Environmental Chemistry and interactions with Life UCEIV EA4492 SFR Condorcet FR CNRS 3417 University of the Littoral OpalCoast F-59140 Dunlterque France Centre Commun de Mesures University of the Littoral Opal Coast F-59140 Dunkerque France Atmo Hauts-de-France F-59800 Lille France

The influence of in-port ship emissions on gases and PM10 concentrations has been estimated in the port city of Calais, northern France, one of the busiest harbor in Europe, with numerous rotations of ferries or roll-on/roll-off cargo in average per day. NOx, SO2, O3 and PM10 concentrations were continuously measured over a three-month period, as well as real-time particle size distribution. A rural site located at Cape Gris-Nez, 20 km from Calais, was considered to deduce intrinsic contribution of ship emissions at the harbor city. The average concentrations of the studied species as well as the pattern of the conditional bivariate probability function at the two sites evidenced that in-port shipping, especially during the maneuvering operations, has an important influence on the NOx and SO2 concentrations. The impact of shipping in the harbor of Calais on average concentrations was estimated to 5l% for SO2, 35% for NO, 15% for NO2 and 2% for PM10 in the studied period. Concentration peaks of SO2 and NOx associated with an 03 depletion appeared synchronized with departures and arrivals of ferries. For winds blowing from the harbor, when compared to the background level, the number of particles appeared 10 times higher, with the highest differences in the 30-67 nm and the 109-167 nm size ranges. The average impact of in-port ships on PM10 concentrations was estimated to ＋28.9 μg/m^3 and concerned mainly the PM1 size fraction （40%）. Punctually, PM10 can potentially reach a concentration value close to 100μg/m^3.

关键词： Ships Emissions Harbor Gases particles Air pollution

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