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A finTRIM member 100 (FTR100) is unique to Otomorpha fish for constitutive regulation of IFN response

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Water Biology and Security 2023年第2期2卷 80-90页

作者： Wen-Hao Guo Cheng Dan Xiu-Ying Gong Yi-Lin Li Hao-Yu Sun Zi-Ling Qu Li-Li An Xiang Zhao Jie Mei Jian-Fang Gui Yi-Bing Zhang State Key laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan430072China University of Chinese Academy of Sciences Beijing10049China College of Fisheries Huazhong Agricultural UniversityWuhan430070China The Innovation Academy of Seed Design Chinese Academy of SciencesWuhan430072China

Vertebrate interferon(IFN)expression is fine-tuned in order to avoid excessive tissue injury under normal conditions and during virus ***(fish novel TRIM,FTR)proteins are reported to regulate the fish IFN ***,we identify a novel finTRIM gene from yellow catfish(Pelteobagrus fulvidraco),which is sequentially named PfFTR100 according to the nomenclature rule in ***-wide analyses reveal that FTR100 is unique to Otomorpha fish,with a single copy in spite of additional genome duplication in some fish *** that few of the 99 finTRIM genes identified in zebrafish are conserved in main fish branches and most,such as FTR100,are unique to distinct branches due to lineage-specific expansion of finTRIM genes,we develop a nomenclature for newly cloned finTRIM genes from different fish ***100 mRNA is not induced by virus infection,with a relatively high expression level comparable to that of cellular IFN and some IFN-stimulated genes(ISGs)in virally-infected ***,ectopically-expressed PfFTR100 protein is attenuated in virally-infected cells through the proteasomal-dependent *** of PfFTR100 promotes SVCV replication by downregulating the constitutive and inducible IFN response via a mechanism by which PfFTR100 targets IRF3 and IRF7 to attenuate their mRNA levels rather than their protein *** results indicate that yellow catfish FTR100 is essential for homeostatic regulation of fish tonic IFN response.

关键词： Constitutive expression FTR100 Homeostatic regulation Lineage-specific expansion Tonic IFN response

The ghrelinergic system in zebrafish gonads is suppressed during food unavailability

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Aquaculture and Fisheries 2022年第5期7卷 494-499页

作者： Azadeh Hatef Jithine Jayakumar Rajeswari Suraj Unniappan laboratory of Integrative Neuroendocrinology Department of Veterinary Biomedical SciencesWestern College of Veterinary MedicineUniversity of SaskatchewanSaskatoonSaskatchewanS7N 5B4Canada Toxicology Centre University of SaskatchewanSaskatoonSaskatchewanS7N 5B3Canada

Ghrelin is an appetite stimulatory peptide that regulates reproduction in *** acylation of ghrelin by ghrelin-O-acyltransferase(Goat)is essential for most of its *** ghrelinergic system(ghrelin,ghrelin receptor[Ghsr]and Goat)is present in teleost fish ovary and *** this research,we determined the abundance of the ghrelinergic system mRNAs in different stages of follicle(oocyte)development in zebrafish *** highest levels of preproghrelin mRNA were observed in the first stage of follicular development(previtellogenesis).Significantly low levels of preproghrelin mRNA were observed in stages 3II,4 and 5 of the follicle *** mRNA abundance remained unchanged at early stages of follicle development and decreased in stage 5 compared to other developmental stages.A significant decrease in preproghrelin and Ghsr mRNAs in the testis,and ghsr mRNA in the ovary of zebrafish deprived of food for 3 days were *** 7 days food deprivation,preproghrelin,ghsr and goat mRNA in the ovary and preproghrelin and goat in the testis of zebrafish were significantly *** results show that metabolic status modulates gonadal ghrelinergic system in zebrafish and provide support for a role for ghrelin in fish reproduction.

关键词： Ghrelin Ghrelin receptor Ghrelin-O-acyltransferase Fasting Ovary Testis Follicle zebrafish

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HSF4调控RAD51介导DNA损伤修复

HSF4调控RAD51介导DNA损伤修复

A 14-AA Peptide Derived from CLV3 is Sufficient to Regulate the Stem Cell Population in Arabidopsis

遗传资源的保护和开发利用——2012年中国青年遗传学家论坛

作者： Stephen Archacki David Wan-Cheng Li Key laboratory of Molecular Biophysics of Ministry of Education College of Life Science and TechnologyCenter for Human Genome ResearchHuazhong University of Science and TechnologyWuhanHubei 430074P.R.China

Heat shock factor protein 4(HSF4)is expressed exclusively in the ocular lens and plays a critical role in the lens formation and *** in the HSF4 gene lead to congenital and senile ***,the molecular mechanisms causing this disease have not been well *** damage in lens is a crucial risk factor in senile cataract formation,and its timely repair is essential for maintaining the lens’ *** study firstly found evidence that HSF4 contributes to the repair of DNA strand ***,this does not occur with cataract causative mutations in *** verify that DNA damage repair is mediated by the binding of HSF4 to a heat shock element in the Rad51 promoter,a gene which assists in the homologous recombination(HR)repair of DNA strand ***4 up-regulates Rad51 expression while mutations in HSF4 fail,and DNA does not get repaired. Camptothecin,which interrupts the regulation of Rad51 by HSF4,also affects DNA damage ***,with HSF4 knockdown in the lens of zebrafish,DNA damage was observed and the protein level of Rad51 was significantly *** study presents the first evidence demonstrating that HSF4 plays a role in DNA damage repair and may contribute a better understanding of congenital cataract formation.

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A 14-AA Peptide Derived from CLV3 is Sufficient to Regulate ...

Comparative Transcriptome Analysis Identifies Candidate Genes Related to Skin Color Differentiation in Red Tilapia

The Second International Symposium on Plant neurobiology

作者： Martijn Fiers Plant research International Wageningen The Netherlands Center for Signal Transduction ＆ Metabolomics Institute of Botany

＜正＞Stem cells positioned in the central zone of the plant shoot apical meristem （SAM） are the source of totipotent cells, which give rise continuously to new organs post-embryonically. These slow-dividing cells maintain simultaneously two antagonistic events, cell proliferation and cell differentiation in a similar manner to animal stem cells. The determination of the fate of the progeny cells is made by a

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Comparative Transcriptome Analysis Identifies Candidate Gene...

The ERα/KDM6B regulatory axis modulates osteogenic differentiation in human mesenchymal stem cells

江苏省遗传学会2016年学术年会

作者：朱文彬 Lanmei Wang 董在杰 Shanghai Ocean Univercity Freshwater Fisheries research Center of Chinese Academy of Fishery Sciences

Red tilapia is becoming more popular for aquaculture production in China in recent ***,the pigmentation differentiation in genetic breeding is the main problem limiting its development of commercial red tilapia culture and the genetic basis of skin color variation is still *** this study,we conducted Illumina sequencing of transcriptome on three color variety red tilapia.A total of 224,895,758 reads were generated,resulting in 160,762 assembled contigs that were used as reference *** contigs of red tilapia transcriptome had hits in the range of 53.4% to 86.7% of the unique proteins of zebrafish,fugu,medaka,three-spined stickleback and *** 44,723 contigs containing 77,423 simple sequence repeats（SSRs） were identified,with 16,646 contigs containing more than one *** skin transcriptomes were compared pairwise and the results revealed that there were 148 common significantly differentially expressed unigenes and several key genes related to pigment synthesis,***,tyrp1,silv,sox10,slc24 a5,cbs and slc7 a11,were *** results will facilitate understanding the molecular mechanisms of skin pigmentation differentiation in red tilapia and accelerate the molecular selection of the specific strain with consistent skin colors.

关键词： Red tilapia Transcriptome Skin Color Candidate Genes

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Bone research 2022年第1期10卷 95-106页

作者： Zhenqing Liu Hye-Lim Lee Jin Sook Suh Peng Deng Chang-Ryul Lee Olga Bezouglaia Mojan Mirnia Vivian Chen Michael Zhou Zhong-Kai Cui Reuben HKim Min Lee Tara Aghaloo Christine Hong Cun-Yu Wang Division of Oral Biology and Medicine School of DentistryUniversity of CaliforniaLos Angeles(UCLA)Los AngelesCA 90095USA Department of Anatomy&neurobiology University of CaliforniaIrvine(UCI)IrvineCA 92697USA Department of Orofacial Sciences School of DentistryUniversity of CaliforniaSan Francisco(UCSF)San FranciscoCA 94143USA School of Dentistry University of CaliforniaSan Francisco(UCSF)San FranciscoCA 94143USA Division of Diagnostic and Surgical Sciences School of DentistryUniversity of CaliforniaLos Angeles(UCLA)Los AngelesCA 90095USA School of Dentistry University of CaliforniaLos Angeles(UCLA)Los AngelesCA 90095USA Division of Advanced Prosthodontics School of DentistryUniversity of CaliforniaLos Angeles(UCLA)Los AngelesCA 90095USA Division of Constitutive and Regenerative Sciences School of DentistryUniversity of CaliforniaLos Angeles(UCLA)Los AngelesCA 90095USA Department of Bioengineering University of CaliforniaLos Angeles(UCLA)Los AngelesCA 90095USA

Osteoporosis is a highly prevalent public health burden associated with an increased risk of bone fracture, particularly in aging women. Estrogen, an important medicinal component for the preventative and therapeutic treatment of postmenopausal osteoporosis, induces osteogenesis by activating the estrogen receptor signaling pathway and upregulating the expression of osteogenic genes, such as bone morphogenetic proteins(BMPs). The epigenetic regulation of estrogen-mediated osteogenesis,however, is still unclear. In this report, we found that estrogen significantly induced the expression of lysine-specific demethylase 6B(KDM6B) and that KDM6B depletion by shRNAs led to a significant reduction in the osteogenic potential of ***, upon estrogen stimulation, estrogen receptor-α(ERα) was recruited to the KDM6B promoter, directly enhancing KDM6B expression. Subsequently, KDM6B was recruited to the BMP2 and HOXC6 promoters, resulting in the removal of H3K27me3 marks and activating the transcription of BMP2 and HOXC6, the master genes of osteogenic differentiation. Furthermore, we found that estrogen enhanced DMSC osteogenesis during calvarial bone regeneration and that estrogen’s pro-osteogenic effect was dependent on KDM6B in vivo. Taken together, our results demonstrate the vital role of the ERα/KDM6B regulatory axis in the epigenetic regulation of the estrogen-dependent osteogenic response.

关键词： expression stimulation ERα

In vivo imaging reveals mature Oligodendrocyte division in adult zebrafish

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Cell Regeneration 2021年第1期10卷 188-200页

作者： Suqi Zou Bing Hu Institute of Life Science Nanchang UniversityNanchangJiangxi 330031P.R.China School of Life Sciences Nanchang UniversityNanchangJiangxi 330031P.R.China Hefei National laboratory for Physical Sciences at the Microscale University of Science and Technology of ChinaHefeiAnhui 230026P.R.China

Whether mature oligodendrocytes(mOLs)participate in remyelination has been disputed for several ***,some studies have shown that mOLs participate in remyelination by producing new ***,whether mOLs can produce new oligodendrocytes by asymmetric division has not been *** is a perfect model to research remyelination compared to other *** this study,optic nerve crushing did not induce local mOLs *** optic nerve transplantation from olig2:eGFP fish to AB/WT fish,olig2^(+)cells from the donor settled and rewrapped axons in the *** identifying these rewrapping olig2^(+)cells as mOLs at 3 months posttransplantation,in vivo imaging showed that olig2^(+)cells ***,in vivo imaging of new olig2^(+)cell division from mOLs was also captured within the ***,fine visual function was renewed after the remyelination program was *** conclusion,our in vivo imaging results showed that new olig2^(+)cells were born from mOLs by asymmetric division in adult zebrafish,which highlights the role of mOLs in the progression of remyelination in the mammalian CNS.

关键词： Remyelination Mature oligodendrocyte zebrafish In vivo imaging Asymmetric division

A Deep Learning Approach for Detecting Colorectal Cancer via Raman Spectra

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Biomedical Engineering Frontiers 2022年第1期3卷 149-158页

作者： Zheng Cao Xiang Pan Hongyun Yu Shiyuan Hua Da Wang Danny Z.Chen Min Zhou Jian Wu RealDoctor AI research Center College of Computer Science and TechnologyZhejiang UniversityChina Key laboratory of Cancer Prevention and Intervention Ministry of EducationThe Second Affiliated HospitalZhejiang University School of MedicineChina Institute of Translational Medicine and the Cancer Institute of the Second Affiliated Hospital Zhejiang University School of MedicineChina Department of Computer Science and Engineering University of Notre DameUSA Second Affiliated Hospital School of Medicine and School of Public HealthZhejiang UniversityChina

Objective and Impact *** tumors from normal tissues is vital in the intraoperative diagnosis and pathological *** this work,we propose to utilize Raman spectroscopy as a novel modality in surgery to detect colorectal cancer *** spectra can reflect the substance components of the target ***,the feature peak is slight and hard to detect due to environmental *** a high-quality Raman spectroscopy dataset and developing effective deep learning detection methods are possibly viable ***,we collect a large Raman spectroscopy dataset from 26 colorectal cancer patients with the Raman shift ranging from 385 to 1545 cm^(−1).Second,a one-dimensional residual convolutional neural network(1D-ResNet)architecture is designed to classify the tumor tissues of colorectal ***,we visualize and interpret the fingerprint peaks found by our deep learning *** results show that our deep learning method achieves 98.5%accuracy in the detection of colorectal cancer and outperforms traditional ***,Raman spectra are a novel modality for clinical detection of colorectal *** proposed ensemble 1D-ResNet could effectively classify the Raman spectra obtained from colorectal tumor tissues or normal tissues.

关键词： colorectal viable modality

Anatomical distributional defects in mutant genes associated with dominant intermediate Charcot-Marie-Tooth disease type C in an adenovirus-mediated mouse model

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Neural Regeneration research 2017年第3期12卷 486-492页

作者： Seo Jin Lee Sandesh Panthi Hyun Woo Jo Jaeyoung Cho Min-Sik Kim Na Young Jeong In Ok Song Junyang Jung Youngbuhm Huh Department of Biomedical Science Graduate SchoolKyung Hee UniversityDongdaemun-guSeoulKorea Department of Medicine Graduate SchoolKyung Hee UniversityDongdaemun-guSeoulKorea Department of Applied Chemistry College of Applied ScienceKyung Hee UniversityYongin-siGyeonggi-doKorea Department of Anatomy and Cell Biology College of MedicineDong-A UniversitySeo-guBusanKorea Department of Reproductive Endocrinology and Infertility Department of Obstetrics and GynecologyCheil General HospitalDankook University College of MedicineJung-guSeoulKorea Department of Anatomy and neurobiology College of MedicineKyung Hee UniversityDongdaemun-guSeoulKorea

Dominant intermediate Charcot-Marie-Tooth disease type C（DI-CMTC） is a dominantly inherited neuropathy that has been classified primarily based on motor conduction velocity tests but is now known to involve axonal and demyelination ***-CMTC is linked to tyrosyl-t RNA synthetase（YARS）-associated neuropathies,which are caused by E196 K and G41 R missense mutations and a single de novo deletion（153-156 del VKQV）.It is well-established that these YARS mutations induce neuronal dysfunction,morphological symptoms involving axonal degeneration,and impaired motor *** present study is the first to describe a novel mouse model of YARS-mutation-induced neuropathy involving a neuron-specific promoter with a deleted mitochondrial targeting sequence that inhibits the expression of YARS protein in the *** adenovirus vector system and in vivo techniques were utilized to express YARS fusion proteins with a Flag-tag in the spinal cord,peripheral axons,and dorsal root *** transfection of YARS-expressing viruses,the distributions of wild-type（WT） YARS and E196 K mutant proteins were compared in all expressed regions; G41 R was not *** proportion of Flag/green fluorescent protein（GFP） double-positive signaling in the E196 K mutant-type mice did not significantly differ from that of WT mice in dorsal root ganglion *** adenovirus genes,and even the empty vector without the YARS gene,exhibited GFP-positive signaling in the ventral horn of the spinal cord because GFP in an adenovirus vector is driven by a cytomegalovirus *** present study demonstrated that anatomical differences in tissue can lead to dissimilar expressions of YARS ***,use of this novel animal model will provide data regarding distributional defects between mutant and WT genes in neurons,the DICMTC phenotype,and potential treatment approaches for this disease.

关键词： nerve regeneration tyrosyl-tRNA synthetase YARS-associated neuropathy YARS mutation Charcot-Marie- Tooth Disease adenoviral vector-mediated mouse models neural regeneration