检索结果-南通市图书馆

Electron transfer in protein modifications:from detection to imaging

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2023年第2期66卷 388-405页

作者： Lei Zhang Mengge Chu Cailing Ji Wenjie Wang Jie Tan Quan Yuan molecular Science and biomedicine laboratory(MBL) State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringHunan UniversityChangsha410082China

Signal pathways participate in vital biological processes and regulate complex life activities through protein modifications.Protein modifications in signal pathways are accompanied by electron transfer.The study of electronic behavior helps to explore the physical and chemical processes in signal pathways,receiving extensive attention.There are some excellent reviews that have summarized methods for signal pathway detection,while few discussions are from an electron transfer perspective.This review describes the relationship between signal pathways and electron transfer in protein modification.Subsequently,we summarize the electron transfer-based detection methods,such as electrochemical,photoelectrochemical and electrochemiluminescence methods.Additionally,the applications of signal pathway detection in mechanism study and imaging are also reviewed.Finally,a comprehensive discussion of the summary and outlooks in this field is presented,aiming to provide valuable guidance for the molecular mechanism of life processes and the development of new analytical techniques.

关键词： protein modification electron transfer signal pathway mechanism imaging

molecular Engineering of Novel Fluorophores for High-Contrast Bioimaging

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2022年第9期40卷 1073-1082页

作者： Shuai Xu Wenjing Pan Tian-Bing Ren Shuang-Yan Huan Lin Yuan Xiao-Bing Zhang molecular Science and biomedicine laboratory State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringCollaborative Innovation Center for Chemistry and Molecular MedicineHunan UniversityChangshaHunan 410082China

Fluorophores play a significant role in achieving high-contrast imaging in complex physiological environment.Herein,we present the results of our studies on the development of various fluorophores,such as anti-solvatochromic fluorophores,long wavelength emission two-photon fluorophores,large Stokes shift fluorophores and solid-state fluorophores,to achieve high-contrast bioimaging in physiological environments.in this study,we have primarily focused on the design,optical performance,imaging mechanisms,and the applications of these fluorophores with respect to complex physiological conditions.

关键词： Fluorescent probes Dyes Sensors Bioimaging molecular engineering

Two birds with one stone:Amine-functionalized MSNs@Eu(OH)CO_(3) nanoprobe for efficient dissolution-enhanced afterglow bioassay

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2022年第9期15卷 8360-8366页

作者： Fuying Zhang Donghao Hu Xianlong Su Zhengdao Hong Wei Feng Ming Xu Fuyou Li Department of Chemistry&State Key laboratory of molecular Engineering of Polymers&Institute of biomedicine Science Fudan UniversityShanghai 200433China

ABSTRACT The efficient detection of tumor markers is crucial to early cancer diagnosis and monitoring.Here,we propose a unique“two birds with one stone”dissolution-enhanced afterglow bioassay based on amino-functionalized mesoporous SiO2 nanoparticles(MSNs-NH2)encapsulated Eu(OH)CO_(3)nanoprobes.These nanoprobes(i)stably conjugate to the antibody and(ii)are highly soluble in the acidic enhancer solution,and provide a high Eu3+concentration.The released Eu3+ions further activate the photochemical afterglow system to determine the alpha-fetoprotein(AFP)levels in clinical serum samples.Without the use of a real-time excitation source,the interference of autofluorescence and scattering from blood can be avoided.The demonstrated limit of detection(LOD)for AFP was as low as 0.12 ng·mL^(−1).This covalently connected dissolution-enhanced luminescent bioassay(DELBA)and novel afterglow system shows tremendous potential for ultrasensitive and rapid clinical diagnosis.

关键词： bioassay photochemical afterglow mesoporous SiO2 nanoparticles(MSNs) nanocomposites dissolution-enhanced

Photoactivatable Cell-Surface DNAzyme Sensor for Real-Time Monitoring of the Metal Ion-Induced AβAggregation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

CCS Chemistry 2023年第9期5卷 1999-2011页

作者： Lu Liu Mei Zhang Qiming Rong Mengyi Xiong Huang Su Xuhua Zhao Guoliang Ke Xiao-Bing Zhang molecular Science and biomedicine laboratory State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringCollaborative Innovation Center for Chemistry and Molecular MedicineHunan UniversityChangsha 410082 Department of Biochemistry and molecular Biology Shanxi Medical UniversityTaiyuan 030001

Precisely monitoring the distribution and local fluctuation of metal ions,which play vital roles in many physiological and pathological processes,contributes largely to the study of metal ion-induced pathogenesis.However,current reactive fluorescent sensors can hardly realize the monitoring of metal ions in situ due to the premature activation and aimless dispersion of the sensors,which hinder their applications in biological samples.Herein,we report a photoactivatable cell-surface DNAzyme sensor to monitor metal ions in the microenvironment of living cells or tissues with high spatial resolution and temporal control.The sensor can efficiently anchor to the cell membrane through amphipathic interaction,and the activity of the sensor can be restored after the irradiation by light,which avoids false positive signals from the reaction with metal cofactors during delivery.First,the properties of the sensor were investigated either in buffer or on amodel cell surface,inwhich the sensor responded to Zn2+with superior temporal and spatial resolution under light activation.Then,the sensor was applied tomonitor the dynamic changes ofmetal ions during the formation of Aβaggregates in the cellular microenvironment of brain cells and brain tissues.This strategy provides a potential tool to understand the roles ofmetal ions in the progression of diseases,such as Alzheimer’s disease.

关键词： DNAzyme photoactivatable controllability cell-surface Aβaggregates brain tissues

Dynamics and biological relevance of epigenetic N^(6)-methyladenine DNA modification in eukaryotic cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2022年第5期33卷 2253-2258页

作者： Yuwei Sheng Meijuan Zhou Changjun You Xiaoxia Dai State Key laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringMolecular Science and Biomedicine LaboratoryHunan Provincial Key Laboratory of Biomacromolecular Chemical BiologyHunan UniversityChangsha 410082China

DNA methylation represents a major type of DNA modifications that play key roles in diverse biological processes.With the recent development of highly selective and sensitive bioanalytical techniques,N^(6)-methyladenine(6mA)has been characterized as an important internal DNA modification dynamically occurring in multiple eukaryotes including humans.Increasing evidence has indicated that 6mA may act as a novel epigenetic modification involved in regulation of development,stress response and diseases such as cancer and neurodegenerative disorders.We review herein the recent advances in the detection and functional studies of 6mA modification,with special emphasis on its biological consequences and human health relevance as well as its dynamic regulation by various types of methyltransferases,demethylases and 6mA-binding proteins.It can be envisaged that further chemical and biological studies of 6mA modification will lead to a better understanding about its potentially important roles in normal and pathological biological processes.

关键词： DNA modification N^(6)-Methyladenine Epigenetic mark Methyltransferase Demethylase

A review of the safety and efficacy of current COVID-19 vaccines

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Frontiers of Medicine 2022年第1期16卷 39-55页

作者： Zehong Huang Yingying Su Tianying Zhang Ningshao Xia State Key laboratory of molecular Vaccinology and molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious DiseasesStrait Collaborative Innovation Center of Biomedicine and PharmaceuticsSchool of Public HealthXiamen UniversityXiamen 361102China

Vaccination is the most effective and feasible way to contain the coronavirus disease 2019(COVID-19)pandemic.The rapid development of effective COVID-19 vaccines is an extraordinary achievement.This study reviewed the efficacy/effectiveness,immunogenicity,and safety profile of the 12 most progressed COVID-19 vaccines and discussed the challenges and prospects of the vaccine-based approaches in a global crisis.Overall,most of the current vaccines have shown safety and efficacy/effectiveness during actual clinical trials or in the real-world studies,indicating a development of pandemic control.However,many challenges are faced by pandemic control in terms of maximizing the effect of vaccines,such as rapid vaccine coverage,strategies to address variants with immune escape capability,and surveillance of vaccine safety in the medium-and long-terms.

关键词： COVID-19 SARS-CoV-2 vaccine safety efficacy effectiveness immunogenicity

Next-generation sequencing-based analysis of the effect of N^(6)-methyldeoxyadenosine modification on DNA replication in human cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2022年第4期33卷 2077-2080页

作者： Juan Wang Yuwei Sheng Ying Yang Xiaoxia Dai Changjun You molecular Science and biomedicine laboratory State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringHunan Provincial Key Laboratory of Biomacromolecular Chemical BiologyHunan UniversityChangsha 410082China

N^(6)-methyldeoxyadenosine(6 mdA) modification is considered as a new epigenetic mark that may play important roles in various biological processes.However,it remains unclear about the effect of 6 mdA on DNA replication in human cells.Herein,we combined next-generation sequencing with shuttle vector technology to explore how 6 mdA affects the efficiency and accuracy of DNA replication in human cells.Our results showed that 6 mdA neither blocked DNA replication nor induced mutations in human cells.Moreover,we found that the depletion of translesion synthesis DNA polymerase(Pol) κ,Pol η,Pol ι or Pol ζ did not significantly change the biological consequences of 6 mdA during replication in human cells.The negligible impact of 6 mdA on DNA replication is consistent with its potential role in epigenetic gene expression.

关键词： N^(6)-methyldeoxyadenosine DNA replication Next-generation sequencing Shuttle vector technology Translesion synthesis DNA polymerase

Current Advances in Aptamer-based Biomolecular Recognition and Biological Process Regulation

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2022年第4期38卷 847-855页

作者： CHEN Sisi ZHANG Lei YUAN Quan TAN Jie molecular Science and biomedicine laboratory(MBL) Institute of Chemical Biology and Nanomedicine(ICBN)State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringHunan UniversityChangsha410082P.R.China

The interaction between biomolecules with their target ligands plays a great role in regulating biological functions.Aptamers are short oligonucleotide sequences that can specifically recognize target biomolecules via structural complementarity and thus regulate related biological functions.In the past ten years,aptamers have made great progress in target biomolecule recognition,becoming a powerful tool to regulate biological functions.At present,there are many reviews on aptamers applied in biomolecular recognition,but few reviews pay attention to aptamer-based regulation of biological functions.Here,we summarize the approaches to enhancing aptamer affinity and the advancements of aptamers in regulating enzymatic activity,cellular immunity and cellular behaviors.Furthermore,this review discusses the challenges and future perspectives of aptamers in target recognition and biological functions regulation,aiming to provide some promising ideas for future regulation of biomolecular functions in a complex biological environment.

关键词： functions. complementarity enzymatic

Real-Time Monitoring of Dynamic Chemical Processes in Microbial Metabolism with Optical Sensors

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2023年第15期41卷 1836-1840页

作者： Na Chen Di Cheng Tianpei He Quan Yuan Institute of molecular Medicine Renmin Hospital of Wuhan UniversityCollge of Chemistry and Molecular SciencesWuhan UniversityWuhanHubei 430072China molecular Science and biomedicine laboratory(MBL) State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringHunan UniversityChangshaHunan 410082China

Monitoring microbial metabolism is vital for revealing the mechanism of disease related to microbial metabolism and providing guidance for biomanufacturing processes optimization.However,it remains a grand challenge to offer real-time insights into microbial metabolism owing to the complex and dynamic process.In this paper,the recent advances and prospects of optical biosensors including the organic,genetic coding and inorganic optical biosensors are briefly described for real-time monitoring of dynamic microbial metabolism.This paper points out that challenges remain in microbial heterogeneity.We believe that this work will inspire the application of developing new methods for single cell real-time analysis.

关键词： Real-time Microbial metabolism Dynamic Monitoring Optical biosensor