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interaction between early in ovo stimulation of the gut microbiota and chicken host-splenic changes in gene expression and methylation

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Journal of animal Science and Biotechnology 2022年第1期13卷 94-105页

作者： A.Dunislawska A.Slawinska M.Gryzinska M.Siwek Department of animal Biotechnology and Genetics UTP University of Science and Technology85-084 BydgoszczPoland Institute of Biological Basis of animal Production Sub-Department of General and Molecular GeneticsUniversity of Life Sciences in Lublin20-032 LublinPoland

Background:Epigenetic regulation of the gene expression results from interaction between the external environment and transcription of the genetic information encoded in *** CpG regions within the gene promoters lead to silencing of the gene expression in most *** contributing to epigenetic regulation include intestinal microbiota,which in chicken can be potently modified by in ovo *** main aim of this study was to determine global and specific methylation patterns of the spleen under the influence of host-microbiome ***:Fertilized eggs of two genotypes:Ross 308 and Green-legged Partridgelike were in ovo stimulated on d 12 of *** injected compounds were as follows:probiotic-Lactococcus lactis *** IBB477,prebiotic-galactooligosaccharides,and synbiotic-combination of *** were sacrificed on d 42 *** was collected,RNA and DNA were isolated and intended to gene expression,gene methylation and global methylation *** have proved that negative regulation of gene expression after administration of bioactive substances in ovo might have epigenetic *** changes depend on the genotype and the substance administered in ***:Epigenetic nature of microbial reprogramming in poultry and extension of issues related to hostmicrobiome interaction is a new direction of this research.

关键词： Gene expression Host-microbiome immune tissue interaction Methylation level

Differences of core genes in liver fibrosis and hepatocellular carcinoma:Evidence from integrated bioinformatics and immunohistochemical analysis

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World Journal of Gastrointestinal Oncology 2022年第7期14卷 1265-1280页

作者： Yue Li Shou-Li Yuan Jing-Ya Yin Kun Yang Xin-Gang Zhou Wen Xie Qi Wang Department of Pathology Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China Beijing Key laboratory of Emerging Infectious Diseases Beijing 100015China Key laboratory of animal Ecology and Conservation Biology Institute of ZoologyBeijing 100101China Center of Liver Diseases Beijing Ditan HospitalCapital Medical UniversityBeijing 100015China

BACKGROUND Liver fibrosis and hepatocellular carcinoma(HCC)are common adverse consequences of chronic liver *** interaction of various risk factors may cause them to *** of specific biomarkers is of great significance for understanding the occurrence,development mechanisms,and determining the novel tools for diagnosis and treatment of both liver fibrosis and *** To identify liver fibrosis-related core genes,we analyzed the differential expression pattern of core genes in liver fibrosis and *** Gene expression profiles of three datasets,GSE14323,GSE36411,and GSE89377,obtained from the Gene Expression Omnibus(GEO)database,were analyzed,and differentially expressed genes(DEGs)between patients with liver cirrhosis and healthy controls were identified by screening via R software packages and online tool for Venn *** WebGestalt online tool was used to identify DEGs enriched in biological processes,molecular functions,cellular components,and Kyoto Encyclopedia of Genes and Genomes *** protein–protein interactions of DEGs were visualized using Cytoscape with ***,the expression pattern of core genes was analyzed using Western blot and immunohistochemistry in a carbon tetrachloride(CCl_(4))-induced liver cirrhosis mouse model and in patient liver ***,Kaplan-Meier curves were constructed using the Kaplan-Meier plotter online *** Forty-five DEGs(43 upregulated and 2 downregulated genes)associated with liver cirrhosis were identified from three GEO *** hub genes were identified,which were upregulated in liver *** blot and immunohistochemical analyses of the three core genes,decorin(DCN),dermatopontin(DPT),and SRY-box transcription factor 9(SOX9),revealed that they were highly expressed in the CCl4-induced liver cirrhosis mouse *** expression levels of DCN and SOX 9 were positively correlated with the degree of fibrosis,and SOX 9 level in HCC patients was significant

关键词： Liver cirrhosis Hepatocellular carcinoma Bioinformatical analysis Decorin Dermatopontin SRY-box transcription factor 9

Effects of dietary replacement of soybean meal with dried distillers grains with solubles on the microbiota occupying different ecological niches in the rumen of growing Hu lambs

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Journal of animal Science and Biotechnology 2021年第2期12卷 677-688页

作者： Junshi Shen Zhipeng Li Zhongtang Yu Weiyun Zhu laboratory of Gastrointestinal Microbiology Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal HealthCollege of Animal Science and TechnologyNanjing Agricultural UniversityNanjing 210095China National Center for International Research on animal Gut Nutrition Nanjing Agricultural UniversityNanjing 210095China Department of Special animal Nutrition and Feed Science Institute of Special Animal and Plant SciencesChinese Academy of Agricultural SciencesChangchun 130112China Department of animal Sciences The Ohio State UniversityColumbusOH 43210USA

Background:Diet has a profound impact on the rumen microbiota,and the impact can vary among the different rumen ecological niches(REN).This study investigated the effects of dietary replacement of soybean meal(SBM)with dried distillers grains with solubles(DDGS)on the rumen microbiota occupying different REN of growing Hu *** a 9-week feeding trial,6 lambs from each dietary treatment(SBM ***-based diets)were slaughtered for sample *** microbiota of the rumen solid,liquid,and epithelium fractions was examined using amplicon sequencing analysis of bacterial 16S rRNA gene,functional prediction,and ***:No interaction of dietary protein source(PS)and REN were detected for virtually all the measurements made in this *** DDGS substitution resulted in very limited influence on bacterial community ***,the metabolic pathways predicted from 16S rRNA gene sequences varied greatly between SBM-and DDGS-based *** populations of rumen total bacteria,fungi,sulfate-reducing bacteria(SRB),and methanogens were not influenced by DDGS substitution,but the population of protozoa was *** bacterial communities in rumen solid(RS)and liquid(RL)were similar in taxonomic composition but were different in relative abundance of some *** contrast,the bacterial composition and relative abundance of rumen epithelium(RE)were greatly distinct from those of the RS and the *** alignment with the bacterial relative abundance,the metabolic pathways predicted from 16S rRNA genes also varied greatly among the different *** populations of total bacteria,protozoa,and methanogens attached to the RE were smaller than those in the RS and RL,and the fungal population on the rumen epitheliumwas smaller than that in the RS but similar to that in the *** the contrary,the SRB population on the RE was greater than that in the RS and ***:Substitution of SBM with DDGS had greater impact to the protozoa than to the other microbes,and

关键词： Distillers dried grains with solubles Growing lamb Microbiota Ruminal ecological niche Soybean meal

Prevotella-rich enterotype may benefit gut health in finishing pigs fed diet with a high amylose-to-amylopectin ratio

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animal Nutrition 2021年第2期7卷 400-411页

作者： Wen Ren Honglin Yan Bing Yu Maria C.Walsh Jie Yu Ping Zheng Zhiqing Huang Junqiu Luo Xiangbing Mao Jun He Hui Yan Daiwen Chen Yuheng Luo Key laboratory for animal Disease-Resistance Nutrition of Ministry of Education of China Key Laboratory for Animal Disease-Resistance Nutrition and Feed of Ministry of Agriculture of ChinaKey Laboratory of Animal Disease-resistant Nutrition of Sichuan ProvinceAnd Animal Nutrition InstituteSichuan Agricultural UniversityChengdu 611130China DSM(China)animal Nutrition Research Center Co. LtdBazhou 065799China School of Life Science and Engineering Southwest University of Science and TechnologyMianyangSichuan 621010China DSM Nutritional Products Kaiseraugst 4303Switzerland

To investigate the influence of baseline enterotypes and dietary starch type on the concentration of short-chain fatty acids(SCFA),numbers of butyrate producing bacteria and the expression of genes related to intestinal barrier and inflammatory response in the colon of finishing pigs,a 60-d in vivo trial was conducted.A 2-wk pre-trial with 102 crossbred(Duroc×[Landrace×Yorkshire])finishing barrows(90 d old)was conducted to screen ***,a total of 32 pigs(87.40±2.76 kg)with high(HPBR,>14)and low(LPBR,interaction(P<0.05)of HPBR and HAMR was also found to decrease the gene expression of interleukin(IL)-6,IL-12,IL-1βand tumor necrosis factor-α(TNF-α)in colonic *** findings show that HAMR diet increased the abundance and activity of butyrate-producing bacteria and the concentration and absorption of SCFA,which may be associated with the decreased gene expression of inflammatory cytokines in the colonic mucosa of pigs with Prevotella-rich *** these alterations are likely to have a pos

关键词： Enterotype Amylose-to-amylopectin ratio Gut health Finishing pig

A relatively high zoonotic trematode prevalence in Orientogalba ollula and the developmental characteristics of isolated trematodes by experimental infection in the animal model

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Infectious Diseases of Poverty 2022年第4期11卷 72-80页

作者： Jian Li Yijing Ren Lei Yang Jiani Guo Haiying Chen Jiani Liu Haoqiang Tian Qingan Zhou Weiyi Huang Wei Hu Xinyu Feng Basic Medical College Guangxi Traditional Chinese Medical UniversityNanning 530005GuangxiChina College of Life Sciences Inner Mongolia UniversityHohhot 010070China College of animal Science and Technology Guangxi UniversityNanning 530005GuangxiChina National Institute of Parasitic Diseases Chinese Center for Disease Control and PreventionKey Laboratory of Parasite and Vector Biology of China Ministry of HealthWHO Collaborating Centre for Tropical DiseasesJoint Research Laboratory of Genetics and Ecology On Parasite-Host InteractionChinese Center for Disease Control and PreventionFudan University200025 ShanghaiChina Department of Infectious Diseases Huashan HospitalState Key Laboratory of Genetic EngineeringMinistry of Education Key Laboratory for Biodiversity Science and Ecological EngineeringMinistry of Education Key Laboratory of Contemporary AnthropologySchool of Life SciencesFudan UniversityShanghai 200438China School of Global Health Chinese Center for Tropical Diseases ResearchShanghai Jiao Tong University School of MedicineShanghai 20025China One Health Center Shanghai Jiao Tong University-The University of EdinburghShanghai 20025China

Background:Food-borne parasitic diseases decrease food safety and threaten public *** snail species is an intermediate host for numerous human parasitic *** ollula has been reported as intermediate hosts of many zoonotic ***,we investigated the prevalence of zoonotic trematodes *** in Guangxi,China,and assessed their zoonotic ***:Snails were collected from 54 sites in 9 cities throughout *** snail and trematode larvae species were determined by combining morphological characteristics and molecular *** trematodes prevalence and constituent ratio were calculated and compared among different habitat *** trees of the trematode species were constructed using the neighbor-joining method with nuclear internal transcribed spacer 2(ITS2)*** developmental cycles of the isolated trematodes were examined by experimental infection in *** developmental characteristics ofEchinostoma revolutum was recorded by dissecting infected ducklings from 1-day post infection(dpi)to 10 ***:The overall prevalence of trematode larvae was 22.1%(1818/8238)*** from 11 sample *** together with molecular identification,showed ***,Australapatemon sp.,Hypoderaeum conoideum,Pharyngostomum cordatum,andEchinostoma ***,with the highest infection rate ***(13.0%).However,noFasciola larvae were *** trematodes prevalence and constituent ratio varied in two sub-biotypes(Panimal model forEchinostoma revolutum was successfully *** matured from larvae to adult at 10 dpi in the intestine of the duck,and the devel

关键词： Orientogalba ollula Zoonotic trematode Intermediate host Prevalence ITS2

Swine gut microbiota and its interaction with host nutrient metabolism

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animal Nutrition 2020年第4期6卷 410-420页

作者： Hongyu Wang Rongying Xu He Zhang Yong Su Weiyun Zhu laboratory of Gastrointestinal Microbiology Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal HealthCollege of Animal Science and TechnologyNanjing Agricultural UniversityNanjing210095China National Center for International Research on animal Gut Nutrition Nanjing Agricultural UniversityNanjing210095China

Gut microbiota is generally recognized to play a crucial role in maintaining host health and *** correlation among gut microbiota,glycolipid metabolism,and metabolic diseases has been well reviewed in ***,the interplay between gut microbiota and host metabolism in swine remains incompletely *** the limitation in conducting human experiments and the high similarity between swine and humans in terms of anatomy,physiology,polyphagy,habits,and metabolism and in terms of the composition of gut microbiota,there is a pressing need to summarize the knowledge gained regarding swine gut microbiota,its interplay with host metabolism,and the underlying *** review aimed to outline the bidirectional regulation between gut microbiota and nutrient metabolism in swine and to emphasize the action mechanisms underlying the complex microbiomeehost crosstalk via the gut microbiotaegutebrain ***,it highlights the new advances in knowledge of the diurnal rhythmicity of gut microbiota.A better understanding of these aspects can not only shed light on healthy and efficient pork production but also promote our knowledge on the associations between gut microbiota and the microbiomeehost crosstalk *** importantly,knowledge on microbiota,host health and metabolism facilitates the development of a precise intervention therapy targeting the gut microbiota.

关键词： Gut microbiota Host nutrient metabolism Microbiotaegutebrain axis Microbiota diurnal rhythmicity

Vanadium in high-fat diets sourced from egg yolk decreases growth and antioxidative status of Wistar rats

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animal Nutrition 2019年第3期5卷 307-313页

作者： Jian-Ping Wang Ren-Yong Cui Xue-Mei Ding Shi-Ping Bai Qiu-Feng Zeng Huan-Wei Peng Ke-Ying Zhang animal Nutrition Institute Key Laboratory of Animal Disease-Resistance Nutrition Ministry of Education Ministry of Agriculture and Sichuan Province Sichuan Agricultural University

The objective of this paper was to evaluate the effect of vanadium(V)in high-fat diets sourced from egg yolk on body weight gain,feed intake,blood characteristics and antioxidative status of Wistar rats.A total of 72 female Wistar rats were allocated according to a 2 x 4 factorial design throughout a 5-wk trial,including 2 levels of dietary fat(normal and high;ether extract 40.3 and 301.2 g/kg;fat sourced from egg yolk)and 4 levels of dietary V(0,3,15 and 30 mg/kg).Vanadium decreased(Pinteraction,P<0.05).Under the same dietary V dose,V residual content in liver(dietary V at 15 and30 mg/kg)and kidney(dietary V at 15 mg/kg)was higher in high-fat diet treatment compared with normal-fat diet treatment(P<0.05).In conclusion,it is suggested that V could decrease the body weight together with the feed intake,and the high fat could enhance oxidative stress induced by V of Wistar rats.

关键词： Vanadium High fat Oxidative stress Body weight Wistar rats

cDNA Cloning,Sequence Analysis of the Porcine LIM and Cysteine-rich Domain 1 Gene

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Acta Biochimica et Biophysica Sinica 2005年第12期37卷 843-850页

作者： Jun WANG Chang-Yah DENG Yuan-Zhu XIONG Bo ZUO Lei XING Feng-E LI Ming-Gang LEI Rong ZHENG Si-Wen JIANGKey Laboratiry of Swine Genetics and Breeding,Ministry of Agriculture.College of animal Sciences,Huazhong Agricultural University.Wuhan 430070,China Key laboratory of Swine Genetics and Breeding Ministry of Agriculture College of Animal Sciences Huazhong Agricultural UniversityWuhan 430070 China

LIM domain proteins are important regulators in cell growth,cell fate determination,celldifferentiation and remodeling of the cell cytoskeleton by their interaction with various structural proteins,kinases and transcriptional *** molecular biology combined with in silico cloning,we havecloned the complete coding sequence of pig LIM and the cysteine-rich domain 1 gene (LMCD1) whichencodes a 363 amino acid *** estimated molecular weight of the LMCD1 protein is 40,788 Da witha pI of *** was found to be highly expressed in both skeletal muscle and cardiac *** revealed that the deduced protein sequence shares 86%,91% and 93% homology with that of itshuman,mouse and rat counterparts,*** LMCD 1 protein was predicted by bioinformaticssoftware to contain a novel cysteine-rich domain in the N-terminal region,two LIM domains in theC-terminal region,nine potential protein kinase C phosphorylation sites,seven casein kinase Ⅱ phosphorylationsites,a tyrosine kinase phosphorylation site,seven N-glycosylation and N-myristoylation sites and a singlepotential N-glycosylation site,which is similar to the protein’s human *** tree wasconstructed by aligning the amino acid sequences of the LIM domain trom different *** addition,fourbase mutations were detected by comparing the sequences of Large White pigs with those of ChineseMeishan *** G294A mutation site was confirmed by polymerase chain reaction-single-strandconformation polymorphism *** allele frequencies were studied in five pig breeds.

关键词： LIM and cysteine-rich domain 1(LMCD1) SSCP PCR gene expression data analysis

Evodiamine derivatives improve cognitive abilities in APP^(swe)/PS1^(ΔE9) transgenic mouse models of Alzheimer's disease

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animal Models and Experimental Medicine 2020年第2期3卷 193-199页

作者： Shuo Pang Caixian Sun Shan Gao Yajun Yang Xiandao Pan Lianfeng Zhang Key laboratory of human Disease Comparative Medicine National Health Commission of China(NHC)Institute of Laboratory Animal SciencePeking Union Medical CollegeChinese Academy of Medical SciencesBeijingChina Beijing Engineering Research Center for Experimental animal Models of human Diseases Institute of Laboratory Animal SciencePeking Union Medical CollegeChinese Academy of Medical SciencesBeijingChina Beijing Key laboratory of Active Substance Discovery and Drug ability Evaluation Institute of Material Medical Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Neuroscience Center Chinese Academy of Medical SciencesBeijingChina

Background: Alzheimer's disease(AD) is a complex neurodegenerative disease. Due to the complexity of its molecular pathogenesis and the interaction of the numerous factors involved, the etiology and pathogenesis of AD have not been fully elucidated. Therefore, effective treatment for AD remains to be developed. Evodiamine, a quinolone alkaloid, has been found to improve learning and memory ability to in the APP^(swe)/PS1^(ΔE9) mouse model of dementia. However, the cytotoxicity and physicochemical properties of evodiamine have limited its use in the treatment of ***: Evodiamine and its derivatives were effectively synthesized by EDCImediated condensation at room temperature. These target compounds contained 1 thio-and 21 oxo-evodiamine derivatives with different substituted groups. The cytotoxicity of evodiamine and its derivatives and the neuroprotective effects of the evodiamine derivatives against H_2O_2-induced cell loss in SH-SY5 Y cells were investigated using the WST-8 assay. The Morris water-maze test was used to detect the effect of evodiamine and its derivatives on improving learning and memory in APP^(swe)/PS1^(ΔE9) ***: In this study, a series of oxo-and thio-evodiamine derivatives was synthesized. Several derivatives showed lower cytotoxicity and stronger neuroprotective effects than evodiamine and elicited enhanced cognitive improvement, especially in the test of spatial memory in APP^(swe)/PS1^(ΔE9) ***: Our study provides insights for developing novel evodiamine derivatives for chemical intervention and treatment of AD.

关键词： evodiamine derivatives mouse model neuroprotective spatial memory