Prevotella-rich enterotype may benefit gut health in finishing pigs fed diet with a high amylose-to-amylopectin ratio

作     者：Wen Ren Honglin Yan Bing Yu Maria C.Walsh Jie Yu Ping Zheng Zhiqing Huang Junqiu Luo Xiangbing Mao Jun He Hui Yan Daiwen Chen Yuheng Luo Wen Ren;Honglin Yan;Bing Yu;Maria C.Walsh;Jie Yu;Ping Zheng;Zhiqing Huang;Junqiu Luo;Xiangbing Mao;Jun He;Hui Yan;Daiwen Chen;Yuheng Luo

作者机构：Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education of ChinaKey Laboratory for Animal Disease-Resistance Nutrition and Feed of Ministry of Agriculture of ChinaKey Laboratory of Animal Disease-resistant Nutrition of Sichuan ProvinceAnd Animal Nutrition InstituteSichuan Agricultural UniversityChengdu 611130China DSM(China)Animal Nutrition Research Center Co.LtdBazhou 065799China School of Life Science and EngineeringSouthwest University of Science and TechnologyMianyangSichuan 621010China DSM Nutritional ProductsKaiseraugst 4303Switzerland 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2021年第7卷第2期

页      面：400-411页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：The National Natural Science Foundation of China(NSFC grant number 31730091 31872369 and 31672436) 

主　　题：Enterotype Amylose-to-amylopectin ratio Gut health Finishing pig 

摘      要：To investigate the influence of baseline enterotypes and dietary starch type on the concentration of short-chain fatty acids(SCFA),numbers of butyrate producing bacteria and the expression of genes related to intestinal barrier and inflammatory response in the colon of finishing pigs,a 60-d in vivo trial was conducted.A 2-wk pre-trial with 102 crossbred(Duroc×[Landrace×Yorkshire])finishing barrows(90 d old)was conducted to screen ***,a total of 32 pigs(87.40±2.76 kg)with high(HPBR,14)and low(LPBR,2)Prevotella-to-Bacteroides ratios(PBR)in equal measure were selected and randomly divided into 4 groups with 8 replicates per group and 1 pig per *** trial was designed following a 2(PBR)×2(amylose-to-amylopectin ratio,AMR)factorial *** with different PBR were fed diets based on corn-soybean meal with high AMR(HAMR,1.24)or low AMR(LAMR,0.23),*** showed that neither PBR nor AMR influenced the growth performance of *** pigs fed HAMR diet had a higher number of colonic Clostridium cluster XIVa and higher gene expression of butyrate kinase compared to the LPBR pigs(P0.05).The HPBR pigs fed HAMR diets also had increased colonic concentrations of total SCFA and propionate compared to the LPBR pigs(P0.05).Comparing with other pigs,HPBR pigs fed HAMR diets showed a lower(P0.05)expression of histone deacetylases(HDAC)gene and higher(P0.05)expression of G protein-coupled receptor 43 gene(GPR 43)in the colonic *** interaction(P0.05)of HPBR and HAMR was also found to decrease the gene expression of interleukin(IL)-6,IL-12,IL-1βand tumor necrosis factor-α(TNF-α)in colonic *** findings show that HAMR diet increased the abundance and activity of butyrate-producing bacteria and the concentration and absorption of SCFA,which may be associated with the decreased gene expression of inflammatory cytokines in the colonic mucosa of pigs with Prevotella-rich *** these alterations are likely to have a pos