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Diet and feeding ecology of invasive icefish Neosalanx taihuensis in Erhai Lake,a Chinese plateau mesoeutrophicated lake

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Chinese Journal of Oceanology and Limnology 2015年第2期33卷 372-380页

作者：胡翠林过龙根王圣瑞 fisheries College Huazhong Agricultural University Donghu Experimental Station of Lake Ecosystems State Key Laboratory of Freshwater Ecology and Biotechnology of ChinaInstitute of HydrobiologyChinese Academy of Sciences Research Center of Lake Environment Chinese Research Academy of Environment Sciences

A comprehensive study of the invasive icefish Neosalanx taihuensis feeding ecology in Erhai Lake was conducted from November 2009 to October 2010. Prey items in the guts of the icefish sampled varied significantly according to season. This finding suggests a relationship between fluctuations in available prey in the environment and selective feeding by icefish. N. taihuensis preferred large-sized zooplankton, such as Daphnia and calanoids. Additionally, the gut fullness values differed significantly （P〈0.001） among sampling times. To compare the values at different times, samples were taken over a 24 h period every 2 months for the entire year. The feeding activities of the fish were concentrated either in the morning （8：00） and/or at dusk （20：00）, except in September 2010. This finding can be explained primarily by the variation in optimum light intensity. Daily N. taihuensis zooplankton consumption varied significantly, both diurnally and among seasons. The daily consumption values ranged from 0.089 to 0.237 g （wet weight） per 100 g wet fish weight in temperatures between 11.50℃ and 24.68℃. This is the first report of diel feeding periodicity and daily food consumption of icefish in their invaded ecosystems.

关键词： dietary composition crustacean zooplankton feeding ecology invasive species Neosalanxtaihuensis

Karyotype Analysis of Cyprinus carpio in Dongping Lake

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Animal Husbandry and Feed Science 2017年第6期9卷 394-397页

作者： Lu Hong Meng Qinglei Yang Ling Shandong Provincial Key laboratory of freshwater Fishery Genetics and Breeding Shandong Freshwater Fisheries Research Institute

To investigate the genetic composition of the carp （Cyprinus carpio） in Dongping Lake and to provide eytogenetie data for exploration, protection and appropriate utilization of the germplasm resources, karyotype of the carp was analyzed in this study. In detail, colehieine was given to the fish an intraperitoneal route 24 h after the intraperitoneal injection of phytohemagglutinin （PHA） solution to arrest cells in metaphase. The kidney cells were collected for the preparation of chromosome specimens, which were observed under a microscope. Ten cells having correct number of chromosomes and clear morphology were photographed and zoomed to measure the relative length, arm ratio, ann number （NF） and other parameters of chromosomes. The results showed that the chromosome number of C. earpio in Dongping Lake was （2n = 100） ; the karyotype formula was （2n =20m ＋38sm ＋20st ＋22t）. The total number of chromosome arms （NF） was 158. By comparing the karyotype of Dongping Lake carp with that of other Cyprinus species, we concluded that Dongping Lake carp is more highly evolved among Cyprinus species.

关键词： Cyprinus carpio Dongping Lake Chromosome Karyotype

Study on abundance variation of pteropods in the East China Sea

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Acta Oceanologica Sinica 2006年第1期25卷 100-107页

作者： XU Zhaoli LI Chunju Key and Open laboratory of Marine and Estuary fisheries of Ministry of Agriculture of China East China Sea Fisheries Research Institute Chinese Academy of Fisheries Sciences Shanghai 200090 China

On the basis of the census in four seasons from 1997 to 2000 in the East China Sea （23°30＇-33°N, 118°0＇-128°E）, the horizontal distribution of pteropods, the seasonal variations of their abundance and dynamic mechanisms of these variations were approached with quantitative and qualitative methods. Results showed that the total abundance of pteropods varied clearly with the seasons in an order of decrease of autumn （108×10-2 ind./m^3）, summer （33×10^-2 ind./m^3）, winter（28×10^-2 ind./m^3） and spring （10×10^-2 ind./m^3）. Pteropods were not observed in the north nearshore of the East China Sea （29°30＇-33°N, 122°30＇-125°E）in winter and spring. In all seasons, the peteropod abundance was higher in the south than that in the north and higher in the offshore than that in the nearshore. The major species influencing the peteropod abundance were Creseis acicula in spring, Creseis virgula in summer, Desmopterus papilio in autumn and Limaeina trochiforrnis in winter. The variation of pteropod abundance showed a prominent linear correlation with the surface water temperature and 10 m water temperature, but no significant correlation with both the bottom water temperature and salinity. In summer, the high-abundance distribution area of pteropods was located in the place where the Taiwan Warm Current branched from the Kuroshio. Inautumn, thedistribution area with anabundance higher than 250×10^-2ind/m^3expanded northward along with the Taiwan Warm Current within the mixing area where the Changjiang freshwater, the Fujian-Zhejiang coastal water and the Huanghai Sea Cold Water met together. Water temperature and warm currents were the most important environmental factors that influenced the changes ofpteropod abundance in the East China Sea.

关键词： East China Sea ptcropods zooplankton abundance distribution regression contribution

Length-weight relationships for six freshwater fish species in Iran

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Chinese Journal of Oceanology and Limnology 2009年第1期27卷 61-62页

作者： M. S. HEYDARNEJAD Fish and fisheries laboratory Zoology Dept. Shahrekord University

The aim of this study is to report the length-weight relationship parameters （a and b） for some economically important fish species from Iranian freshwaters. The length-weight relationships （LWR） are calculated for six freshwater fish species collected during 2006 and 2007 in Iran. The values of the exponent b in the LWR （W=aL^b） vary between 2.985 and 3.543. The LWR with high correlation coefficient （r^2） is significant for all the species. These parameters axe of great importance to evaluate the relative condition of populations, biology, species management and their fisheries and stock assessment. The application of the length-weight relationships presented here should be limited to the observed length ranges.

关键词： length-weight relationships (LWR) freshwater fishes in Iran isometric and allometric growths

Quantitative trait loci for morphometric traits in multiple families of common carp(Cyprinus carpio)

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Science China(Life Sciences) 2017年第3期60卷 287-297页

作者： Xianhu Zheng Youyi Kuang Weihua Lv Dingchen Cao Zhipeng Sun Wu Jin Xiaowen Sun Heilongjiang River fisheries Research Institute Chinese Academy of Fishery SciencesHarbin 150070China College of Life Science Northeast Agricultural UniversityHarbin 150030China freshwater fisheries Research Center Chinese Academy of Fishery SciencesWuxi 214081China

Quantitative trait locus(QTL) mapping is frequently used to understand the genetic architecture of quantitative ***,we performed a genome scanfor QTL affecting the morphometric characters in eight full-sib families containing 522 individuals using different statistical methods(Sib-pair and half-sib model).A total of 194 QTLs were detected in 25 different regions on 10 linkage groups(LGs).Among them,37 QTLs on five LGs(eight,13,24,40 and 45) were significant(5%genome-wide level),while the remaining 40(1%chromosome-wide level) and 117(5%chromosome-wide level) indicated suggestive effect on those *** for most morphometric traits were moderate to high,ranging from 0.21 to 0.66,with generally strong phenotypic and genetic correlations between the traits.A large number of QTLs for morphometric traits were co-located,consistent with their high correlations,and may reflect pleiotropic effect on the same *** pathways were mapped for possible candidate genes on QTL *** significantly enriched pathway was identified onLG45,which had a P-value of 0.04 and corresponded to the "regulation of actin cytoskeleton pathway".The results are expected to be useful in marker-assisted selection(MAS) and provide valuable information for the study of gene pathway for morphometric and growth traits of the common carp.

关键词： common carp morphometric traits QTL heritability pathway

Effect of temperature on pearl deposition rate and matrix protein gene expressions in Hyriopsis cumingii

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Aquaculture and fisheries 2023年第1期8卷 76-84页

作者： Can Jin Xin Zhang Xiaojun Liu Jiale Li Shaoxing University Shaoxing312000China Key laboratory of freshwater Aquatic Genetic resources Ministry of Agriculture and Rural AffairsShanghai201306China National Demonstration Center for Experimental fisheries Science Education Shanghai Ocean UniversityShanghai201306China Shanghai Engineering Research Center of Aquaculture Shanghai201306China

In this study,we investigated the impact of water temperature on pearl formation and the molecular mechanisms underlying this biomineralization process in the mussel Hyriopsis *** the laboratory experiment,recipient mussels received saibo grafts from donor mussels and then were cultivated at five temperatures(16,20,24,28,and 32◦C)for 1 *** found that speed of pearl formation varied with water temperature:there was no visible pearl granules at 16 and 20◦C,tawny pearl granules were collected at 24◦C and lustrous pearl granules were collected at 28 and 32◦*** expressions of several nacreous layer matrix protein genes(hic22,hic24,HcTyr Hc-upsalin and Hcperlucin)at 28◦C and 32◦C were peaked on day 28,and the expressions of other nacreous layer matrix protein genes(hic52 and hic74)on day 28 were significant higher than day *** the natural environment,pearl deposition rate also increased as water temperature rose,and the optimum range for pearl growth in *** was between 28.3 and 30.3◦*** of the prismatic layer-related matrix protein genes hic31 and both prismatic and nacreous layer-related matrix protein gene silkmapin was significantly high in winter,and expressions of the nacreous layer-related matrix protein genes(hic24,hic74,HcTyr,Hc-upsalin and Hcperlucin)were always higher during higher temperature seasons compared with *** results indicate that high water temperature can speed up pearl formation and further regulate matrix protein secretion to accelerate pearl nacre deposition.

关键词： Pearl Temperature Matrix protein Hyriopsis cumingii

Chronic exposure to high-density polyethylene microplastic through feeding alters the nutrient metabolism of juvenile yellow perch (Perca flavescens)

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Animal Nutrition 2022年第2期9卷 143-158页

作者： Xing Lu Dong-Fang Deng Fei Huang Fabio Casu Emma Kraco Ryan J.Newton Merry Zohn Swee J.Teh Aaron M.Watson Brian Shepherd Ying Ma Mahmound A.O.Dawood Lorena M.Rios Mendoza School of freshwater Sciences University of WisconsinMilwaukeeWI 53204USA South Carolina Department of Natural resources CharlestonSC 29412USA USDA/ARS/School of freshwater Sciences University of WisconsinMilwaukeeWI 53204USA School of Veterinary Medicine Department of AnatomyPhysiologyand Cell BiologyUniversity of CaliforniaDavisCA 95616USA Department of Natural Sciences Marine Resources Research InstituteUniversity of WisconsinSuperiorWI 54880USA Yangtze River fisheries Research Institute Chinese Academy of Fishery SciencesWuhanChina fisheries College Jimei UniversityXiamenChina. Faculty of Agriculture Kafrelsheikh UniversityKafr El-SheikhEgypt.

Microplastics are emergent contaminants threatening aquatic organisms including aquacultured *** study investigated the effects of high-density polyethylene(HDPE,100 to 125 mm)on yellow perch(Perca flavescens)based on integrative evaluation including growth performance,nutritional status,nutrient metabolism,fish health,and gut microbial *** test diets(0,1,2,4,or 8 g HDPE/100 g diet)containing 41%protein and 10.5%lipid were fed to juvenile perch(average body weight,25.9±0.2 g;n=15)at a feeding rate of 1.5%to 2.0%body weight *** feeding trial was conducted in a flow-through water system for 9 wk with 3 tanks per treatment and 15 yellow perch per *** mortality or HDPE accumulation in the fish was found in any *** gain and condition factor of fish were not significantly impacted by HDPE(P>0.05).Compared to the control group,fish fed the 8%HDPE diet had significantly decreased levels of protein and ash(P<0.05).In response to the increasing levels of HDPE exposure,the hepatosomatic index value,hepatocyte size,and liver glycogen level were increased,but lipid content was reduced in the liver *** to the control treatment,fish fed the 8%HDPE diet had significant accumulations of total bile acids and different metabolism pathways such as bile acid biosynthesis,pyruvate metabolism,and carnitine *** enterocyte necrosis was documented in the foregut of fish fed the 2%or 8%HDPE diet;and significant cell sloughing was observed in the midgut and hindgut of fish fed the 8%HDPE *** fed the 2%HDPE diet harbored different microbiota communities compared to the control *** study demonstrates that HDPE ranging from 100 to 125 mm in feed can be evacuated by yellow perch with no impact on ***,dietary exposure to HDPE decreased whole fish nutrition quality,altered nutrient metabolism and the intestinal histopathology as well as microbiota community of yellow *** results indicate that extende

关键词： Dietary exposure Intestinal microbiota Liver metabolomics Microplastics Nutrient composition Yellow perch

Prokaryotic expression of goldfish Tgf2 transposase with optimal codons and its enzyme activity

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Aquaculture and fisheries 2019年第3期4卷 98-104页

作者： Xi Zhao Ruirui Si Mingjuan He Xiayun Jiang Shuming Zou College of Food Science and Technology Shanghai Ocean UniversityShanghai201306China Genetics and Breeding Center for Blunt Snout Bream Key Laboratory of Freshwater Aquatic Genetic ResourcesNational Demonstration Center for Experimental Fisheries Science EducationShanghai Ocean UniversityShanghai201306China

Tgf2 transposase(Tgf2-TPase),a hAT transposase from goldfish,plays an important role in fish transgenic ***,the production of the recombinant Tgf2-TPase protein required rigorous fermentation at low temperatures(22℃)and early log phase induction(OD600=0.3–0.4)in Rosetta 1(DE3)Escherichia coli *** order to better express the Tgf2-TPase and detect its enzyme activity,83 rare codons in Tgf2-TPase were optimized and designated Tgf2-TPase^(83).The expression results showed that the soluble recombinant Tgf2-TPase83 was highly expressed at 30℃ and was inducible at an OD600 of 0.5–0.6 in the same prokaryotic expression *** purification by affinity chromatography,Tgf2-TPase83 with codon optimization had higher enzyme activity than the Tgf2-TPase *** of different preservation methods(freezedrying at−80℃,storage in 20%-glycerol,8%-sucrose,4%-mannitol),revealed storage of Tgf2-TPase^(83) in glycerol helped to preserve its DNase digestion ***,size exclusion chromatography suggested that the purified Tgf2-TPase^(83) could recognize and bind to DNA probes containing a terminal inverted repeat(TIR)and a subterminal repeat(STR)sequence of the Tgf2 ***,the results showed that optimizing the 83 codons of Tgf2 transposase can simplify the fermentation process and improve the enzyme *** propose that the production of the Tgf2-Tpase83 protein in a soluble and active form could provide an alternative tool for genetic modification of fish.

关键词： Codon optimization Enzyme activity Prokaryotic expression Tgf2 transposase

Effects of 1-Naphthol on Physiological Parameters of Chlorella vulgaris

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Agricultural Biotechnology 2014年第4期3卷 45-48,52页

作者： Liping QIU Yonghong LUO Jianhong QU Gengdong HU Jiazhang CHEN Key Open laboratory of Ecological Environment and resources of Inland fisheries Freshwater Fisheries Research CenterChinese Academy of Fishery Sciences Wuxi Fishery College Nanjing Agricultural University

[ Objective] This study aimed to investigate the effects of 1-naphthol on primary productivity in aquatic ecosystems. [ Method] The effects of 1-naphthol on biomass, chlorophyll a content, malondialdehyde （MDA） content and soluble protein content of Chlorella vulgaris were investigated. [ Result] 1-Naphthol could significantly inhibit the growth of Chlorella vulgaris. On the 7^th d, the biomass of Chlorella vulgaris exposed to 1.0, 3.0, 6.0, 12.0 and 18.0 mg/L 1-naphthol was 60.26%, 48.15%, 41.32%, 28.59% and 27.10% of that in control respectively, exhibiting a significant dose-response relationship. Chlorella vulgaris was exposed to different concentrations of 1-naphthol （0, 1,5 and 10 mg/L） for 7 d ; with the increase of 1-naphthol concentration, chlorophyll a content of Ch/orella vulgaris was reduced, MDA content increased gradually, soluble protein content increased first and then declined. [ Conclusion] According to the experimental results, 1-naphthol can significantly inhibit the growth of Chlorella vulgaris, thus reducing primary productivity in aquatic ecosystems.

关键词： 1-Naphthol Chlorella vulgaris Biomass Physiological parameter