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Tecia solanivora infestation increases tuber starch accumulation in Pastusa Suprema potatoes

Journal of Integrative Plant Biology 2018年第11期60卷 1083-1096页

作者： Pavan Kumar Etzel Garrido Kun Zhao Yi Zheng Saleh Alseekh Erandi Vargas-Ortiz Alisdair R.Fernie Zhangjun Fei Katja Poveda Georg Jander Boyce Thompson Institute for Plant Research 533 Tower RoadIthacaNY 14853USA Department of entomology Cornell UniversityIthacaNY 14853USA Max Planck Institute of molecular Plant Physiology Wissenschaftspark GolmAm Muhlenberg 114476 PotsdamGermany department of entomology and nematology university of floridaGainesvilleFL 32611USA unidad de microbiologia basica y aplicada facultad de ciencias naturalesuniversidad autonoma de queretaroAv.de las Ciencias SINJuriquillaquerdtaroMdxico department of molecular biology institute for scientific and technological research of san luis potosiMexico

In response to infestation with larvae of the Guatemalan tuber moth （Tecia solanivora）, some Solanum tuberosum （potato） varieties exhibit an overcompensa- tion response, whereby the total dry mass of uninfested tubers is increased. Here, we describe early responses, within the first few days, of T. solanivora feeding, in the Colombian potato variety Pastusa Suprema. Non- targeted metabolite profiling showed significant second- ary metabolism changes in T. solanivora-infested tubers, but not in uninfested systemic tubers. In contrast, changes in primary metabolism were greater in unin- fested systemic tubers than in the infested tubers, with a notable 80% decline in systemic tuber sucrose levels within 1 d of T. solanivora infestation. This suggested either decreased sucrose transport from the leaves or increased sink strength, i.e., more rapid sucrose to starch conversion in the tubers. Increased sucrose synthesis was indicated by higher rubisco activase and lower starch synthase gene expression in the leaves of infested plants. Elevated sink strength was demonstrated by 45% more total starch deposition in systemic tubers of T. solanivord- infested plants compared to uninfested control plants. Thus, rather than investing in increased defense of uninfested tubers, Pastusa Suprema promotes deposition of photoassimilates in the form of starch as a response to T. solanivora infestation.

关键词： Tecia solanivora infestation increases tuber starch accumulation in Pastusa Suprema potatoes Figure

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Leap forward with insect genomics

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Insect Science 2016年第3期23卷 332-334页

作者： Bin Chen Xian-Hui Wang Zhijian Tu Yong-Ping Huang Institute of entomology and molecular Biology Chongqing Normal University Chongqing China State Key laboratory of IntegratedManagement of Pest Insects & Rodents Institute of Zoology Chinese Academy of Sciences Beijing China Department of Biochemistry Fralin Life Science Institute and Program in Genetics Bioinformatics and Computational Biology Virginia Tech Blacksburg Virginia USAand Key laboratory of Insect Development and Evolutionary Biology Institute of Plant Physiology and Ecology Shanghai Institutes forBiological Sciences Chinese Academy of Sciences Shanghal China

Insects comprise the largest animal group in the world. More than a million insect species have been identified, leaving possibly two to three times more to be discovered. Systematic understanding of the genetic content of an insect species began more than 15 years ago when the genome sequence of the fruit fly, Drosophila melanogaster was reported （Adams et al., 2000）. For the first time, scientists were able to glean unprecedented insights into the genetic blueprint of a model insect, presenting its 13 600 protein coding genes. Such fundamental information opened a floodgate for comparative and functional analysis of these genes and enabled systems approaches to comparative, evolutionary and population genomic studies. In recent years, next-generation and third-generation sequencing platforms brought genome sequencing to more than a hundred insect species, reach- ing far beyond the few well-studied model organisms. The international consortium for insect genomes is work- ing toward an ambitious goal to sequence 5000 species of insects.

关键词： Drosophila melanogaster Insect genetics DNA sequencing Genetic code Insect evolution Insect population genetics

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Cloning, Characterization and Bioinformatic Analysis of the Gene Encoding the Larval Serum Protein 2 in Diapause of the Onion Maggot, Delia Antiqua

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Engineering（科研） 2013年第10期5卷 487-490页

作者： Jingjing Xu Bin Chen Zhengbo He Youjin Hao Institute of entomology and molecular Biology College of Life Sciences Chongqing Normal University Shapingba Chongqing China nstitute of entomology and molecular Biology College of Life Sciences Chongqing Normal University Shapingba Chongqing China

The full-length cDNA encoding Larval serum protein 2 (LSp-2) in the onion maggot,Delia antiqua, was cloned and sequenced by rapid ampli?cation of cDNA ends methods. The result showed that the cDNA was 2203 bp long and the open reading frame (ORF) of 2106 bp encoded 701 amino acid with a calculated molecular weight of 80.5 kDa and an isoelectric point of 5.87. The onion maggot LSp-2 shows highest homology (83%) to that ofCalliphora vicinaat amino acid level. Its signal peptides, domains and structures were predicted and analyzed by using bioinformatic methods. The amino acid sequence of LSP-2 suggests that it would be a typical hexamerin.

关键词： Delia Antiqua Diapause Larval Serum Protein 2 Cloning molecular Characterization Bioinformatic Analysis

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Wildfire severity alters soil microbial exoenzyme production and fungal abundances in the southern Appalachian Mountains

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Pedosphere 2024年第5期34卷 865-878页

作者： Megan L.SCHILL Richard BAIRD Shawn P.BROWN Allison M.VEACH Department of Integrative Biology University of Texas at San AntonioSan Antonio TX 78249USA Biochemistry Molecular BiologyEntomology and Plant Pathology DepartmentMississippi State UniversityStarkville MS 39762USA Department of Biological Sciences The University of MemphisMemphis TN 38152USAAntonioand the University of Memphis。

Climate change has increased drought frequency and duration that are exacerbated by increased temperatures *** effect has,and will continue,to increase fire occurrence across many regions of North *** the southern Appalachian Mountains,wildfires with high burn severity occurred in 2016due to increased drought and human *** investigate the effects of burn severity on soil physicochemical properties,microbial extracellular enzyme production,and microbial abundances in a temperate region,surface soils(0-15 cm)were collected from two sites(the Great Smoky Mountains National Park in Tennessee and the Nantahala National Forest in North Carolina,USA)spanning lightly,moderately,and severely burned areas,accompanied by adjacent unburned locations that act as *** soil samples were collected at three time points between 2017 and 2019(i.e.,0.5,1,and 2.5 years post-fire)among burn severity *** hydrolytic enzyme production varied over time,with severe burn plots having significantly lower enzyme production at 2.5years *** enzymes varied among burn severities and across time *** burn plots showed greater carbon-specific(β-glucosidase andβ-xylosidase)and phosphorus-specific(acid phosphatase)enzyme activities at 0.5 years post-fire,but this effect was *** 2.5 years post-fire,theβ-xylosidase and acid phosphatase activities were lower in severe or moderate burn plots relative to the *** contrast,the activity of nitrogen-specific enzyme leucyl aminopeptidase was the lowest in severe burn plots at 0.5 years post-fire,but was the lowest in light burn plots at 2.5 years *** fungi:bacteria ratio declined with burn severity,indicating that fungi are sensitive or less resilient to high burn severity during *** results suggest that wildfires alter trajectories for soil microbial structure and function within a 2.5-year timeframe,which potentially has long-term impacts on biogeochemical

关键词： burn severity extracellular enzyme fungi:bacteria ratio internal transcribed spacer microbial structure 16S rRNA

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molecular detection of Leishmania isolated from Cutaneous leishmaniasis patients in Jask County,Hormozgan Province,Southern Iran,2008

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Asian Pacific Journal of Tropical Medicine 2012年第7期5卷 514-517页

作者： Koroush Azizi Aboozar Soltani Hamzeh Alipour Department of Medical entomology Schools of Health and NutritionShiraz University of Medical SciencesShirazIran Deparment of Medical entomology and Vector Control School of Public HealthTehran University of Medical SciencesTehranIran

Objective:To investigate on patients leishmanial infections in Jask ***: Impression smears were prepared from patients in 2008,all,were chequed for leishmanial infection by microscopy and molecular *** *** was extracted using Proteinase K and Phenol/Chloroform/Isoamyl alcohol *** variable segment on minicircles of kineloplasl DIVA was amplified via a Nested-PCR technique using species-specific primers(LIN R4-LIN 17 -Lin 19).Results:A total of 40 smears were prepared from 20 patients,from which,eight samples (40%) were positive for leishman body by microscopic method,while,18 samples(90%) were positive,*** parasite was identified as Leishmania major(L major).Conclusions: Zoonotic or Rural cutaneous leishmaniasis is endemic in Jask County whose pathogen is L *** assays using specific primers are very accurate and more sensitive and specific than microscopy which is time consuming and needs master mieroscopists.

关键词： Zoonotic Cutaneous Leishmaniasis Leishmania major Nested PCR Jask Iran

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Clinical and molecular features of human cystic echinococcosis in Tehran,Iran,2011-2019

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Asian Pacific Journal of Tropical Medicine 2022年第12期15卷 558-567页

作者： Narges Amani Hamedani Majid Pirestani Abdolhossein Dalimi Parasitology&entomology Department Faculty of Medical SciencesTarbiat Modares UniversityTehranIran

Objective:To evaluate the clinical and molecular features of human cystic echinococcosis in Tehran,***:In this cross-sectional study,all human cystic echinococcosis patients admitted to Tehran’s hospitals from 2011 to 2019 were enrolled,and demographic characteristics,clinical findings,and laboratory data were ***,paraffin-embedded and fresh tissue samples of 175 cystic echinococcosis patients were evaluated for molecular *** samples’isolated DNA was used to amplify cytochrome c oxidaseⅠand NADH dehydrogenase subunitⅠ***,the morphological features of fresh samples were ***:In total,175 patients with a mean age of 45(9-98)years were admitted to Tehran’s hospitals diagnosed with cystic ***,the highest(26.9%)and the lowest(2.9%)prevalence of cystic echinococcosis cases were in the 16-30 and 1-15 years range,***/female ratio was 0.96(49.1%vs.50.9%).The liver was affected in 92 patients(52.6%),and two or more organs were infected in 7 patients(4.0%).The cysts’diameter varied from 1 to 25 cm,and 96.0%of the patients had a single hydatid *** patients underwent radical surgery and the PAIR technique was applied for all *** significant difference was observed between the protoscolex hooks of pulmonary and hepatic cysts.G1-G3 had the highest percentage(99.4%)over other identified G6 genotypes(0.6%).Conclusions:The sheep-dog cycle plays an important role in transmitting the human cystic echinococcosis infection in Tehran.

关键词： Human cystic echinococcosis Clinical features Genotype Tehran

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The evolution of Hessian fly from the Old World to the New World： Evidence from molecular markers

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Insect Science 2015年第6期22卷 768-784页

作者： Brandon J. Schemerhom Yan Ma Crane Charles F. Crane USDA-ARS Department of entomology Department of Botany and Plant Pathology Purdue University West Lafayette IN 47907USA

Eighteen polymorphic microsatellite loci and 11 single-nucleotide polymor- phisms were genotyped in 1 095 individual Hessian fly specimens representing 23 popula- tions from North America, southern Europe, and southwest Asia. The genotypes were used to assess genetic diversity and interrelationship of Hessian fly populations. While phyloge- netic analysis indicates that the American populations most similar to Eurasian populations come from the east coast of the United States, genetic distance is least between （Alabama and California） and （Kazakhstan and Spain）. Allelic diversity and frequency vary across North America, but they are not correlated with distance from the historically documented point of introduction in New York City or with temperature or precipitation. Instead, the greatest allelic diversity mostly occurs in areas with Mediterranean climates. The mi- crosatellite data indicate a general deficiency for heterozygotes in Hessian fly. The North American population structure is consistent with multiple introductions, isolation by dis- tance, and human-abetted dispersal by bulk transport of puparia in infested straw or on harvesting equipment.

关键词： allele frequency Hessian fly microsatellite population structure SNP SSR

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Termites and Chinese agricultural system: applications and advances in integrated termite management and chemical control

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Insect Science 2021年第1期28卷 2-20页

作者： Farhan Ahmad Hatem Fouad Shi-You Liang Yin Hu Jian-Chu Mo Ministry of Agriculture Key Lab of molecular Biology of Crop Pathogens and Insect Pests Institute of Insect SciencesCollege of Agricultural and BiotechnologyZhejiang UniversityHangzhouChina entomology Section Central Cotton Research InstituteSakrandShaheed BenazirabadSindhPakistan Department of Field Crop Pests Plant Protection Research InstituteAgricultural Research CentreCairoEgypt National Termite Control Center HangzhouChina Ministry of Agriculture Key laboratory of molecular Biology of Crop Pathogens and Insects Institute of Insect SciencesCollege of Agriculture and BiotechnologyZhejiang UniversityYuhangtang Road 866Hangzhou 310058China

Termites are eusocial arthropod decomposers,and improve soil fertility,crop yield,and also are used by humans for their ben efits across the ***,some species of termites are becoming a threat to the farming community as they are directly and indirectly causing major losses to the agricultural *** is estimated that termites cost the global economy more than 40 billion USD annually,and considerable research has been done on their *** this review,we present the available information related to sustainable and integrated termite management practices(ITM).Furthermore,we insist that the better management of this menace can be possible through:(i)improving traditional methods to keep termites away from crops;(ii)improving agricultural practices to maintain plants with more vigor and less susceptible to termite attack;and(iii)integration of available techniques to reduce termite infestation in crops and *** application of an effective combination of traditional practices with recently developed approaches is the best option for agricultural ***,keeping in mind the beneficial nature of this pest,more innovative efforts for its management,particularly using rapidly emerging technology(e.g.,RNA interference),are needed.

关键词： entomopathogens geographical distribution integrated termite management termite

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Identification and Expression of a b-actin Gene from Liposcelis bostrychophila Badonnel(Psocoptera:Liposcelididae)

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Agricultural Sciences in China 2011年第9期10卷 1391-1401页

作者： JIANG Hong-bo SHEN Guang-mao DOU Wei TANG Pei-an LIU Yong-hua ZHOU An-wei WANG Jin-jun Key laboratory of entomology and Pest Control Engineering College of Plant Protection Southwest University Chongqing 400716 P. R. China College of Food Science and Engineering Nanjing University of Finance and Economics Nanjing 210003 P.R. China

A β-actin gene, Libβ-actinl, from the psocid, Liposcelis bostrychophila, was isolated, sequenced, and expressed in Escherichia coll. The cDNA sequence was 1 281 bp in length and contained an open reading frame of 1 131 bp encoding 376 amino acids with a predicted molecular weight of 41.82 kDa. According to a B lastN search, the coding region shared the highest identity （97%） with Pediculus hurnanus actin 5C, while the deduced amino acid sequence was completely identical to a mutant of Drosophila melanogaster actin 5C. Comparison of the nucleotide and deduced amino acid sequences confirmed the high similarity between Libβ-actinl and homologs in other insect species. The 3＇ untranslated region （3＇ UTR） of the Libβ-actinl mRNA had a high A＋U content （approximately 75%） and contained three repeats of the AUUUUUA and AUUUA motifs, which may play a role in regulating mRNA decay. The expression of Libβ-actinl was further analyzed in insecticide induced and control psocids. The results indicated that there was no significant difference in expression of Libβ-actinl between the induced and control groups, suggesting that Libβ-actinl may be an appropriate internal control for the gene expression profiling in this insect. Furthermore, Libβ-actinl was also heterologously expressed in Escherichia coli, which provided a basis to investigate the physiological functions of actin genes in the psocid.

关键词： actin sequence analysis expression real-time PCR

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Changes in Growth,Photosynthetic Pigments,Cell Viability,Lipid Peroxidation and Antioxidant Defense System in Two Varieties of Chickpea(Cicer arietinum L.)Subjected to Salinity Stress

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Phyton-International Journal of Experimental Botany 2022年第1期91卷 149-168页

作者： Zeenat Mushtaq Shahla Faizan Basit Gulzar Humira Mushtaq Sayyada Bushra Alisha Hussain Khalid Rehman Hakeem Environmental Physiology laboratory Department of BotanyAligarh Muslim UniversityAligarh202002India Cellular Differentiation and molecular Genetics Section Department of BotanyJamia HamdardNew Delhi110062India Research and Training Centre for Pollinators Pollinizers and Pollination Management SectionDivision of EntomologySKUAST KashmirSrinagar190025India Department of Biological Sciences Faculty of ScienceKing Abdulaziz UniversityJeddah21589Saudi Arabia

Salinity is one of the most severe abiotic stresses for crop *** present study investigates the salinityinduced modulation in growth indicators,morphology and movement of stomata,photosynthetic pigments,activity of carbonic anhydrase as well as nitrate reductase,and antioxidant systems in two varieties of chickpea(Pusa-BG5023,and Pusa-BGD72).On 20^(th) day of sowing,plants were treated with varying levels of NaCl(0,50,100,150 and 200 mM)followed by sampling on 45 days of *** observations on both the varieties reveal that salt stress leads to a significant decline in growth,dry biomass,leaf area,photosynthetic pigments,protein content,stomatal behavior,cell viability,activity of nitrate reductase and carbonic anhydrase with the rise in the concentration of ***,quantitatively these changes were less in Pusa-BG5023 as compared to ***,salinity-induced oxidative stress enhanced malondialdehyde content,superoxide radicals,foliar proline content,and the enzymatic activities of superoxide dismutase,catalase,and *** variety Pusa-BGD72 was found more sensitive than Pusa-BG5023 to salt *** of different graded concentrations(50,100,150 and 200 mM)of sodium chloride,50 mM was least toxic,and 200 mM was most *** differential behavior of these two varieties measured in terms of stomatal behavior,cell viability,photosynthetic pigments,and antioxidant defense system can be used as prospective indicators for selection of chickpea plants for salt tolerance and sensitivity.

关键词： Salt stress ion homeostasis antioxidant defense cell viability chickpea

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