检索结果-南通市图书馆

Global gene expression profiling of blast lung injury of goats exposed to shock wave

Chinese Journal of Traumatology 2020年第5期23卷 249-257页

作者： Hong Wang Wen-Juan Zhang Jun-Hong Gao Jin-Ren Liu Zhi-Yong Liu Bao-Qing Xia Xiao-Lin Fan Cun-Zhi Li Ai-Rong Qian Lab for bone metabolism Xi'an Key Laboratory of Special Medicine and Health EngineeringKey Lab for Space Biosciences and BiotechnologyResearch Center for Special Medicine and Health Systems EngineeringNPU-UAB Joint Laboratory for Bone MetabolismSchool of Life SciencesNorthwestern Polytechnical UniversityXi'an 710072China Research Center for Toxicological and Biological Effects Institute for Hygiene of Ordnance IndustryXi'an 710065China

Purpose:Blast lung injury(BLI)is the most common damage resulted from explosion-derived shock wave in military,terrorism and industrial ***,the molecular mechanisms underlying BLI induced by shock wave are still ***:In this study,a goat BLI model was established by a fuel air explosive *** key genes involved in were *** goats of the experimental group were fixed on the edge of the explosion cloud,while the goats of the control group were 3 km far away from the explosive *** successful modeling for 24 h,all the goats were sacrificed and the lung tissue was harvested for histopathological observation and RNA *** ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)analysis were performed to identify the main enriched biological functions of differentially expressed genes(DEGs).Quantitative real-time polymerase chain reaction(qRT-PCR)was used to verify the consistency of gene ***:Of the sampled goat lungs,895 genes were identified to be significantly differentially expressed,and they were involved in 52 significantly enriched GO *** analysis revealed that DEGs were highly enriched in 26 pathways,such as cytokine-cytokine receptor interaction,antifolate resistance,arachidonic acid metabolism,amoebiasis and bile secretion,JAK-STAT,and IL-17 signaling ***,15 key DEGs involved in the biological processes of BLI were confirmed by qRTPCR,and the results were consistent with RNA ***:Gene expression profiling provide a better understanding of the molecular mechanisms of BLI,which will help to set strategy for treating lung injury and preventing secondary lung injury induced by shock wave.

关键词： Blast lung injury Shock wave Differentially expressed genes RNA sequencing Transcriptome

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Skeletal phenotypes in secreted frizzled-related protein 4 gene knockout mice mimic skeletal architectural abnormalities in subjects with Pyle's disease from SFRP4 mutations

引用

bone Research 2023年第1期11卷 193-200页

作者： Robert Brommage Jeff Liu David R.Powell Department of metabolism Research Lexicon PharmaceuticalsThe WoodlandsTX77381USA

Mutations in SFRP4 cause Pyle’s bone disease with wide metaphyses and increased skeletal *** WNT signaling pathway plays important roles in determining skeletal architecture and SFRP4 is a secreted Frizzled decoy receptor that inhibits WNT *** cohorts of male and female Sfrp4 gene knockout mice,examined through 2 years of age,had a normal lifespan but showed cortical and trabecular bone *** human Erlenmeyer flask deformities,bone cross-sectional areas were elevated 2-fold in the distal femur and proximal tibia but only 30%in femur and tibia *** cortical bone thickness was observed in the vertebral body,midshaft femur and distal *** trabecular bone mass and numbers were observed in the vertebral body,distal femur metaphysis and proximal tibia *** femurs retained extensive trabecular bone through 2 years of *** bodies had increased compressive strength,but femur shafts had reduced bending ***,but not cortical,bone parameters in heterozygous Sfrp4mice were modestly *** resulted in similar declines in both cortical and trabecular bone mass in wild-type and Sfrp4 KO ***4 is critical for metaphyseal bone modeling involved in determining bone ***4 KO mice show similar skeletal architecture and bone fragility deficits observed in patients with Pyle’s disease with SFRP4 mutations.

关键词： SFRP4 skeletal elevated

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Bioengineered miR-27b-3p and miR-328-3p modulate drug metabolism and disposition via the regulation of target ADME gene expression

引用

Acta Pharmaceutica Sinica B 2019年第3期9卷 639-647页

作者： Xin Li Ye Tian Mei-Juan Tu Pui Yan Ho Neelu Batra Ai-Ming Yu Key laboratory of Molecular Target&Clinical Pharmacology School of Pharmaceutical Sciences&the Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou511436China Lab for bone metabolism Key Lab for Space Biosciences and BiotechnologySchool of Life SciencesNorthwestern PolytechnicalUniversityXi'an710072China Department of Biochemistry&Molecular Medicine UC Davis School of MedicineSacramentoCA95817USA

Drug-metabolizing enzymes, transporters, and nuclear receptors are essential for the absorption, distribution, metabolism, and excretion(ADME) of drugs and xenobiotics. MicroRNAs participate in the regulation of ADME gene expression via imperfect complementary Watson–Crick base pairings with target transcripts. We have previously reported that Cytochrome P450 3A4(CYP3A4) and ATP-binding cassette sub-family G member 2(ABCG2) are regulated by miR-27b-3p and miR-328-3p,respectively. Here we employed our newly established RNA bioengineering technology to produce bioengineered RNA agents(BERA), namely BERA/miR-27b-3p and BERA/miR-328-3p, via fermentation. When introduced into human cells, BERA/miR-27b-3p and BERA/miR-328-3p were selectively processed to target miRNAs and thus knock down CYP3A4 and ABCG2 mRNA and their protein levels,respectively, as compared to cells treated with vehicle or control RNA. Consequently, BERA/miR-27b-3p led to a lower midazolam 10-hydroxylase activity, indicating the reduction of CYP3A4 activity. Likewise,BERA/miR-328-3p treatment elevated the intracellular accumulation of anticancer drug mitoxantrone, a classic substrate of ABCG2, hence sensitized the cells to chemotherapy. The results indicate that biologic miRNA agents made by RNA biotechnology may be applied to research on miRNA functions in the regulation of drug metabolism and disposition that could provide insights into the development of more effective therapies.

关键词： Bioengineered RNA miR-27b miR-328 CYP3A4 ABCG2 Drug disposition

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of cyclic mechanical loading on immunoinflammatory microenvironment in biofabricating hydroxyapatite scaffold for bone regeneration

引用

Bioactive Materials 2021年第10期6卷 3097-3108页

作者： Penghui Zhang Xizhe Liu Peng Guo Xianlong Li Zhongyuan He Zhen Li Martin J.Stoddart Sibylle Grad Wei Tian Dafu Chen Xuenong Zou Zhiyu Zhou Shaoyu Liu Innovation Platform of Regeneration and Repair of Spinal Cord and Nerve Injury Department of Orthopaedic SurgeryThe Seventh Affiliated HospitalSun Yat-sen UniversityShenzhen518107China Guangdong Provincial Key laboratory of Orthopedics and Traumatology Orthopaedic Research Institute/Department of Spinal SurgeryThe First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou510080China AO Research Institute Davos Clavadelerstrasse 8Davos7270Switzerland laboratory of bone Tissue Engineering Beijing Laboratory of Biomedical MaterialsBeijing Research Institute of Orthopaedics and TraumatologyBeijing JiShuiTan HospitalBeijing100035China

It has been proven that the mechanical microenvironment can impact the differentiation of mesenchymal stem cells(MSCs).However,the effect of mechanical stimuli in biofabricating hydroxyapatite scaffolds on the inflammatory response of MSCs remains *** study aimed to investigate the effect of mechanical loading on the inflammatory response of MSCs seeded on *** mechanical loading was applied to biofabricate the cell-scaffold composite for 15 min/day over 7,14,or 21 *** the predetermined time points,culture supernatant was collected for inflammatory mediator detection,and gene expression was analyzed by *** results showed that the expression of inflammatory mediators(IL1B and IL8)was downregulated(pbone volume/total volume ratio in ***,these findings demonstrated that mechanical loading could dually modulate the inflammatory responses and osteogenic differentiation of ***,the ECM-based scaffolds that were biofabricated with mechanical loading before freeze-drying facilitated the M2 polarization of macrophages in vitro and bone regeneration in *** loading may be a promising biofabrication strategy for bone biomaterials.

关键词： Bioreactor Biofabrication Inflammatory microenvironment bone biomaterials Macrophage polarization

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Enhanced osteogenesis of hydroxyapatite scaffolds by coating with BMP-2-loaded short polylactide nanofiber: a new drug loading method for porous scaffolds

引用

Regenerative Biomaterials 2020年第1期7卷 91-98页

作者： Taotao Xu Luyao Sheng Lei He Jie Weng Ke Duan Key laboratory of Advanced Technologies of Materials(MOE) School of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China Sichuan Provincial laboratory of Orthopaedic Engineering Department of Bone and Joint SurgeryAffiliated Hospital of Southwest Medical UniversityLuzhou 646000China

Porous hydroxyapatite(HA)is widely used in porous forms to assist bone defect ***,further improvements in biological functions are desired for meeting complex clinical situations such as impaired bone regeneration in poor bone *** extracellular matrix(ECM)of human tissues is characterized by nanofibrous structures and a variety of signal *** these characteristics are expected to create a favorable microenvironment for cells and simultaneously allow release of osteogenic *** this study,short polylactide fibers containing BMP-2 were prepared by electrospinning and coated on porous HA *** coating did not affect porosity or pore interconnectivity of the scaffold but improved its compressive strength *** fiber coating produced burst BMP-2 release in 1 day followed by a linear release for 24 *** coating had a significantly lower rat calvarial osteoblasts(RCOBs)adhesion(*** scaffold)but allowed normal proliferation *** marrow stem cells(MSCs)on the coated scaffolds expressed a significantly increased alkaline phosphatase activity relative to the uncoated *** implantation in canine dorsal muscles,the coated scaffolds formed significantly more new bone at Weeks 4 and 12,and more blood vessels at Week *** method offers a new option for drug delivery systems.

关键词： short fibers surface modification drug release hydroxyapatite polylactide

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

In Situ Sustained Macrophage-Targeted Nanomicelle-Hydrogel Microspheres for Inhibiting Osteoarthritis

引用

Research 2024年第1期2023卷 1-16页

作者： XiaoXiao Li Xingchen Li Jielai Yang Yawei Du Liang Chen Gang Zhao Tingjun Ye Yuan Zhu Xiangyang Xu Lianfu Deng Wenguo Cui Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint DiseasesShanghai Institute of Traumatology and OrthopaedicsRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025P.R.China

There are stillchallenges in applying drug nanocarriers for in situ sustained macrophage targeting and regulation,due to the rapid clearance of nanocarriers and burst drug release ***,a nanomicellehydrogel microsphere,characterized by its macrophage-targeted nanosized secondary structure that allows it to accurately bind to M1 macrophages through active endocytosis,is employed for in situ sustained macrophage targeting and regulation,and addresses the insufficient osteoarthritis therapeutic efficacy caused by rapid clearance of drug *** 3-dimensional structure of a microsphere can prevent the rapid escape and clearance of a nanomicelle,thus keeping it in joints,while the ligand-guided secondary structure can carry drugs to accurately target and enter M1 macrophages,and release drugs via the transition from hydrophobicity to hydrophilicity of nanomicelles under inflammatory stimulation inside the macrophages.

关键词： micelle release spheres

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Rapid Communication:Preliminary study on the freeze-drying of human bone marrow-derived mesenchymal stem cells

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第11期11卷 889-894页

作者： Shao-zhi ZHANG Huan QIAN Zhen WANG Ju-li FAN Qian ZHOU Guang-ming CHEN Rui LI Shan FU Jie SUN Refrigeration and Cryogenic Engineering lnstitute Zhejiang University Hangzhou 310027 China bone Marrow Transplantation Center the First Affiliate Hospital School of Medicine Zhejiang University Hangzhou 310003 China

Long-term preservation and easy transportation of human bone marrow-derived mesenchymal stem cells(hBM-MSCs) will facilitate their application in medical treatment and bioengineering.A pilot study on the freeze-drying of hBM-MSCs was carried ***-MSCs were loaded with *** glass transition temperature of the freeze-drying suspension was measured to provide information for the cooling and primary drying *** freeze-drying,various rehydration processes were *** highest recovery rate of hBM-MSCs was(69.33± 13.08) %.Possible methods to improve freeze-drying outcomes are *** conclusion,the present study has laid a foundation for the freeze-drying hBM-MSCs.

关键词： Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) Freeze-drying Trehalose Rehydration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Unveiling the role of hypoxia-inducible factor 2alpha in osteoporosis:Implications for bone health

引用

World Journal of Stem Cells 2024年第4期16卷 389-409页

作者： Ling-Ling Wang Zhan-Jin Lu Shun-Kui Luo Yun Li Zhe Yang Hong-Yun Lu Department of Gerontology The Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhai 519000Guangdong ProvinceChina Department of Endocrinology and metabolism The Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhai 519000Guangdong ProvinceChina Guangdong Provincial Key laboratory of Biomedical Imaging The Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhai 519000Guangdong ProvinceChina Department of Endocrinology and metabolism Zhuhai People’s Hospital(Zhuhai Clinical Medical College of Jinan Universitythe First Hospital Affiliated with Medical College of Macao University of Science and Technology)Zhuhai 519000Guangdong ProvinceChina

BACKGROUND Osteoporosis(OP)has become a major public health problem *** OP treatments are based on the inhibition of bone resorption,and it is necessary to identify additional treatments aimed at enhancing *** the bone marrow(BM)niche,bone mesenchymal stem cells(BMSCs)are exposed to a hypoxic ***,a few studies have demonstrated that hypoxiainducible factor 2alpha(HIF-2α)is involved in BMSC osteogenic differentiation,but the molecular mechanism involved has not been *** To investigate the effect of HIF-2αon the osteogenic and adipogenic differentiation of BMSCs and the hematopoietic function of hematopoietic stem cells(HSCs)in the BM niche on the progression of *** Mice with BMSC-specific HIF-2αknockout(Prx1-Cre;Hif-2αfl/fl mice)were used for in vivo *** quantification was performed on mice of two genotypes with three interventions:Bilateral ovariectomy,semilethal irradiation,and dexamethasone ***,the hematopoietic function of HSCs in the BM niche was compared between the two mouse *** vitro,the HIF-2αagonist roxadustat and the HIF-2αinhibitor PT2399 were used to investigate the function of HIF-2αin BMSC osteogenic and adipogenic ***,we investigated the effect of HIF-2αon BMSCs via treatment with the mechanistic target of rapamycin(mTOR)agonist MHY1485 and the mTOR inhibitor *** The quantitative index determined by microcomputed tomography indicated that the femoral bone density of Prx1-Cre;Hif-2αfl/fl mice was lower than that of Hif-2αfl/fl mice under the three intervention *** vitro,Hif-2αfl/fl mouse BMSCs were cultured and treated with the HIF-2αagonist roxadustat,and after 7 d of BMSC adipogenic differentiation,the oil red O staining intensity and mRNA expression levels of adipogenesis-related genes in BMSCs treated with roxadustat were decreased;in addition,after 14 d of osteogenic differentiation,BMSCs treated with roxadusta

关键词： Hypoxia-inducible factor-2α bone marrow niche bone mesenchymal stem cells Osteoporosis Osteogenic/adipogenic differentiation Mechanistic target of rapamycin signaling pathway

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Double crosslinked biomimetic composite hydrogels containing topographical cues and WAY-316606 induce neural tissue regeneration and functional recovery after spinal cord injury

引用

Bioactive Materials 2023年第6期24卷 331-345页

作者： Xingchang Zhao Xianzhe Lu Kai Li Shiqiang Song Zhaohui Luo Chuanchuan Zheng Chengliang Yang Xiumei Wang Liqiang Wang Yujin Tang Chong Wang Jia Liu Department of Orthopaedics Affiliated Hospital of Youjiang Medical University for NationalitiesBaiseGuangxiChina Guangxi Key laboratory of Basic and Translational Research of bone and Joint Degenerative Diseases Guangxi Biomedical Materials Engineering Research Center for Bone and Joint Degenerative DiseasesBaiseGuangxiChina Academy of Orthopedics Guangdong ProvinceGuangdong Provincial Key Laboratory of Bone and Joint Degeneration DiseasesThe Third Affiliated Hospital of Southern Medical UniversityGuangzhouChina Youjiang Medical University for Nationalities BaiseGuangxiChina School of Mechanical Engineering Dongguan University of TechnologySongshan LakeDongguanGuangdongChina State Key laboratory of New Ceramics and Fine Processing Key Laboratory of Advanced Materials of Ministry of EducationSchool of Materials Science and EngineeringTsinghua UniversityBeijingChina State Key laboratory of Metal Matrix Composites School of Material Science and EngineeringShanghai Jiao Tong UniversityShanghaiChina

Spinal cord injury(SCI)is an overwhelming and incurable disabling condition,for which increasing forms of multifunctional biomaterials are being tested,but with limited *** promising material should be able to fill SCI-induced cavities and direct the growth of new neurons,with effective drug loading to improve the local micro-organism environment and promote neural tissue *** this study,a double crosslinked biomimetic composite hydrogel comprised of acellularized spinal cord matrix(ASCM)and gelatin-acrylated-β-cyclodextrin-polyethene glycol diacrylate(designated G-CD-PEGDA)hydrogel,loaded with WAY-316606 to activate canonical Wnt/β-catenin signaling,and reinforced by a bundle of three-dimensionally printed aligned polycaprolactone(PCL)microfibers,was *** G-CD-PEGDA component endowed the composite hydrogel with a dynamic structure with a self-healing capability which enabled cell migration,while the ASCM component promoted neural cell affinity and *** diffusion of WAY-316606 could recruit endogenous neural stem cells and improve neuronal *** aligned PCL microfibers guided neurite elongation in the longitudinal *** behavior studies further showed that the composite hydrogel could significantly recover the motor function of rats after *** study provides a proficient approach to produce a multifunctional system with desirable physiological,chemical,and topographical cues for treating patients with SCI.

关键词： Spinal cord injury 3D printing PCL oriented fibers WAY-316606 Wnt/β-catenin signaling

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Carboxymethyl chitosan-alginate enhances bone repair effects of magnesium phosphate bone cement by activating the FAK-Wnt pathway

引用

Bioactive Materials 2023年第2期20卷 598-609页

作者： Ling Yu Tian Gao Wei Li Jian Yang Yinchu Liu Yanan Zhao Ping He Xuefeng Li Weichun Guo Zhengfu Fan Honglian Dai Department of Orthopedic Surgery I(Spine Surgery) Renmin Hospital of Wuhan UniversityWuhan430060China Department of bone and Soft Tissue Tumor Key Laboratory of Carcinogenesis and Translational ResearchMinistry of EducationPeking University Cancer Hospital&InstituteBeijing100000China State Key laboratory of Advanced Technology for Materials Synthesis and Processing Biomedical Materials and Engineering Research Center of Hubei ProvinceWuhan University of TechnologyWuhan430070China Department of Orthopedic Surgery Renmin Hospital of ShayangJingmen448200China

There is a continuing need for artificial bone substitutes for bone repair and reconstruction,Magnesium phosphate bone cement(MPC)has exceptional degradable properties and exhibits promising ***,its mechanical strength needs improved and its low osteo-inductive potential limits its therapeutic application in bone *** functionally modified MPC by using a polymeric carboxymethyl chitosan-sodium alginate(CMCS/SA)gel *** had the advantages of:improved compressive strength,ease of handling,and an optimized interface for bioactive bone *** new composites with 2%CMCS/SA showed the most favorable physicochemical properties,including mechanical strength,wash-out resistance,setting time,injectable time and heat ***,the composite promoted the attachment and proliferation of osteoblast *** was also found to induce osteogenic differentiation in vitro,as verified by expression of osteogenic *** terms of molecular mechanisms,data showed that new bone cement activated the Wnt pathway through inhibition of the phosphorylation ofβ-catenin,which is dependent on focal adhesion *** micro-computed tomography and histological analysis,we found that the MPC-CMCS/SA scaffolds,compared with MPC alone,showed increased bone regeneration in a rat calvarial defect ***,our study suggested that the novel composite had potential to help repair critical bone defects in clinical practice.

关键词： Critical bone defect Magnesium phosphate cement Carboxymethyl chitosan Sodium alginate Osteogenic differentiation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：