Global gene expression profiling of blast lung injury of goats exposed to shock wave

作     者：Hong Wang Wen-Juan Zhang Jun-Hong Gao Jin-Ren Liu Zhi-Yong Liu Bao-Qing Xia Xiao-Lin Fan Cun-Zhi Li Ai-Rong Qian Wang Hong;Zhang Wen-Juan;Gao Jun-Hong;Liu Jin-Ren;Liu Zhi-Yong;Xia Bao-Qing;Fan Xiao-Lin;Li Cun-Zhi;Qian Ai-Rong

作者机构：Lab for Bone MetabolismXi'an Key Laboratory of Special Medicine and Health EngineeringKey Lab for Space Biosciences and BiotechnologyResearch Center for Special Medicine and Health Systems EngineeringNPU-UAB Joint Laboratory for Bone MetabolismSchool of Life SciencesNorthwestern Polytechnical UniversityXi'an 710072China Research Center for Toxicological and Biological EffectsInstitute for Hygiene of Ordnance IndustryXi'an 710065China 

出 版 物：《Chinese Journal of Traumatology》 (中华创伤杂志（英文版）)

年 卷 期：2020年第23卷第5期

页      面：249-257页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：This work was supported by Science and Technology Development Fund for Institute for Hygiene of Ordnance(KY202007) 

主　　题：Blast lung injury Shock wave Differentially expressed genes RNA sequencing Transcriptome 

摘      要：Purpose:Blast lung injury(BLI)is the most common damage resulted from explosion-derived shock wave in military,terrorism and industrial ***,the molecular mechanisms underlying BLI induced by shock wave are still ***:In this study,a goat BLI model was established by a fuel air explosive *** key genes involved in were *** goats of the experimental group were fixed on the edge of the explosion cloud,while the goats of the control group were 3 km far away from the explosive *** successful modeling for 24 h,all the goats were sacrificed and the lung tissue was harvested for histopathological observation and RNA *** ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)analysis were performed to identify the main enriched biological functions of differentially expressed genes(DEGs).Quantitative real-time polymerase chain reaction(qRT-PCR)was used to verify the consistency of gene ***:Of the sampled goat lungs,895 genes were identified to be significantly differentially expressed,and they were involved in 52 significantly enriched GO *** analysis revealed that DEGs were highly enriched in 26 pathways,such as cytokine-cytokine receptor interaction,antifolate resistance,arachidonic acid metabolism,amoebiasis and bile secretion,JAK-STAT,and IL-17 signaling ***,15 key DEGs involved in the biological processes of BLI were confirmed by qRTPCR,and the results were consistent with RNA ***:Gene expression profiling provide a better understanding of the molecular mechanisms of BLI,which will help to set strategy for treating lung injury and preventing secondary lung injury induced by shock wave.