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Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fraction

Journal of Animal Science and Biotechnology 2024年第1期15卷 171-181页

作者： Estel Viñolas-Vergés Jordi Ribas-Maynou Isabel Barranco Camila Peres Rubio Sergi Bonet Jordi Roca Marc Yeste Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaGironaSpain Biotechnology of Animal and Human Reproduction(TechnoSperm) Institute of Food and Agricultural TechnologyUniversity of GironaGironaSpain Department of Medicine and Animal surgery Faculty of Veterinary ScienceUniversity of MurciaMurciaSpain Catalan Institution for Research and Advanced Studies(ICREA) S08010 BarcelonaSpain

Background Protamination and condensation of sperm chromatin as well as DNA integrity play an essential role during fertilization and embryo *** some mammals,like pigs,ejaculates are emitted in three separate fractions:pre-sperm,sperm-rich(SRF)and post sperm-rich(PSRF).These fractions are known to vary in volume,sperm concentration and quality,as well as in the origin and composition of seminal plasma(SP),with differences being also observed within the SRF ***,whether disparities in the DNA integrity and chromatin condensation and pro-tamination of their sperm exist has not been *** This study determined chromatin protamination(Chromomycin A3 test,CMA_(3)),condensation(Dibromobi-mane test,DBB),and DNA integrity(Comet assay)in the pig sperm contained in the first 10 m L of the SRF(SRF-P1),the remaining portion of the sperm-rich fraction(SRF-P2),and the post sperm-rich fraction(PSRF).While chromatin protamination was found to be similar between the different ejaculate fractions(P>0.05),chromatin condensation was seen to be greater in SRF-P1 and SRF-P2 than in the PSRF(P=0.018 and P=0.004,respectively).Regarding DNA integrity,no differences between fractions were observed(P>0.05).As the SRF-P1 has the highest sperm concentra-tion and ejaculate fractions are known to differ in antioxidant composition,the oxidative stress index(OSi)in SP,calcu-lated as total oxidant activity divided by total antioxidant capacity,was tested and confirmed to be higher in the SRF-P1 than in SRF-P2 and PSRF(0.42±0.06 vs.0.23±0.09 and 0.08±0.00,respectively;P<0.01);this index,in addition,was observed to be correlated to the sperm concentration of each fraction(Rs=0.973;P<0.001).Conclusion While sperm DNA integrity was not found to differ between ejaculate fractions,SRF-P1 and SRF-P2 were observed to exhibit greater chromatin condensation than the *** could be related to the OSi of each fraction.

关键词： Chromatin Condensation DNA integrity Ejaculate fractions Pig Protamination Sperm

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Betulinic acid protects against ovarian impairment by decreasing F-2 toxin-induced oxidative stress and inflammation associated with the downregulation of p38 expression in mice

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Food Science and Human Wellness 2024年第3期13卷 1292-1302页

作者： Li Kong Xinyu Gao Lijuan Zhu Xing Lin You Huang Chunlin Huang Wenjiang Yang Yazhi Chen Haoqiang Zhao Jing Wu Zhihang Yuan Jin’e Yi Hunan Engineering Research Center of Livestock and Poultry Health Care College of Veterinary MedicineHunan Agricultural UniversityChangsha 410128China

F-2 toxin is an estrogenic mycotoxin that causes reproductive disorders in *** acid(BA)is a natural pentacyclic lupane-structure triterpenoid that has diverse pharmacological *** this study,the antioxidative and anti-inflammatory effects of BA and its underlying mechanism are explored in F-2 toxin-triggered mouse ovarian *** found that BA alleviated the F-2 toxin-induced ovarian impairment by stimulating follicle growth,reducing inflammatory cell infiltration,repairing damaged mitochondria and endoplasmic ***,BA not only reversed F-2 toxin-induced reduction of follicle stimulating hormone(FSH)and luteinizing hormone(LH)levels in the serum,but also restrained the protein expression of the estrogen receptors a(ERa)and ERβ.Moreover,BA restored the balance of F-2 toxin-induced ovarian redox system ***,we found that 0.25 mg/kg BA played an anti-inflammatory role in the F-2 toxin-induced ovarian impairment by decreasing interleukin-1β(IL-1β).IL-6,and tumor necrosis factor-α(TNF-α)mRNA expression,as well as inhibiting p38 protein *** data demonstrated that BA exerts its protective effect on F-2 toxin-induced ovarian oxidative impairment and inflammation by inhibiting p38 expression,which implies a natural product-based medicine to ameliorate F-2 toxin-caused female reproductive toxicity and provides a detoxifying method for food contaminated by mycotoxin.

关键词： Betulinic acid F-2 toxin Ovarian damage p38 MAPK signaling pathway

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Effects of Moringa oleifera aqueous extract on the growth performance,blood characteristics,and histological features of gills and livers in Nile tilapia

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Aquaculture and Fisheries 2024年第1期9卷 85-92页

作者： Mahamoud A.Emam Ramy M.Shourbela Waleed N.El-Hawarry Seham Y.Abo-Kora Fatma Abdel-Monem Gad Ashraf M.Abd El-latif Mahmoud A.O.Dawood Histology Department Faculty of Veterinary MedicineBenha UniversityBenha13511Egypt Department of Animal Husbandry and Animal Wealth Development Faculty of Veterinary MedicineAlexandria UniversityAlexandria22758Egypt Pharmacology Department Faculty of Veterinary MedicineBenha UniversityBenha13511Egypt Clinical Pathology Department Faculty of Veterinary MedicineBenha UniversityBenha13511Egypt Department of Aquatic Animal Diseases and Management Faculty of Veterinary MedicineBenha UniversityBenha13511Egypt Animal Production Department Faculty of AgricultureKafrelsheikh UniversityKafr El-Sheikh33511Egypt The Center for Applied Research on the Environment and Sustainability The American University in CairoCairo11835Egypt

Moringa oleifera is well known as a highly nutritious plant and a water purifier in fish *** fish has many impressive qualities making them very suitable for ***,this study aimed to evaluate the effect of supplementation of Moringa oleifera aqueous extract(MOAE)at varying concentrations(0,100,200,and 400 mg/kg diet for 90 days)on growth performance,hematological,and some biochemical parameters of Nile ***,histological changes and immunohistochemical expression of caspase 3 in gills and livers were *** enhancements(P<0.05)in the final weight,weight gain,specific growth rate,red blood cells,hemoglobin,packed cell volume,white blood cells,and total serum protein were observed in fish fed 200 mg MOAE/kg diet compared with other ***,a significant reduction(P<0.05)of liver and kidney function tests and non-significant changes in lipid profile were *** the other hand,fish fed 400 mg MOAE/kg diet revealed disturbance in the corresponding *** histological degenerative changes were detected in the gills and liver of fish fed 400 mg MOAE/kg *** comparison,fish fed 200 mg MOAE/kg diet showed the best histo-architectures of these organs compared with other *** immune expressions of caspase 3 were determined where gills and liver of fish fed 400 mg MOAE/kg diet revealed extensive number of positive caspase 3 cells indicating severe apoptotic ***,a dietary MOAE at 200 mg/kg diet is recommended to improve the growth performance,hematological parameters,liver and kidney functions in Nile ***,caution must be considered when using MOAE at a concentration over 200 mg/kg diet.

关键词： Growth performance Liver Gills Moringa oleifera Nile tilapia

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Repeated inoculation with rumen fluid accelerates the rumen bacterial transition with no benefit on production performance in postpartum Holstein dairy cows

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Journal of Animal Science and Biotechnology 2024年第2期15卷 795-811页

作者： Fanlin Kong Feiran Wang Yijia Zhang Shuo Wang Wei Wang Shengli Li Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control State Key Laboratory of Animal NutritionDepartment of Animal Nutrition and Feed ScienceCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193PR China College of veterinary Medicine Nanjing Agricultural UniversityNanjingJiangsu 210095PR China

Background The dairy cow’s postpartum period is characterized by dramatic physiological changes,therefore imposing severe challenges on the animal for maintaining health and milk *** dynamics of the ruminal microbiota are also tremendous and may play a crucial role in lactation *** aim to investigate the potential benefits of early microbial intervention by fresh rumen microbiota transplantation(RMT)and sterile RMT in postpartum dairy *** fistulated peak-lactation dairy cows were selected to be the donors for rumen fluid *** postpartum cows were divided into 3 groups as the transplantation receptors respectively receiving 10 L fresh rumen fluid(FR),10 L sterile rumen fluid(SR),or 10 L saline(CON)during 3 d after *** Production performance,plasma indices,plasma lipidome,ruminal microbiome,and liver transcriptome were *** fresh and sterile RMT,we found that the molar proportion of propionic acid was increased on d 7 in the FR and SR groups and the bacterial composition was also significantly changed when compared with the CON group.A similarity analysis showed that the similarities between the CON group and FR or SR group on d 7 were 48.40%or 47.85%,whereas the similarities between microbiota on d 7 and 21 in the FR and SR groups were 68.34%or 66.85%.Dry matter intake and feed efficiency were not affected by ***β-hydroxybutyrate concentration in the FR group was decreased and significantly different lipids mainly included phosphatidylcholine and lysophosphatidylcholine containing polyunsaturated fatty *** transcriptomics analysis indicated acutephase response pathways were upregulated in the SR *** Our study suggests that RMT can shorten the transition process of the ruminal microbiota of postpartum dairy cows with no benefit on dry matter intake or feed *** with rumen fluid may not be a useful approach to promote the recovery of postpartum dairy cows.

关键词： Dairy cow Lipidomics Liver Rumen microbiota transplantation Transition period

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宿主蛋白TOB1通过IFN和EGFR通路影响口蹄疫病毒感染

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中国兽医科学 2024年第8期20卷 1136-1136页

作者： Gaochuang Peng Tianran Liu Xiaolan Qi Yuzhe Wang Jingjing Ren Jiangling Peng Xuguang Du Siyu Hu Sen Wu Yaofeng Zhao Dan Li Haixue Zheng State Key laboratory of Animal Biotech Breeding College of Biological Sciences National Engineering Laboratory for Animal Breeding Frontiers Science Center for Molecular Design Breeding China Agricultural University Beijing China State Key laboratory for Animal Disease Control and Prevention College of Veterinary Medicine Lanzhou University Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou China

口蹄疫病情（FMDV）在与宿主相互作用中进化出了一系列逃逸宿主抗病毒免疫的策略，在分子水平深入挖掘FMDV与宿主相互作用机制是高效疫苗研发和抗病分子育种的前提。2024年3月PLoS Pathogens杂志发表了“A genome-wide CRISPR screening... 详细信息

口蹄疫病情（FMDV）在与宿主相互作用中进化出了一系列逃逸宿主抗病毒免疫的策略，在分子水平深入挖掘FMDV与宿主相互作用机制是高效疫苗研发和抗病分子育种的前提。2024年3月PLoS Pathogens杂志发表了“A genome-wide CRISPR screening uncovers that TOB1acts as a key host factor for FMDV infection via both IFN and EGFR mediated pathways”的研究论文，论文的通讯作者为吴森教授、赵要风教授、李丹研究员、郑海学研究员。该研究利用CRISPR/Cas9敲除技术，在全基因组水平筛选了猪源细胞中与FMDV感染相关的重要宿主因子。

关键词： Foot and mouth disease Transfection Library screening Small interfering RNA EGFR signaling Polymerase chain reaction Plasmid construction Phosphorylation

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Detection of a reassortant swine H1N2 influenza A virus from pigs in Hong Kong

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Virologica Sinica 2024年第2期39卷 343-346页

作者： Guoqian Gu Congnuan Liu Song Hao Lee Lewis Sze Chun Choi Michael T.Wilson Dirk U.Pfeiffer Yun Young Go Department of Infectious Diseases and Public Health Jockey Club College of Veterinary Medicine and Life SciencesCity University of Hong KongHong Kong SARChina Center for Applied One Health Research and Policy Advice Jockey Club College of Veterinary Medicine and Life SciencesCity University of Hong KongHong Kong SARChina College of veterinary Medicine Konkuk UniversitySeoul143-701Republic of Korea

Dear Editor,Influenza A viruses(IAVs)are responsible for significant respiratory illnesses in humans and a broad range of animal *** outbreaks and the rapid spread of genetically and antigenically distinct IAVs represent a considerable challenge for swine *** are susceptible to avian and human IAV infections and are considered“mixing vessels”where human,avian,and swine IAVs can recombine and generate novel reassortant influenza viruses with pandemic potential(Vincent et al.,2014).

关键词： swine influenza mixing

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Ancylostoma ceylanicum and other zoonotic canine hookworms:neglected public and animal health risks in the Asia-Pacific region

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Animal Diseases 2024年第1期4卷 59-74页

作者： Jan Clyden B.Tenorio lan Kim B.Tabios Tawin Inpankaew Adrian P.Ybanez Saruda Tiwananthagorn Sirikachorn Tangkawattana Sutas Suttiprapa Tropical Medicine Graduate Program Faculty of MedicineKhon Kaen UniversityKhon Kaen 40002Thailand Department of Tropical Medicine Faculty of MedicineKhon Kaen UniversityKhon Kaen 40002Thailand WHO Collaborating Center for Research and Control of Opisthorchiasis(Southeast Asian Liver Fluke Disease) Tropical Disease Research CenterKhon Kaen UniversityKhon Kaen 40002Thailand Department of veterinary Paraclinical Sciences College of Veterinary MedicineUniversity of Southern MindanaoKabacan9407 CotabatoPhilippines Institute of Biology College of ScienceUniversity of the Philippines DilimanManila 1101Philippines Department of Parasitology Faculty of Veterinary MedicineKasetsart UniversityBangkok 10900Thailand College of veterinary Medicine at Barili Campus and Center for Vector-Borne and Protozoan Diseases at Main Campus Cebu Technological UniversityCebu City 6000Philippines Department of veterinary Biosciences and Public Health Faculty of Veterinary MedicineChiang Mai UniversityChiang Mai 50100Thailand Research Center for veterinary Biosciences and veterinary Public Health Chiang Mai UniversityChiang Mai 50100Thailand Faculty of veterinary Medicine Khon Kaen University.Khon Kaen 40002Thailand

Zoonotic hookworm infections remain a significant public health problem,causing nearly 500 milion cases globally and approximately four million disability-adjusted life years lost *** than one-fth of these cases are attrib-uted to Ancylostoma ceylanicum,an emerging zoonotic health issue in the Asia-Pacific *** review presents key research gaps regarding the epidemiology,diagnosis,control,prevention and elimination of *** and other canine zoonotic hookworms as neglected health *** is the second most prevalent human hook-worm in the region;it is the most common hookworm among dogs and cats-reservoirs of zoonotic *** population genetic and phylogenetic analyses revealed that *** has three possible transmis-sion dynamics:zoonotic,animal-only,and human-only *** actual burden of zoonotic ancylostomiasis in most endemic countries remains unknown due to the use of parasitological techniques(e.g.,Kato-Katz thick smear and floatation techniques)that have reduced diagnostic performance and do not allow accurate species identifica-tion in helminth *** emergence of benzimidazole resistance in soil-transmitted helminths(STHs),includ-ing hookworms,is a concern due to the protracted implementation of mass drug administration(MDA).Resistance is conferred by single nucleotide polymorphisms(SNPs)that occur in theβ-tubulin isotype 1 *** mutations have been reported in drug-resistant *** but have not been found in *** in the *** remains understudied in the Asia-Pacific *** zoonotic nature of the parasite warrants investigation of its occur-rence in human and animal reservoir hosts to understand the dynamics of zoonotic transmission in different endemic *** detection of benzimidazole resistance-associated SNPs in zoonotic hookworms from Asia-Pacific countries has yet to be thoroughly *** the high level of hookworm endemicity in the region

关键词： Soil-transmitted helminths Neglected tropical diseases Mass drug administration Deworming veterinary public health

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应对可转移多黏菌素耐药性--饲料添加剂来源的白屈菜红碱具有双重效应

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Engineering 2024年第1期32卷 163-173页

作者： Huangwei Song Xueyang Wang Muchen Zhang Zhiyu Zou Siyuan Yang Tian Yi Jianfeng Wang Dejun Liu Yingbo Shen Chongshan Dai Zhihai Liu Timothy R.Walsh Jianzhong Shen Congming Wu Yang Wang National Key laboratory of veterinary Public Health and Safety College ofVeterinary MedicineChina Agricultural UniversityBeijing 100193China Guangdong laboratory for Lingnan Modern Agriculture Guangzhou 510642China State Key laboratory for Zoonotic Diseases Key Laboratory of Zoonosis ResearchMinistry of EducationInstitute of ZoonosisCollege of Veterinary MedicineJilin UniversityChangchun 130015China College of Chemistry and Pharmaceutical Sciences Qingdao Agricultural University.Qingdao 266109China Ineos-Oxford Institute of Antimicrobial Research Department of BiologyUniversity of OxfordOxford OX13SZUK

The emergence and spread of the mobile colistin-resistance gene,mcr-1,and its variants pose achallenge to the use of colistin,a last-resort antibiotic used to treat severe infections caused by extensively drug-resistant(XDR)Gram-negative *** adjuvants are a promising strategy to enhance the efficacy of colistin against colistin-resistant pathogens;however,few studies have considered the effects of adjuvants on limiting resistance-gene *** found that chelerythrine(4 mg·L^(-1))derived from Macleaya cordata extract,which is used as an animal feed additive,reduced the minimal inhibitory concentration(MIC)of colistin against an mcr-1 positive Escherichia coli(***)strain by 16-fold(from 2.000 to 0.125 mg·L^(-1)).eliminated approximately 10^(4) colony-forming units(CFUs)of an mcr-1-carrying strain in a murine intestinal infection model,and inhibited the conjugation of an mcr-1-bearing plasmid in vitro(by>100-fold)and in a mouse model(by up to 5-fold).A detailed analysis revealed that chelery-thrine binds to phospholipids on bacterial membranes and increases cytoplasmic membrane fluidity,thereby impairing respiration,disrupting proton motive force(PMF),generating reactive oxygen species(ROS),and decreasing intracellular adenosine triphosphate(ATP)levels,which subsequently downregu-lates mcr-1 and conjugation-associated *** dual effects of chelerythrine can expand the use of antibiotic adjuvants and may provide a new strategy for circumventing mobile colistin resistance.

关键词： Chelerythrine Antibiotic adjuvant Conjugation inhibitor Colistin mcr-1 Dual effects

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P7C3-A20 treats traumatic brain injury in rats by inhibiting excessive autophagy and apoptosis

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Neural Regeneration Research 2024年第5期19卷 1078-1083页

作者： Zhiqing Yang Zhenchao Wang Xiaoqi Deng Lingxin Zhu Zhaomeng Song Changyu Cao Xinran Li School of Life Science and Engineering Foshan UniversityFoshanGuangdong ProvinceChina Foshan University veterinary Teaching Hospital FoshanGuangdong ProvinceChina

Traumatic brain injury is a severe health problem leading to autophagy and apoptosis in the brain.3,6-Dibromo-beta-fluoro-N-(3-methoxyphenyl)-9H-carbazole-9-propanamine(P7C3-A20)can be neuroprotective in various diseases,including ischemic stroke and neurodegenerative ***,whether P7C3-A20 has a therapeutic effect on traumatic brain injury and its possible molecular mechanisms are ***,in the present study,we investigated the therapeutic effects of P7C3-A20 on traumatic brain injury and explored the putative underlying molecular *** established a traumatic brain injury rat model using a modified weight drop method.P7C3-A20 or vehicle was injected intraperitoneally after traumatic brain *** neurological deficits were found in rats after traumatic brain injury,with deterioration in balance,walking function,and learning ***,hematoxylin and eosin staining showed significant neuronal cell damage,while terminal deoxynucleotidyl transferase mediated dUTP nick end labeling staining indicated a high rate of *** presence of autolysosomes was observed using transmission electron microscope.P7C3-A20 treatment reversed these pathological *** blotting showed that P7C3-A20 treatment reduced microtubule-associated protein 1 light chain 3-Ⅱ(LC3-Ⅱ)autophagy protein,apoptosis-related proteins(namely,Bcl-2/adenovirus E1B 19-kDa-interacting protein 3[BNIP3],and Bcl-2 associated x protein[Bax]),and elevated ubiquitin-binding protein p62(p62)autophagy protein ***,P7C3-A20 can treat traumatic brain injury in rats by inhibiting excessive autophagy and apoptosis.

关键词： apoptosis autophagy cortex hippocampus motor function P7C3-A20 traumatic brain injury

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Comment on “A novel antibiotic class targeting the lipopolysaccharide transporter”

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Pharmacology Discovery 2024年第2期4卷 52-53页

作者： Ruo-Yi Lv Xiao-Ye Liu Beijing Key laboratory of Traditional Chinese veterinary Medicine Beijing University of AgricultureBeijing 102206China Beijing Traditional Chinese veterinary Engineering Center Beijing University of AgricultureBeijing 102206China

Crisis of antibiotic resistant has become a global threat to public health in recent decades[1–4].Carbapenem antibiotics are a class of atypicalβ-lactam antibiotics with the broadest antibacterial spectrum and the strongest antibacterial ***,carbapenem-resistant Acinetobacter baumannii(CRAB)is stilly emerging,which is a gram-negative bacterium with lipopolysaccharide(LPS),which makes it resistant to several antibiotics and thus difficult to eliminate[5,6].As the number one pathogen of nosocomial infection,CRAB can cause severe pneumonia and bloodstream infection,*** mortality rate of patients infected with invasive CRAB can be as high as 40%to 60%,and it has been listed as the key pathogen of Class 1 by the World Health *** to the lack of feasible antibiotic strategies,phage combination therapy has been used in some patients,while the efficacy is limited[7].Therefore,new discovery and development of antibiotic that targeting CRAB is urgently ***,Kenneth A.

关键词： mortality listed eliminate

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