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Example Use of Low-Cost System for Capturing the Kinetic Parameters of Sperm Cells in Atlantic Salmon (<i>Salmo salar</i>)

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Advances in Bioscience and Biotechnology 2015年第2期6卷 63-72页

作者： Jorge Parodi Alfredo Ramírez-Reveco Guillermo Guerra laboratorio de Fisiología de la Reproducción Núcleo de Producción Alimentaria Escuela de Medicina Veterinaria Facultad de Recursos Naturales Universidad Católica de Temuco Temuco Chile laboratorio de criobiología y análisis de funcionalidad espermática Instituto de Ciencia Animal Facultad de Ciencias Veterinarias Universidad Austral de Chile Valdivia Chile

A spermiogramme is an analysis performed to assess the quality of semen. Motility parameters are primarily obtained by subjectively observing samples or by using complex systems, such as computer-assisted sperm analysis (CASA). Here, we describe an easy and low-cost analysis system for obtaining quantifiable kinetic observations using Salmo salar semen model. In this work, we observed and captured video images of both fresh and stored Atlantic salmon semen to describe the possibility of analysis using the ImageJ CASA plug-in application for the kinetic parameters obtained from the videos. The semen is exposed to “powermilt”, a commercial activating solution, and the curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP) values are described. When the samples were activated after having been stored, differences were detected in sperm quality, using the low-cost plug-in application. However, this system was not able to detect small variations in the same recorded sample, suggesting limits in sample observation. The results indicate that it is possible to quantify the kinetic parameters of semen samples using a low-cost video system and free software.

关键词： Fish Semen Motility Low Cost

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Inhibition of Acinetobacter baumannii Biofilm Formation by Methanolic Extract of Nothoscordum bivalve

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Advances in Microbiology 2018年第5期8卷 422-438页

作者： Hernández-Marín David Alejandro Munoz-Ortega Martín Humberto Guevara-Lara Fidel Gúzman-Lucio Marco Antonio Sánchez-García Eduardo laboratorio de Química Analítica Facultad de Ciencias BiológicasUniversidad Autónoma de Nuevo LeónSan Nicolás de los GarzaNuevo LeónMéxico laboratorio de Patología Molecular Experimental Departamento de QuímicaCentro de Ciencias BásicasUniversidad Autónoma de AguascalientesAguascalientesMéxico laboratorio de Biotecnología y funcionalidad de Alimentos Departamento de QuímicaCentro de Ciencias BásicasUniversidad Autónoma de AguascalientesAguascalientesMéxico departamento de Botánica Facultad de Ciencias BiológicasUniversidad Autónoma de Nuevo LeónMonterreyNuevo LeónMéxico

Acinetobacter baumannii is one of the most prevalent pathogens in nosocomial infections and has been cause of concern in recent years because, it has presented multiresistance to antibiotics and besides can form biofilms on biotic and abiotic surfaces like tissues and medical devices. Therefore, the search for new alternatives of natural origin to inhibit biofilm formation is being conducted. In the present investigation, sub-lethal concentrations (5.61 mg/mL, 3.74 mg/mL and 1.87 mg/mL) of methanolic extract of Nothoscordum bivalve were evaluated, showing biofilm formation inhibition up to 40.8%, in one nosocomial isolated of A. baumannii by the microtiter biofilm formation assay using crystal violet. On the other hand, the concentrations of 5.61 mg/mL and 3.74 mg/mL, caused an overexpression (up to 15.4 times) in the genes involved in the formation of biofilm (abaI, bap and csuE);due to this, the interaction of the extract with the bacteria was analyzed by scanning electron microscopy (SEM) and cellular damage was observed in the structure and stability of biofilm.

关键词： Biofilm Sub-Lethal Nothoscordum bivalve Acinetobacter baumannii

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