Example Use of Low-Cost System for Capturing the Kinetic Parameters of Sperm Cells in Atlantic Salmon (<i>Salmo salar</i>)
Example Use of Low-Cost System for Capturing the Kinetic Parameters of Sperm Cells in Atlantic Salmon (<i>Salmo salar</i>)作者机构：Laboratorio de Fisiología de la Reproducción Núcleo de Producción Alimentaria Escuela de Medicina Veterinaria Facultad de Recursos Naturales Universidad Católica de Temuco Temuco Chile Laboratorio de Criobiología y Análisis de Funcionalidad Espermática Instituto de Ciencia Animal Facultad de Ciencias Veterinarias Universidad Austral de Chile Valdivia Chile
出 版 物：《Advances in Bioscience and Biotechnology》 (生命科学与技术进展（英文）)
年 卷 期：2015年第6卷第2期
页 面：63-72页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Fish Semen Motility Low Cost
摘 要：A spermiogramme is an analysis performed to assess the quality of semen. Motility parameters are primarily obtained by subjectively observing samples or by using complex systems, such as computer-assisted sperm analysis (CASA). Here, we describe an easy and low-cost analysis system for obtaining quantifiable kinetic observations using Salmo salar semen model. In this work, we observed and captured video images of both fresh and stored Atlantic salmon semen to describe the possibility of analysis using the ImageJ CASA plug-in application for the kinetic parameters obtained from the videos. The semen is exposed to “powermilt, a commercial activating solution, and the curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP) values are described. When the samples were activated after having been stored, differences were detected in sperm quality, using the low-cost plug-in application. However, this system was not able to detect small variations in the same recorded sample, suggesting limits in sample observation. The results indicate that it is possible to quantify the kinetic parameters of semen samples using a low-cost video system and free software.
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