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American Journal of Analytical Chemistry 2014年第4期5卷 249-257页

作者： Veniero Gambaro Fiorenza Farè Andrea Barlocco Federico Maggi Giuseppe Simoni Lucia Dell’Acqua Chiara Rusconi Gabriella Roda Dipartimento di Scienze Farmaceutiche “Pietro Pratesi” University of Milan Milan Italy laboratorio toma srl Busto Arsizio Italy

The Smith-Lemli-Opitz syndrome (SLOS), is an autosomal recessive disorder caused by a 7-dehydrocholesterol reductase deficiency, which is characterized by abnormally elevated amniotic fluid 7-DHC (7-dehydrocholesterol) concentrations. A GC/FID (gas-chromatography with a flame ionization detector) and GC/MS (gas-chromatography with a mass detector) method was optimized for the detection of cholesterol and 7-DHC in amniotic fluids. The quantitative determination of cholesterol in 39 control amniotic fluids evidenced that between the fourth and fifth month of pregnancy the levels of this analyte are quite constant, the concentration of total and free cholesterol being respectively 10.3 μg·mL﹣1 (SD = ±3.6) and 1.7 μg·mL﹣1 (SD = ±0.91), while the analysis of 60 amniotic fluids potentially related to SLOS, showed a higher variability of cholesterol levels. Moreover, in 13 samples an analyte which did not correspond either to cholesterol or to 7-DHC was detected. A GC/MS investigation allowed us to identify this compound as lathosterol, a precursor of cholesterol in the biosynthetic pathway.

关键词： Lathosterol Cholesterol SLO Syndrome Amniotic Fluid GC/FID GC/MS

<i>Opuntia ficus-indica</i>Pruning Waste Recycling: Recovery and Characterization of Mucilage from Cladodes

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Natural Resources 2021年第4期12卷 91-107页

作者： Silvia Procacci Emanuel Bojórquez-Quintal Giovambattista Platamone Oliviero Maccioni Vania Lo Vecchio Vincenzo Morreale Chiara Alisi Roberto Balducchi Loretta Bacchetta ENEA Casaccia Division Biotecnology and Agroindustry Rome Italy CONACYT-laboratorio de Análisis y Diagnóstico del Patrimonio El Colegio de Michoacán La Piedad Mexico Bioinagro srl Agrofoods and Biochemicals Licata Italy ENEA Casaccia Division Protection and Enhancement of the Natural Capital Rome Italy

The present work focuses on the proximate physical and chemical profile of Opuntia ficus-indica mucilage, mechanically extracted from cladodes, a waste of pruning in traditional organic cactus pear orchards in Italy (San Cono, Sicily). The mechanical extraction increased the mucilage yield to 30% dry weight. Physical characterization concerns pH, viscosity, free acidity and density, useful for emulsifying capacity. Spectrophotometric analysis was applied to assess total carbohydrates, proteins, uronic acids, total polyphenols content and antioxidant capacity. DART-MS and SEM-EDX were performed to evaluate functional mucilage components and relative amounts of minerals, respectively. From the main results Opuntia ficus-indica by-product, in addition to the preponderant total carbohydrates content, shows the high concentration in calcium and potassium and a fair amount of health-promoting phytochemicals, which make it a good candidate for the different type of industrial applications.

关键词： Natural Hydrocolloid Opuntia ficus-indica (L) Mill. DART-MS Analysis Chemical Analysis Mucilage Extraction Cladodes

An inter-laboratory study of DNA-based identity,parentage and species testing in animal forensic genetics

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Forensic Sciences Research 2022年第4期7卷 708-713页

作者： Sreetharan Kanthaswamy Torsten Brendel Luis Cancela Denise A.Andrade de Oliveira Bertram Brenig Carmen Cons Julian A.Crespi Markéta Dajbychová Andreas Feldl Tomohito Itoh Vincenzo Landi Amparo Martinez Malgorzata Natonek-Wisniewska Robert F.Oldt Anna Radko Oscar Ramírez Clementina Rodellar Manuel Ruiz-Girón David Schikorski María Elena Turba Guillermo Giovambatista School of Mathematics and Natural Sciences Arizona State University(ASU)at the West CampusGlendaleAZUSA California National Primate Research Center University of CaliforniaDavisCAUSA Evolutionary Biology Graduate Program School of Life SciencesArizona State UniversityTempeAZUSA Eurofins Genomics Europe Applied Genomics GmbH EbersbergGermany IDENTITAS MontevideoUruguay Laboratório de Genética Escola de Veterinária da UFMGBelo HorizonteBrazil Institute of Veterinary Medicine University of GoettingenGoettingenGermany Facultad de Veterinaria Laboratorio de Genética Bioquímica(LAGENBIO)Instituto Agroalimentario de Aragón-IA2(Universidad de Zaragoza-CITA)ZaragozaSpain Facultad de Ciencias Veterinarias UNLP IGEVET-Instituto de Genética Veterinaria(UNLP-CONICET LAPLATA)La PlataArgentina Genomia s.r.o. PlzeňCzech Republic Maebashi Institute of Animal Science Livestock Improvement Association of Japan(LIAJ)MaebashiJapan Department of Veterinary Medicine Animal Breeding and GeneticsUniversity of Bari“Aldo Moro”ValenzanoItaly Department of Genetics Faculty of Veterinary SciencesUniversity of CórdobaCórdobaSpain Department of Animal Molecular Biology National Research Institute of Animal ProductionBalicePoland Vetgenomics SL BarcelonaSpain laboratorio de Biología Molecular y Genómica Hispalis Biolab S.L.U.SevilleSpain Laboratoire LABOFARM-GENINDEXE LOUDEACCedexFrance Genefast srl ForlìItaly

The probative value of animal forensic genetic evidence relies on laboratory accuracy and ***-laboratory comparisons allow laboratories to evaluate their performance on specific tests and analyses and to continue to monitor their *** International Society for Animal Genetics(ISAG)administered animal forensic comparison tests(AFCTs)in 2016 and 2018 to assess the limitations and capabilities of laboratories offering forensic identification,parentage and species determination *** AFCTs revealed that analyses of low DNA template concentrations(≤300 pg/μL)constitute a significant challenge that has prevented many laboratories from reporting correct identification and parentage ***,a lack of familiarity with species testing protocols,interpretation guidelines and representative databases prevented over a quarter of the participating laboratories from submitting correct species determination *** laboratories showed improvement in their genotyping accuracy over ***,the use of forensically validated standards,such as a standard forensic short tandem repeat(STR)kit,preferably with an allelic ladder,and stricter guidelines for STR typing,may have prevented some common issues from occurring,such as genotyping inaccuracies,missing data,elevated stutter products and loading *** AFCTs underscore the importance of conducting routine forensic comparison tests to allow laboratories to compare results from each *** should keep improving their scientific and technical capabilities and continuously evaluate their personnel’s proficiency in critical techniques such as low copy number(LCN)analysis and species *** this is the first time that the ISAG has conducted comparison tests for forensic testing,findings from these AFCTs may serve as the foundation for continuous improvements of the overall quality of animal forensic genetic testing.

关键词： Forensic sciences forensic genetics comparison test forensic DNA analysis individual identification parentage test species test

Efficient fully blade-coated perovskite solar cells in air with nanometer-thick bathocuproine buffer layer

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Nano Research 2021年第4期14卷 1034-1042页

作者： Sergio Castro-Hermosa Luana Wouk Izabela Silva Bicalho Luiza de Queiroz Correa Bas de Jong Lucio Cina Thomas M.Brown Diego Bagnis CSEM Brasil Avenida Jose Candido da Silveira200031035-536 Belo HorizonteBrazil CHOSE(Centre for Hybrid and Organic Solar Energy) Department of Electronic EngineeringUniversity of Rome Tor VergataVia del Politecnico 100133 RomeItaly Hydro Engineering and Agricultural Development Research Group(GHIDA) Faculty of EngineeringUniversidad SurcolombianaAvenida Pastrana Borrero-Carrera 1410001 NeivaColombia Cicci Research srl via Giordania 227 58100 GrossetoItaly Department of Biotechnology Chemistry and PharmacyUniversity of SienaVia A.Moro 253100 SienaItaly

Fully printed perovskite solar cells(PSCs)were fabricated in air with all constituent layers,except for electrodes,deposited by the blade coating *** PSCs incorporated,for the first time,a nanometer-thick printed bathocuproine(BCP)hole blocking buffer using blade coating and deposited at relative humidity up to 50%.The PSCs with a p-i-n structure(glass/indium tin oxide(ITO)/poly(3,4-ethylenedioxythiophene)polystyrene sulfonate(PEDOT:PSS)/CH_(3)NH_(3)Pbl_(3)/[6,6]-phenyl-C_(61)-butyric acid methyl ester(PCBM)/BCP/Ag)delivered a maximum power conversion efficiency(PCE)of 14.9%on an active area of 0.5 cm^(2)when measured under standard test *** PSCs with a blade coated BCP delivered performance of 10%and 63%higher(in relative terms)than those incorporating a spin coated BCP or without any BCP film,*** atomic force microscopy(AFM)showed that blade coated films were more homogeneous and acted also as a surface planarizer leading to a reduction of roughness which improved BCP/Ag interface lowering charge *** demonstration of 15%efficient devices with all constituent layers,including nanometer-thick BCP(〜10 nm),deposited by blade coating in air,demonstrates a route for industrialization of this technology.

关键词： perovskite buffer bathocuproine(BCP) blade coating printed electronics

Profiling of phenol content and microbial community dynamics during pate olive cake fermentation

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Food Bioscience 2023年第2期52卷 170-178页

作者： Paola Foti Nunziatina Russo Cinzia LRandazzo Altino Branco Choupina Alessandra Pino Cinzia Caggia Flora V.Romeo Dipartimento di Agricoltura Alimentazione e Ambiente-Di3AUniversita degli Studi di Cataniavia S.Sofia 10095123CataniaItaly ProBioEtna srl Spin off of University of Cataniavia S.Sofia 10095123CataniaItaly Centro de Investigacao de Montanha(CIMO) Instituto Politecnico de BragancaCampus de Santa Apolonia5300-253BraganccPortugal laboratorio para a Sustentabilidade e Tecnologia em Regioes de Montanha Instituto Politecnico de BragancaCampus de Santa Apolonia5300-253BragançaPortugal Consiglio per la Ricerca in Agricoltura e l’Analisi dell’Economia Agraria(CREA) Centro di Ricerca OlivicolturaFrutticoltura e AgrumicolturaCorso Savoia 19095024AcirealeItaly

In this study,different microbial strains,as single and mixed-cultures,were used to ferment the pate olive cake(POC),a by-product of olive oil *** particular,strains belonging to Candida boidinii,Wickerhamomyces anomalus and Lactiplantibacillus plantarum were *** fermentation was carried out on diluted(3:2)POC without and with glucose(2%w/v)***,phenolic compounds were monitored during fermentation in POC added with glucose differetly inoculated and the microbial community,at the end of fermentation,was evaluated by Next Generation Sequencing(NGS)*** highlighted that inoculated samples showed an hydroxytyrosol content higher than the un-inoculated *** particular,during fermentation the sample inoculated with ***,both in single and in mixed culture together with ***,increased the hydroxytyrosol content by 275 and 261 mg/L,respectively,after 8 days,to reach the highest content at the end of *** analysis revealed a low abundance of 16S ribosomal RNA genes and fungal ITS in all samples at any sampling ***,at the end of fermentation,all samples exhibited a different bacterial community with a decrease in acetic acid bacteria and an increase in Lactobacillaceae ***,no effect was detected in any samples on fungal metagenomic profile,where Dipodascus geotrichum was found dominant both at initial and final *** conclusion,the present study confirmed that selected cultures can drive the fermentation and have an impact on the phenolic profile.

关键词： Olive by-products Yeast Lactic acid bacteria Fermentation Phenols Microbial dynamics