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Antioxidant capacity and genoprotective effect of ethanol fruit extract from detarium microcarpum Guill. and Perr.(Caesalpiniaceae)

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Asian Pacific Journal of Tropical Biomedicine 2017年第1期7卷 32-36页

作者： AblasséRouamba Maurice Ouedraogo Martin Kiendrebeogo laboratoire de biochimie et Chimie appliqu′ees(LABIOCA) UFR-SVTUniversit′e de Ouagadougou03 BP 7021Ouagadougou 03Burkina Faso laboratoire de Physiologie Animale(LAPA) UFR-SVTUniversit′e de Ouagadougou09 BP 848Ouagadougou 09Burkina Faso

Objective: To evidence the ability of ethanol fruit extract from detarium microcarpum(D. microcarpum) to preserve DNA integrity against oxidative genomic ***: ethanol extract from D. microcarpum fruit pulp was analyzed for its antioxidant capacity using ferric reducing antioxidant power, 2,2-diphenyl-1-picrylhydrazyl,2,20-azinobis-3-ethyl-ethylbenzothiazoline-6-sulphonate, superoxide anion, deoxyribose degradation and lipid peroxidation models. The genoprotective activity was assessed ex vivo by comet assay, on liver cells of NMRI female mice using cyclophosphamide(CP) as genotoxic ***: ethanol extract from D. microcarpum fruit pulp exhibited interesting antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl, deoxyribose degradation and lipid peroxidation assays. The extract did not present any genotoxic effect but protected DNA against CP-induced damages with a dose-dependent manner. The genoprotective effect observed was related to the antioxidant molecules of the fruit that scavenged the hydroxyl radical(generated by the metabolism of CP) as well as the peroxyl and alkoxyl radicals issued from lipid peroxidation. Other mechanisms such as inactivation of CP metabolism to genotoxic end products, induction of the expression of antioxidant and DNA repair enzymes have been ***: Our results suggest that the wild edible fruit from D. microcarpum could be beneficial on consumer's health by its antioxidant and genoprotective effects, particularly during chemotherapies exhibiting genotoxic effects like CP in cancer treatment.

关键词： Antioxidant activity Comet assay detarium microcarpum Genoprotection

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Cytotoxic, antioxidant and antimicrobial activities of Nerium oleander collected in Morocco

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Asian Pacific Journal of Tropical Medicine 2019年第1期12卷 32-37页

作者： Messaoudi Mouhcine Laglaoui Amin Amzazi Saaid Hammani Khalil Benbacer Laila el Mzibri Mohammed Unité de Biologie et Recherche Médicale CNESTEN laboratoire de biochimie-immunologie Faculté des Sciences Mohammed V University équipe de Recherche en Biotechnologies & Génie des Biomolécules Faculté des Sciences et Techniques de Tanger Abdelmalek Essaadi University laboratoire de Ressources Naturelles et environnement Faculté Polydisciplinaire de Taza Sidi Mohammed Ben Abdellah University

Objective: To investigate the cytotoxic, antioxidant and antibacterial activities of ethanolic and aqueous crude extracts of Nerium oleander(N. oleander) leaves. Methods: Cytotoxic activities were evaluated by WST-1 bioassay on two human cancer cell lines, namely human colon adenocarcinoma cell line HT-29 and human breast cancer cell line MDA-MB-231. The antioxidant property of N. oleander extracts was assessed by DPPH scavenging and he agar disc diffusion method was used for the determination of an毬-carotene bleaching tests. Ttimicrobial activity against different strains. Results: Using cell viability indices, the WST-1 test revealed that both extracts reduced cell viability in both cell lines. Our results also showed that aqueous extract was more active than ethanolic extract, with IC50 values of(1.67±0.22) μg/mL and(2.36±0.44) μg/mL on MDA-MB-231 cells, and(2.89±0.35) μg/mL and(5.09±0.52) μg/mL on HT29 cells, respectively. The study of the antioxidant activity showed that N. oleander extracts had a considerable scavenging capacity and exerted a significant preventive effect against the oxidation of 毬-carotene by the peroxide radicals. However, the antibacterial test showed that both ethanolic and aqueous extracts of N. oleander had a moderate antibacterial effect limited only to Gram-positive bacteria. Conclusions: Our results shows that N. oleander aqueous and ethanolic extracts have significant cytotoxic activities against tumor cell lines and possesses a strong antioxidant capacity, suggesting the presence of active compounds in N. oleander leaves that could be a potential source of phytochemicals with high medicinal value to be used in cancer treatment and prevention.

关键词： Nerium oleander Anti-cancer Cytotoxicity Antioxidant Antimicrobial Bioactive compounds

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Methodology of factorial design deriving guidelines for simulation of growth curve and production of sugars by Spirulina (Arthrospira) maxima

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Journal of Central South University of Technology 2001年第4期8卷 228-233页

作者： NIe Zhen yuan XIA Jin lan LeVeRT J.M. Institute of Bioengineering Department of Mineral EngineeringCentral South UniversityChangsha 410083China Service de Chimie et biochimie appliquées FacultéPolytechnique de MonsMons 7000Belgique

It is practical, economic and sometimes essential to derive rules or conclusions by performing lesser runs of experiments. In this part, a methodology based on 2 f factorial design was brought up to derive guidelines to simulate growth curve and production of sugars by Spirulina (Arthrospira) maxima . The growth curve or accumulation process of sugars was idealized by sets of straight lines limited by phase transfers of growth or accumulation of sugars. Normal analyses of the critical values of the transfers were used to derive their linear relationships with the initial conditions of the experimental factors. These linear functions were called guidelines and were used to simulate the growth curve or accumulation of sugars. Generalization of the guideline technique was determined by the kinetic limitation of nutrient nitrogen or sulfur that was dependent upon their stoichiometric deficiency directly derived from their initial values in the medium. This method uses the initial conditions of culture and does not need measurements of concentrations of nitrate, sulfate and pigments during cultivation. It is a practical and useful alternative way to trace and predict approximately the growth curve and production of sugars by S. maxima .

关键词： factorial design guideline simulation growth production of sugar Arthrospira Spirulina maxima

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(e) enol ethers from the stereoselective reduction of α-alkoxy-β-ketophosphonates and Wittig type reaction

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Science China Chemistry 2010年第9期53卷 1937-1945页

作者： DAYOUB Wissam DOUTHeAU Alain laboratoire de Catalyse et Synthèse Organique Institut de Chimie et Biochimie Moléculaires et Supramoléculaires(ICBMS)CNRSUMR5246Université Lyon 1 Institut National des Sciences appliquées de Lyon Laboratoire de Chimie Organique et BioorganiqueICBMS UMR 5246 CNRS Université Lyon 1 INSA Lyon

When α-alkoxy-β-ketophosphonates,prepared by the Rh(Ⅱ) mediated insertion reaction of α-diazo-β-ketophosphonates into the OH bond of primary alcohols,were reduced either by NaBH4 in the presence of CaCl2 or by DIBAL,they respectively gave the corresponding anti or syn stereomeric hydroxyphosphonates with pronounced to complete *** to the action of potassium tert-butoxyde,syn isomers led to the corresponding pure(e) enol ethers in moderate to good *** the same conditions anti isomers led to a mixture of(Z) and(e) enol ethers in rather poor *** sequence was applied to the preparation of some allyl-vinyl ethers with a(e) configuration for the vinylic double bond.

关键词： α-alkoxy-β-ketophosphonates reduction insertion rhodiocarbenoids Wittig-Wadsworth-emmons enol ethers

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Fractionation and characterization of polysaccharides from cyanobacterium Spirulina (Arthrospira) maxima in nitrogen-limited batch culture

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Journal of Central South University of Technology 2002年第2期9卷 81-86页

作者：聂珍媛夏金兰 J.M.LeVeRT Institute of Bioengineering Faculty of Resource Processing and Bioengineering Central South University Changsha 410083 China Service de Chimie et biochimie appliquées Faculté Polytechnique de Mons Mons 7000 Belgque

By a sequentially selective extraction procedure, polysaccharides (PS) from cyanobacterium Spirulina (Arthrospira) maxima that can be bio functionally healthy components were fractionated in four parts including the culture medium (PSCM), the external layers of the cell (PSeL), the cell wall (PSCW) and storage granules (PSSG). The four fractionated parts of polysaccharides were characterized by gas liquid chromatography (GLC) equipped with FID and fused silica capillary column (15 m×0.53 mm i. d.). The contents of PSCM, PSeL, PSCW and PSSG were about 2.0%, 2.6%, 10.0% and 52.0% to cell dry matter, respectively. Glucose was almost the only monosaccharide in PSSG and PSCW and most predominant in PSeL, while in PSCM, xylose, rhamnose and glucose were the main ones. Two uronic acids represented by glucuronic acid and galacturoic acid, six neutral monosaccharides including fucose, rhamnose, xylose, mannose, galactose and glucose, and two possible unidentified sugars were found in PSeL and PSCM. These data are valuable for the selective productions of high added value sugars from Spirulina .

关键词： characterization cyanobacterium extraction fractionation gas liquid chromatography polysaccharides Spirulina (Arthrospira) maxima

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Fermentation effect on the Nutrient and Antinutrient Composition of <i>Senegalia macrostachya</i>and <i>Parkia biglobosa</i>Seeds: A Comparative Study

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Food and Nutrition Sciences 2020年第7期11卷 726-740页

作者： Aimée W. D. B. Guissou Charles Parkouda Coulibaly K. Anaïs Traoré Korotimi edwige Bahanla Oboulbiga Aly Savadogo Département Technologie Alimentaire (DTA) Institut de Recherche en Sciences Appliquées et Technologies (IRSAT) Centre National de la Recherche Scientifique et Technologique (CNRST) Ouagadougou Burkina Faso laboratoire de biochimie et d’immunologie appliquée Département de Biochimie-Microbiologie Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

This study aimed to evaluate the effect of the fermentation on the nutrient and antinutrient composition of Senegalia macrostachya and Parkia biglobosa seeds. For condiments production, the raw seeds were cleaned, cooked, drained, washed, cooked, drained and fermented. Results show that the pH increased (from 6.09 to 7.76 and from 6.99 to 7.92) from the onset of the fermentation till 48 h during fermentation of <

关键词： Senegalia macrostachya Parkia biglobosa Fermentation Nutrients Phytates

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Induction of antiproliferative effect by diosgenin through activation of p53, release of apoptosis-inducing factor (AIF) and modulation of caspase-3 activity in different human cancer cells

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Cell Research 2004年第3期14卷 188-196页

作者： Cecile CORBIeRe,Bertrand LIAGRe,Faraj TeRRO,Jean-Louis BeNeYTOUT laboratoire de biochimie UPReS eA 1085 Faculté de Pharmacie rue du Docteur Marcland 87025 Limoges Cedex France laboratoire de biochimie UPReS eA 1085 Faculté de Pharmacie rue du Docteur Marcland 87025 Limoges Cedex France Service d'Histologie et Biologic Cellulaire Faculte de Medecine rue du Docteur Marcland 87025 Limoges Cedex France laboratoire de biochimie UPReS eA 1085 Faculté de Pharmacie rue du Docteur Marcland 87025 Limoges Cedex France

Previously, we demonstrated that a plant steroid, diosgenin, altered cell cycle distribution and induced apoptosis in the human osteosarcoma 1547 cell line. The objective of this study was to investigate if the antiproliferative effect of diosgenin was similar for different human cancer cell lines such as laryngocarcinoma Hep-2 and melanoma M4Beu cells. Moreover, this work essentially focused on the mitochondrial pathway. We found that diosgenin had an important and similar antiproliferative effect on different types of cancer cells. In addition, our new results show that diosgenin-induced apoptosis is caspase-3 dependent with a fall of mitochondrial membrane potential, nuclear localization of AIF and poly (ADP-ribose) polymerase cleavage. Diosgenin treatment also induces p53 activation and cell cycle arrest in the different cell lines studied.

关键词： diosgenin apoptosis p53 AIF caspase cancer cells.

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One-step selective synthesis of branched 1-O-alkyl-glycerol/diglycerol monoethers by catalytic reductive alkylation of ketones

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Science China Chemistry 2010年第9期53卷 1953-1956页

作者： DAYOUB Wissam LeMAIRe Marc laboratoire de Catalyse Synthèse et environnement Institut de Chimie et Biochimie Moléculaires et Supramoléculaires(ICBMS)CNRSUMR5246Université Lyon 1

Branched 1-O-alkyl glycerol and diglycerol monoethers were obtained in good yields and high selectivity by a straightforward catalytic reductive alkylation of glycerol with relevant ketones in the presence of 0.5 mol%... 详细信息

关键词： glycerol monoether diglycerol monoether 1-O-alkylation Pd/C reduction

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Helicobacter pylori arginase mutant colonizes arginase Ⅱ knockout mice

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World Journal of Gastroenterology 2011年第28期17卷 3300-3309页

作者： Songhee H Kim Melanie L Langford Jean-Luc Boucher Traci L Testerman David J McGee department of Microbiology and Immunology Louisiana State University Health Sciences Center-Shreveport University of Nebraska-Lincoln School of Biological SciencesLincoln laboratoire de Chimie et biochimie Pharmacologiques et Toxicologiques Université Paris Descartes

AIM: To investigate the role of host and bacterial arginases in the colonization of mice by Helicobacter pylori (***).MetHODS: *** produces a very powerful urease that hydrolyzes urea to carbon dioxide and ammonium,which neutralizes *** is absolutely essential to *** pathogenesis;therefore,the urea substrate must be in ample supply for urease to work *** urea substrate is most likely provided by arginase activity,which hydrolyzes L-arginine to L-ornithine and *** work has demonstrated that *** arginase is surprisingly not required for colonization of wild-type ***,another in vivo source of the critical urea substrate must *** hypothesized that the urea source was provided by host arginase Ⅱ,since this enzyme is expressed in the stomach,and *** has previously been shown to induce the expression of murine gastric arginase Ⅱ.To test this hypothesis,wild-type and arginase (rocF) mutant *** strain SS1 were inoculated into arginase Ⅱ knockout ***: Surprisingly,both the wild-type and rocF mutant bacteria still colonized arginase Ⅱ knockout ***,feeding arginase Ⅱ knockout mice the host arginase inhibitor S-(2-boronoethyl)L-cysteine (BeC),while inhibiting > 50% of the host arginase Ⅰ?activity in several tissues,did not block the ability of the rocF mutant *** to *** contrast,BeC poorly inhibited *** arginase ***: The in vivo source for the essential urea utilized by *** urease is neither bacterial arginase nor host arginase Ⅱ;instead,either residual host arginase Ⅰ?or agmatinase is probably responsible.

关键词： Arginase Helicobacter pylori S-(2-boronoethyl)-L-cysteine Urease Mice

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Frequent mutations of the CA simple sequence repeat in intron 1 of eGFR in mismatch repair-deficient colorectal cancers

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World Journal of Gastroenterology 2008年第7期14卷 1053-1059页

作者： Marie-Pierre Buisine Agnès Wacrenier Christophe Mariette emmanuelle Leteurtre Fabienne escande Sana Aissi Amandine Ketele Annette Leclercq Nicole Porchet Thécla Lesuffleur Centre de RechercheJean-Pierre Aubert-JPARC INSERM U837Equipe Mucinesdifférenciation et cancérogenèseépithélialesLaboratoire deBiochimie et Biologie MoléculaireCHRU de Lilleand Facultéde Médecine H.WarembourgUniversitéde Lille 2LilleFrance laboratoire d'Anatomie et CytologiePathologiques CHRU de LilleLilleFrance Centre de Recherche Jean-Pierre Aubert-JPARC INSERM U837Service de Chirurgie Digestiveet GénéraleCHRU de Lilleand Facultéde Médecine H.WarembourgUniversitéde Lille 2LilleFrance Centre de Recherche Jean-Pierre Aubert-JPARC INSERM U837Laboratoire d'Anatomie et Cytologie PathologiquesCHRU de Lilleand Facultéde Médecine H.WarembourgUniversitéde Lille 2LilleFrance Centre de Recherche Jean-Pierre Aubert-JPARC INSERM U837and Laboratoire de Biochimie etBiologie MoléculaireCHRU de LilleLilleFrance Centre de Recherche Jean-Pierre Aubert-JPARC INSERM U837Laboratoire de Biochimie et Biologie MoléculaireCHRU de LilleLilleFrance Laboratoire d'Immunologie et GénétiqueTunis-ElManarTunisie Centre de Recherche Jean-Pierre Aubert-JPARC INSERM U837LilleFrance laboratoire de biochimie et Biologie Moléculaire CHRU de LilleLilleFrance Centre de RechercheJean-Pierre Aubert-JPARC INSERM U837Equipe Mucinesdifférenciation et cancérogenèseépithélialesLaboratoire de Biochimie et Biologie MoléculaireCHRU de Lilleand Facultéde Médecine H.WarembourgUniversitéde Lille 2LilleFrance present address INSeRMU843 Université Paris 7Hpital R.DebréParisFrance

AIM:To investigate the polymorphic simple sequencerepeat in intron 1 of the epidermal growth factor receptorgene(eGFR)(CA-SSRⅠ),which is known to affect theefficiency of gene transcription as a putative target of themismatch repair(MMR)machinery in colorectal ***:The CA-SSRⅠgenotype was analyzed ina total of 86 primary colorectal tumors,selected upontheir microsatellite instability(MSI)status[42 with highfrequency MSI(MSI-H)and 44 microsatellite stable(MSS)]and their respective normal *** effect of the CA-SSRⅠgenotype on the expression of the eGFR gene wasevaluated in 18 specimens using quantitative real-timereverse transcription PCR and ***:Mutations in CA-SSRⅠwere detected in 86%(36 of 42)of MSI-H colorectal tumors and 0%(0 of 44)ofMSS tumors,indicating the eGFR gene as a novel putativespecific target of the defective MMR system(Ped expression of eGFR was detected in most ofthe colorectal tumors analyzed[6/12(50%)at the mRNAlevel and 15/18(83%)at the peptide level].However,noassociation was apparent between eGFR expression andCA-SSRⅠstatus in tumors or normal ***:Our results suggest that CA-SSRⅠsequence does not contribute to the regulation of eGFRtranscription in colon,and should thus not be consideredas a promising predictive marker for response to eGFRinhibitors in patients with colorectal cancer.

关键词： epidermal growth factor receptor Microsatellite instability Allelic imbalance Gene polymorphism expression Colorectal cancer

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