Helicobacter pylori arginase mutant colonizes arginase Ⅱ knockout mice

作     者：Songhee H Kim Melanie L Langford Jean-Luc Boucher Traci L Testerman David J McGee 

作者机构：Department of Microbiology and ImmunologyLouisiana State University Health Sciences Center-Shreveport University of Nebraska-LincolnSchool of Biological SciencesLincoln Laboratoire de Chimie et Biochimie Pharmacologiques et ToxicologiquesUniversité Paris Descartes 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2011年第17卷第28期

页      面：3300-3309页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by Public Health Service grant R01-CA101931 (to DJM) from the National Institutes of Health 

主　　题：Arginase Helicobacter pylori S-(2-boronoethyl)-L-cysteine Urease Mice 

摘      要：AIM: To investigate the role of host and bacterial arginases in the colonization of mice by Helicobacter pylori (***).METHODS: *** produces a very powerful urease that hydrolyzes urea to carbon dioxide and ammonium,which neutralizes *** is absolutely essential to *** pathogenesis;therefore,the urea substrate must be in ample supply for urease to work *** urea substrate is most likely provided by arginase activity,which hydrolyzes L-arginine to L-ornithine and *** work has demonstrated that *** arginase is surprisingly not required for colonization of wild-type ***,another in vivo source of the critical urea substrate must *** hypothesized that the urea source was provided by host arginase Ⅱ,since this enzyme is expressed in the stomach,and *** has previously been shown to induce the expression of murine gastric arginase Ⅱ.To test this hypothesis,wild-type and arginase (rocF) mutant *** strain SS1 were inoculated into arginase Ⅱ knockout ***: Surprisingly,both the wild-type and rocF mutant bacteria still colonized arginase Ⅱ knockout ***,feeding arginase Ⅱ knockout mice the host arginase inhibitor S-(2-boronoethyl)L-cysteine (BEC),while inhibiting 50% of the host arginase Ⅰ?activity in several tissues,did not block the ability of the rocF mutant *** to *** contrast,BEC poorly inhibited *** arginase ***: The in vivo source for the essential urea utilized by *** urease is neither bacterial arginase nor host arginase Ⅱ;instead,either residual host arginase Ⅰ?or agmatinase is probably responsible.