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Journal of Environmental Sciences 2017年第1期29卷 44-51页

作者： Wafa Kharroubi Samia Hai Ahmed Thomas Nury Pierre Andreoletti Rachid Sakly Mohamed Hammami Gerard Lizard Univ.Bourgogne Franche-Comte Laboratory bio-peroxil Biochemistry of the PeroxisomeInflammation and Lipid Metabolism EA7270/INSERMFaculty of Sciences GabrielDijon 21000France Laboratory of Nutrition-Functional Foods and Vascular Diseases Faculty of MedicineUniversity of MonastirMonastir 5019Tunisia

The treatment of microglial BV-2 cells with sodium arsenate（As（V）：0.1-400 μmol/L — 48 hr）induces a dose-dependent *** neurotoxic effects of high concentrations of As（V）（100,200 and 400 μmol/L） are characterized by increased levels of mitochondrial complexesⅠ,Ⅱ,and Ⅳ followed by increased superoxide anion ***,As（V） triggers an apoptotic mode of cell death,demonstrated by an apoptotic SubG1 peak,associated with an alteration of plasma membrane *** is also a decrease in transmembrane mitochondrial potential and mitochondrial adenosine triphosphate *** is therefore tempting to speculate that As（V） triggers mitochondrial dysfunction,which may lead to defective oxidative phosphorylation subsequently causing mitochondrial oxidative damage,which in turn induces an apoptotic mode of cell death.

关键词： Sodium arsenate Microglial BV-2 cells Mitochondrial dysfunction Oxidative phosphorylation complexes Superoxide anions Apoptosis

Differential Alterations of Lipid Status and Lipid Metabolism, Induction of Oxidative Stress by Sodium Arsenate in Female Rat's Liver and Kidney

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biomedical and Environmental Sciences 2015年第11期28卷 829-833页

作者： Kharroubi Wafa Dhibi Madiha Chreif Imed Gérard Lizard Hammami Mohamed Sakly Rachid Laboratory of Nutrition-Functional Foods and Vascular Diseases Faculty of Medicine University of Monastir laboratoire bio-peroxil ‘Biochimie du Peroxysome Inflammation et Métabolisme Lipidique’ EA7270/INSERMFaculté des Sciences Gabriel

Three groups of female rats （n=6 per group） were exposed to 1 and 10 mg/L of sodium arsenate for 90 days. The antioxidant and peroxidation parameters in kidney and liver tissues were evaluated, also the fatty acid composition were determined. In liver the synthesis pathway of n-3 polyunsaturated fatty acids （PUFA） seemed to be more affected than the n-6 PUFA, while in kidney the n-6 pathway was more affected.

关键词： PUFA Differential Alterations of Lipid Status and Lipid Metabolism

Effects of Cadmium on Polar Lipid Composition and Unsaturation in Maize(Zea mays) in Hydroponic Culture

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Journal of Integrative Plant biology 2007年第12期49卷 1693-1702页

作者： Radhouane Chaffai Tinni Nouhou Seybou Brahim Marzouk Ezzedine EI Ferjani laboratoire de bio-Physiologie Cellulaires Vegetales Faculte des Sciences de Bizerte 7021 Zarzouna-BizerteTunisie laboratoire ERTAC Physiologie et Genetique Vegetales 63177 AubiereClermont FerrandFrance laboratoire d'Adaptation et d'Amelioration des Plantes Institut National de la Recherche Scientifique et Technique(INRST)BP 95-2050 Hammam-LifTunisie

This study aimed to evaluate the effect of Cd exposure （100 μmol/L） on polar lipid composition, and to examine the level of fatty acid unsaturation in maize （Zea mays L.）. In roots, the level of 16：0 and monounsaturated fatty acids （16：1 ＋ 18：1） decreased in phosphatidylcholine （PC） and phosphatidylethanolamine （PE）. In contrast, the proportion of unsaturated 18-C fatty acid species showed an opposite response to Cd. The content, on the other hand, of PC, PE, digalactosyldiacylglycerol （DGDG）, and steryl lipids increased in roots （2.9-, 1.6-, 5.3-, and 1.7-fold increase, respectively）. These results suggest that a more unsaturated fatty acid composition than found in control plants with a concomitant increase in polar lipids may favor seedling growth during Cd exposure. However, the observed increase in the steryl lipid （SL）： phospholipid （PL） ratio （twofold）, the decrease in monogalactosyldiacylglycerol （MGDG）： DGDG ratio, as well as the induction of lipid peroxidation in roots may represent symptoms of membrane injury. In shoots, the unsaturation level was markedly decreased in PC and phosphatidylglycerol （PG） after Cd exposure, but showed a significant increase in sulfoquinovosyldiacylglycerol （SQDG）, MGDG and DGDG. The content of PG and MGDG was decreased by about 65%, while PC accumulated to higher levels （4.4-fold increase）. Taken together, these changes in the polar lipid unsaturation and composition are likely to be due to alterations in the glycerolipid pathway. These results also support the idea that the increase in overall unsaturation plays some role in enabling the plant to withstand the metal exposure.

关键词： cadmium fatty acids heavy metal stress lipid peroxidation polar lipids unsaturation level Zea mays.

Hip joint center localisation: A biomechanical application to hip arthroplasty population

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World Journal of Orthopedics 2012年第8期3卷 131-136页

作者： Vicky Bouffard Mickael Begon Annick Champagne Payam Farhadnia Pascal-André Vendittoli Martin Lavigne Franois Prince Department of Kinesiology University of Montreal laboratoire de bio-ingénierie du mouvement Laval Campus University of Montreal Département d’orthopédie Maisonneuve-Rosemont Hospital

AIM: To determine hip joint center(HJC) location on hip arthroplasty population comparing predictive and functional approaches with radiographic ***: The distance between the HJC and the mid-pelvis was calculated and compared between the three approaches. The localisation error between the predictive and functional approach was compared using the radiographic measurements as the reference. The operated leg was compared to the non-operated ***: A significant difference was found for the distance between the HJC and the mid-pelvis when comparing the predictive and functional method. The functional method leads to fewer errors. A statistical difference was found for the localization error between the predictive and functional method. The functional method is twice more ***: Although being more individualized, the functional method improves HJC localization and should be used in three-dimensional gait analysis.

关键词： Hip arthroplasty Hip joint center localization Predictive approach Functional approach Radiographic measurements

PGRL1 and LHCSR3 Compensate for Each Other in Controlling Photosynthesis and Avoiding Photosystem I Photoinhibition during High Light Acclimation of Chlamydomonas Cells

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Molecular Plant 2017年第1期10卷 216-218页

作者： Frédéric Chaux Xenie Johnson Pascaline Auroy Audrey Beyly-Adriano Isabelle Te Stéphan Cuiné Gilles Peltier CEA CNRS Aix-Marseille Université Institut de Biosciences et Biotechnologies Aix-Marseille UMR 7265 Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues CEA Cadarache Saint-Paul-lez-Durance 13108 France

Dear Editor,In natural environments, photosynthetic organisms experience frequent changes in the light supply, requiring modulation of light harvesting and electron transfer reactions to avoid mismatch between light conversion and metabolic reactions that may result in the production of harmful reactive oxygen species. They have thus evolved photoprotective regulatory mechanisms including the dissipation of excess energy （or non-photochemical quench-ing, NPQ）,

关键词： Controlling Photoinhibition Chlamydomonas

Tribological Behavior of CoCr28Mo/CoCr28Mo Pair in the Presence of bio-lubrication

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Journal of bionic Engineering 2018年第4期15卷 703-712页

作者： Manel Guezmil Walid Bensalah Salah Mezlini laboratoire de Genie Mecanique (LGM) ENIM Rue Ibn Eljazzar 5019 Monastir University Tunisia

The tribological behavior of the CoCr28Mo/CoCr28Mo pair was studied using pin-on-disc tribometer. Four bio-lubricants were re- tained including pure sesame oil, nigella oil, physiological solution （0.9% NaC1） and Hyalgan. Hyalgan is a sodium hyaluronate （20 mg/2 mL） pharmaceutical intra-articular injection used, in this study, as a reference. The Coefficient of Friction （CoF） and wear rate （K） of CoCr28Mo were computed after tribological tests. The tribological efficiency of vegetable oils with regard to their chemical compo- sition and structure was compared to Hyalgan. It is found that, the use of vegetable oils enhanced significantly the tribological behavior of CoCr28Mo/CoCr28Mo pair. The obtained results were correlated to the chemical structure of vegetable oils namely the Unsaturations Number （UN） of fatty acids constituting the oil. Microscopic and chemical characterization of CoCr28Mo pins and discs were conducted and the wear mechanism was discussed.

关键词： CoCr alloy friction coefficient wear resistance oil lubrication

Characterization of dominant giant rod-shaped magnetotactic bacteria from a low tide zone of the China Sea

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Journal of Oceanology and Limnology 2018年第3期36卷 783-794页

作者： TENG Zhaojie ZHANG Wenyan CHEN Yiran PAN Hongmiao XIAO Tian WU Long-Fei CAS Key Laboratory of Marine Ecology ＆ Environmental Sciences Institute of OceanologyChinese Academy of Sciences Qingdao 266071China University of Chinese Academy of Sciences Beijing 100049China Laboratory of Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and Technology Qingdao 266000China laboratoire de Chimie Bacterienne UMR7283Institut de Microbiologie de la MediterraneeAix-Marseille UniversiteCNRS MarseilleFrance laboratoire International Associe de la bio-Mineralisation et Nano-Structures CNRSMarseilleFrance

Magnetotactic bacteria are a group of Gram-negative bacteria that synthesize magnetic crystals, enabling them to navigate in relation to magnetic field lines. Morphologies of magnetotactic bacteria include spirillum, coccoid, rod, vibrio, and multicellular morphotypes. The coccid shape is generally the most abundant morphotype among magnetotactic bacteria. Here we describe a species of giant rod-shaped magnetotactic bacteria(designated QR-1) collected from sediment in the low tide zone of Huiquan Bay(Yellow Sea, China). This morphotype accounted for 90% of the magnetotactic bacteria collected, and the only taxonomic group which was detected in the sampling site. Microscopy analysis revealed that QR-1 cells averaged(6.71±1.03)×(1.54±0.20) m m in size, and contained in each cell 42–146 magnetosomes that are arranged in a bundle formed one to four chains along the long axis of the cell. The QR-1 cells displayed axial magnetotaxis with an average velocity of 70±28 mm/s. Transmission electron microscopy based analysis showed that QR-1 cells had two tufts of fl agella at each end. Phylogenetic analysis of the 16 S r RNA genes revealed that QR-1 together with three other rod-shaped uncultivated magnetotactic bacteria are clustered into a deep branch of A lphaproteobacteria.

关键词： Alphaproteobacteria flagella motility rod-shaped magnetotactic bacteria

Antioxidant and antimicrobial activities of selected medicinal plants from Algeria

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Journal of Coastal Life Medicine 2014年第6期 478-483页

作者： Krimat Soumia Dob Tahar Lamari Lynda Boumeridja Saida Chelghoum Chabane Metidji Hafidha laboratoire de Molécules bio-Actives et Valorisation de la biomasse ENS koubaAlgeria laboratoire de biologie des Systèmes Microbiens ENSKoubaAlgeria Departement of Natural sciences ENSKoubaAlgeria laboratoire de chromatographie facultéde chimieUSTHBAlgiersAlgeria

Objective:To evaluate the antioxidant and antimicrobial activity of methanolic extract extracts of selected Algerian medicinal ***:Antioxidant activity of extracts was evaluated in terms of radical scavenging potential(2,2-diphenyl-1-picrylhydrazyl)and β-carotene bleaching *** phenolic contents and flavonoid contents were also *** activity of these plants was examined against Bacillus subtilis,Staphylococcus aureus,Escherichia coli,Pseudomonas aeruginosa and Candida ***:The values of IC_(50) ranged from 4.30μg/mL to 486.6μg/mL for the DPPH method,while total antioxidant activity using β-carotene/linoleic acid bleaching assay ranged from 17.03%to 86.13%.It was found that Pistacia lentiscus showed the highest antioxidant capacities using DPPH assay(IC_(50)=4.30μg/mL),while Populus trimula,Origanum glandulosum,Centaurea calcitrapa,Sysimbrium officinalis and Rhamnus alaternus showed the highest percent of total antioxidant activity in β-carotene/linoleic acid bleaching *** phenolic and flavonoid contents ranged from 3.96 to 259.65 mg GAE/g extract and from 1.13 to 26.84 mg QE/g extract,*** most interesting antimicrobial activity was obtained from Sysimbrium officinalis,Rhamnus alaternus,Origanum glandulosum,Cupressus sempervirens,Pinus halipensis and Centaurea ***:The results indicated that the plants tested may be potential sources for isolation of natural antioxidant and antimicrobial compounds.

关键词： Antioxidant activity DPPH Antimicrobial activity Disc diffusion method Total phenolic content Total flavonoid content

A transcriptional cascade mediated by two APETALA2 family members orchestrates carotenoid biosynthesis in tomato

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Journal of Integrative Plant biology 2024年第6期66卷 1227-1241页

作者： Xiaoqing He Kaidong Liu Yi Wu Weijie Xu Ruochen Wang Julien Pirrello Mondher Bouzayen Mengbo Wu Mingchun Liu Key Laboratory of bio-Resource and Eco-Environment of Ministry of Education College of Life SciencesSichuan UniversityChengdu 610065China Life Science and Technology School Lingnan Normal UniversityZhanjiang 524048China laboratoire de Recherche en Sciences Vegetales—Genomique et biotechnologie des Fruits—UMR5546 Universite de ToulouseCNRSUPSToulouse-INPToulouse 31013France

Carotenoids are important nutrients for human health that must be obtained from plants since they cannot be biosynthesized by the human *** the regulatory mechanism of carotenoid metabolism in plants represents the first step toward manipulating carotenoid contents in plants by molecular design *** this study,we determined that SlAP2c,an APETALA2(AP2)family member,acts as a transcriptional repressor to regulate carotenoid biosynthesis in tomato(Solanum lycopersicum).Knockout of SlAP2c in both the“Micro Tom”and“Ailsa Craig”backgrounds resulted in greater lycopene accumulation,whereas overexpression of this gene led to orange-ripe fruit with significantly lower lycopene contents than the wild *** established that SlAP2c represses the expression of genes involved in lycopene biosynthesis by directly binding to the cis-elements in their ***,SlAP2c relies on its EAR motif to recruit the co-repressors TOPLESS(TPL)2/4 and forms a complex with histone deacetylase(had)1/3,thereby reducing the histone acetylation levels of lycopene biosynthesis ***,SlAP2a,a homolog of SlAP2c,acts upstream of SlAP2c and alleviates the SlAP2c-induced repression of lycopene biosynthesis genes by inhibiting SlAP2c transcription during fruit ***,we identified a transcriptional cascade mediated by AP2 family members that regulates lycopene biosynthesis during fruit ripening in tomato,laying the foundation for the manipulation of carotenoid metabolism in plants.

关键词： APETALA2 family members carotenoid biosynthesis tomato transcriptional cascade

The Contribution of the Dromedary in the Spontaneous Plant Seeds Transfer in the Northern Algerian Sahara

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Journal of Life Sciences 2012年第3期6卷 300-303页

作者： Trabelsi Hafida Senoussi Abdelhakim Chehma Abdelmadjid Faye Bernard laboratoire de bio-ressources Sahariennes Preservation et Valorisation Universite Kasdi Merbah Ouargla 30000 Algerie CIRAD-ES Montpellier Cedex 34398 France

The current study focuses on the contribution of the dromedary in the preservation and increase of spontaneous flora through seeds transfer by endozoochory. Thus dromedary faeces have been collected in selected area of region of Ghardaia （Northern Algeria Sahara）, since it is one of the three known cameline rangelands during the four successive seasons of the years 2009-2010, in order to examine their seed contents. Our investigation allowed us to invento 1,832 seeds representing 33 different types varying according to the seasons of harvest. The biggest density of seeds was present in summer with 986 seeds, against 424 seeds in winter, 366 seeds in fall and 56 seeds in spring, and of the same way, the biggest number of types was present in summer with 30 types, consistent of the fall season with 26 types, and arrived then the season of winter with 20 types and the one of spring with five types. The gotten results allowed us to appreciate the ecological role of this animal in the desertic ecosystem in the dissemination and the proliferation of the seeds of the spontaneous flora in its fragile and hostile desert environment to the survival of the seeds enveloped in its faeces.

关键词： Dromedary seeds endozoochory Saharan rangelands Gharda'fa.