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Temporal and spatial regulation of biomimetic vascularization in 3D-printed skeletal muscles

BIO-DESIGN AND MANUFACTURING 2024年第5期7卷 597-610页

作者： Jia, Minxuan Fan, Tingting Jia, Tan Liu, Xin Liu, Heng Gu, Qi Chinese Acad Sci Inst Zool Key Lab Organ Regenerat & Reconstruct State Key Lab Membrane Biol Beijing 100101 Peoples R China Beijing Inst Stem cell & Regenerat Med Beijing 100101 Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China Capital Med Univ Beijing Jishuitan Hosp Dept Orthopaed Beijing 100035 Peoples R China Chinese Acad Sci State Key Lab Stem Cell & Reprod Biol Inst Zool Beijing 100101 Peoples R China

In the intricate skeletal muscle tissue, the symbiotic relationship between myotubes and their supporting vasculature is pivotal in delivering essential oxygen and nutrients. This study explored the complex interplay between skeletal muscle and endothelial cells in the vascularization of muscle tissue. By harnessing the capabilities of three-dimensional (3D) bioprinting and modeling, we developed a novel approach involving the co-construction of endothelial and muscle cells, followed by their subsequent differentiation. Our findings highlight the importance of the interaction dynamics between these two cell types. Notably, introducing endothelial cells during the advanced phases of muscle differentiation enhanced myotube assembly. Moreover, it stimulated the development of the vascular network, paving the way for the early stages of vascularized skeletal muscle development. The methodology proposed in this study indicates the potential for constructing large-scale, physiologically aligned skeletal muscle. Additionally, it highlights the need for exploring the delicate equilibrium and mutual interactions between muscle and endothelial cells. Based on the multicell-type interaction model, we can predict promising pathways for constructing even more intricate tissues or organs. [GRAPHICS] .

关键词： Skeletal muscle Vascularization 3D bioprinting cell interaction

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MAD2L2 overexpression attenuates the effects of TNF-α-induced migration and invasion capabilities in colorectal cancer cells

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BIOcell 2024年第9期48卷 1311-1322页

作者： Sun, Haotong Wang, Heying Hao, Yanjie Li, Xin Ling, Jun Wang, Huan Wang, Feimiao Xu, Fang Ningxia Med Univ Sch Basic Med Med Genet & Cell Biol Yinchuan 750004 Ningxia Peoples R China Ningxia Med Univ Key Lab Reprod & Genet Yinchuan 750004 Ningxia Peoples R China

Background: Colorectal cancer is a major global health concern, exacerbated by tumor necrosis factor-alpha (TNF-alpha) and its role in inflammation, with the effects of Mitotic Arrest Deficient 2 Like 2 (MAD2L2) in this context still unclear. Methods: The colorectal carcinoma cell lines HCT116 and SW620 were exposed to TNF-alpha for a period of 24 h to instigate an inflammatory response. Subsequent assessments were conducted to measure the expression of inflammatory cytokines, the activity within the p38 mitogen-activated protein kinase (p38 MAPK) and Phosphoinositide 3-Kinase/AKT Serine/Threonine Kinase pathway (PI3K/AKT) signaling cascades. Transcriptome sequencing and subsequent integrative analysis with the Cancer Genome Atlas (TCGA) program database revealed a significant downregulation of the key factor MAD2L2. Enhancement of MAD2L2 expression was facilitated via lentiviral vector-mediated transfection. The influence of this overexpression on TNF-alpha-prompted inflammation, intracellular signaling pathways, and the migratory and invasive behaviors of the colorectal cancer cells was then scrutinized. Results: TNF-alpha treatment significantly increased the expression of Interleukin-1 beta (IL-113) and Interleukin-6 (IL-6), activated the MAPK p38 and PI3K/AKT signaling pathways, and enhanced cell migration and invasion. A decrease in MAD2L2 expression was observed following TNF-alpha treatment. However, overexpression of MAD2L2 reversed the effects of TNF-alpha, reducing IL-113 and IL-6 levels, attenuating PI3K/AKT pathway activation, and inhibiting cell migration and invasion. Conclusions: Overexpression of MAD2L2 attenuates the pro-inflammatory effects of TNF-alpha, suggesting that MAD2L2 plays a protective role against TNF-alpha-induced migration and invasion of colorectal carcinoma cells. Therefore, MAD2L2 holds potential as a therapeutic target in the treatment of colorectal cancer.

关键词： Colorectal cancer TNF-alpha Migration Invasion TRANSCRIPTION

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Enhanced axonal regeneration and functional recovery of the injured sciatic nerve in a rat model by lithium-loaded electrospun nanofibrous scaffolds

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BIO-DESIGN AND MANUFACTURING 2024年第5期7卷 701-720页

作者： Dolatyar, Banafsheh Zeynali, Bahman Shabani, Iman Tafreshi, Azita Parvaneh Karimi-Soflou, Reza Univ Tehran Coll Sci Sch Biol Dev Biol Lab Tehran *** Iran Natl Inst Genet Engn & Biotechnol Fac Med Biotechnol Dept Mol Med Tehran *** Iran Amirkabir Univ Technol Tehran Polytech Dept Biomed Engn Tehran *** Iran

Increasing evidence indicates that engineered nerve grafts have great potential for the regeneration of peripheral nerve injuries (PNIs). While most studies have focused only on the topographical features of the grafts, we have considered both the biophysical and biochemical manipulations in our applied nanoscaffold. To achieve this, we fabricated an electrospun nanofibrous scaffold (ENS) containing polylactide nanofibers loaded with lithium (Li) ions, a Wnt/beta-catenin signaling activator. In addition, we seeded human adipose-derived mesenchymal stem cells (hADMSCs) onto this engineered scaffold to examine if their differentiation toward Schwann-like cells was induced. We further examined the efficacy of the scaffolds for nerve regeneration in vivo via grafting in a PNI rat model. Our results showed that Li-loaded ENSs gradually released Li within 11 d, at concentrations ranging from 0.02 to (3.64 +/- 0.10) mmol/L, and upregulated the expression of Wnt/beta-catenin target genes (cyclinD1 and c-Myc) as well as those of Schwann cell markers (growth-associated protein 43 (GAP43), S100 calcium binding protein B (S100B), glial fibrillary acidic protein (GFAP), and SRY-box transcription factor 10 (SOX10)) in differentiated hADMSCs. In the PNI rat model, implantation of Li-loaded ENSs with/without cells improved behavioral features such as sensory and motor functions as well as the electrophysiological characteristics of the injured nerve. This improved function was further validated by histological analysis of sciatic nerves grafted with Li-loaded ENSs, which showed no fibrous connective tissue but enhanced organized myelinated axons. The potential of Li-loaded ENSs in promoting Schwann cell differentiation of hADMSCs and axonal regeneration of injured sciatic nerves suggests their potential for application in peripheral nerve tissue engineering. (sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic), (sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(si

关键词： Stem cell Schwann cell differentiation Electrospun nanofibrous scaffold Lithium ion Nerve regeneration CATENIN SIGNALING PATHWAY WNT/BETA-CATENIN STEM-cellS POLYAMIDE FILMS DIFFERENTIATION REMYELINATION DEGENERATION INHIBITION EXPRESSION CHLORIDE

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Deer antler stem cell niche: An interesting perspective

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World Journal of Stem cells 2024年第5期16卷 479-485页

作者： Claudia Cavallini Elena Olivi Riccardo Tassinari Chiara Zannini Gregorio Ragazzini Martina Marcuzzi Valentina Taglioli Carlo Ventura National lab.ratory of Molecular biol.gy and Stem cell Engineering National Institute of Biostructures and Biosystems-Eldor LabBologna 40128Italy Eldor lab Bologna 40128Italy Department of Medical and Surgical Sciences(DIMEC) University of BolognaBologna 40138Italy

In recent years,there has been considerable exploration into methods aimed at enhancing the regenerative capacity of transplanted and/or tissue-resident ***,in particular,have garnered significant interest for their potential to serve as natural scaffolds for *** this editorial,we provide commentary on the study by Wang et al,in a recently published issue of World J Stem cells,which investigates the use of a decellularized xenogeneic extracellular matrix(ECM)derived from antler stem cells for repairing osteochondral defects in rat knee *** focus lies specifically on the crucial role of biol.gical scaffolds as a strategy for augmenting stem cell potential and regenerative capabilities,thanks to the establishment of a favorable microenvironment(niche).Stem cell differen-tiation heavily depends on exposure to intrinsic properties of the ECM,including its chemical and protein composition,as well as the mechanical forces it can ***,these physicochemical cues contribute to a bio-instructive signaling environment that offers tissue-specific guidance for achieving effective repair and *** interest in mechanobiol.gy,often conceptualized as a form of“structural memory”,is steadily gaining more validation and momen-tum,especially in light of findings such as these.

关键词： Extracellular matrix Antler stem cells Stem cell niche Regenerative medicine Decellularized scaffolds cell memory

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Synergistic enhancement of chemotherapy for bladder cancer by photothermal dual-sensitive nanosystem with gold nanoparticles and PNIPAM

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Chinese Chemical Letters 2024年第8期35卷 471-474页

作者： Xiangqian Cao Chenkai Yang Xiaodong Zhu Mengxin Zhao Yilin Yan Zhengnan Huang Jinming Cai Jingming Zhuang Shengzhou Li Wei Li Bing Shen Department of Urology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China Department of Nanomedicine&Shanghai Key lab.of cell Engineering Naval Medical UniversityShanghai 200433China

Bladder cancer is a common malignant tumor of the urinary system with the potential to be treated by nano drug delivery *** current work describes the synthesis and characterization of a novel nanomaterial to construct a nano-carrier based on 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphatecholine(POPC)loaded doxorubicin(DOX)and embedded with gold nanoparticles and poly(N-isopropyl acry-lamide)(PNIPAM)(GNPS@PNIPAM-POPC-DOX,GPPD).The dual-sensitive nanosystem gives simultaneous photothermal treatment and chemotherapy for bladder *** vitro and in vivo properties were as-sessed using bladder cancer cell lines and mice and GPPD system distribution,tumor inhibition,and biocompatibility are *** system had favorable stability,low biol.gical toxicity,controlled re-lease efficiency,photothermal synergistic action,efficient photothermal transition,and favorable tumor suppressive *** a result,GPPD is a potential therapeutic approach for bladder cancer.

关键词： Doxorubicin Gold nanoparticles Thermosensitive polymer Photothermal therapy Bladder cancer

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Temperature-dependent solubility of Rebaudioside A in methanol/ ethanol and ethyl acetate mixtures: Experimental measurements and thermodynamic modeling

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CHINESE JOURNAL OF CHEMICAL ENGINEERING 2024年第8期72卷 164-176页

作者： Liu, Zhenguang Ding, Zexiang Cao, Yifeng Liu, Baojian Yang, Qiwei Zhang, Zhiguo Ren, Qilong Bao, Zongbi Zhejiang Univ Coll Chem & Biol Engn Key Lab Biomass Chem Engn Minist Educ Hangzhou 310058 Peoples R China Inst Zhejiang Univ Quzhou Quzhou 324000 Peoples R China

The equilibrium solubility of Rebaudioside A (Reb A) Form II in binary mixtures of methanol/ethanol and ethyl acetate was quantitatively determined within the temperature range of 283.15-328.15 K at ambient pressure. The experimental findings indicate a positive correlation between the solubility of Reb A (Form II) and both the temperature and the methanol/ethanol content in the solvent system. To describe the solubility data, six distinct models were employed: the modified Apelblat equation, the lh model, the combined nearly ideal binary solvent/Redlich-Kister (CNIBS/R-K) model, the van't HoffJouyban-Acree (VJA) model, the Apelblat-Jouyban-Acree (AJA) model, and the non-random two-liquid (NRTL) model. The combined nearly ideal binary solvent/Redlich-Kister model exhibited the most precise fit for solubility in methanol + ethyl acetate mixtures, reflected by an average relative deviation (ARD) of 0.0011 and a root mean square deviation (RMSD) of 12x10(-7). Conversely, for ethanol + ethyl acetate mixtures, the modified Apelblat equation provided a superior correlation (ARD = 0.0014, RMSD = 4x10(-7)). Furthermore, thermodynamic parameters associated with the dissolution of Reb A (Form II), including enthalpy, entropy, and the Gibbs energy change, were inferred from the data. The findings underscore that the dissolution process is predominantly endothermic across the solvent systems examined. Notably, the entropy changes appear to have a significant influence on the Gibbs free energy associated with the dissolution of Reb A (Form II), suggesting that entropic factors may play a pivotal role in the studied systems. (c) 2024 The Chemical Industry and Engineering Society of China, and Chemical Industry Press Co., Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.

关键词： Rebaudioside A Solubility Thermodynamic modeling Dissolution Binary solvent MIXED-SOLVENTS VANILLIC ACID WATER PURE LIQUID PURIFICATION

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Enlarged DNA unwinding by Nme2Cas9 permits a broadened base editing window beyond the protospacer

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Science China(Life Sciences) 2024年第2期67卷 424-427页

作者： Ziting Chen Xiangyang Li Qian Zhang Wenjun Sun Xiaoxuan Song Xia Zhang Xingxu Huang Bo Sun School of Life Science and Technology and Gene Editing Center ShanghaiTech UniversityShanghai 201210China Zhejiang lab HangzhouZhejiang 311121China CAS Center for Excellence in Molecular cell Science Shanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences Beijing 100049China

Dear Editor,Base editing has been developed to be one of the effective CRISPR-based gene editing techniques(Rees and Liu,2018).Base editors(BEs)take advantage of DNAmodifying enzymes fused with a catalytically impaired CRISPR protein to install base *** BE,DNA-modifying enzymes often process nontarget singlestranded DNA(NTS)generated by Cas proteins.

关键词： install base winding

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Vitri fi cation cryopreservation of ligaments based on zwitterionic betaine

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CHINESE JOURNAL OF CHEMICAL ENGINEERING 2024年第8期72卷 1-9页

作者： Zhang, Liming Liu, Xinmeng Li, Haoyue Zhang, Lei Tianjin Univ Frontier Sci Ctr Synthet Biol Dept Biochem Engn Tianjin 300350 Peoples R China Tianjin Univ Sch Chem Engn & Technol Key Lab Syst Bioengn MOE Tianjin 300350 Peoples R China Haihe lab.Sustainable Chem Transformat Tianjin 300192 Peoples R China

Ligament cryopreservation enables a prolonged shelf life of allogeneic ligament grafts, which is fundamentally important to ligament reconstruction. However, conventional cryopreservation techniques fail to eliminate the damage caused by ice crystal growth and the toxicity of cryopreservation agents (CPAs). Here, we report a novel CPA vitrification formulation primarily composed of betaine for ligament cryopreservation. Comprehensive optimization was conducted on the methods for vitrification and rewarming, as well as the loading and unloading conditions, based on the critical cooling rate (CCR), critical warming rate (CWR), and permeation properties of the CPA. Using biomechanical and histological level tests, we demonstrate the superior performance of our method in ligament cryopreservation. After 30 days of vitrification cryopreservation, parameters such as the Young's modulus, tensile stress, denaturation temperature, and glycosaminoglycans content of the ligament remained essentially unchanged. This work pioneers the application of ice-free cryopreservation for ligament and holds great potential for improving the long-term storage of ligament, providing valuable insights for future cryopreservation technique development. (c) 2024 The Chemical Industry and Engineering Society of China, and Chemical Industry Press Co., Ltd. All rights reserved.

关键词： Ligament Betaine Vitrification Cryopreservation INDUCTION MEMBRANE

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Inter-chromosomal insertions into wild-type chromosomes induced by SCRaMbLE

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FRONTIERS OF CHEMICAL SCIENCE AND ENGINEERING 2024年第9期18卷 107-0页

作者： Zhou, Sijie Li, Junyanrui Cui, Xichen Wang, Ying Yuan, Ying-Jin Tianjin Univ Sch Chem Engn & Technol Frontiers Sci Ctr Synthet Biol Tianjin 300072 Peoples R China Tianjin Univ Sch Chem Engn & Technol Key Lab Syst Bioengn Minist Educ Tianjin 300072 Peoples R China Tianjin Univ Frontiers Res Inst Synthet Biol Tianjin 300072 Peoples R China

Genomic rearrangements play a crucial role in shaping biol.gical phenotypic diversity and driving species evolution. Synthetic chromosome rearrangement and modification by LoxP-mediated evolution (SCRaMbLE) has been applied to explore large-scale genomic rearrangements, yet it has been observed that these rearrangements occur exclusively in genomic regions containing loxPsym sites. Here, we found that SCRaMbLE of synthetic yeast harboring synthetic chromosome V and X can generate a variety of synthetic segment insertions into wild-type chromosomes, ranging from 1 to 300 kb. Furthermore, it was revealed that the novel insertions impacted the transcriptional level of neighboring regions and affected the production of exemplar pathway of zeaxanthin. Collectively, our results improve the understanding of the ability of SCRaMbLE to generate complex structural variations in nonsynthetic regions and provide a potential model to explore genomic transposable events.

关键词： genomic rearrangements synthetic yeast genome SCRaMbLE inter-chromosomal insertions synthetic biol.gy GENOME RESISTANCE EXPRESSION YEAST DNA

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Single-cell RNA-seq reveals the transcriptional program underlying tumor progression and metastasis in neuroblastoma

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FRONTIERS OF MEDICINE 2024年第4期18卷 690-707页

作者： Nian, Zhe Wang, Dan Wang, Hao Liu, Wenxu Ma, Zhenyi Yan, Jie Cao, Yanna Li, Jie Zhao, Qiang Liu, Zhe Tianjin Med Univ Sch Basic Med Sci Dept Immunol Tianjin 300070 Peoples R China Hangzhou Normal Univ Sch Basic Med Sci Dept Cell Biol Zhejiang Key Lab Med Epigenet Hangzhou 311121 Peoples R China Nanjing Med Univ Collaborat Innovat Ctr Canc Personalized Med Nanjing 211166 Peoples R China Tianjin Med Univ Canc Inst & Hosp Depr Pediat Oncol Natl Clin Res Ctr Canc Tianjins Clin Res Ctr CancKey Lab Canc Prevent & Tianjin 300060 Peoples R China

Neuroblastoma (NB) is one of the most common childhood malignancies. Sixty percent of patients present with widely disseminated clinical signs at diagnosis and exhibit poor outcomes. However, the molecular mechanisms triggering NB metastasis remain largely uncharacterized. In this study, we generated a transcriptomic atlas of 15 447 NB cells from eight NB samples, including paired samples of primary tumors and bone marrow metastases. We used time-resolved analysis to chart the evolutionary trajectory of NB cells from the primary tumor to the metastases in the same patient and identified a common 'starter' subpopulation that initiates tumor development and metastasis. The 'starter' population exhibited high expression levels of multiple cell cycle-related genes, indicating the important role of cell cycle upregulation in NB tumor progression. In addition, our evolutionary trajectory analysis demonstrated the involvement of partial epithelial-to-mesenchymal transition (p-EMT) along the metastatic route from the primary site to the bone marrow. Our study provides insights into the program driving NB metastasis and presents a signature of metastasis-initiating cells as an independent prognostic indicator and potential therapeutic target to inhibit the initiation of NB metastasis.

关键词： single-cell RNA sequencing metastasis neuroblastoma epithelial-to-mesenchymal transition MESENCHYMAL TRANSITION TGF-BETA MYCN IDENTIFICATION EXPRESSION PLASTICITY PHENOTYPES DYNAMICS

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