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Glomerular organization in the antennal lobe of the oriental armyworm Mythimna separata

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Journal of Integrative Agriculture 2024年第11期23卷 3812-3829页

作者： Baiwei Ma Qi Chen Xi Chu Yidong Zuo Jiayu Wang Yi Yang Guirong Wang Bingzhong Ren Jilin Provincial Key laboratory of Animal Resource Conservation and Utilization School of Life Sciences Northeast Normal University Key laboratory of Vegetation Ecology Ministry of Education Northeast Normal University State Key laboratory for Biology of plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Chemosensory lab Department of Psychology Norwegian University of Science and Technology Shenzhen Branch Guangdong Laboratory for Lingnan Modern Agriculture Synthetic Biology Laboratory of the Ministry of Agriculture and Rural Affairs Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences

In insects, the number and specificity of antennal lobe glomeruli often reflect the number and diversity of expressed chemosensory genes, which are linked to its ecological niche and specific olfactory needs. The oriental armyworm, Mythimna separata, is an important and common lepidopteran pest of cereal crops. Given its reliance on the olfactory system for crucial behaviors, understanding the evolutionary potential of this system requires a thorough characterization of the anatomical structure of the primary olfactory center. Here, we systematically identified all antennal lobe glomeruli of M. separata based on synaptic antibody immunostaining and mass staining of the olfactory sensory neurons. A total of 69 glomeruli were identified in females and 65 in males, and an intuitive nomenclature based on glomerular positions was applied. Our findings uncovered some sex-specific glomeruli in this species. There were ten female-specific glomeruli and three male-specific glomeruli, except for the macroglomerular complex(MGC) units, with a notable observation that the female labial pit organ glomerulus was larger than its male counterpart. Additionally, we identified four antennal-lobe tracts(ALTs) and retrograde labeling from the calyx revealed that all glomeruli were innervated by the medial ALT projection neurons. The comparison of the olfactory system structures between M. separata and sympatric moths supports their evolutionary convergence in noctuid moths. These results collectively lay the foundation for future studies on olfactory processing in M. separata.

关键词： antennal lobe glomerulus olfactory sensory neuron projection neuron antennal-lobe tract Mythimna separata

Postulation of Leaf Rust Resistance Genes in Seven Chinese Spring Wheat Cultivars

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Journal of Integrative Agriculture 2013年第9期12卷 1580-1588页

作者： SHI Li-hong ZHANG Na HU Ya-ya WEI Xue-jun YANG Wen-xiang LIU Da-qun Department of plant pathology College of Plant Protection/Biological Control Center for Plant Diseases and Plant Pests of HebeiAgricultural University of Hebei

To detect the leaf rust resistance genes in the 7 Chinese spring wheat clultivars Shenmian 99025, Shenmia 99042, Shenmian 85, Shenmian 91, Shenmian 96, Shenmian 1167 and Shenmian 962, Thatcher, Thatcher backgrounded near-isogenic lines and 15 pathotypes of P. triticina were used for gene postulate at the seedling stage, and 9 of the 15 pathotypes were used in the field tests. Molecular markers closely linked to, or co-segregated with resistance genes Lrl, Lr9, LrlO, Lrl9, Lr20, Lr21, Lr24, Lr26, Lr28, Lr29, Lr32, Lr34, Lr35, Lr37, Lr38, and Lr47 were screened to assist detection of the resistance genes. As results, 4 known resistance genes, including Lrl, Lr9, Lr26, and Lr34, and other unknown resistance genes were postulated singly or in combination in the tested cultivars. Shenmian 85, Shenmian 91, Shenmian 96, Shenmian 962, Shenmian 1167, and Shenmian 99042 are potentially useful for wheat production and breeding programs. The result suggested that combining gene postulation, molecular markers and pedigrees is effective and more accuracy method to know the resistance genes in cultivars.

关键词： gene postulation molecular marker Triticum aestivum Puccinia triticina resistance gene

Field evaluation of Streptomyces rubrogriseus HDZ-9-47 for biocontrol of Meloidogyne incognita on tomato

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Journal of Integrative Agriculture 2017年第6期16卷 1347-1357页

作者： JIN Na XUE Hui LI Wen-jing WANG Xue-yan LIU Qian LIU Shu-sen LIU Pei ZHAO Jian-lon JIAN Heng Department of plant pathology China Agricultural University/Key Laboratory of Plant Pathology Ministry of Agriculture Beijing 100193 P.R.China

Streptomyces rubrogriseus HDZ-9-47,isolated from eggs of Meloidogyne spp.,was evaluated as a potential biocontrol agent of Meloidogyne incognita under in vitro and protective *** observations showed that HDZ-9-47 parasitized eggs of *** within 7 *** vitro,the culture filtrate of HDZ-9-47 caused 97.0%mortality of second-stage juveniles（J2s）of *** and inhibited more than 50%egg *** the field,compared with the control,the rootknot index and J2s density in the treatment of drench the broth contained 1012 HDZ-9-47 spores were respectively reduced by 51.1 and 80.7%at 90 days post transplantation,which were better than that in other application doses and *** addition,reduction rates of root-knot index and J2s density of the treatment of combined application of HDZ-9-47 with biofumigation was 87.1 and 91.0%,respectively,better than either of HDZ-9-47 or biofumigation used alone or fosthiazate *** tomato yield also increased by 16.1%.Together,our results suggest that HDZ-9-47 could be an effective biocontrol agent of ***,and that application of HDZ-9-47 combined with cabbage residue biofumigation was a promising and sustainable option for *** control.

关键词： Meloidogyne incognita Streptomyces rubrogriseus biocontrol parasitism nematicidal metabolites control efficacy

Transcriptional landscape of cotton roots in response to salt stress at single-cell resolution

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plant Communications 2024年第2期5卷 46-66页

作者： Pengtao Li Qiankun Liu Yangyang Wei Chaozhu Xing Zhongping Xu Fang Ding Yuling Liu Quanwei Lu Nan Hu Tao Wang Xiangqian Zhu Shuang Cheng Zhaoguo Li Zilin Zhao Yanfang Li Jiangping Han Xiaoyan Cai Zhongli Zhou Kunbo Wang Baohong Zhang Fang Liu Shuangxia Jin Renhai Peng College of Biology and Food Engineering Anyang Institute of TechnologyAnyangHenan 455000China State Key laboratory of Cotton Biology Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyangHenan 455000China Research Base Anyang Institute of TechnologyState Key Laboratory of Cotton BiologyAnyang 455000HenanChina School of Life Sciences Zhengzhou UniversityZhengzhouHenan 450001China Hubei Hongshan laboratory National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanHubeiChina Hubei Key laboratory of plant pathology Huazhong Agricultural UniversityWuhanHubei 430070China Department of Biology East Carolina UniversityGreenvilleNC 27858USA

Increasing soil salinization has led to severe reductions in plant yield and quality,and investigating the mo-lecular mechanism of salt stress response is therefore an urgent *** this study,we systematically analyzed the response of cotton roots to salt stress using single-cell transcriptomics technology;56281 high-quality cells were obtained from 5-day-old lateral root tips of Gossypium arboreum under natural growth conditions and different salt *** cell types with an array of novel marker genes were identified and confirmed by in situ RNA hybridization,and pseudotime analysis of some specific cell types revealed their potential differentiation *** changes in cell numbers under salt stress were observed for outer epidermal and inner endodermal cells,which were significantly enriched in response to stress,amide biosynthetic process,glutathione metabolism,and glycolysis/*** of differentially expressed genes identified in multiple comparisons revealed other functional ag-gregations concentrated on plant-type primary cell wall biogenesis,defense response,phenylpropanoid biosynthesis,and metabolic *** candidate differentially expressed genes encoding transcrip-tion factors or associated with plant hormones also responsive to salt stress were identified,and the func-tion of Ga03G2153,annotated as auxin-responsive GH3.6,was confirmed by virus-induced gene *** GaGH3.6-silenced plants showed a severe stress-susceptible phenotype,and physiological and biochemical measurements indicated that they suffered more significant oxidative *** results suggest that GaGH3.6 might participate in cotton salt tolerance by regulating redox *** thus construct a transcriptional atlas of salt-stressed cotton roots at single-cell resolution,enabling us to explore cellular heterogeneity and differentiation trajectories and providing valuable insights into the mo-lecular mechanisms that underlie plan

关键词： cotton salt stress scRNA-seq root cell types virus-induced gene silencing

Parasitism and pathogenicity of Radopholus similis to Ipomoea aquatica, Basella rubra and Cucurbita moschata and genetic diversity of different populations

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Journal of Integrative Agriculture 2016年第1期15卷 120-134页

作者： LI Yu WANG Ke XIE Hui XU Chun-ling WANG Dong-wei LI Jing HUANG Xin PENG Xiao-fang laboratory of plant Nematology and Research Center of Nematodes of plant Quarantine Department of Plant Pathology South China Agricultural University

Ten populations of Radopholus similis from different ornamental hosts were tested for their parasitism and pathogenicity to water spinach （Ipomoea aquatic）, malabar spinach （Basella rubra）, and squash （Cucurbita moschata） in pots. The re- sults showed all three plants were new hosts of R. similis. Growth parameters of plants inoculated with nematodes were significantly lower than those of healthy control plants. All R. similis populations were pathogenic to the three plants, but pathogenicity differed among populations from different hosts. The same R. similis populations also showed different pathogenic effects in the three different plants. RadN5 population from Anthurium andraeanum had the highest pathoge- nicity to the three studied plants. RadN1 from A. andraeanum had the lowest pathogenicity to squash and RadN7 from Chrysalidocarpus lutesens had the lowest pathogenicity to water spinach and malabar spinach. R. similis is usually associated with root tissues, but here we report that it could be found to move and feed in the stem bases of all three studied plants. Sequence and phylogenetic analyses of DNA markers of the 18S rRNA, 28S rRNA, ITS rRNA, and mitochondrial DNA gene sequences of ten R. similis populations revealed significant genetic diversity. RadN5 and RadN6 populations from anthurium showed a close genetic relationship and could be distinguished from other populations by PCR-RFLP. At the same time, RadN5 and RadN6 populations were the most pathogenic to three studied plants. These results confirm the existence of large biological variability and molecular diversity among R. similis populations from the same or different hosts, and these characteristics are related to pathogenic variability.

关键词： burrowing nematode parasitism pathogenicity genetic diversity PCR-RFLP

Strong Suppression of Systemic Acquired Resistance in Arabidopsis by NRR is Dependent on its Ability to Interact with NPRland its Putative Repression Domain

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Molecular plant 2008年第3期1卷 552-559页

作者： Mawsheng Chern Patrick E. Canlas Pamela C. Ronald Department of plant pathology University of California Davis CA 95616 USA

Systemic Acquired Resistance （SAR） in plants confers lasting broad-spectrum resistance to pathogens and requires the phytohormone salicylic acid （SA）. Arabidopsis NPR1/NIM1 is a key regulator of the SAR response. Studies attempting to reveal the function of NPR1 and how it mediates SA signaling have led to isolation of two classes of proteins that interact with NPRI＂ the first class includes rice NRR, Arabidopsis NIMIN1, NIMIN2, and NIMIN3, and tobacco NIMIN2-1ike proteins; the second class belongs to TGA transcription factors. We have previously shown that overexpression of N RR in rice suppresses both basal and Xa21-mediated resistance. In order to test whether NRR affects SA-induced, NPRl-mediated SAR, we have transformed Arabidopsis with the rice NRR gene and tested its effects on the defense response. Expression of NRR in Arabidopsis results in suppression of PR gene induction by SAR inducer and resistance to pathogens. These phenotypes are even more severe than those of the nprl-1 mutant. The ability of NRR to suppress PR gene induction and disease resistance is correlated with its ability to bind to NPR1 because two point mutations in NRR, which reduce NPR1 binding, fail to suppress NPR1. In contrast, wild-type and a mutant NRR, which still binds to NPR1 strongly, retain the ability to suppress the SAR response. Replacing the C-terminal 79 amino acids of NRR with the VP16 activation domain turns the fusion protein into a transcriptional co-activator. These results indicate that NRR binds to NPR1 in vivo in a protein complex to inhibit transcriptional activation of PR genes and that NRR contains a transcription repression domain for active repression.

关键词： NRR NPR1 SA SAR disease resistance.

Repeated release of cerium oxide nanoparticles altered algal responses:Growth,photosynthesis,and photosynthetic gene expression

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Eco-Environment & Health 2024年第3期3卷 290-299页

作者： Saibo Liu Jingheng Han Xiaowu Ma Xiaoshan Zhu Han Qu Guorong Xin Xiaochen Huang State Key lab of Biocontrol Guangdong Provincial Key Laboratory of Plant ResourcesSchool of Agriculture and BiotechnologyShenzhen Campus of Sun Yat-sen UniversityShenzhen 518107China College of Ecology and Environment Hainan UniversityHaikou 570228China College of Environment and Ecology Key Laboratory of the Three Gorges Reservoir Region's Eco-EnvironmentMinistry of EducationChongqing UniversityChongqing 400045China

The expanding production of engineered nanomaterials(ENMs)can eventually cause their increased release into and presence in aquatic ecosystems,potentially threatening the health of aquatic organisms and the stability of the ecological ***,ENMs are repeatedly released into real-world aquatic environments in relatively low concentrations,potentially affecting photosynthesis in primary producers such as ***,knowledge regarding the effects of repeated exposure to ENMs on algal photosynthesis is still ***,the physiological responses of the freshwater algae Chlorella vulgaris following single and repeated exposures to cerium oxide nanoparticles(CeO2 NPs)were investigated at 10 mg/L,with a focus on *** results showed that repeated exposures triggered increased photosynthetic pigment contents,oxidative stress levels,decreased photosynthetic performance,and lower biomass in *** compared to a single ***-related genes(i.e.,petA,petB,psaA,atpB,and rbcL)were found to be upregulated following repeated *** for petB,repeated rather than single exposure treatment significantly upregulated its expression levels by 2.92–10.24-fold compared to unexposed ***,increased exposure times could aggravate the interaction between CeO2 NPs and algae,elevating 8.13%,12.13%,and 20.51%Ce distribution on the algal cell surface or intracellularly,compared to a single *** study is the first to investigate the effects of ENM exposure times on algal photosynthesis,providing new insights into the assessment of the risks these materials pose to real-world aquatic environments.

关键词： Cerium oxide nanoparticles Environmental risks Repeated exposures Algae Photosynthesis Gene expression level

3D bioprinting of in vitro porous hepatoma models:establishment,evaluation,and anticancer drug testing

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Bio-Design and Manufacturing 2024年第2期7卷 137-152页

作者： Xiaoyuan Wang Zixian Liu Qianqian Duan Boye Zhang Yanyan Cao Zhizhong Shen Meng Li Yanfeng Xi Jianming Wang Shengbo Sang Shanxi Key laboratory of Micro Nano Sensors and Artificial Intelligence Perception College of Electronic Information and Optical EngineeringTaiyuan University of TechnologyTaiyuan 030024China Key lab of Advanced Transducers and Intelligent Control System of the Ministry of Education Taiyuan University of TechnologyTaiyuan 030024China Shanxi Institute of 6D Artificial Intelligence Biomedical Science Taiyuan 030031China Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering Taiyuan 030024China Department of pathology Shanxi Provincial Cancer HospitalTaiyuan 030024China General Hospital of Taiyuan Iron and Steel Company Taiyuan 030809China

Traditional tumor models do not tend to accurately simulate tumor growth in vitro or enable personalized treatment and are particularly unable to discover more beneficial targeted *** address this,this study describes the use of threedimensional(3D)bioprinting technology to construct a 3D model with human hepatocarcinoma SMMC-7721 cells(3DP-7721)by combining gelatin methacrylate(GelMA)and poly(ethylene oxide)(PEO)as two immiscible aqueous phases to form a bioink and innovatively applying fluorescent carbon quantum dots for long-term tracking of *** GelMA(10%,mass fraction)and PEO(1.6%,mass fraction)hydrogel with 3:1 volume ratio offered distinct pore-forming characteristics,satisfactorymechanical properties,and biocompatibility for the creation of the 3DP-7721 *** analysis and quantitative real-time fluorescence polymerase chain reaction(PCR)were used to evaluate the biological properties of the *** with the two-dimensional culture cell model(2D-7721)and the 3D mixed culture cell model(3DM-7721),3DP-7721 significantly improved the proliferation of cells and expression of tumor-related proteins and ***,we evaluated the differences between the three culture models and the effectiveness of antitumor drugs in the three models and discovered that the efficacy of antitumor drugs varied because of significant differences in resistance proteins and genes between the three *** addition,the comparison of tumor formation in the three models found that the cells cultured by the 3DP-7721 model had strong tumorigenicity in nude *** evaluation of the levels of biochemical indicators related to the formation of solid tumors showed that the 3DP-7721 model group exhibited pathological characteristics of malignant tumors,the generated solid tumors were similar to actual tumors,and the deterioration was *** research therefore acts as a foundation for the application of 3DP-7721 models in drug devel

关键词： 3D bioprinting Hepatoma tumor models Drug screening Antitumor drug development

Cryptic species composition and genetic diversity within Bemisia tabaci complex in soybean in India revealed by mtCOI DNA sequence

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Journal of Integrative Agriculture 2015年第9期14卷 1786-1795页

作者： Prasanna H C Kanakala S Archana K Jyothsna P Varma R K Malathi V G Division of Vegetable Improvement Indian Institute of Vegetable Research Advanced Centre for plant Virology Division of Plant PathologyIndian Agricultural Research Institute Department of plant pathology Jawaharlal Nehru Krishi Vishwavidyalaya

Bemisia tabaci is a cryptic species complex, causing signiifcant loss on many agricultural y important crops worldwide. Knowledge on species composition and diversity within B. tabaci complex is critical for evolving sustainable pest management strategies. Here we investigate the whitelfy species complex in soybean in major soybean growing states of India. The mitochondrial cytochrome oxidase gene subunit-1 (mtCOI) based phylogenetic relationships established using Bayesian methods indicated the existence of three cryptic species namely Asia I, Asia II 1, and Asia II 7. Al the haplotypes detected in the study could be assigned to these three cryptic species fol owing the species demarcation criteria of 3.5%divergence threshold. Of these, Asia II 1 was found to be predominant with wide spread distribution across the surveyed regions from cool temperate zones to hot and humid tropical plains. On the contrary, cryptic species Asia II 7 showed localized distribu-tion. The Asia II 1 exhibited the highest haplotype diversity and Asia I showed high level of nucleotide diversity. There was a signiifcantly high genetic differentiation among these three cryptic species. The MEAM 1, a dreadful invasive species was not detected in the specimens tested in the current study. The diversity and distribution of three cryptic species is discussed in the light of current knowledge on distribution of whitelfy species in India and yel ow mosaic disease observed during sampling survey.

关键词： whitefly mitochondrial cytochrome oxidase gene subunit-1 AsiaⅠ AsiaⅡ 1 AsiaⅡ 7 begomovirus and yellow mosaic disease