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Detection of Schistosoma mansoni DNA using polymerase chain reaction from serum and dried blood spot card samples of an adult population in North-western Tanzania

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Infectious Diseases of Poverty 2021年第1期10卷 66-73页

作者： Antje Fuss Humphrey DMazigo Andreas Mueller Medical Mission Institute Hermann-Schell-Str.797074 WuerzburgGermany Department of Medical Parasitology and Entomology School of MedicineCatholic University of Health and Allied SciencesP.O.Box 1464MwanzaTanzania Department ofTropical Medicine Klinikum Wuerzburg Mitte gGmbHMedical Mission HospitalWuerzburgGermany.

Background:Real-time polymerase chain reaction(PCR)is a sensitive and specific method for diagnosing ***,this method should be performed in a laboratory,usually located distant from the sample collection ***,it is important to have fast sampling preservation methods,which allow simple transport prior to DNA extraction and *** aim of this study was to verify if blood samples applied to filter paper are suitable for analysis of Schistosoma mansoni DNA by real-time ***:A cross-sectional study was conducted among 100 study participants aged 17 to 70 years in a fishing village on the southern shore of Lake Victoria,*** samples and ethylenediaminetetraacetic acid(EDTA)-anticoagulated whole blood for preparation of dried blood spots(DBS)were collected to test for Schistosoma mansoni infection by real-time PCR.A combined diagnostic reference of positive results of serum-based real-time PCR and the Kato-Katz(KK)method was used for *** and negative predictive value(NPV)were *** Wilcoxon signed-rank test was chosen to compare the mean cycle threshold(Ct)values from serum and ***:According to the reference,92.5%*** positive samples were *** serum-based real-time PCR performed excellently with 95.4%sensitivity,whereas the DBS-based real-time PCR showed a low sensitivity(45.4%).The Ct-values were significantly higher in DBS(median:37.3)than in serum samples(median:27.5,P<0.001),reflecting a lower parasite-specific DNA load on the filter *** increasing egg counts,an increase in sensitivity was observed for all *** POC-CCA test and the serum-based real-time PCR showed a sensitivity of 100%for medium and severe *** DBS real-time PCR showed a sensitivity of only 85.7%even for severe ***:DBS-based real-time PCR did not provide good results in our study and therefore should not be recommended or must be tested concerning tempe

关键词： Schistosoma mansoni Real-time PCR Circulating DNA Dried blood spots Tanzania

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Evaluation of serum-based real-time PCR to detect Schistosoma mansoni infection before and after treatment

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Infectious Diseases of Poverty 2020年第3期9卷 114-120页

作者： Antje Fuss Humphrey Deogratias Mazigo Andreas Mueller Medical Mission Institute Hermann-Schell-Str.797074 WuerzburgGermany School of Medicine Department of Medical Parasitology&EntomologyCatholic University of Health and Allied SciencesP.O.Box 1464MwanzaTanzania klinikum wuerzburg mitte ggmbh Medical Mission HospitalDept.of Tropical MedicineWuerzburgGermany

Background:To detect acute schistosomiasis,low-intensity infections,or to verify the success of treatment with praziquantel,highly sensitive test methods are *** aim of this study was therefore to demonstrate the performance of Schistosoma mansoni specific DNA detection in serum and urine using real-time polymerase chain reaction(PCR)in an endemic area before and after ***:The study pursued a 1-week and 20-weeks longitudinal design with a treatment intervention among 36 study participants aged 18 to 70 years in the community of Kayenze,a fishing village in Ilemela district on the southern shore of Lake Victoria in north-western Tanzania between February and June ***,urine and stool samples were collected from each participant to diagnose Schistosoma mansoni infection before and two times after treatment with praziquantel using serum-and urine based real-time PCR,point-of-care circulating cathodic antigen(POC-CCA)rapid diagnostic test and the microscopic Kato-Katz(KK)*** coefficient(κ)was used to estimate the agreement between these diagnostic tests compared to a combined“gold standard”of positive results by serum-based real-time PCR and/or positive egg counts determined by ***’s Tau rank correlation was used to examine the relationship between cycle threshold(Ct)-values and egg counts and the Wilcoxon signed-rank test was used to compare the median Ct-values of the different examination time ***:By using the combined“gold standard”of the parasitological Kato-Katz test and/or serum-based real-time PCR,a *** prevalence of 77.1%could be determined at *** terms of sensitivity,serum-based realtime PCR(96.3%)and POC-CCA assay(77.8%)showed the highest *** detection of DNA from urine samples showed the lowest sensitivity(33.3%).Treatment with praziquantel resulted in a significantly reduced prevalence of *** infection could be detected by Kato-Katz,with the POC-CCA test only 33.3%.The an

关键词： Schistosoma mansoni Real-time PCR Circulating DNA Tanzania

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