Evaluation of serum-based real-time PCR to detect Schistosoma mansoni infection before and after treatment

作     者：Antje Fuss Humphrey Deogratias Mazigo Andreas Mueller Fuss Antje;Deogratias Mazigo Humphrey;Mueller Andreas

作者机构：Medical Mission InstituteHermann-Schell-Str.797074 WuerzburgGermany School of MedicineDepartment of Medical Parasitology&EntomologyCatholic University of Health and Allied SciencesP.O.Box 1464MwanzaTanzania Klinikum Wuerzburg Mitte gGmbHMedical Mission HospitalDept.of Tropical MedicineWuerzburgGermany 

出 版 物：《Infectious Diseases of Poverty》 (贫困所致传染病（英文）)

年 卷 期：2020年第9卷第3期

页      面：114-120页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：This study was funded by the Georg Ludwig Rexroth Foundation(http://www.rexroth-stiftung.de)and German Academ ic Exchange Service(Deutscher Akademischer Austauschdienst DAAD grant number 57141273(httpsy/www.daad.de/de/)) 

主　　题：Schistosoma mansoni Real-time PCR Circulating DNA Tanzania 

摘      要：Background:To detect acute schistosomiasis,low-intensity infections,or to verify the success of treatment with praziquantel,highly sensitive test methods are *** aim of this study was therefore to demonstrate the performance of Schistosoma mansoni specific DNA detection in serum and urine using real-time polymerase chain reaction(PCR)in an endemic area before and after ***:The study pursued a 1-week and 20-weeks longitudinal design with a treatment intervention among 36 study participants aged 18 to 70 years in the community of Kayenze,a fishing village in Ilemela district on the southern shore of Lake Victoria in north-western Tanzania between February and June ***,urine and stool samples were collected from each participant to diagnose Schistosoma mansoni infection before and two times after treatment with praziquantel using serum-and urine based real-time PCR,point-of-care circulating cathodic antigen(POC-CCA)rapid diagnostic test and the microscopic Kato-Katz(KK)*** coefficient(κ)was used to estimate the agreement between these diagnostic tests compared to a combined“gold standardof positive results by serum-based real-time PCR and/or positive egg counts determined by ***’s Tau rank correlation was used to examine the relationship between cycle threshold(Ct)-values and egg counts and the Wilcoxon signed-rank test was used to compare the median Ct-values of the different examination time ***:By using the combined“gold standardof the parasitological Kato-Katz test and/or serum-based real-time PCR,a *** prevalence of 77.1%could be determined at *** terms of sensitivity,serum-based realtime PCR(96.3%)and POC-CCA assay(77.8%)showed the highest *** detection of DNA from urine samples showed the lowest sensitivity(33.3%).Treatment with praziquantel resulted in a significantly reduced prevalence of *** infection could be detected by Kato-Katz,with the POC-CCA test only 33.3%.The an