检索结果-南通市图书馆

Genome editing in plants using the TnpB transposase system

在线全文

学校读者我要写书评

暂无评论

aBIOTECH 2024年第2期5卷 225-230页

作者： Qi Li Yongqiang Wang Zhuoting Hou Hang Zong Xuping Wang Yong EZhang Haoyi Wang Haitao Chen Wen Wang Kang Duan School of Ecology and Environment Northwestern Polytechnical UniversityXi’an 710129China Sanjie Institute of Forage Yangling 712100China key laboratory of zoological Systematics and evolution Institute of ZoologyChinese Academy of SciencesBeijing 100101China State key laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China

The widely used clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated nuclease(Cas)system is thought to have evolved from IS200/IS605 *** proteins,encoded by one type of IS200/IS605 transposon,are considered to be the evolutionary ancestors of Cas12 nucleases,which have been engineered to function as RNA-guided DNA endonucleases for genome editing in bacteria and human *** nucleases,which are smaller than Cas nucleases,have been engineered for use in genome editing in animal systems,but the feasibility of this approach in plants remained ***,we obtained stably transformed genome-edited mutants in rice(Oryza sativa)by adapting three recently identified TnpB genome editing vectors,encoding distinct TnpB nucleases(ISAam1,ISDra2,and ISYmu1),for use in plants,demonstrating that the hypercompact TnpB proteins can effectively edit plant ***2 and ISYmu1 precisely edited their target sequences,with no off-target mutations detected,showing that TnpB transposon nucleases are suitable for development into a new genome editing tool for *** modifications improving the genome-editing efficiency of the TnpB system will facilitate plant functional studies and breeding programs.

关键词： ISAam1 ISDra2 ISYmu1 Plant genome editing Rice TnpB transposon endonuclease

Findings on three endocommensal scuticociliates(Protista,Ciliophora)from freshwater mollusks,including their morphology and molecular phylogeny with descriptions of two new species

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Marine Life Science & Technology 2024年第2期6卷 212-235页

作者： Tao Li Tengyue Zhang Mingjian Liu Zhe Zhang Jiachen Zhang Junhua Niu Xiangrui Chen Saleh A.Al‑Farraj Weibo Song Institute of evolution and Marine Biodiversity Ocean University of China and Key Laboratory of Evolution and Marine Biodiversity(Ministry of Education)Qingdao 266003China The key laboratory of zoological Systematics and Application College of Life SciencesHebei UniversityBaoding 071002China College of Marine Life Sciences Ocean University of ChinaQingdao 266003China School of Marine Sciences Ningbo UniversityNingbo 315800China Zoology Department College of ScienceKing Saud University11451 RiyadhSaudi Arabia laboratory for Marine Biology and Biotechnology Laoshan LaboratoryQingdao 266237China

Species of the ciliate genera Myxophyllum and Conchophthirus are found as endocommensals of terrestrial and freshwater mollusks,*** far,there have been few studies of these genera and morphological data for most members are often *** the present work,two new species,Myxophyllum weishanense *** Conchophthirus paracurtus ***.,and a known species,Conchophthirus lamellidens,were isolated from hosts in Lake Weishan Wetland,*** studies indicate that *** *** be recognized mainly by the combination of about 60 somatic kineties on both ventral and dorsal sides and the presence of caudal *** paracurtus *** from congeners in its body shape and size,having a glabrous area on the posterior right side,and having fewer somatic *** addition,differences in their ITS2(Internally Transcribed Spacer 2)secondary structures support the discrimination of the two new species from their highly similar *** improved diagnosis for the poorly known species,***-lidens is also *** analyses reveal that members of the genus Myxophyllum belong to a fully supported clade that is sister to a large,poorly supported clade consisting of Hemispeiridae,Ancistridae,and several lineages of the nonmonophyletic *** Myxophyllum clade also includes Protophyra ovicola JQ956552,a possible *** of the two new Conchophthirus species cluster with other congeners in a fully supported clade that is unrelated to either the‘typical’thigmotrichs or to pleuronematids,thus conflicting with the traditional classification,and may represent an orphan scuticociliate lineage.

关键词： Ciliates Endocommensal ITS2 secondary structure Mollusks New taxa Wetland

Bird specimen number linked with species trait and climate niche breadth

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Avian Research 2024年第1期15卷 108-115页

作者： Xiaoyu Duan Xiongwei Huang Jingya Zhang Shuo Lv Gang Song Yanping Wang Gang Feng Ministry of Education key laboratory of Ecology and Resource Use of the Mongolian Plateau&Inner Mongolia key laboratory of Grassland Ecology School of Ecology and EnvironmentInner Mongolia UniversityHohhot010020China key laboratory of Marine Biotechnology of Fujian Province Institute of OceanologyCollege of Marine SciencesFujian Agriculture and Forestry UniversityFuzhou350002China key laboratory of zoological Systematics and evolution Institute of ZoologyChinese Academy of SciencesBeijing100101China laboratory of Island Biogeography and Conservation Biology College of Life SciencesNanjing Normal UniversityNanjing210023China

Biological specimens are fundamental for taxonomy and flora/fauna *** importantly,they also play crucial roles in recording environmental impacts on morphology and behavior,which is vital for biodiversity research and ***,there are few systematic studies on the patterns and drivers of bird specimen number at regional *** study is the first attempt to examine the relationships between bird specimen number and species traits as well as climate niche breadth in China,aiming to answer two questions:1)how do species’temperature niche breadth and precipitation niche breadth influence specimen number?2)which trait is most associated with bird specimen number?The associations between bird specimen number and explanatory variables were examined using ordinary least squares,generalized linear models,phylogenetic generalized linear mixed models,and multiple *** results showed that Muscicapidae was the family with the highest specimen number,and Passeriformes was the order with the highest specimen *** specimen number significantly increased with larger temperature niche breadth and precipitation niche *** number was also positively associated with geographic range size,habitat specificity,hunting vulnerability and clutch size,but negatively associated with body *** findings suggest that future bird specimen collection should pay more attention to birds with limited ecological niches,large body sizes,and small clutch *** research enhances the use of bird specimen data to study and preserve biodiversity.

关键词： Biodiversity conservation Biological specimens Bird traits Scientific collection Specimen number Temperature and precipitation

Indian monsoon drove the dispersal of the thoracica group of Scytodes spitting spiders

在线全文

学校读者我要写书评

暂无评论

zoological Research 2024年第1期45卷 152-159页

作者： Yu-Fa Luo Shu-Qiang Li School of Life and Environmental Sciences Shaoxing UniversityShaoxingZhejiang 312000China key laboratory of zoological Systematics and evolution Institute of ZoologyChinese Academy of SciencesBeijing 100101China Southeast Asia Biodiversity Research Institute Chinese Academy of SciencesYezinNay Pyi Taw 05282Myanmar

We examined the global biogeography of the Scytodes thoracica group of spitting spiders based on 23 years of sampling at the species level(61 species in the thoracica group and 84 species of Scytodes)using DNA data from six *** results indicated that the thoracica group initially dispersed from Southeast Asia to East Africa between 46.5 and 33.0 million years ago,and dispersal events intensified between Southeast/South Asia and East/South Africa from the early to late *** timing of these events indicates that Asian-African faunal exchange of the thoracica group was driven by the Indian monsoon,and the pattern of dispersal suggests that colonialization took root when the Indian monsoon shifted from a North-South direction to an East-West direction from the middle Eocene.

关键词： Geological event Climate change Ballooning organism Faunal exchange Species distribution

Avian lower beak is always overlooked:its coordinate role in shaping species-specific beak should not be underestimated

在线全文

学校读者我要写书评

暂无评论

Integrative Zoology 2024年第2期19卷 339-342页

作者： Yalin CHENG Fumin LEI College of Life Science/Hebei Basic Science Center for Biotic Interactions Institute of Life Science and Green DevelopmentHebei UniversityBaodingChina key laboratory of zoological Systematics and evolution Institute of ZoologyChinese Academy of SciencesBeijingChina University of Chinese Academy of Sciences BeijingChina Center for Excellence in Animal evolution and Genetics Chinese Academy of SciencesKunmingChina

The beak is a key evolutionary innovation in the avian tree of *** diversity in morphology shows birds’successful colonization of various ecological niches on the Earth(Pigot et al.2020),and its morphological plasticity can also enable birds to rapidly respond to changes in environments(Grant&Grant 2006).These natures helped birds survive multiple extinction events(Larson et al.2016).Therefore,investigating the morphological evolution of avian beaks and the underlying developmental and molecular mechanisms will help to decipher the evolution of birds and boost the development of the theory of evolutionary biology.

关键词： coordinate extinction enable

Effects of Dietary Changes on the Gut Microbiota of Cynops orientalis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Herpetological Research 2024年第2期15卷 63-72页

作者： Yuting WANG Yuning XIE Siyu WU Wenwen ZHANG Xiner CHENG Zixuan LI Fangfei HAN Jiayi SHI Yingying SHI Zhirong HE Chunna ZHANG Yixin JIANG Na ZHAO Jiaqi LI Supen WANG College of Life Sciences Anhui Normal UniversityWuhu 241000AnhuiChina Nanjing Institute of Environmental Sciences Ministry of Ecology and Environment of ChinaNanjing 210042JiangsuChina School of Ecology and Environment Anhui Normal UniversityWuhu 241002AnhuiChina key laboratory of zoological Systematics and evolution Institute of ZoologyChinese Academy of SciencesBeijing 100101China

The gut microbiota plays a pivotal role in the maintenance of health for amphibians,and it has been fully recognized,but the effectiveness of various influencing factors has not yet been fully *** this association should be considered while the amphibian order Caudata is facing a severe situation of population decline and extinction,there is little understanding of the association between diets and the diversity of gut microbiota in the amphibian order ***,we conducted an extensive analysis of the gut microbiota of Cynops orientalis fed different diets using functional prediction and 16S rRNA amplicon sequencing ***,we found that wild individuals had greater gut microbial diversity and richness in comparison to captive ***,we identified the bacterial taxa associated with diets and observed differences in the relative abundance of gut microbiota among people on various ***,we have a predictive comprehension of the selection and adaptative significance of shared core ASVs in the gut microbiota in maintaining the healthy survival of *** in a large-scale spatiotemporal *** study emphasizes how diets alter the gut microbiota of Caudata and offers fresh perspectives on the conservation and captive management of species in Caudata.

关键词： 16S rRNA Caudata core microbiota diet gut microbiota

Generation of mitochondrial replacement monkeys by female pronucleus transfer

在线全文

学校读者我要写书评

暂无评论

zoological Research 2024年第2期45卷 292-298页

作者： Chun-Yang Li Xing-Chen Liu Yu-Zhuo Li Yan Wang Yan-Hong Nie Yu-Ting Xu Xiao-Tong Zhang Yong Lu Qiang Sun Institute of Neuroscience Center for Excellence in Brain Science and Intelligence TechnologyState Key Laboratory of NeuroscienceChinese Academy of SciencesShanghai 200031China key laboratory of Genetic evolution and Animal Models Chinese Academy of SciencesKunming 650201China University of Chinese Academy of Sciences Beijing 100049China

Mutations in mitochondrial DNA(mtDNA)are maternally inherited and have the potential to cause severe *** replacement therapies,including spindle,polar body,and pronuclear transfers,are promising strategies for preventing the hereditary transmission of mtDNA *** pronuclear transfer has been used to generate mitochondrial replacement mouse models and human embryos,its application in non-human primates has not been previously *** this study,we successfully generated four healthy cynomolgus monkeys(Macaca fascicularis)via female pronuclear *** individuals all survived for more than two years and exhibited minimal mtDNA carryover(3.8%–6.7%),as well as relatively stable mtDNA heteroplasmy dynamics during *** successful establishment of this nonhuman primate model highlights the considerable potential of pronuclear transfer in reducing the risk of inherited mtDNA diseases and provides a valuable preclinical research model for advancing mitochondrial replacement therapies in humans.

关键词： Non-human primates Mitochondrial replacement Female pronuclear transfer

Thirty-eight spider species (Arachnida: Araneae) from China, Indonesia, Japan and Vietnam

在线全文

学校读者我要写书评

暂无评论

zoological Systematics 2024年第1期49卷 4-98页

作者： Yejie Lin Shuqiang Li Haolin Mo Xihao Wang Institute of Zoology Chinese Academy of SciencesBeijing 100101China key laboratory of Wildlife evolution and Conservation in Mountain Ecosystem of Guangxi Nanning Normal UniversityNanning 530001China Jinan Licheng No.2 High School Jinan 250109China

Four new genera in three spider families are reported, namely Langlibaitiao Lin &Li, gen. nov., of Dictynidae O. Pickard-Cambridge, 1871, with Lathys inaffecta Li, 2017(♂♀,China: Guangxi) as the type species;Huoyanluo Lin & Li, gen. nov., of Macrobunidae Petrunkevitch, 1928, with Huoyanluo ruanxiaoqi Lin & Li, sp. nov.(♂, China: Yunnan) as the type species and Gushangzao Lin & Li, gen. nov. and Gyro Lin & Li, gen. nov., of Theridiidae Sundevall, 1833, with Gushangzao shiqian Lin & Li, sp. nov.(♂♀, China: Xizang) and Gyro zeppeli Lin & Li, sp. nov.(♀, China: Yunnan) as the type species, respectively. Further 31 new species in 19 spider families are reported, including Agelenidae C.L. Koch, 1837:Troglocoelotes doul Lin & Li, sp. nov.(♀, China: Guangxi) and T. ruanxiaowu Lin & Li, ***.(♀, China: Guangxi);Dictynidae: Langlibaitiao zhangshun Lin & Li, sp. nov.(♂, China:Hainan);Hahniidae Bertkau, 1878: Hahnia zhuyifani Lin & Li, sp. nov.(♀, China: Yunnan);Liocranidae Simon, 1897: Platnick xintongi Lin & Li, sp. nov.(♂♀, China: Xizang);Macrobunidae: Funny yanqing Lin & Li, sp. nov.(♂♀, China: Xizang) and Huoyanluo zhangzezhongi Lin & Li, sp. nov.(♂, China: Yunnan);Mysmenidae Petrunkevitch, 1928:Phricotelus yangxiong Lin & Li, sp. nov.(♂♀, China: Chongqing);Nemesiidae Simon, 1889:Raveniola shixiu Lin, Wang & Li, sp. nov.(♀, China: Guangxi) and R. xiezhen Lin, Wang & Li,sp. nov.(♂♀, China: Yunnan);Nesticidae Simon,1894: Speleoticus sicet Lin & Li, sp. nov.(♂♀,China: Sichuan);Ochyroceratidae Fage, 1912: Speocera yiduoi Lin & Li, sp. nov.(♂, China:Guangdong);Oecobiidae Blackwall, 1862: Uroctea chenyui Lin & Li, sp. nov.(♂♀, China:Guangxi);Oonopidae Simon, 1890: Orchestina xiebao Lin & Li, sp. nov.(♀, China:Chongqing);Philodromidae Thorell, 1869: Apollophanes lujiani Lin & Li, sp. nov.(♂♀, China:Guangxi) and Psellonus dawanqu Lin & Li, sp. nov.(♂♀, China: Guangdong);Salticidae Blackwall, 1841: Coccorchestes spark Lin & Li, sp. nov.(♂♀, Indonesia, West Papua),Onomastus zhuwu

关键词： Diagnosis new combination new genus new species Asia.

Comparative transcriptome analysis between rhesus macaques(Macaca mulatta) and crab-eating macaques(M. fascicularis)

在线全文

学校读者我要写书评

暂无评论

zoological Research 2024年第2期45卷 299-310页

作者： Yu-Xiang Mao Yamei Li Zikun Yang Ning Xu Shilong Zhang Xuankai Wang Xiangyu Yang Qiang Sun Yafei Mao Institute of Neuroscience State Key Laboratory of NeuroscienceCenter for Excellence in Brain Science&Intelligence TechnologyChinese Academy of SciencesShanghai 200031China Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology Shanghai 201210China University of Chinese Academy of Sciences Beijing 100049China key laboratory of Genetic evolution&Animal Models Chinese Academy of SciencesKunmingYunnan 650201China Bio-X Institutes Key Laboratory for the Genetics of Developmental and Neuropsychiatric DisordersMinistry of EducationShanghai Jiao Tong UniversityShanghai 200030China Zhiyuan College Shanghai Jiao Tong UniversityShanghai 200240China Center for Genomic Research International Institutes of MedicineFourth Affiliated HospitalZhejiang UniversityYiwuZhejiang 322000China

Understanding gene expression variations between species is pivotal for deciphering the evolutionary diversity in phenotypes. Rhesus macaques(Macaca mulatta, MMU)and crab-eating macaques(M. fascicularis, MFA) serve as crucial nonhuman primate biomedical models with different phenotypes. To date, however, large-scale comparative transcriptome research between these two species has not yet been fully explored. Here, we conducted systematic comparisons utilizing newly sequenced RNA-seq data from84 samples(41 MFA samples and 43 MMU samples)encompassing 14 common tissues. Our findings revealed a small fraction of genes(3.7%) with differential expression between the two species, as well as 36.5% of genes with tissue-specific expression in both macaques. Comparison of gene expression between macaques and humans indicated that 22.6% of orthologous genes displayed differential expression in at least two tissues. Moreover,19.41% of genes that overlapped with macaque-specific structural variants showed differential expression between humans and macaques. Of these, the FAM220A gene exhibited elevated expression in humans compared to macaques due to lineage-specific duplication. In summary,this study presents a large-scale transcriptomic comparison between MMU and MFA and between macaques and humans. The discovery of gene expression variations not only enhances the biomedical utility of macaque models but also contributes to the wider field of primate genomics.

关键词： Crab-eating macaques Rhesus macaques Comparative transcriptomics Biomedical models Nonhuman primates RNA-seq Duplicated genes