Comparative transcriptome analysis between rhesus macaques(Macaca mulatta) and crab-eating macaques(M. fascicularis)

作     者：Yu-Xiang Mao Yamei Li Zikun Yang Ning Xu Shilong Zhang Xuankai Wang Xiangyu Yang Qiang Sun Yafei Mao Yu-Xiang Mao;Yamei Li;Zikun Yang;Ning Xu;Shilong Zhang;Xuankai Wang;Xiangyu Yang;Qiang Sun;Yafei Mao

作者机构：Institute of NeuroscienceState Key Laboratory of NeuroscienceCenter for Excellence in Brain Science&Intelligence TechnologyChinese Academy of SciencesShanghai 200031China Shanghai Center for Brain Science and Brain-Inspired Intelligence TechnologyShanghai 201210China University of Chinese Academy of SciencesBeijing 100049China Key Laboratory of Genetic Evolution&Animal ModelsChinese Academy of SciencesKunmingYunnan 650201China Bio-X InstitutesKey Laboratory for the Genetics of Developmental and Neuropsychiatric DisordersMinistry of EducationShanghai Jiao Tong UniversityShanghai 200030China Zhiyuan CollegeShanghai Jiao Tong UniversityShanghai 200240China Center for Genomic ResearchInternational Institutes of MedicineFourth Affiliated HospitalZhejiang UniversityYiwuZhejiang 322000China 

出 版 物：《Zoological Research》 (动物学研究（英文）)

年 卷 期：2024年第45卷第2期

页      面：299-310页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071002[理学-动物学] 

基　　金：supported by the National Natural Science Foundation of China (82021001 and 31825018 to Q.S., 32370658 to Y.M.,82001372 to X.Y.) National Key Research and Development Program of China (2022YFF0710901) National Science and Technology Innovation2030 Major Program (2021ZD0200900) to Q.S Shanghai Pujiang Program (22PJ1407300) Shanghai Jiao Tong University 2030 Initiative (WH510363001-7) to Y.M 

主　　题：Crab-eating macaques Rhesus macaques Comparative transcriptomics Biomedical models Nonhuman primates RNA-seq Duplicated genes 

摘      要：Understanding gene expression variations between species is pivotal for deciphering the evolutionary diversity in phenotypes. Rhesus macaques(Macaca mulatta, MMU)and crab-eating macaques(M. fascicularis, MFA) serve as crucial nonhuman primate biomedical models with different phenotypes. To date, however, large-scale comparative transcriptome research between these two species has not yet been fully explored. Here, we conducted systematic comparisons utilizing newly sequenced RNA-seq data from84 samples(41 MFA samples and 43 MMU samples)encompassing 14 common tissues. Our findings revealed a small fraction of genes(3.7%) with differential expression between the two species, as well as 36.5% of genes with tissue-specific expression in both macaques. Comparison of gene expression between macaques and humans indicated that 22.6% of orthologous genes displayed differential expression in at least two tissues. Moreover,19.41% of genes that overlapped with macaque-specific structural variants showed differential expression between humans and macaques. Of these, the FAM220A gene exhibited elevated expression in humans compared to macaques due to lineage-specific duplication. In summary,this study presents a large-scale transcriptomic comparison between MMU and MFA and between macaques and humans. The discovery of gene expression variations not only enhances the biomedical utility of macaque models but also contributes to the wider field of primate genomics.