检索结果-南通市图书馆

evaluation of the basic functions of six calcium-dependent protein kinases in Toxoplasma gondii using CRISPR-Cas9 system

在线全文

学校读者我要写书评

暂无评论

Evaluation of the basic functions of six calcium-dependent p...

Profilings of serum exosomes identify diagnostic markers for echinococcosis

中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会

作者： Siyang Huang Jinlei Wang Tingting Li Kai Chen Hongrui Ning Xingquan Zhu State key laboratory of veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences

Toxoplasma gondii, an important protozoan parasite, infects almost all warm blooded animals and humans. Although treatments in T. gondii are limited by the lack of effective drugs, some calcium-dependent kinases were demonstrated as the promising drugs targets to chemotherapy against T. gondii due to their essential roles in T. gondii and absence from their hosts. The objectives of the present study were to investigate the functions of six calcium-dependent protein kinases(CDPK4, CDPK4 A, CDPK5, CDPK6, CDPK8, and CDPK9) in T. gondii to assess whether they are suitable for designing as drugs *** used the CRISPR-Cas9 system to disrupt six CDPK genessuccessfully by insertion of DHFR* at the guide RNA targeted region in the six endogenous CDPK loci, andsuccessfully obtained the six KO-CDPK strains. The biological characteristic of the six strains were evaluated by plaque assays, invasion, egress, replication and virulence assays, respectively. The results indicated that there was no significant difference between the six KO-CDPK strains and wild-type strain in virulence and the lytic cycle including invasion, egress, and replication. The conclusion was the six CDPKs are not essential for T. gondii lytic cycle and also not virulence factors for mice, suggesting the six CDPKsmay participate in other functions in T. gondii.

关键词： Toxoplasma gondii Calcium-dependent protein kinases(CDPKs) CRISPR-Cas9 system Gene functions

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Profilings of serum exosomes identify diagnostic markers for...

Immunogenicity of multi-epitope-based vaccine candidates administered with the adjuvant Gp96 against rabies

2021中国畜牧兽医学会兽医寄生虫学分会第二届青年科学家学术论坛

作者： Shanling Cao Junmei Zhang Rui Li Yadong Zheng Xiaola Guo State key laboratory of veterinary Etiological Biology key laboratory of veterinary Parasitology of Gansu Province Lanzhou veterinary research Institute Chinese Academy of Agricultural Sciences

Echinococcosis is a life-threatening disease in human caused by the metacestodes of Echinococcus *** disease has a long latent period up to 5-10 years and is largely underdiagnosed,partially due to the lack of effective early diagnostic *** liquid chromatography-mass spectrometry,we comparatively profiled the serum-derived exosomes of ***-infected mice and identified two parasite-origin proteins,thioredoxin peroxidase 1(TPx-1) and transitional endoplasmic reticulum ATPase(TER ATPase),being continuously released by parasites into the sera during the infection via *** ELISA,both TPx-1 and TER ATPase were shown to have a better performance in diagnosis of animal echinococcosis as early as 10 days post infection and of human echinococcosis compared with the ***,TER ATPase was further demonstrated to be suitable for evaluation of the prognosis of echinococcosis patients,with86.5% sensitivity and 91.7% *** study reveals the potential of TER ATPase and TPx-1 as novel promising early diagnostic and prognostic candidates for echinococcosis.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Virologica Sinica 2016年第2期31卷 168-175页

作者： Yange Niu Ye Liu Limin Yang Hongren Qu Jingyi Zhao Rongliang Hu Jing Li Wenjun Liu key laboratory of Pathogenic Microbiology and Immunology Institute of Microbiology ChineseAcademy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China laboratory of Epidemiology and key laboratory of Jilin Province for Zoonosis Prevention and Control Veterinary Research Institute Academy of Military Medical Sciences Changchun 130122 China Beijing Center for Animal Disease Control and Prevention Chinese veterinary Medical Association of Animal Clinic Branch Beijing 102629 China State key laboratory of veterinary Biotechnology Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin 150001 China

Rabies, a zoonotic disease, causes > 55,000 human deaths globally and results in at least 500 million dollars in losses every year. The currently available rabies vaccines are mainly inactivated and attenuated vaccines, which have been linked with clinical diseases in animals. Thus, a rabies vaccine with high safety and efficacy is urgently needed. Peptide vaccines are known for their low cost, simple production procedures and high safety. Therefore, in this study, we examined the efficacy of multi-epitope-based vaccine candidates against rabies virus. The ability of various peptides to induce epitope-specific responses was examined, and the two peptides that possessed the highest antigenicity and conservation, i.e., AR16 and h PAB, were coated with adjuvant canineGp96 and used to prepare vaccines. The peptides were prepared as an emulsion of oil in water(O/W) to create three batches of bivalent vaccine products. The vaccine candidates possessed high safety. Virus neutralizing antibodies were detected on the day 14 after the first immunization in mice and beagles, reaching 5–6 IU/m L in mice and 7–9 IU/m L in beagles by day 28. The protective efficacy of the vaccine candidates was about 70%–80% in mice challenged by a virulent strain of rabies virus. Thus, a novel multi-epitope-based rabies vaccine with Gp96 as an adjuvant was developed and validated in mice and dogs. Our results suggest that synthetic peptides hold promise for the development of novel vaccines against rabies.

关键词： rabies virus multi-epitope-based vaccine immunogenicity evaluation Gp96

B Cell Epitopes within VP1 of Type O Foot-and-mouth Disease Virus for Detection of Viral Antibodies

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2010年第1期25卷 18-26页

作者： Shan-dian GAO Jun-zheng DU Hui-yun CHANG Guo-zheng CONG Jun-jun SHAO Tong LIN Shuai SONG Qing-ge XIE National Foot-and-mouth Disease Reference laboratory Key Laboratory of Animal Virology of Ministry of Agriculture State Key Laboratory of Veterinary Etiologieal Biology Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046 China

In this study, the coding region of type O FMDV capsid protein VP1 and a series of codon optimized DNA sequences coding for VP1 amino acid residues 141-160 （epitopel）, tandem repeat 200-213 （epitope2 （＋2）） and the combination of two epitopes （epitopel-2） was genetically cloned into the prokaryotic expression vector PPRoExHTb and pGEX4T-1, respectively. VP1 and the fused epitopes GST-E1, GST-E2 （＋2） and GST-E1-2 were successfully solubly expressed in the cytoplasm of Escherichia coli and Western blot analysis demonstrated they retained antigenicity. Indirect VP1-ELISA and epitope ELISAs were subsequently developed to screen a panel of 80 field pig sera using LPB-ELISA as a standard test. For VP1-ELISA and all the epitope ELISAs, there were clear distinctions between the FMDV-positive and the FMDV-negative samples. Cross-reactions with pig sera positive to the viruses of swine vesicular disease virus that produce clinically indistinguishable syndromes in pigs or guinea pig antisera to FMDV strains of type A, C and Asia1 did not occur. The relative sensitivity and specificity for the GST-E1 ELISA, GST-E2 （＋2）, GST-E1-2 ELISA and VP1-ELISA in comparison with LPB-ELISA were 93.3% and 85.0%, 95.0% and 90%, 100% and 81.8%, 96.6% and 80.9% respectively. This study shows the potential use of the aforementioned epitopes as alternatives to the complex antigens used in current detection for antibody to FMDV structural proteins.

关键词： Foot-and-mouth disease virus ( FMDV) Serology Epitope ELISA

Effects of lysophosphatidic acid on human periodontal ligament stem cells from teeth extracted from dental patients

在线全文

学校读者我要写书评

暂无评论

The Journal of Biomedical research 2019年第2期33卷 122-130页

作者： Byung Cheol Kim Jae-In Song Kyoung-Ha So Sang-Hwan Hyun laboratory of veterinary Embryology and Biotechnology Veterinary Medical Center and College of Veterinary MedicineChungbuk National University Institute of Stem Cell & Regenerative Medicine Chungbuk National University

Despite their potential applications in future regenerative medicine, periodontal ligament stem cells(PDLSCs) are difficult to obtain in large amounts from patients. Therefore, maintaining sternness while expanding the cell numbers for medical use is the key to transitioning PDLSCs from the bench to the clinic. Lysophosphatidic acid(LPA), which is present in the human body and saliva, is a signaling molecule derived from phospholipids. In this study, we examined the effects of LPA on sternness maintenance in human PDLSCs. Several spindle-shaped and fibroblast-like periodontal ligament stem-like cell lines were established from PDLSC isolation. Among these cell lines, the most morphologically appropriate cell line was characterized. The expression levels of OCT4, NANOG(a stem cell marker), and CD90(a mesenchymal stem cell marker) were high. However, CD73(a negative marker of mesenchymal stem cells) expression was not observed. Notably, immunofluorescence analysis identified the expression of STRO-1, CD146(a mesenchymal stem cell marker), and sex determining region Y-box 2 at the protein level. In addition, lipid droplets were stained by Oil red O after the induction of adipogenesis for 21 days, and mineralized nodules were stained by Alizarin Red S after the induction of osteogenesis for 14 days. Alkaline phosphate staining also demonstrated the occurrence of osteogenesis. In summary, we established a human PDLSC line, which could be applied as a cell source for tissue regeneration in dental patients. However, further studies are needed to determine the detailed effects of LPA on PDLSCs.

关键词： periodontal ligament stem cell lysophosphatidic acid stemness primary cell culture

evaluation of energy values of high-fiber dietary ingredients with different solubility fed to growing pigs using the difference and regression methods

在线全文

学校读者我要写书评

暂无评论

Animal Nutrition 2021年第2期7卷 569-575页

作者： Zhengqun Liu Ruqing Zhong Kai Li Liang Chen Bifeng Zhang Lei Liu Hongfu Zhang State key laboratory of Animal Nutrition Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing100193China Institute of Animal Science and veterinary Tianjin Academy of Agricultural SciencesTianjin300381China

The objective of this study was to compare the energy values of high-fiber dietary ingredients with different solubility(sugar beet pulp[SBP]and defatted rice bran[DFRB])in growing pigs using the difference and the regression methods.A total of 21 barrows(initial BW,40.5±1.2 kg)were assigned to 3 blocks with BW as a blocking factor,and each block was assigned to a 7×2 incomplete Latin square design with 7 diets and two 13-d experimental *** 7 experimental diets consisted of a corn-soybean meal basal diet and 6 additional diets containing 10%,20%,or 30%SBP or DFRB in the basal diet,*** of the exper-imental periods lasted 12 d,with a 7 d dietary adaptation period followed by 5-d total fecal and urine *** showed that the digestible energy(DE)and metabolizable energy(ME)of the SBP determined by the difference method with different inclusion levels(10%,20%,or 30%)were 2,712 and 2,628 kcal/kg,2,683 and 2,580 kcal/kg,and 2,643 and 2,554 kcal/kg DM basis,*** DE and ME in the DFRB evaluated by the difference method with 3 different inclusion levels were 2,407 and 2,243 kcal/kg,2,687 and 2,598 kcal/kg,and 2,630 and 2,544 kcal/kg DM basis,*** inclusion levels had no effects on the energy values of each test ingredient estimated by the difference *** DE and ME of the SBP and the DFRB estimated by the regression method were 2,562 and 2,472 kcal/kg and 2,685 and 2,606 kcal/kg DM basis,*** energy values of each ingredient determined by the regression method were similar to the values estimated by the difference method with the 20%or 30%inclusion ***,the energy values of the SBP and DFRB estimated by the difference method with the 10%inclusion level were inconsistent with the values determined by the regression method(P<0.05).In conclusion,the regression method was a robust indirect method to evaluate the energy values for high-fiber ingredients with different solubility in growing pi

关键词： High-fiber ingredient Energy value Difference method Regression method Inclusion level Growing pigs

Immune Potential of a Novel Multiple-epitope Vaccine to FMDV Type Asia 1 in Guinea Pigs and Sheep

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2011年第3期26卷 190-197页

作者： Jun-jun Shao Jing-feng Wang Hui-yun Chang Ji-xing Liu College of veterinary Medicine Gansu Agricultural University Lanzhou 730070 China＂ State key laboratory of veterinary Etiological Biology Key Laboratory of Animal Virology of Ministry of AgricultureLanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046 China）

To develop a safe and efficient recombinant subunit vaccine to foot-and-mouth disease virus (FMDV) type Asia 1 in sheep, a tandem repeated multiple-epitope gene consisting of residues 137-160 and 197-211 of the VP1 gene of FMDV was designed and artificially synthesized. The biologically functional molecule, the ovine IgG heavy constant region (oIgG) as a protein carrier was introduced for design of the multiple-epitope recombinant vaccine and recombinant expression plasmids pET-30a-RE and pET-30a-RE-oIgG were successfully constructed. The recombinant proteins, RE and RE-oIgG, were expressed as a formation of inclusion bodies in E. coli. The immune potential of this vaccine regime in guinea pigs and sheep was evaluated. The results showed that IgG could significantly enhance the immune potential of antigenic epitopes. The recombinant protein RE-oIgG could not only elicit the high levels of neutralizing antibodies and lymphocytes proliferation responses in the vaccinated guinea pigs, but confer complete protection in guinea pigs against virus challenge. Although the recombinant protein RE could not confer protection in the vaccinated animals, it could delay the appearance of the clinical signs and reduce the severity of disease. Inspiringly, the titers of anti-FMDV neutralizing antibodies elicited in sheep vaccinated with RE-oIgG was significantly higher than that for the RE vaccination. Therefore, we speculated that this vaccine formulation may be a promising strategy for designing a novel vaccine against FMDV in the future.

关键词： Vaccine Foot-and-mouth disease Guinea pigs Epitope

Efficacy evaluation of the C-strain-based vaccines against the subgenotype 2.1d classical swine fever virus emerging in China

在线全文

学校读者我要写书评

暂无评论

Efficacy evaluation of the C-strain-based vaccines against t...

Long-term,multidomain analyses to identify the breed and allelic effects in MSTN-edited pigs to overcome lameness and sustainably improve nutritional meat production

中国畜牧兽医学会动物传染病学分会第九次全国会员代表大会暨第十七次全国学术研讨会

作者：罗玉子 Shengwei Ji Jian-Lin Lei Guang-Tao Xiang Xing-Yu Meng Guanglai Zheng Yimin Wang Yongfeng Li Su Li Xi-Jun He Yuan Sun Hua-Ji Qiu State key laboratory of veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural Sciences

Classical swine fever(CSF) is a devastating infectious disease of pigs caused by classical swi ne fever virus(CSFV).The disease has been controlled following extensive vaccination with the 1 apinized attenuated vaccine C-strain for decades in ***,frequent CSF outbreaks occurr ed recently in a large number of C-strain-vaccinated pig farms in China and a new subgenotype 2.1 d of CSFV has been reported to be responsible for the *** we analyzed the molecul ar variations and antigenic differences among the C-strain-based commercial vaccines of different o rigins from different manufacturers in China,and reevaluated the vaccines against the emerging su bgenotype 2.1 d strain of *** results showed that the C-strain adapted to the continuous ST cell line(C) contain a unique M290 K variation on the E2 protein,compared to those of prima ry BT cells(C) or rabbit origin(C) and the traditional C-strain sequences available in the Ge nBank *** neutralization test revealed the antigenic differences between Cst and Cbt or ***,the neutralizing titers of porcine anti-C-strain sera against the CSFV isolate of su bgenotype 2.1 d were significantly lower than those against C-strain or Shimen *** C-strainvaccinated,subgenotype 2.1 d HLJZZ2014 strain-challenged pigs did not show any clinical signs an d all ***,these pigs displayed mild pathological and histological lesions,and the C SFV viral RNA was detected in the various tissue and blood *** together,the C-strain-based vaccines of different origins showed molecular variations and antigenic differences,and cou1 d provide clinical but not pathological and virological protection against the subgenotype 2.1 d CS FV emerging in *** investigation is needed to comprehensively assess the efficacy of C-strain of different doses against the subgenotype 2.1 d CSFV.

关键词： Classical swine fever virus Subgenotype 2.1d C-strain vaccines Efficacy

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2022年第2期65卷 362-375页

作者： Ziyao Fan Zhiguo Liu Kui Xu Tianwen Wu Jinxue Ruan Xinmin Zheng Shideng Bao Yulian Mu Tad Sonstegard Kui Li State key laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China Hubei key laboratory of Animal Embryo Engineering and Molecular Breeding Hubei Institute of Animal Science and Veterinary MedicineHubei Academy of Agricultural SciencesWuhan 430064China Department of Stem Cell Biology and Regenerative Medicine Lerner Research InstituteCleveland ClinicCleveland OH 44195USA Acceligen Inc. Eagan MN 55121USA

Beef and mutton production has been aided by breeding to integrate allelic diversity for myostatin(MSTN),but a lack of diversity in the MSTN germplasm has limited similar advances in pig ***,insurmountable challenges with congenital lameness and a dearth of data about the impacts of feed conversion,reproduction,and meat quality in MSTN-edited pigs have also currently blocked ***,in a largest-to-date evaluation of multiple MSTN-edited pig populations,we demonstrated a practical alternative edit-site-based solution that overcomes the major production obstacle of hindlimb *** also provide long-term and multidomain datasets for multiple breeds that illustrate how MSTN-editing can sustainably increase the yields of breed-specific lean meat and the levels of desirable lipids without deleteriously affecting feed-conversion rates or litter *** from establishing a new benchmark for the data scale and quality of genome-edited animal production,our study specifically illustrates how gene-editing site selection profoundly impacts the phenotypic outcomes in diverse genetic back-grounds.

关键词： MSTN-edited pigs multidomain evaluation genetic background editing strategy