Long-term,multidomain analyses to identify the breed and allelic effects in MSTN-edited pigs to overcome lameness and sustainably improve nutritional meat production

作     者：Ziyao Fan Zhiguo Liu Kui Xu Tianwen Wu Jinxue Ruan Xinmin Zheng Shideng Bao Yulian Mu Tad Sonstegard Kui Li Ziyao Fan;Zhiguo Liu;Kui Xu;Tianwen Wu;Jinxue Ruan;Xinmin Zheng;Shideng Bao;Yulian Mu;Tad Sonstegard;Kui Li

作者机构：State Key Laboratory of Animal NutritionInstitute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193China Hubei Key Laboratory of Animal Embryo Engineering and Molecular BreedingHubei Institute of Animal Science and Veterinary MedicineHubei Academy of Agricultural SciencesWuhan 430064China Department of Stem Cell Biology and Regenerative MedicineLerner Research InstituteCleveland ClinicCleveland OH 44195USA Acceligen Inc.Eagan MN 55121USA 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2022年第65卷第2期

页      面：362-375页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0905[农学-畜牧学] 09[农学] 090501[农学-动物遗传育种与繁殖] 

基　　金：the National Major Transgenic Breeding Project(2008ZX08006-003,2011ZX08006-003,2013ZX08006-003,2014ZX08006-003,and 2016ZX08006-001) the Na-tional Key Basic Research Development Plan(2015CB943100) the Key Projects of the National Natural Science Foundation of China(30830080 and 31330074) China Postdoctoral Foundation Project(2018M631648) 

主　　题：MSTN-edited pigs multidomain evaluation genetic background editing strategy 

摘      要：Beef and mutton production has been aided by breeding to integrate allelic diversity for myostatin(MSTN),but a lack of diversity in the MSTN germplasm has limited similar advances in pig ***,insurmountable challenges with congenital lameness and a dearth of data about the impacts of feed conversion,reproduction,and meat quality in MSTN-edited pigs have also currently blocked ***,in a largest-to-date evaluation of multiple MSTN-edited pig populations,we demonstrated a practical alternative edit-site-based solution that overcomes the major production obstacle of hindlimb *** also provide long-term and multidomain datasets for multiple breeds that illustrate how MSTN-editing can sustainably increase the yields of breed-specific lean meat and the levels of desirable lipids without deleteriously affecting feed-conversion rates or litter *** from establishing a new benchmark for the data scale and quality of genome-edited animal production,our study specifically illustrates how gene-editing site selection profoundly impacts the phenotypic outcomes in diverse genetic back-grounds.