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Qian-Zheng-San promotes regeneration after sciatic nerve crush injury in rats

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Neural regeneration Research 2019年第4期14卷 683-691页

作者： Zhi-Yong Wang Li-Hua Qin Wei-Guang Zhang Pei-Xun Zhang Bao-Guo Jiang Department of Anatomy and Histo-embryology School of Basic Medical Sciences Peking University Department of trauma and Orthopedics Peking University People's Hospital

Qian-Zheng-San, a traditional Chinese prescription consisting of Typhonii Rhizoma, Bombyx Batryticatus, Scorpio, has been found to play an active therapeutic role in central nervous system diseases. However, it is unclear whether Qian-Zheng-San has therapeutic value for peripheral nerve injury. Therefore, we used Sprague-Dawley rats to investigate this. A sciatic nerve crush injury model was induced by clamping the right sciatic nerve. Subsequently, rats in the treatment group were administered 2 mL Qian-Zheng-San(1.75 g/mL) daily as systemic therapy for 1, 2, 4, or 8 weeks. Rats in the control group were not administered Qian-Zheng-San. Rats in sham group did not undergo surgery and systemic therapy. Footprint analysis was used to assess nerve motor function. Electrophysiological experiments were used to detect nerve conduction function. Immunofluorescence staining was used to assess axon counts and morphological analysis. Immunohistochemical staining was used to observe myelin regeneration of the sciatic nerve and the number of motoneurons in the anterior horn of the spinal cord. At 2 and 4 weeks postoperatively, the sciatic nerve function index, nerve conduction velocity, the number of distant regenerated axons and the axon diameter of the sciatic nerve increased in the Qian-Zheng-San treatment group compared with the control group. At 2 weeks postoperatively, nerve fiber diameter, myelin thickness, and the number of motor neurons in the lumbar spinal cord anterior horn increased in the Qian-Zheng-San treatment group compared with the control group. These results indicate that QianZheng-San has a positive effect on peripheral nerve regeneration.

关键词： nerve regeneration traditional Chinese medicine crush injury peripheral nerve regeneration nerve conduction velocity sciatic function index nerve injury nerve repair formula scorpion neural regeneration

Role of inflammation in neurological damage and regeneration following spinal cord injury and its therapeutic implications

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Burns & trauma 2023年第1期11卷 62-71页

作者： Yan Jin Yixing Song Jiaqi Lin Tianqing Liu Guicai Li Biqin Lai Yun Gu Gang Chen Lingyan Xing key laboratory of neuroregeneration of Jiangsu and the Ministry of Education Co-innovation Center of Neurore-generationNMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology ProductsNantong UniversityNantong 226006China School of Life Sciences Nantong UniversityNantong 226019China School of Medicine Nantong UniversityNantong 226006China NICM Health Research Institute Western Sydney UniversityWestmeadNSW 2145Australia key laboratory for Stem Cells and Tissue Engineering(Sun Yat-sen University) Ministry of EducationGuangzhou 510275China Co-innovation Center of neuroregeneration Nantong Univer-sityNantong 226006China

Spinal cord injury(SCI)is an incurable trauma that frequently results in partial or complete loss of motor and sensory *** neurons are damaged after the initial mechanical *** injuries,which are triggered by immunological and inflammatory responses,also result in neuronal loss and axon *** results in defects in the neural circuit and a deficiency in the processing of *** inflammatory responses are necessary for spinal cord recovery,conflicting evidence of their contributions to specific biological processes have made it difficult to define the specific role of inflammation in *** review summarizes our understanding of the complex role of inflammation in neural circuit events following SCI,such as cell death,axon regeneration and neural *** also review the drugs that regulate immune responses and inflammation in the treatment of SCI and discuss the roles of these drugs in the modulation of neural ***,we provide evidence about the critical role of inflammation in facilitating spinal cord neural circuit regeneration in zebrafish,an animal model with robust regenerative capacity,to provide insights into the regeneration of the mammalian central nervous system.

关键词： Inflammation Immune response Spinal cord injury Axon regeneration Cell death Zebrafish Inflammatory drugs trauma neurological damage

Effects of increased human tumor necrosis factor-like molecule 1A expression in peripheral blood of children with acute Guillain-Barre syndrome on interferon-gamma secretion

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Neural regeneration Research 2009年第10期4卷 763-768页

作者： Libin Yang Shulei Li Yan Tan Shufen Xu Xiumei Duan Yanqiu Fang Lihua Liu Yuanyuan Che Lei Liu Liwei Zhou Department of Pediatrics First Hospital Jilin University Changchun 130021 Jilin Province China Department of Histology and Embryology Bethune Medical School Jilin University Changchun 130021 Jilin Province China central laboratory First Hospital Jilin University Changchun 130021 Jilin Province China

BACKGROUND： Human tumor necrosis factor-like molecule 1A （hTL1A） is a strong T helper cell type 1 （Thl） co-stimulator. Guillain-Barre syndrome （GBS） is an autoimmune disorder of the nervous system, which is mediated by Thl cells. OBJECTIVE： To determine hTL1A expression in peripheral blood T lymphocytes of acute GBS children and the effects of hTL1A on secretion of interferon-γ. DESIGN, TIME AND SETTING： A randomized, controlled, neuroimmunological in vitro study was performed at the central laboratory of First Hospital of Jilin University, China from November 2005 to November 2007. MATERIALS： Venous blood samples were obtained from 6 healthy donors, aged 6-12 years （all routine blood examination items were normal）, and 6 additional children with acute GBS, aged 6-12 years. The GBS children fell ill within 1 week and were not treated with hormones or immunoglobulin Purified recombinant human soluble tumor necrosis factor-like molecule 1A （rhsTL1A, 1 mg/mL, relative molecular mass 22 000, 6× His tag, soluble form） was supplied by the central laboratory of First Hospital of Jilin University, China. METHODS： Peripheral blood mononuclear cells were isolated from healthy donors using the standard Ficoll gradient centrifugation and were incubated in 96-well culture plates. The cells were assigned to the following groups： control （2 μg/mL phytohemagglutinin）, 2μg/mL phytohemagglutinin ＋ 25, 100 and 400 ng/mL rhsTL1A. T cell proliferation was quantified using the tritiated thymidine （3H-TdR） method. Serum interferon-γ levels in acute GBS children were detected by enzyme-linked immunosorbent assay （ELISA）. The ratio of hTL1A-positive T cells to CD3-positive T cells in peripheral blood of acute GBS children was determined using flow cytometry. Following in vitro pre-activation of peripheral blood mononuclear cells by 2 μg/mL phytohemagglutinin, the peripheral blood mononuclear cells were treated with 400 ng/mL exogenous rhsTLIA. Finally, peripheral blood mononuclear cell-secr

关键词： human tumor necrosis factor-like molecule 1 A cell proliferation Guillain-Barre syndrome interferon-γ

Liproxstatin-1 is an effective inhibitor of oligodendrocyte ferroptosis induced by inhibition of glutathione peroxidase 4

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Neural regeneration Research 2021年第3期16卷 561-566页

作者： Bao-You Fan Yi-Lin Pang Wen-Xiang Li Chen-Xi Zhao Yan Zhang Xu Wang Guang-Zhi Ning Xiao-Hong Kong Chang Liu Xue Yao Shi-Qing Feng International Cooperation Base of Spinal Cord Injury Tianjin Key Laboratory of Spine and Spinal CordDepartment of OrthopedicsTianjin Medical University General HospitalTianjinChina School of Medicine Nankai UniversityTianjinChina

Our previous studies showed that ferroptosis plays an important role in the acute and subacute stages of spinal cord *** intracellular iron levels and low glutathione levels make oligodendrocytes vulnerable to cell death after central nervous system *** this study,we established an oligodendrocyte(OLN-93 cell line)model of ferroptosis induced by RSL-3,an inhibitor of glutathione peroxidase 4(GPX4).RSL-3 significantly increased intracellular concentrations of reactive oxygen species and ***-3 also inhibited the main antiferroptosis pathway,i.e.,SLC7A11/glutathione/glutathione peroxidase 4(xCT/GSH/GPX4),and downregulated acyl-coenzyme A synthetase long chain family member ***,we evaluated the ability of several compounds to rescue oligodendrocytes from ***-1 was more potent than edaravone or ***-1 not only inhibited mitochondrial lipid peroxidation,but also restored the expression of GSH,GPX4 and ferroptosis suppressor protein *** findings suggest that GPX4 inhibition induces ferroptosis in oligodendrocytes,and that liproxstatin-1 is a potent inhibitor of ***,liproxstatin-1 may be a promising drug for the treatment of central nervous system diseases.

关键词： cell death central nervous system factor ferroptosis oligodendrocyte oxidation pathway repair spinal cord injury

Self-assembling peptide nanofiber hydrogels for central nervous system regeneration

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Frontiers of Materials Science 2015年第1期9卷 1-13页

作者： Xi LID Bin PI Hui WANG Xiu-Mei WANG National Engineering laboratory for Modern Silk College of Textile and Clothing Engineering Soochow University Suzhou 215123 China Institute for Regenerative Medicine and Biomimetic Materials Key Laboratory of Advanced Materials School of Materials Science and Engineering Tsinghua University Beijing 100084 China Department of Orthopedics The First Affiliated Hospital of Soochow University Suzhou 215006 China

central nervous system （CNS） presents a complex regeneration problem due to the inability of central neurons to regenerate correct axonal and dendritic connections. However, recent advances in developmental neurobiology, cell signaling, cell-matrix interaction, and biomaterials technologies have forced a reconsideration of CNS regeneration potentials from the viewpoint of tissue engineering and regenerative medicine. The applications of a novel tissue regeneration-inducing biomaterial and stem cells are thought to be critical for the mission. The use of peptide nanoflber hydrogels in cell therapy and tissue engineering offers promising perspectives for CNS regeneration. Self-assembling peptide undergo a rapid transformation from liquid to gel upon addition of counterions or pH adjustment, directly integrating with the host tissue. The peptide nanofiber hydrogels have mechanical properties that closely match the native central nervous extracellular matrix, which could enhance axonal growth. Such materials can provide an optimal three dimensional microenvironment for encapsulated cells. These materials can also be tailored with bioactive motifs to modulate the wound environment and enhance regeneration. This review intends to detail the recent status of selfassembling peptide nanoflber hydrogels for CNS regeneration.

关键词： self-assembling peptide hydrogel central nervous system (CNS) nerveregeneration

Targeting acute inflammation to complement spinal cord repair

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Neural regeneration Research 2015年第10期10卷 1596-1598页

作者： Faith H.Brennan Marc J.Ruitenberg School of Biomedical Sciences The University of Queensland Queensland Brain Institute The University of Queensland trauma Critical Care and Recovery Brisbane Diamantina Health Partners

Immune effector mechanisms play key roles in the progressive（secondary）neurodegenerative changes that follow spinal cord injury（SCI）.In our recent paper（Brennan et al.,2015）,we showed that the inflammatory response to SCI includes rapid and robust activation of the innate immune complement system, with tissue levels of complement component 5a （C5a - an activation product generated by the proteolysis of complement factor 5 （C5）） peaking 12 to 24 hours post-iniurv.

关键词： SCI Targeting acute inflammation to complement spinal cord repair

Differential transcriptomic landscapes of multiple organs from SARS-CoV-2 early infected rhesus macaques

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Protein & Cell 2022年第12期13卷 920-939页

作者： Chun-Chun Gao Man Li Wei Deng Chun-Hui Ma Yu-Sheng Chen Yong-Qiao Sun Tingfu Du Qian-Lan Liu Wen-Jie Li Bing Zhang Lihong Sun Si-Meng Liu Fengli Li Feifei Qi Yajin Qu Xinyang Ge Jiangning Liu Peng Wang Yamei Niu Zhiyong Liang Yong-Liang Zhao Bo Huang Xiao-Zhong Peng Ying Yang Chuan Qin Wei-Min Tong Yun-Gui Yang CAS key laboratory of Genomic and Precision Medicine Collaborative Innovation Center of Genetics and DevelopmentCollege of Future TechnologyBeijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China China National Center for Bioinformation Beijing 100101China Sino-Danish College University of Chinese Academy of SciencesBeijing 101408China Department of Pathology Beijing Ditan HospitalCapital Medical UniversityBeijing 100000China Department of Pathology Institute of Basic Medical SciencesMolecular Pathology Research CenterChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China NHC key laboratory of Human Disease Comparative Medicine Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious DiseasesInstitute of Laboratory Animal ScienceChinese Academy of Medical Sciences and Comparative Medicine CenterPeking Union Medical CollegeBeijing 100021China Institute of Stem Cell and regeneration Chinese Academy of SciencesBeijing 100101China Institute of Medical Biology Chinese Academy of Medical Sciences and Peking Union Medical CollegeKunming 650031China Center for Experimental Animal Research Institute of Basic Medical Sciences Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China Department of Pathology Peking Union Medical College HospitalMolecular Pathology Research CenterChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China Department of Immunology&National key laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China Clinical Immunology Center Chinese Academy of Medical SciencesBeijing 100005China Department of Biochemistry&Molecular Biology Tongji Medical CollegeHuazhong University of Science&TechnologyWuhan 430030China State key laboratory of Medical Molecular Biology Department of Molecular Biology and BiochemistryInstitute of Basic Med

SARS-CoV-2 infection causes complicated clinical manifestations with variable multi-organ injuries,how-ever,the underlying mechanism,in particular immune responses in different organs,remains *** this study,comprehensive transcriptomic alterations of 14 tissues from rhesus macaque infected with SARS-CoV-2 were *** to normal controls,SARS-CoV-2 infection resulted in dysregulation of genes involving diverse functions in various examined tissues/organs,with drastic transcriptomic changes in cerebral cortex and right ***,cerebral cortex exhibited a hyperinflammatory state evidenced by sig-nificant upregulation of inflammation response-related ***,expressions of coagulation,angio-genesis and fibrosis factors were also up-regulated in cerebral *** on our findings,neuropilin 1(NRP1),a receptor of SARS-CoV-2,was significantly elevated in cerebral cortex post infection,accompanied by active immune response releasing inflammatory factors and signal transmission among tissues,which enhanced infection of the central nervous system(CNS)in a positive feedback way,leading to viral ***,our study depicts a multi-tissue/organ tran-scriptomic landscapes of rhesus macaque with early infection of SARS-CoV-2,and provides important insights into the mechanistic basis for COVID-19-asso-ciated clinical complications.

关键词： SARS-CoV-2 NRP1 inflammation central nervous system viral encephalitis rhesus macaque

Consensus by Chinese Expert Panel on Chlamydia trachomatis-Resistant and Chlamydia trachomatis-Persistent Infection

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Chinese Medical Journal 2017年第23期130卷 2852-2856页

作者： Man-Li Qi Yuan-Li Guo Qian-Qiu Wang Xiang-Sheng Chen Jian-De Han Xiao-Hong Su Wen-Hui Lun Hao Cheng Jin-Hua Xu Hong-Qing Tian Li Che Zhi-Yuan Ya Wen-Li Feng Juan Jiang Ping-Yu Zhou Xian-Biao Zo Hong-Hui Xu Wei-Min Shi Jun Liu Lin Zhu Quan-Zhong Liu Department of Dermatovenerology Tianjin Medical University General HospitalTianjin Neurological InstituteMinistry of Education and Tianjin City Key Laboratory of Post-neuroinjury Neuro-repair and Regeneration in Central Nervous SystemTianjin 300052China Department of Dermatovenerology Tianjin Union Medical CenterTianjin 300121China Department of Clinical Management National Center of STD ControlInstitute of DermatologyChinese Academy of Medical SciencesChina CDCNanjingJiangsu 210042China Department of Dermatovenerology The First Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong 510080China Institute of Dermatology Chinese Academy of Medical Sciences and Peking Union Medical CollegeNanjingJiangsu 210042China Department of Dermatovenerology Beijing Ditan Hospital Capital Medical UniversityBeijing 100015China Department of Dermatology Sir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang 310020China Department of Dermatology Huashan HospitalFudan UniversityShanghai 200040China Shandong Provincial Institute of Dermatology and Venereology Provincial Academy of Medical ScienceJinanShandong 250022China Department of Dermatovenerology The First Affiliated Hospital of Nanchang UniversityNanchangJiangxi 330006China Department of Infection Management and Disease Prevention China-Japan Friendship HospitalBeijing 100029China Department of Dermatovenerology The Second Hospital of Shanxi Medical UniversityTaiyuanShanxi 030001China STD Institute Shanghai Skin Disease HospitalShanghai 200050China Department of Dermatology The First Affiliated Hospital of PLA General HospitalBeijing 100048China Department of Dermatology The First Hospital of China Medical UniversityShenyangLiaoning 110001China Department of Dermatovenerology Shanghai General HospitalShanghai 200080China Department of Dermatovenerology The 4th People's Hospital of Qinghai ProvinceXiningQinghai 810014China Department of Dermatovenerology Shenzhen Hospital Southern Medical Universi

Chlamydia trachomatis （Ct） genital infection is the most common sexually transmitted disease （STD） in China and the US. The morbidity of Ct genital infection in China has increased from 32.48/100,000 in 2008 to 37.18/100,000 in 2015.[1] The major areas of Ct infections are concentrated in the Zhujiang Delta, Changjiang Delta, Minjiang Area, and West China. In these areas, the highest incidence of Ct infection reaches 615.99/100,000 citizens. In the US, there are 1,441,789 reported Ct, which include 627.2 females and 278.4 males per 100,000 population. It is now the most prevalent STD, with its rate increasing to 22% in males and 6% in females.[2] Ct genital infection can cause epididymitis, prostatitis, cervicitis, annexitis, infertility, and atopic pregnancy, which have been identified as the major public health problems.

关键词： Chlamydia trachomatis Persistent Infection Therapy Resistant

Lysine-specific demethylase 1 expression in zebrafish during the early stages of neuronal development

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Neural regeneration Research 2012年第34期7卷 2719-2726页

作者： Aihong Li Yong Sun Changming Dou Jixian Chen Jie Zhang Department of Internal neurology Affiliated Hospital of Nantong University key laboratory of Acupuncture Combined with Medication Nanjing University of Chinese Medicine Anhui Academy of Environmental Sciences Department of Cardiothoracic Surgery Third Affiliated Hospital of Wenzhou Medical College Department of Immunology School of Medicine Nantong University

Lysine-specific demethylase 1 （Lsdl） is associated with transcriptional coregulation via the modulation of histone methylation. The expression pattern and function of zebrafish Lsdl has not, however, been studied. Here, we describe the pattern of zebrafish Lsdl expression during different development stages. In the zebrafish embryo, Isdl mRNA was present during the early cleavage stage, indicating that maternally derived Lsdl protein is involved in embryonic patterning. During embryogenesis from 0 to 48 hours post-fertilization （hpf）, the expression of Isdl mRNA in the embryo was ubiquitous before 12 hpf and then became restricted to the antedor of the embryo （particularly in the brain） from 24 hpf to 72 hpf. Inhibition of Lsdl activity （by exposure to tranylcypromine） or knockdown of Isdl expression （by morpholino antisense oligonucleotide injection） led to the loss of cells in the brain and to a dramatic downregulatJon of neural genes, including gad65, gad75, and reelin, but not hey1. These findings indicate an important role of Lsdl during nervous system development in zebrafish.

关键词： zebrafish lysine-specific demethylase morpholino tranylcypromine nerve cells embryonicdevelopment histone methylation histone demethylase brain neural regeneration