Qian-Zheng-San promotes regeneration after sciatic nerve crush injury in rats

作     者：Zhi-Yong Wang Li-Hua Qin Wei-Guang Zhang Pei-Xun Zhang Bao-Guo Jiang 

作者机构：Department of Anatomy and Histo-embryology School of Basic Medical Sciences Peking University Department of Trauma and Orthopedics Peking University People's Hospital 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2019年第14卷第4期

页      面：683-691页

核心收录：

学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100104[医学-病理学与病理生理学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China,No.31571235(to PXZ),31771322(to PXZ),81401007(to ZYW) National Key Basic Research Program of China(973 Program),No.2014CB542201(to PXZ) Beijing Science and Technology New Star Cross Program of China,No.2018019(to PXZ) Natural Science Foundation of Beijing of China,No.7162098(to WGZ) Fostering Young Scholars of Peking University Health Science Center of China,No.BMU2017PY013(to PXZ) 

主　　题：nerve regeneration traditional Chinese medicine crush injury peripheral nerve regeneration nerve conduction velocity sciatic function index nerve injury nerve repair formula scorpion neural regeneration 

摘      要：Qian-Zheng-San, a traditional Chinese prescription consisting of Typhonii Rhizoma, Bombyx Batryticatus, Scorpio, has been found to play an active therapeutic role in central nervous system diseases. However, it is unclear whether Qian-Zheng-San has therapeutic value for peripheral nerve injury. Therefore, we used Sprague-Dawley rats to investigate this. A sciatic nerve crush injury model was induced by clamping the right sciatic nerve. Subsequently, rats in the treatment group were administered 2 mL Qian-Zheng-San(1.75 g/mL) daily as systemic therapy for 1, 2, 4, or 8 weeks. Rats in the control group were not administered Qian-Zheng-San. Rats in sham group did not undergo surgery and systemic therapy. Footprint analysis was used to assess nerve motor function. Electrophysiological experiments were used to detect nerve conduction function. Immunofluorescence staining was used to assess axon counts and morphological analysis. Immunohistochemical staining was used to observe myelin regeneration of the sciatic nerve and the number of motoneurons in the anterior horn of the spinal cord. At 2 and 4 weeks postoperatively, the sciatic nerve function index, nerve conduction velocity, the number of distant regenerated axons and the axon diameter of the sciatic nerve increased in the Qian-Zheng-San treatment group compared with the control group. At 2 weeks postoperatively, nerve fiber diameter, myelin thickness, and the number of motor neurons in the lumbar spinal cord anterior horn increased in the Qian-Zheng-San treatment group compared with the control group. These results indicate that QianZheng-San has a positive effect on peripheral nerve regeneration.