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Abiotic Stresses and Phytohormones Regulate Expression of FAD2 Gene in Arabidopsis thaliana

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Journal of Integrative Agriculture 2012年第1期11卷 62-72页

作者： YUAN Si-wei WU Xue-long LIU Zhi-hong LUO Hong-bing HUANG Rui-zhi key laboratory of plant metabolic engineering of zhejiang province/institute of virology and biotechnology Zhejiang Academy of Agricultural Sciences Hangzhou 310021 P.R.China College of Agronomy Hunan Agricultural University Changsha 410128 P.R. China

Modification of unsaturated fatty acid （FA） levels has been found to accompany multiple abiotic stress acclimations in many plants. Delta 12 fatty acid desaturase （FAD2） plays a critical role in the synthesis of polyunsaturated FAs in plant cells by converting oleic acid （18：1） to linoleic acid （18：2）. To better understand the relationship between polyunsaturated FAs metabolism and stress adaptation, the expression of FAD2 gene and changes in the FA compositions under various abiotic stresses and phytohormone treatments in Arabidopsis thaliana was investigated in this study. A 1 423-bp promoter of the FAD2 gene was cloned and characterized from Arabidopsis. Several putative hormone- and stress- inducible cis-elements were identified in the cloned promoter, which include salt- and pathogen-inducible GT-1 motifs, low-temperature-responsive MYC element, dehydration-responsive MYB element, and GA signaling related WRKY71OS element. To investigate the fine regulation of FAD2 gene, a recombinant FAD2 promoter-GUS construct was introduced into Arabidopsis plants. Histochemical study showed that the promoter was ubiquitously active and responsive not only to exogenous phytohormones including ABA, 24-eBL, and SA but also to darkness, temperature, salt, and sucrose stresses in Arabidopsis seedlings. Consistent with the expression change, treatments with exogenous 24-eBL, ABA, SA, and NaCl resulted in reduction in polyunsaturated FAs in Arabidopsis seedlings. These findings suggest that the FAD2 gene with a wide variety of putative response elements in its promoter is responsive to multiple phytohormones and abiotic stresses and therefore may play an important role in stress responses of Arabidopsis during plant growth and seed development.

关键词： FAD2 abiotic stresses phytohormones fatty acid (FA) Arabidopsis thaliana

WRKY33-mediated indolic glucosinolate metabolic pathway confers resistance against Alternaria brassicicola in Arabidopsis and Brassica crops

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Journal of Integrative plant Biology 2022年第5期64卷 1007-1019页

作者： Han Tao Huiying Miao Lili Chen Mengyu Wang Chuchu Xia Wei Zeng Bo Sun Fen Zhang Shuqun Zhang Chuanyou Li Qiaomei Wang Department of Horticulture Zhejiang UniversityHangzhou 310058ZhejiangChina State key laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro‐products Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang ProvinceInstitute of Virology and BiotechnologyZhejiang Academy of Agricultural SciencesHangzhou 310021China College of Horticulture Sichuan Agricultural UniversityChengdu 611130China Division of Biochemistry Interdisciplinary Plant GroupUniversity of MissouriColumbia 65211MissouriUSA State key laboratory of plant Genomics National Centre for Plant Gene Research(Beijing)Institute of Genetics and Developmental Biologythe Chinese Academy of SciencesBeijing 100101China

The tryptophan(Trp)-derived plant secondary metabolites,including camalexin,4-hydroxyindole-3-carbonylnitrile,and indolic glucosinolate(IGS),show broad-spectrum antifungal ***,the distinct regulations of these metabolic pathways among different plant species in response to fungus infection are rarely *** this study,our results revealed that WRKY33 directly regulates IGS biosynthesis,notably the production of 4-methoxyindole-3-ylmethyl glucosinolate(4MI3G),conferring resistance to Alternaria brassicicola,an important pathogen which causes black spot in Brassica ***33 directly activates the expression of CYP81F2,IGMT1,and IGMT2 to drive sidechain modification of indole-3-ylmethyl glucosinolate(I3G)to 4MI3G,in both Arabidopsis and Chinese kale(Brassica oleracea *** Bailey).However,Chinese kale showed a more severe symptom than Arabidopsis when infected by Alternaria *** analyses of the origin and evolution of Trp metabolism indicate that the loss of camalexin biosynthesis in Brassica crops during evolution might attenuate the resistance of crops to Alternaria *** a result,the IGS metabolic pathway mediated by WRKY33 becomes essential for Chinese kale to deter Alternaria *** results highlight the differential regulation of Trp-derived camalexin and IGS biosynthetic pathways in plant immunity between Arabidopsis and Brassica crops.

关键词： Alternaria brassicicola glucosinolate plant defense secondary metabolism WRKY33

Dynamic UAV Phenotyping for Rice Disease Resistance Analysis Based on Multisource Data

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plant Phenomics 2023年第1期5卷 110-122页

作者： Xiulin Bai Hui Fang Yong He Jinnuo Zhang Mingzhu Tao Qingguan Wu Guofeng Yang Yuzhen Wei Yu Tang Lie Tang Binggan Lou Shuiguang Deng Yong Yang Xuping Feng College of Biosystems engineering and Food Science Zhejiang UniversityHangzhou 310058China Huzhou institute of zhejiang University Huzhou 313000China School of Information engineering Huzhou UniversityHuzhou 313000China Academy of Interdisciplinary Studies Guangdong Polytechnic Normal UniversityGuangzhou 510665China Department of Agricultural and Biosystems engineering Iowa State UniversityAmesIA 50011-3270USA College of Agriculture and biotechnology Zhejiang UniversityHangzhou 310058China College of Computer Science and Technology Zhejiang UniversityHangzhou 310058China State key laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products Key Laboratory of Biotechnology for Plant ProtectionMinistry of Agricultureand Rural AffairsZhejiang Provincial Key Laboratory of Biotechnology for Plant ProtectionInstitute of Virology and BiotechnologyZhejiang Academy of Agricultural ScienceHangzhou 31002China

Bacterial blight poses a threat to rice production and food security,which can be controlled through large-scale breeding efforts toward resistant *** aerial vehicle(UAV)remote sensing provides an alternative means for the infield phenotype evaluation of crop disease resistance to relatively time-consuming and laborious traditional ***,the quality of data acquired by UAV can be affected by several factors such as weather,crop growth period,and geographical location,which can limit their utility for the detection of crop disease and resistant ***,a more effective use of UAV data for crop disease phenotype analysis is *** this paper,we used time series UAV remote sensing data together with accumulated temperature data to train the rice bacterial blight severity evaluation *** best results obtained with the predictive model showed an R_(p)^(2) of 0.86 with an RMSE_(p) of ***,model updating strategy was used to explore the scalability of the established model in different geographical *** percent of transferred data for model training was useful for the evaluation of disease severity over different *** addition,the method for phenotypic analysis of rice disease we built here was combined with quantitative trait loci(QTL)analysis to identify resistance QTL in genetic populations at different growth *** new QTLs were identified,and QTLs identified at different growth stages were *** analysis combined with UAV high-throughput phenotyping provides new ideas for accelerating disease resistance breeding.

关键词： blight breeding cultivar

Seed-Specific Overexpression of Bnrbc S from Oilseed Increases Seed Weight and Oil Content in Arabidopsis thaliana

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Seed-Specific Overexpression of Bnrbc S from Oilseed Increas...

HEMIPTERAN-TRANSMITTED plant VIRUSES: RESEARCH PROGRESS AND CONTROL STRATEGIES

2010全国植物生物学研讨会

作者： Xue-Long Wu Zhi-Hong Liu Si-Wei Yuan Rui-Zhi Huang key laboratory of plant metabolic engineering of zhejiang province Institute of Virology and Biotechnology Zhejiang Academy of Agricultural Sciences

Ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco),which catalyzes the first step of CO2fixation,is the key photosynthetic enzyme to convert atmospheric carbon to food and support life on the *** higher plants,R... 详细信息

关键词： BnrbcS Brassica napus gene seed weight oil content

来源： cnki会议评论

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cnki会议

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Frontiers of Agricultural Science and engineering 2022年第1期9卷 98-109页

作者： Haijian HUANG Junmin LI Chuanxi ZHANG Jianping CHEN State key laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Key Laboratory of Biotechnology in Plant Protection(Ministry of Agriculture and Rural Affairs of China and Zhejiang Province)Institute of Plant VirologyNingbo UniversityNingbo 315211China

About 80% of plant viruses are transmitted by specific insect vectors, especiallyhemipterans with piercing-sucking mouthparts. Many virus-transmitting insectsare also important crop pests that cause considerable losses in crop *** review summarizes the latest research findings on the interactions betweenplant viruses and insect vectors and analyzes the key factors affecting insecttransmission of plant viruses from the perspectives of insect immunity, insectfeeding, and insect symbiotic microorganisms. Additionally, by referring to thelatest applications for blocking the transmission of animal viruses, potentialcontrol strategies to prevent the transmission of insect-vectored plant virusesusing RNAi technology, gene editing technology, and CRISPR/Cas9 + gene-driventechnology are discussed.

关键词： control strategies feeding immunity insect vector microorganism plant virus

Identification of molecular markers linked to rice bacterial blight resistance genes from Oryza meyeriana

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Frontiers of Agricultural Science and engineering 2015年第3期2卷 260-265页

作者： Jing WANG Chen CHENG Yanru ZHOU Yong YANG Qiong MEI Junmin LI Ye CHENG Chengqi YAN Jianping CHEN College of Chemistry and Life Sciences Zhejiang Normal UniversityJinhua 321000China State key laboratory Breeding Base for Sustainable Control of plant Pest and Disease Key Laboratory of Biotechnology in Plant Protection of the Ministry of Agriculture and Zhejiang ProvinceInstitute of Virology and BiotechnologyZhejiang Academy of Agricultural SciencesHangzhou 310021China College of plant Protection Shenyang Agricultural UniversityShenyang 110866China

Y73 is a progeny of asymmetric somatic hybridization between Oryza sativa *** and the wild rice species Oryza *** with a range of strains of Xanthomonas oryzae *** showed that Y73 had inherited a high level of resistance to rice bacterial blight(BB)from its wild *** F2 population of 7125 individuals was constructed from the cross between Y73 and a BB-susceptible cultivar *** testing 615 SSR and STS markers covering the 12 rice chromosomes,186 markers were selected that showed polymorphism between Y73 and *** markers linked to the BB resistance genes in Y73 were scanned using the F2 population and the polymorphic *** SSR marker RM128 on chromosome 1,the STS marker R03D159 on chromosome 3 and the STS marker R05D104 on chromosome 5 were found to be linked to the rice BB resistance genes in Y73.

关键词： Oryza meyeriana bacterial blight resistance gene genetic population linkage molecular marker

Construction and Application of Efficient Ac-Ds Transposon Tagging Vectors in Rice

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Journal of Integrative plant Biology 2009年第11期51卷 982-992页

作者： Shaohong Qu Jong-Seong Jeont Pieter B.F. Ouwerkerk Maria Bellizzi Jan Leach Pamela Ronald Guo-Liang Wang Department of plant Pathology Ohio State University Columbus OH 43210 USA institute of virology and biotechnology Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China Department of plant Pathology University of California Davis CA 95616 USA Graduate School of biotechnology and plant Metabolism Research Center Yongin 446-701 Korea institute of Biology Leiden University Clusius Laboratory RA Leiden 2300 The Netherlands Bioagdcultural Sciences and Pest Management Colorado State University Fort Collins CO 80523 USA Crop Gene engineering key laboratory of Hunan province and Pre-State key laboratory of Crop Germplasm Renovation and Resource Utilization Hunan Agricultural University Changsha 410128 China

Transposons are effective mutagens alternative to T-DNA for the generation of insertional mutants in many plant species including those whose transformation is inefficient. The current strategies of transposon tagging are usually slow and labor-intensive and yield low frequency of tagged lines. We have constructed a series of transposon tagging vectors based on three approaches：（i） AcTPase controlled by glucocorticoid binding domain/VP16 acidic activation domain/Gal4 DNA-binding domain （GVG） chemical-inducible expression system; （ii） deletion of AcTPase via Cre-lox site-specific recombination that was initially triggered by Ds excision; and （iii） suppression of early transposition events in transformed rice callus through a dual-functional hygromycin resistance gene in a novel Ds element （HPT-Ds）, We tested these vectors in transgenic rice and characterized the transposition events. Our results showed that these vectors are useful resources for functional genomics of rice and other crop plants. The vectors are freely available for the community,

关键词： Ac-Ds transposable element glucocorticoid binding domain/VP16 acidic activation domain/Gal4 DNA-binding domain-inducible expression Cre-lox site-specific recombination rice.

Distribution of microplastics in soil aggregates after film mulching

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Soil Ecology Letters 2023年第3期5卷 37-51页

作者： Yuhuai Liu Yingying Zhong Can Hu Mouliang Xiao Fan Ding Yongxiang Yu Huaiying Yao Zhenke Zhu Jianping Chen Tida Ge Jina Ding State key laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Key Laboratory of Biotechnology in Plant Protectionof MARA and Zhejiang ProvinceInstitute of Plant VirologyNingbo UniversityNingbo 315211China Ningbo Customs Technology Center Ningbo 315012China College of Mechanical and Electrical engineering Tarim UniversityAlar 843300China College of Land and Environment Shenyang Agricultural UniversityShenyang 110866China Research Center for Environmental Ecology and engineering School of Environmental Ecology and Biological EngineeringWuhan Institute of TechnologyWuhan 430205China

Microplastic distribution is non-homogeneous in agricultural soil following plastic film ***,the distribution of microplastics by shape and particle size in different soil aggregates remains *** elucidate the distribution of microplastic shapes and particle sizes in soil aggregates with increasing years of film mulching,four paired fields with film mulching(FM)and no mulching(NM)were examined at 1,5,10,and 20 years after continuous *** increase in soil aggregates of 0.053–0.25 mm diameter was observed;however,soil organic carbon content decreased after long-term *** primarily combined with 0.053–2 mm soil ***,long-term FM was associated with dominance of film-and fiber-shaped microplastics in soil aggregates of 0.25–2 mm and 0.053–0.25 mm diameter,***-and granule-shaped microplastics of 0.25–1 mm diameter primarily combined with 0.053–0.25 and 0.25–2 mm soil aggregates,***-shaped microplastics of diameter>1 mm and diameter 0.05–0.25 mm primarily combined with 0.25–2 mm soil ***,distribution of microplastics in soil aggregates can be used to monitor soil health and quality,greatly enhancing our understanding of the risk posed by microplastics to the environment.

关键词： film mulching microplastic soil aggregate agricultural soil

New approaches for evaluation of soil health, sensitivity and resistance to degradation

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Frontiers of Agricultural Science and engineering 2020年第3期7卷 282-288页

作者： Yakov KUZYAKOV Anna GUNINA Kazem ZAMANIAN Jing TIAN Yu LUO Xingliang XU Anna YUDINA Humberto APONTE Hattan ALHARBI Lilit OVSEPYAN Irina KURGANOVA Tida GE Thomas GUILLAUME Department of Soil Science of Temperate Ecosystems Department of Agricultural Soil ScienceUniversity of Goettingen37077 GottingenGermany College of Food and Agriculture Sciences King Saud UniversityRiyadh 11451Saudi Arabia Department of Environmental Chemistry University of Kassel37213 WitzenhausenGermany College of Resources and Environmental Sciences National Academy of Agriculture Green DevelopmentKey Laboratory of Plant-Soil Interactions(Ministry of Education)China Agricultural UniversityBeijing 100193China institute of Soil and Water Resources and Environmental Science Zhejiang Provincial Key Laboratory of Agricultural Resources and EnvironmentZhejiang UniversityHangzhou 310058China institute of Geographic Sciences and Natural Resources Research Chinese Academy of SciencesBeijing 100101China CAS Center for Excellence in Tibetan Plateau Earth Sciences Chinese Academy of Sciences(CAS)Beijing 100101China Department of Soil Physics and Hydrology V.V.Dokuchaev Soil Science InstituteMoscow 119017Russia Center for Research in Mycorrhiza and Agri-Environmental Sustainability(CIMYSA) University of La FronteraTemucoChile institute of Physicochemical and Biological Problems in Soil Science Russian Academy of Sciences(RAS)Moscow 142290Russia State key laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture of China and Zhejiang ProvinceInstitute of Plant VirologyNingbo UniversityNingbo 315211China laboratory of Biogeosciences Institute of Earth Surface DynamicsUniversity of LausanneCH-1015 LausanneSwitzerland

Assessment of soil health requires complexevaluation of properties and functions responsible for abroad range of ecosystem services. Numerous soil qualityindices (SQI) have been suggested for the evaluation ofspecific groups of soil functions, but comparison of variousSQI is impossible because they are based on a combinationof specific soil properties. To avoid this problem, wesuggest an SQI-area approach based on the comparison ofthe areas on a radar diagram of a combination of chemical,biological and physical properties. The new approach isindependent of the SQI principle and allows rapid andsimple comparison of parameter groups and soils. Anotherapproach analyzing the resistance and sensitivity ofproperties to degradation is suggested for a detailedevaluation of soil health. The resistance and sensitivityof soil properties are determined through comparison withthe decrease of soil organic carbon (SOC) as a universalparameter responsible for many functions. The SQI-areaand resistance/sensitivity approaches were tested based on quences after the ab and on ment of agricultural soils. Both the SQI-area and the resistance/sensitivity approaches areuseful for basic and applied research, and for decisionmakersto evaluate land-use practices and measure thedegree of soil degradation.

关键词： ecosystem stability land quality land degradation soil health soil management soil organic matter