Abiotic Stresses and Phytohormones Regulate Expression of FAD2 Gene in Arabidopsis thaliana

作     者：YUAN Si-wei WU Xue-long LIU Zhi-hong LUO Hong-bing HUANG Rui-zhi 

作者机构：Key Laboratory of Plant Metabolic Engineering of Zhejiang Province/Institute of Virology and Biotechnology Zhejiang Academy of Agricultural Sciences Hangzhou 310021 P.R.China College of Agronomy Hunan Agricultural University Changsha 410128 P.R. China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2012年第11卷第1期

页      面：62-72页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0903[农学-农业资源与环境] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：supported by grants from the National HighTech R&D Program of China (2008AA02Z103) the National Natural Science Foundation of China (30671332) the Natural Science Foundation of Zhejiang Province,China (Z304430) 

主　　题：FAD2 abiotic stresses phytohormones fatty acid (FA) Arabidopsis thaliana 

摘      要：Modification of unsaturated fatty acid （FA） levels has been found to accompany multiple abiotic stress acclimations in many plants. Delta 12 fatty acid desaturase （FAD2） plays a critical role in the synthesis of polyunsaturated FAs in plant cells by converting oleic acid （18：1） to linoleic acid （18：2）. To better understand the relationship between polyunsaturated FAs metabolism and stress adaptation, the expression of FAD2 gene and changes in the FA compositions under various abiotic stresses and phytohormone treatments in Arabidopsis thaliana was investigated in this study. A 1 423-bp promoter of the FAD2 gene was cloned and characterized from Arabidopsis. Several putative hormone- and stress- inducible cis-elements were identified in the cloned promoter, which include salt- and pathogen-inducible GT-1 motifs, low-temperature-responsive MYC element, dehydration-responsive MYB element, and GA signaling related WRKY71OS element. To investigate the fine regulation of FAD2 gene, a recombinant FAD2 promoter-GUS construct was introduced into Arabidopsis plants. Histochemical study showed that the promoter was ubiquitously active and responsive not only to exogenous phytohormones including ABA, 24-eBL, and SA but also to darkness, temperature, salt, and sucrose stresses in Arabidopsis seedlings. Consistent with the expression change, treatments with exogenous 24-eBL, ABA, SA, and NaCl resulted in reduction in polyunsaturated FAs in Arabidopsis seedlings. These findings suggest that the FAD2 gene with a wide variety of putative response elements in its promoter is responsive to multiple phytohormones and abiotic stresses and therefore may play an important role in stress responses of Arabidopsis during plant growth and seed development.