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Genes & Diseases 2024年第3期11卷 55-58页

作者： Chengyu Li Deyuan Pan Wan Lin Danxia Deng Yingxin Zhou Hui Zhao Feng Pan Liyan Xu Enmin Li Lin Long The key laboratory of molecular biology for high cancer incidence coastal chaoshan area Department of Biochemistry and Molecular BiologyShantou University Medical CollegeShantouGuangdong 515041China Guangdong Provincial key laboratory of Infectious Diseases and molecular Immunopathology Institute of Oncologic PathologyShantou University Medical CollegeShantouGuangdong 515041China Institute of Basic Medical Science Cancer Research CenterShantou University Medical CollegeShantouGuangdong 515041China Clinical Research Center Shantou Central HospitalShantouGuangdong 515041China

Esophageal cancer is one of the leading causes of cancer death in the world,with approximately half of the new cases occurring in China every year.^(1)Esophageal squamous cell carcinoma(ESCC)is the main subtype,accounting for more than 90%,and the five-year survival rate is less than 10%.Using large-scale genome analysis,many driver mutations and key pathways associated with ESCC have been ***,these genomic signatures have not improved the clinical management of EscC patients,or established effective targeted therapy.^(2)Esophageal cancer still lacks representative molecular markers.

关键词： cancer esophageal Esophageal

Deep proteome profiling promotes whole proteome characterization and drug discovery for esophageal squamous cell carcinoma

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cancer biology & Medicine 2022年第3期19卷 273-277页

作者： Wei Liu Yongping Cui Wen Liu Zhihua Liu Liyan Xu Enmin Li College of Science Heilongjiang Institute of TechnologyHarbin 150050China Guangdong Provincial key laboratory of Infectious Diseases and molecular Immunopathology the Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan AreaShantou University Medical CollegeShantou 515041China Department of Pathology Key Laboratory of Cellular Physiology(Shanxi Medical University)Ministry of EducationTaiyuan 030001China Fujian Provincial key laboratory of Innovative Drug Target Research School of Pharmaceutical SciencesXiamen UniversityXiamen 361005China State key Lab of molecular Oncology National Cancer Center/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China

Proteins are key players in various cellular *** the ultimate executors of cellular processes,they play essential roles in linking genotypes to *** in protein expression and post-translational modifications(PTMs)are closely associated with the initiation and development of cancer,and they carry biological information inaccessible to genomics and *** complements genomic and transcriptomic data,thus enabling comprehensive analysis of cancer pathogenesis and accelerating biomedical research2.

关键词： esophageal linking carry

Fascin lysine 471 acetylation cooperates with serine 39 phosphorylation to inhibit actin-bundling activity and tumor metastasis in esophageal squamous cell carcinoma

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cancer Communications 2022年第7期42卷 668-672页

作者： Fa-Min Zeng Yin-Wei Cheng Jian-Zhong He Xiu-E Xu Lian-Di Liao Li-Yan Xu En-Min Li key laboratory of molecular biology for high cancer incidence coastal chaoshan area Department of Biochemistry and Molecular BiologyShantou University Medical CollegeShantouGuangdong 515041P.R.China Department of Pathology the Fifth Affiliated Hospital of Sun Yat-sen UniversityZhuhaiGuangdong 519000P.R.China Guangdong Provincial key laboratory of Infectious Diseases and molecular Immunopathology Institute of Basic Medical ScienceCancer Research CenterShantou University Medical CollegeShantouGuangdong 515041P.R.China

Dear Editor,Fascin actin-bundling protein 1(Fascin,gene name:FSCN1)is the primary actin-bundling protein that clusters filaments into stable and bundled filopodia[1].In previous studies,elevated levels of Fascin promoted filopodia formation and enhanced the invasive ability of tumor cells,suggesting that Fascin is a target for therapeutic intervention[2,3].Therefore,it is necessary to elucidate the detailed molecular mechanism by which Fascin bundles F-actin filaments.

关键词： Fascin esophageal metastasis

Autoantibodies: Potential clinical applications in early detection of esophageal squamous cell carcinoma and esophagogastric junction adenocarcinoma

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World Journal of Gastroenterology 2019年第34期25卷 5049-5068页

作者： Yi-Wei Xu Yu-Hui Peng Li-Yan Xu Jian-Jun Xie En-Min Li Department of Clinical laboratory Medicine Cancer Hospital of Shantou University Medical CollegeShantou 515041Guangdong ProvinceChina The key laboratory of molecular biology for high cancer incidence coastal chaoshan area Shantou University Medical CollegeShantou 515041Guangdong ProvinceChina Institute of Oncologic Pathology Shantou University Medical CollegeShantou 515041Guangdong ProvinceChina Department of Biochemistry and molecular biology Shantou University Medical CollegeShantou 515041Guangdong ProvinceChina

Esophageal squamous cell carcinoma(ESCC)and esophagogastric junction adenocarcinoma(EGJA)are the two main types of gastrointestinal cancers that pose a huge threat to human *** remains one of the most common malignant diseases around the *** contrast to the decreasing prevalence of ESCC,the incidence of EGJA is rising *** detection represents one of the most promising ways to improve the prognosis and reduce the mortality of these *** approaches for early diagnosis mainly depend on invasive and costly ***-invasive biomarkers are in great need to facilitate earlier detection for better clinical management of ***-associated autoantibodies can be detected at an early stage before manifestations of clinical signs of tumorigenesis,making them promising biomarkers for early detection and monitoring of ESCC and *** this review,we summarize recent insights into the iden-tification and validation of tumor-associated autoantibodies for the early detection of ESCC and EGJA and discuss the challenges remaining for clinical validation.

关键词： Esophageal squamous cell carcinoma Esophagogastric junction adenocarcinoma Biomarker Autoantibody Diagnosis

A shedding soluble form of interleukin-17 receptor D exacerbates collagen-induced arthritis through facilitating TNF-α-dependent receptor clustering

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Cellular & molecular Immunology 2021年第8期18卷 1883-1895页

作者： Sihan Liu Yanxia Fu Kunrong Mei Yinan Jiang Xiaojun Sun Yinyin Wang Fangli Ren Congshan Jiang Liesu Meng Shemin Lu Zhihai Qin Chen Dong Xinquan Wang Zhijie Chang Shigao Yang State key laboratory of Membrane biology School of MedicineTsinghua University100084BeijingChina The key laboratory of molecular biology for high cancer incidence coastal chaoshan area Shantou University Medical CollegeShantou515041GuangdongChina Department of Biochemistry and molecular biology Capital Medical University100069BeijingChina Center for Structural biology School of Life SciencesMinistry of Education Key Laboratory of Protein ScienceTsinghua University100084BeijingChina Department of Biochemistry and molecular biology School of Basic Medical ScienceXi’an Jiaotong University Health Science CenterKey Laboratory of Environment and Genes Related to Diseases(Xi’an Jiaotong University)Ministry of EducationXi’an710061ShanxiChina key laboratory of Protein and Peptide Pharmaceuticals CAS-University of Tokyo Joint Laboratory of Structural Virology and ImmunologyInstitute of BiophysicsChinese Academy of Sciences100101BeijingChina Institute for Immunology and School of Medicine Tsinghua University100084BeijingChina School of Life Sciences Anhui Medical UniversityHefei230032China

Rheumatoid arthritis(RA)is exacerbated by TNF-alpha ***,it remains unclear whether TNF-α-activated TNFR1 and TNFR2 are regulated by extracellular ***,we showed that soluble glycosylated interleukin-17 receptor D(sIL-17RD),which was produced by proteolytic cleavage,enhanced TNF-α-induced *** revealed that IL-17RD shedding was induced by the proteolytic enzyme TACE and enhanced by TNF-αexpression in ***,sIL-17RD was elevated in the sera of arthritic mice and *** sIL-17RD significantly enhanced the TNF-α-induced proinflammatory response by promoting TNF-α-TNFR-sIL-17RD complex formation and receptor clustering,leading to the accelerated development of collagen-induced *** observations revealed that ectodomain shedding of IL-17RD occurred in RA to boost the TNF-α-induced inflammatory *** sIL-17RD may provide a new strategy for the therapy of RA.

关键词： IL-17RD TACE/ADAM17 Ectodomain shedding TNF-αsignaling Arthritis

From start to end: phase separation and transcriptional regulation

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From start to end: phase separation and transcriptional regu...

Down-regulated γ-catenin expression is associated with tumor aggressiveness in esophageal cancer

2021海峡两岸暨港澳精准医学青年博士论坛

作者： Liu Peng En-Min Li Li-Yan Xu The key laboratory of molecular biology for high cancer incidence coastal chaoshan area Shantou University Medical College Department of Biochemistry and molecular biology Shantou University Medical College Institute of Oncologic Pathology Shantou University Medical College

Phase separation is the basis for the formation of membrane-less organelles in cells and is involved in many biological processes. Many biological macromolecules, such as proteins and nucleic acids, exert their biological functions by forming phase-separated condensates, and phase separation is closely related to various human diseases. Gene transcriptional regulation is an indispensable part of gene expression and normal function in cells. Its abnormal regulation often causes the occurrence of different diseases. In recent years, the occurrence of phase separation during transcriptional regulation has become an area of intense research. This review summarizes the process of phase separation involved in heterochromatin formation and chromatin remodeling, transcriptional regulation and post-transcriptional regulation. It provides a reference for understanding gene regulation during cell identity and disease development.

关键词： phase separation heterochromatin formation chromatin remodeling transcriptional regulation post-transcriptional regulation

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World Journal of Gastroenterology 2014年第19期20卷 5839-5848页

作者： Wang-Kai Fang Lian-Di Liao Wei Gu Bo Chen Zhi-Yong Wu Jian-Yi Wu Jian Shen Li-Yan Xu En-Min Li Department of Biochemistry and molecular biology The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan AreaShantou University Medical College Institute of Oncologic Pathology Shantou University Medical College Department of Oncology Surgery Affiliated Shantou Hospital of Sun Yat-sen University

AIM:To evaluate the significance ofγ-catenin in clinical pathology,cellular function and signaling mechanism in esophageal squamous cell carcinoma(ESCC).METHODS:The mRNA expression ofγ-catenin was detected by real-time quantitative reverse transcription-polymerase chain reaction in 95 tissue specimens and evaluated for association with the clinicopathologic characteristics and survival time of patients with *** against humanγ-catenin were used to inhibitγ-catenin *** drop aggregation assay and dispase-based dissociation assay were performed to detect the effect ofγ-catenin on ESCC cell-cell *** assay was performed to determine cell ***-based transcriptional reporter assay(TOPflash)was used to measureβ-catenindependent transcription in cells with reducedγ-catenin *** expression and subcellular localizations ofβ-catenin and E-cadherin were examined using Western blot and immunofluorescence ***:γ-catenin mRNA expression was significantly associated with tumor histological grade(P=0.017)in ***-Meier survival analysis showed thatγ-catenin expression levels had an impact on the survival curve,with lowγ-catenin indicating worse survival(P=0.003).The multivariate Cox regression analysis demonstrated thatγ-catenin was an independent prognostic factor for ***,silencingγ-catenin caused defects in cell-cell adhesion and a concomitant increase in cell migration in both KYSE150 and TE3 ESCC *** of Wnt signaling revealed no activation event associated withγ-catenin ***β-catenin and Triton X-100-insolubleβ-catenin were significantly reduced in theγ-catenin-specific siRNA-transfected KYSE150 and TE3 cells,whereas Triton X-100-solubleβ-catenin was not ***,knocking downγ-catenin expression resulted in a significant decrease of E-cadherin and Triton X-100-insoluble desmocollin-2,along with reducedβ-catenin and E-cadherin membrane localization i

关键词： γ-catenin Esophageal squamous cell carcinoma Indep

Protein-coding genes combined with long noncoding RNA as a novel transcriptome molecular staging model to predict the survival of patients with esophageal squamous cell carcinoma

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cancer Communications 2018年第1期38卷 50-62页

作者： Jin-Cheng Guo Yang Wu Yang Chen Feng Pan Zhi-Yong Wu Jia-Sheng Zhang Jian-Yi Wu Xiu-E Xu Jian-Mei Zhao En-Min Li Yi Zhao Li-Yan Xu key laboratory of molecular biology in high cancer incidence coastal chaoshan area of Guangdong higher Education Institutes Shantou University Medical CollegeShantouGuangdong 515041P.R.China Department of Biochemistry and molecular biology Shantou University Medical CollegeShantouGuangdong 515041P.R.China Departments of Oncology Surgery Shantou Central HospitalAffiliated Shantou Hospital of Sun Yat-Sen UniversityShantouGuangdong 515041P.R.China Institute of Oncologic Pathology Shantou University Medical CollegeShantouGuangdong 515041P.R.China key laboratory of Intelligent Information Processing Advanced Computer Research CenterState Key Laboratory of Computer ArchitectureInstitute of Computing TechnologyChinese Academy of SciencesBeijing 100190P.R.China

Background:Esophageal squamous cell carcinoma(ESCC)is the predominant subtype of esophageal carcinoma in *** study was to develop a staging model to predict outcomes of patients with ***:Using Cox regression analysis,principal component analysis(PCA),partitioning clustering,Kaplan-Meier analysis,receiver operating characteristic(ROC)curve analysis,and classification and regression tree(CART)analysis,we mined the Gene Expression Omnibus database to determine the expression profiles of genes in 179 patients with ESCC from GSE63624 and GSE63622 ***:Univariate cox regression analysis of the GSE63624 dataset revealed that 2404 protein-coding genes(PCGs)and 635 long non-coding RNAs(lncRNAs)were associated with the survival of patients with *** categorized these PCGs and lncRNAs into three principal components(PCs),which were used to cluster the patients into three *** analysis demonstrated that the predictive ability of PCG-lncRNA PCs when applied to new patients was better than that of the tumor-node-metastasis staging(area under ROC curve[AUC]:0.69 vs.0.65,Pmolecular disaggregated model comprising one lncRNA and two PCGs,which we desig-nated as the LSB staging model using CART analysis in the GSE63624 *** LSB staging model classified the GSE63622 dataset of patients into three different groups,and its effectiveness was validated by analysis of another cohort of 105 ***:The LSB staging model has clinical significance for the prognosis prediction of patients with ESCC and may serve as a three-gene staging microarray.

关键词： Long non-coding RNA Protein-coding gene Esophageal squamous cell carcinoma Overall survival Staging model Transcriptome

P300/CBP-associated factor(PCAF)-mediated acetylation of Fascin at lysine 471 inhibits its actin-bundling activity and tumor metastasis in esophageal cancer

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cancer Communications 2021年第12期41卷 1398-1416页

作者： Yin-Wei Cheng Fa-Min Zeng Da-Jia Li Shao-Hong Wang Jian-Zhong He Zhen-Chang Guo Ping-Juan Nie Zhi-Yong Wu Wen-Qi Shi Bing Wen Xiu-E Xu Lian-Di Liao Zhi-Mao Li Jian-Yi Wu Jun Zhan Hong-Quan Zhang Zhi-Jie Chang Kai Zhang Li-Yan Xu En-Min Li The key laboratory of molecular biology for high cancer incidence coastal chaoshan area Department of Biochemistry and Molecular BiologyShantou University Medical CollegeShantouGuangdong 515041P.R.China Institute of Basic Medical Science Cancer Research CenterShantou University Medical CollegeShantouGuangdong 515041P.R.China Shantou Central Hospital Affiliated Shantou Hospital of Sun Yat-sen UniversityShantouGuangdong 515041P.R.China Guangdong Provincial key laboratory of Infectious Diseases and molecular Immunopathology Institute of Oncologic PathologyShantou University Medical CollegeShantouGuangdong 515041P.R.China Tianjin key laboratory of Medical Epigenetics Department of Biochemistry and Molecular BiologySchool of Basic Medical SciencesTianjin Medical UniversityTianjin 300070P.R.China State key laboratory of Natural and Biomimetic Drugs Peking University Health Science CenterBeijing 100191P.R.China School of Medicine Tsinghua UniversityBeijing 100084P.R.China

Background:Fascin is crucial for cancer cell filopodium formation and tumor metastasis,and is functionally regulated by post-translational ***,whether and how Fascin is regulated by acetylation remains *** study explored the regulation of Fascin acetylation and its corresponding roles in filopodium formation and tumor ***:Immunoprecipitation and glutathione-S-transferase pull-down assays were performed to examine the interaction between Fascin and acetyltransferase P300/CBP-associated factor(PCAF),and immunofluorescence was used to investigate their *** in vitro acetylation assay was performed to identify Fascin acetylation sites by using mass spectrometry.A specific antibody against acetylated Fascin was generated and used to detect the PCAF-mediated Fascin acetylation in esophageal squamous cell carcinoma(ESCC)cells using Western blotting by overexpressing and knocking down PCAF *** in vitro cell migration assay was performed,and a xenograft model was established to study in vivo tumor ***-cell imaging and fluorescence recovery after photobleaching were used to evaluate the function and dynamics of acetylated Fascin in filopodium *** clinical significance of acetylated Fascin and PCAF in ESCC was evaluated using ***:Fascin directly interacted and colocalized with PCAF in the cytoplasm and was acetylated at lysine 471(K471)by *** the specific antiAcK471-Fascin antibody,Fascin was found to be acetylated in ESCC cells,and the acetylation level was consequently increased after PCAF overexpression and decreased after PCAF ***,Fascin-K471 acetylation markedly suppressed in vitro ESCC cell migration and in vivo tumor metastasis,whereas Fascin-K471 deacetylation exhibited a potent oncogenic ***,Fascin-K471 acetylation reduced filopodial length and density,and lifespan of ESCC cells,while its deacetylation produced the opp

关键词： acetylation actin-bundling esophageal cancer fascin filopodium formation P300/CBPassociated factor(PCAF) tumor metastasis

Expressions of the Cell Cycle Relative Proteins in Esophageal cancer Tissues

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Journal of cancer Therapy 2018年第11期9卷 857-871页

作者： Sara Khodahemmati Jintao Li Hongwei Liu Maliha Ghaffar Yangjunqi Wang Zhixiang Zhou Yi Zeng Beijing key laboratory of Environmental and Viral Oncology College of Life Science and Bio-Engineering Beijing University of Technology Beijing China Department of Life Science China West Normal University Nanchong China State key laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Beijing China

The aim of the research is to investigate the expression of the cell cycle relative proteins (P53, P16, Cyclin D1, and Ki67) in Esophageal cancer (EC) patients of the chaoshan area, China. In China, chaoshan has the high incidence of EC. Different areas have shown different rate for expression of these proteins in EC. We investigated the expression of p53, p16, cyclinD1, and ki67 for the first time in chaoshan. In this research, DNA was extracted from formalin fixed and paraffin embedded tissues of esophageal cancer (EC) patients. The expression level of proteins cycle was detected by using immunohistochemistry (IHC). And the data was checked by χ2 test or Fisher’s exact test of SPSS17.0. The positive immunohistochemical staining of p53, p16, cyclinD1, and ki67 were observed in 65.7% 39.2%, 69.1%, and 83.5% specimens respectively. There was a positive correlation between p53 positive staining and p16, cyclinD1, ki67 staining at p high correlation with ki67 at p high expression of p53, Cyclin D1 and Ki67 and low expression of P16 and the association of these cell cycle relative proteins in esophageal cancer are new data in chaoshan area of China. Geographical distribution of EC on the molecular basis is revealed in this research.

关键词： Esophageal cancer Regulatory Pathway P53 P16 CyclinD1 ki67