检索结果-南通市图书馆

Contemporary strategies and approaches for characterizing composition and enhancing biofilm penetration targeting bacterial extracellular polymeric substances

引用

Journal of Pharmaceutical Analysis 2024年第4期14卷 506-524页

作者： Lan Lu Yuting Zhao Mingxing Li Xiaobo Wang Jie Zhu Li Liao Jingya Wang key laboratory of medicinal and edible plants resources development of sichuan education department Sichuan Industrial Institute of AntibioticsSchool of PharmacyChengdu UniversityChengdu610000China Meishan Pharmaceutical Vocational College School of PharmacyMeishanSichuan620200China laboratory of Molecular Pharmacology Department of PharmacologySchool of PharmacySouthwest Medical UniversityLuzhouSichuan646000China Hepatobiliary Surgery Langzhong People's HospitalLangzhongSichuan646000China

Extracellular polymeric substances(EPS)constitutes crucial elements within bacterial biofilms,facili-tating accelerated antimicrobial resistance and conferring defense against the host's immune *** precise and effective antibiofilm approaches and strategies,tailored to the specific charac-teristics of EPS composition,can offer valuable insights for the creation of novel antimicrobial ***,in turn,holds the potential to mitigate the alarming issue of bacterial drug *** analysis of EPS compositions relies heavily on colorimetric approaches with a significant bias,which is likely due to the selection of a standard compound and the cross-interference of various EPS *** the pivotal role of EPS in biofilm functionality,it is imperative for EPS research to delve deeper into the analysis of intricate compositions,moving beyond the current focus on polymeric *** ne-cessitates a shift from heavy reliance on colorimetric analytic methods to more comprehensive and nuanced analytical *** this study,we have provided a comprehensive summary of existing analytical methods utilized in the characterization of EPS ***,novel strategies aimed at targeting EPS to enhance biofilm penetration were explored,with a specific focus on high-lighting the limitations associated with colorimetric ***,we have outlined the challenges faced in identifying additional components of EPS and propose a prospective research plan to address these *** review has the potential to guide future researchers in the search for novel compounds capable of suppressing EPS,thereby inhibiting biofilm *** insight opens up a new avenue for exploration within this research domain.

关键词： Analytic strategies and approaches Composition characterization Extracellular polymeric substances(EPS) Promoting biofilm penetration

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Versatile plant genome engineering using anti-CRISPR-Cas12a systems

引用

Science China Life Sciences 2024年

作者： Yao He Shishi Liu Long Chen Dongkai Pu Zhaohui Zhong Tang Xu Qiurong Ren Chuan Dong Yawei Wang Danning Wang Xuelian Zheng Fengbiao Guo Tao Zhang Yiping Qi Yong Zhang Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City Chongqing Key Laboratory of Tree Germplasm Innovation and Utilization School of Life Sciences Southwest University department of Biotechnology School of Life Sciences and Technology Center for Informational Biology University of Electronic Science and Technology of China sichuan Institute of edible Fungi Sichuan Academy of Agricultural Sciences Jiangsu key laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding laboratory/key laboratory of Plant Functional Genomics of the Ministry of education College of AgricultureYangzhou University Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Jiangsu key laboratory of Crop Genetics and Physiology Yangzhou University department of Respiratory and Critical Care Medicine Zhongnan Hospital of Wuhan University key laboratory of Combinatorial Biosynthesis and Drug Discovery Ministry of Education and School of Pharmaceutical Sciences Wuhan University department of Plant Science and Landscape Architecture University of Maryland College Park 20742 Institute for Bioscience and Biotechnology Research University of Maryland

CRISPR-Cas12a genome engineering systems have been widely used in plant research and crop breeding. To date, the performance and use of anti-CRISPR-Cas12a systems have not been fully established in plants. Here, we conduct in silico analysis to identify putative anti-CRISPR systems for Cas12a. These putative anti-CRISPR proteins, along with known anti-CRISPR proteins, are assessed for their ability to inhibit Cas12a cleavage activity in vivo and in planta. Among all anti-CRISPR proteins tested, AcrVA1 shows robust inhibition of Mb2Cas12a and LbCas12a in E. coli. Further tests show that AcrVA1 inhibits LbCas12a mediated genome editing in rice protoplasts and stable transgenic lines. Impressively, co-expression of AcrVA1 mitigates off-target effects by CRISPR-LbCas12a, as revealed by whole genome sequencing. In addition, transgenic plants expressing AcrVA1 exhibit different levels of inhibition to LbCas12a mediated genome editing, representing a novel way of fine-tuning genome editing efficiency. By controlling temporal and spatial expression of AcrVA1, we show that inducible and tissue specific genome editing can be achieved in plants. Furthermore, we demonstrate that AcrVA1 also inhibits LbCas12a-based CRISPR activation(CRISPRa) and based on this principle we build logic gates to turn on and off target genes in plant cells. Together, we have established an efficient anti-CRISPR-Cas12a system in plants and demonstrate its versatile applications in mitigating off-target effects, finetuning genome editing efficiency, achieving spatial-temporal control of genome editing, and generating synthetic logic gates for controlling target gene expression in plant cells.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Synchronous activation of Ag nanoparticles and BiOBr for boosting solar-driven CO_(2)reduction

引用

Chinese Chemical Letters 2023年第6期34卷 252-255页

作者： Gaopeng Liu Lin Wang Bin Wang Xingwang Zhu Jinman Yang Pengjun Liu Wenshuai Zhu Ziran Chen Jiexiang Xia key laboratory of medicinal and edible plants resources of Hainan Province Hainan vocational university of Science and TechnologyHaikou 571126China School of Chemistry and Chemical Engineering Institute for Energy ResearchJiangsu UniversityZhenjiang 212013China department of Architecture and Environment Engineering Sichuan Vocational and Technical CollegeSuining 629000China

Artificial photosynthesis of valuable chemicals from CO_(2)is a potential way to achieve sustainable carbon *** CO_(2)conversion activity is still inhibited by the sluggish charge kinetics and poor CO_(2)***,Ag nanoparticles coupled Bi OBr have been constructed by in-situ photoreduction *** crafting of interface between Ag nanoparticles and Bi OBr nanosheets,achieving an ultra-fast charge *** Bi OBr semiconductor excited electrons and plasmonic Ag nanoparticles generated high-energy hot electrons synchronous accelerates the C=O double bond ***,the optimized Ag/BiOBr-2 heterostructure shows excellent CO_(2)photoreduction activity with CO production of 133.75 and 6.83μmol/g under 5 h of 300 W Xe lamp and visible light(λ>400 nm)irradiation,which is 1.51 and 2.81 folds versus the pristine Bi OBr,*** mechanism of CO_(2)photoreduction was in-depth understood through in-situ FT-IR spectrum and density functional theory *** study provides some new perspectives into efficient photocatalytic CO_(2)reduction.

关键词： Ag nanoparticles BiOBr Hot electrons Charge transfer CO_(2)photoreduction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ce(Ⅲ)-modulation over non-enzymatic Pt/CeO_(2)/GO biosensor with outstanding sensitivity and stability for lactic acid detection

引用

稀土学报:英文版 2023年第9期41卷 1437-1447,I0006页

作者： Luyao Zhang Fuli Tian Huan Li Jiangman Meng Qi Liu Xiaoqian Guo Yun Qiu Jun Zhang Changyan Li College of Chemistry and Chemical Engineering.Inner Mongolia University Hohhot 010021China key laboratory of medicinal and edible plants resources of Hainan Province Hainan Vocational University of Science and TechnologyHaikou 571126China department of Materials Science and Engineering Southern University of Science and TechnologyShenzhen 518055China

A series of non-enzymatic graphene functionalized biosensors was developed via deposition precipitation method for lactic acid(LA) detection,which we re characterized by transmission electron micro scopy(TEM),Raman spectroscopy,X-ray photoelectron spectroscopy(XPS),gas chromatography-mass spectrometry,liquid chromatography-mass spectro metry,and proton nuclear magnetic re sonance(~1H NMR).The electrochemical performances of the non-enzymatic biosensors were measured by means of the electrochemical impedance spectroscopy(EIS) and cyclic voltammetry(CV) *** comprehensive analysis of structures shows that Pt,CeO_(2),and GO components interact with each *** the storing and releasing oxygen,the valence ratio of Ce^(3+)/Ce^(4+) and the number of oxygen vacancies in CeO_(2) change accordingly,which can be conducive to increasing electronic transmission capacity and finally leads to the improvement of electrocatalytic *** them,the Pt/CeO_(2)/GO biosensor containing 0.47 at% platinum exhibits an excellent electrochemical detection performance with high sensitivity of 12.3 μA·L/(mmol·cm^(2)) and a low limit of detection(LOD) of 5.12 μmol/L in a wide linear range from 10 to 900 μmol/*** addition,the proposed biosensor possesses a promising anti-interference capability,as well as high stability and good reproducibility,which was assessed by testing the cyclic voltammogram in 0.1 mol/L lactic acid one year *** underlying mechanism was proposed for electrochemical oxidation of LA to carbon dioxide and acetic acid with the synergistic effect among Pt,CeO_(2),and ***,the results of the standard addition method in real samples(human serum and urine samples) reveal that the lactic acid detection of the non-enzymatic Pt/CeO_(2)/GO biosensor is accompanied by high ***,it will be a valuable biosensor for in vitro detection of lactic acid level in clinical samples.

关键词： Non-enzymatic biosensor Lactic acid Graphene functionalized Pt/CeO_(2) Oxygen vacancy Synergistic effect Rare earths

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Oxygen vacancy enhanced biomimetic superoxide dismutase activity of CeO_(2)-Gd nanozymes

引用

Journal of Rare Earths 2021年第9期39卷 1108-1116页

作者： Xiangcheng Shi Jingjie Yang Xintong Wen Fuii Tian Changyan Li College of Chemistry and Chemical Engineering Inner Mongolia UniversityHohhot010021China department of Chemistry National University of Singapore.Singapore 117543Singapore key laboratory of medicinal and edible plants resources of Hainan Province Hainan Vocational University of Science and TechnologyHaikou 571126China

Cerium oxide-based nanozymes have recently drawn much attention in the field of biomedical and antioxidant applications,because of their unique regenerative or autocatalytic ***,we studied the biomimetic superoxide dismutase(SOD)nanozymes CeO_(2)-Gd that combines the fluorescence properties of rare earth Gd with the antioxidant properties of CeO_(2)nanoparticles,which was prepared via facile *** structure and composition of the CeO_(2)-Gd were measured and verified by X-ray powder diffraction(XRD),Raman spectroscopy,transmission electron microscopy(TEM),energydispersive X-ray spectroscopy(EDX),and X-ray electron spectroscopy(XPS).Confocal microscopy was used to image *** performance and cell viability of these nanozymes were measured in vitro using BGC-803 ***_(2)-Gd nanozymes with a higher Ce^(3+)/Ce^(4+)ratio show higher superoxide dismutase(SOD)mimetic *** antioxidant activity and fluorescence properties of CeO_(2)-Gd in BGC-803 cancer cells are enhanced by oxygen vacancies generated by doping rare-earth elements *** work may guide the future design of CeO_(2)-Gd-based biomimetic nanozymes for anticancer and antioxidant applications.

关键词： Antioxidant enzyme CeO_(2)-Gd nanozymes Superoxide dismutase Biomimetic nanozymes Oxygen vacancy Rare earths

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Synthesis of oleanolic disaccharide derivatives

引用

Chinese Chemical Letters 2012年第4期23卷 403-406页

作者： Shu Chen Pei Jian Bo Wu Fan Lei Shan Qian Li Hai Yong Wu key laboratory of Drug Targeting and Drug Delivery System of the education Ministry Department of Medicinal ChemistryWest China School of PharmacySichuan UniversityChengdu 610041China Bioengineering College Xihua UniversityChengdu 610039China

Oleanolic acid 1 exists widely in food,medicinal herbs and other plants and possesses many attractive biological activities. Recently,some oleanolic derivatives were reported to have interesting anti-tumor ***,using association principle of drug design,OA disaccharide 17 was designed and synthesized by coupling a-L-rhamnopyranosyl-（1→2）-a-L-arabinopyranosyl disaccharide moiety,which has proved to be a unique sugar sequence with strongly antitumor activity,and modified OA with a nitrile at C-17.

关键词： Oleanolic acid Rhamnopyranosyl Arabinopyranosyl Anti-tumor Association principle

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Diversity,bioactivities,and metabolic potentials of endophytic actinomycetes isolated from traditional medicinal plants in sichuan,China

引用

Chinese Journal of Natural Medicines 2015年第12期13卷 942-953页

作者： QIU Peng FENG Zhi-Xiang TIAN Jie-Wei LEI Zu-Chao WANG Lei ZENG Zhi-Gang CHU Yi-Wen TIAN Yong-Qiang key laboratory of Leather Chemistry and Engineering Ministry of Education and College of Light IndustryTextile & Food EngineeringSichuan University department of Pharmaceutical and Biological Engineering School of Chemical EngineeringSichuan University sichuan Industrial Institute of Antibiotics Chengdu University

The present study was designed to determine the taxonomic diversity and metabolic activity of the actinomycetes community, including 13 traditional medicinal plants collected in sichuan province, China, using multiple approaches such as morphological and molecular identification methods, bioactivity assays, and PCR screening for genes involved in antibiotics biosynthesis. 119 endophytic actinomycetes were recovered; 80 representative strains were chosen for 16 S r RNA gene partial sequence analyses, with 66 of them being affiliated to genus Streptomyces and the remaining 14 strains being rare actinomycetes. Antimicrobial tests showed that 12(15%) of the 80 endophytic actinomycetes displayed inhibitory effects against at least one indicator pathogens, which were all assigned to the genus Streptomyces. In addition, 87.5% and 58.8% of the isolates showed anticancer and anti-diabetic activities, respectively. Meanwhile, the anticancer activities of the isolates negatively correlated with their anti-diabetic activities. Based on the results of PCR screening, five genes, PKS-I, PKS-II, NRPS, ANSA, and oxy B, were detected in 55.0%, 58.8%, 90.0%, 18.8% and 8.8% of the 80 actinomycetes, respectively. In conclusion, the PCR screening method employed in the present study was conducive for screening and selection of potential actinomycetes and predicting potential secondary metabolites, which could overcome the limitations of traditional activity screening models.

关键词： Endophytic actinomycetes medicinal plants Diversity Bioactivity PCR screening

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Community assemblage of free-living diazotrophs along the elevational gradient of Mount Gongga

引用

Soil Ecology Letters 2019年第3期1卷 136-146页

作者： Yansu Wang Chaonan Li Zehao Shen Junpeng Rui Decai Jin Jiabao Li Xiangzhen Li key laboratory of Biotechnology for medicinal plants of Jiangsu Province and School of Life Sciences Jiangsu Normal UniversityXuzhou 221116China key laboratory of Environmental and Applied Microbiology CAS/Environmental Microbiology Key Laboratory of Sichuan ProvinceChengdu Institute of BiologyChinese Academy of SciencesChengdu 610041China department of Ecology Ministry of Education Key Laboratory for Earth Surface ProcessesPeking UniversityBeijing 100871China key laboratory of Environmental Biotechnology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China

Mountain systems are unique for studying the responses of species distribution and diversity to environmental changes along elevational *** is well known that free-living diazotrophic microorganisms are important to nitrogen cycling in mountain ***,the elevational patterns of free-living diazotrophs and the underlying ecological processes in controlling their turnover along broader gradients are less well ***,we investigated the pattern of diazotrophic diversity along the elevational gradient(1800 m-4100 m)in Mount Gongga of *** results showed that the α-diversity of diazotrophs did not change with the elevation from 1800 m to 2800 m,but decreased at elevations above 3000 *** diversity pattern was driven mainly by soil total carbon,nitrogen,and plant *** diazotrophic taxa showed differential abundance-elevation *** processes determining diazotrophic community assemblage shift along the *** processes were relatively stronger at both low and high elevations,whereas stochastic processes were stronger at the middle *** study also suggested a strong relationship among aboveground plants and diazotrophs,highlighting their potential interactions,even for free-living diazotrophs.

关键词： Diazotrophs Community assemblage Elevational gradient Mount Gongga

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A comprehensive review on mechanism of natural products against Staphylococcus aureus

引用

Journal of Future Foods 2022年第1期2卷 25-33页

作者： Yingying Zhao Jinfeng Wei Changqin Li Adel F.Ahmed Zhenhua Liu Changyang Ma National R&D Center for edible Fungus Processing Technology Henan UniversityKaifeng 475004China Henan Kaifeng College of Science Technology and Communication(Minsheng College Henan University)Kaifeng 475004China Functional Food Engineering Technology Research Center of Henan Province Kaifeng 475004China medicinal and Aromatic plants Researches department Horticulture Research InstituteAgricultural Research CenterCairo 11435Egypt Joint International Research laboratory of Food&Medicine Resource Function of Henan Province Henan UniversityKaifeng 475004China

The abuse of antibiotics seriously threatens the therapeutic *** products from plants,antimicrobial peptides and micro-organisms have antibacterial activity against Staphylococcus *** mechanism of natural products reported in recent years on *** includes six types,which are the acting on bacterial cell wall,cytomembrane,metabolic pathway,protein,nucleic acid,biofilm and quorum sensing system to achieve antibacterial *** article summarized the mechanism of natural products reported in recent years on *** is found that the natural products acting on cell wall or cell wall synthesis pathway key enzymes are relatively safe and highly *** has the prospect of developing into medicine and provides the clue to investigate and utilize natural products in antibacterial activity.

关键词： Natural products Staphylococcus aureus Antibacterial mechanism Enzymes Cell wall

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Comparative analysis of chemical constituents, antimicrobial and antioxidant activities of ethylacetate extracts of Polygonum cuspidatum and its endophytic actinomycete, Streptomyces sp. A0916

引用

Chinese Journal of Natural Medicines 2016年第2期14卷 117-123页

作者： WANG Lei QIU Peng LONG Xiu-Feng ZHANG Shuai ZENG Zhi-Gang TIAN Yong-Qiang key laboratory of Leather Chemistry and engineering Ministry of Education and College of Light Industry Textile & Food Engineering Siehuan University Chengdu 610065 China department of Pharmaceutical and Biological Engineering School of Chemical Engineering Sichuan University Chengdu 610065 China sichuan Industrial Institute of Antibiotics Chengdu 610052 China

The present study investigated the chemical composition of ethylacetate extracts from an endophytic actinomycete Streptomyces sp. A0916 and its host Polygonum cuspidatum. A comparative analysis of the antimicrobial and antioxidant properties of the extracts was also conducted. 32 compounds of P. cuspidatum and 23 compounds of Streptomyces sp. A0916 were isolated and identified by GC/MS. Antimicrobial activities of the extracts were evaluated using eight microbial strains(3 Gram-positive bacteria, 3 Gram-negative bacteria, and 2 fungi). The Streptomyces sp. A0916 extracts showed a wide range of antimicrobial activities and presented greater antimicrobial effectiveness than the P. cuspidatum extracts. The minimum inhibitory concentration(MIC) of Streptomyces sp. A0916 extracts against the ampicillin-resistant strain Enterococcus faecium SIIA843 was 32 μg·m L-1. Furthermore, the extracts had greater antimicrobial effect against Gram-positive bacteria than Gram-negative bacteria. Finally, the antioxidant activity of the Streptomyces sp. A0916 extracts was equal to that of the P. cuspidatum extracts. In conclusion, our results suggest that the endophytic actinomycetes of the medicinal plants are an important source of bioactive substances.

关键词： Polygonum cuspidatum Endophytic actinomycete Ethylacetate extracts GC/MS Antimicrobial activity Antioxidant activity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：