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Syntheses and cofluorescence of complexes of Eu(Ⅲ)/Y(Ⅲ) with terephthalic acid, 2-thenoyltrifluoroacetone and trioctylphosphine oxide

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中国有色金属学会会刊：英文版 2007年第3期17卷 638-643页

作者：赵学辉黄可龙刘素琴焦飞鹏刘志国胡舜钦李朝建 School of Chemistry and Chemical Engineering Central South University key laboratory of green packaging and Application biological nanotechnology of hunan province Hunan University of Technology

A series of Eu( Ⅲ )/Y( Ⅲ ) complexes of terephthalic acid(TPA) with 2-thenoyltrifluoroacetone(HTTA) and trioctylphosphine oxide(TPPO) were synthesized. Compositions of these complexes are revealed to be Eu2(1-x)Y2x(TPA)(TTA)4- (TPPO)4 or Eu1-xYx(TPA)(TTA)(TPPO)2. Their IR spectra, fluorescent spectra and the thermal and optical stability were studied. The fluorescent excitation spectra of these complexes show more broad excitation bands than those of Eu2(TPA)3(TPPO)4 and Eu(TTA)3(TPPO)2 corresponding to their formations. In addition, both the binuclear complex EuY(TPA)(TTA)4(TPPO)4 and the chain complex Eu0.4Y0.6(TPA)(TTA)(TPPO)2 present higher thermal stability and better optical stability than the mononuclear complex Eu(TTA)3(TPPO)2 does. And their thermal and optical stabilities are preferably interpreted from the binuclear structure together with the chain polynuclear structure of the complexes. The fluorescence enhancement of cofluorescence Y3+ ions to the Eu(Ⅲ) complexes is clear. The optimum content of Y3+ is 0.6 (molar fraction) for the chain complexes Eu1-xYx(TPA)(TTA)- (TPPO)2 and 0.5 for the binuclear complexes Eu2(1-x)Y2x(TPA)(TTA)4(TPPO)4. The formation of polynuclear structure of the complex Eu1-xYx(TPA)(TTA)(TPPO)2 appears to be responsible for the good cofluorescence effect of Y3+ ions.

关键词：铕元素对酞酸 2-噻吩甲酰基三氟丙酮合成方法

Isolation of Mucor circinelloides Z4 and Mucor racemosus Z8 from heavy metal-contaminated soil and their potential in promoting phytoextraction with Guizhou oilseed rap

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Journal of Central South University 2015年第1期22卷 88-94页

作者：朱生翠汤建新曾晓希魏本杰杨少迪黄斌 hunan key laboratory of green packaging and biological nanotechnology Hunan University of Technology Meteorological Institute of Qinghai province School of Metallurgy and Environment Central South University

Fungi Z4 and Z8, isolated from the heavy metal polluted soil, have strong resistance to Cd and Pb. The strains were identified on the base of their morphology and internal transcribed spacers(ITS) region. Pot experiments were conducted to study the effect of two strains(Z4 and Z8) on the growth and accumulation of Cd and Pb of Guizhou oilseed rape. The results show that strains Z4 and Z8 belong to Mucor circinelloides and Mucor racemosus, respectively. The heights of Guizhou oilseed rape inoculated with strain Z8 increase by 47.90% than the control. The highest fresh mass is found in the plant with Z4/Z8, which is enhanced by160.81%. Pot experiments show that Z4/Z8 inoculums can accelerate accumulation of heavy metals in the plant. The contents of Cd and Pb are increased by 117.60% and 63.48%, respectively. Meanwhile, the heavy metal concentrations in potting soil with the two strains are found to be lower than those of the control, and the concentrations of Cd and Pb are decreased by 60.57% and 27.12%,respectively.

关键词： phytoextraction fungus cadmium lead oilseed rape

Syntheses and applications of Eu(Ⅲ) complexes of 2-thienyltrifluoroacetonate, terephthalic acid and phenanthroline as light conversion agents

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Journal of Central South University of Technology 2007年第1期14卷 62-67页

作者：赵学辉黄可龙焦飞鹏杨幼平李朝建刘志国胡舜钦 School of Chemistry and Chemical Engineering Central South University key laboratory of green packaging and Application biological nanotechnology of hunan province Hunan University of Technology

A series of europium（Ⅲ） complexes of 2-thienyltrifluoroacetonate （HTTA）, terephthalic acid （TPA） and phenanthroline （Phen） were synthesized. The new complexes Eu（TPA）（TTA）Phen and EUE（TPA）（TTA）4Phen2 were characterized by elemental analysis, IR spectrum, scanning electron microscope and thermal stability analysis. The results show that the thermal stability of the Eu（Ⅲ） complexes increases in the following order： the mononuclear complex Eu（TTA）3Phen, the binuclear complex EUE（TPA）（TTA）4Phen2, the chain polynuclear complex Eu（TPA）（TTA）Phen. And the formation of the binuclear/polynuclear structure of the new complexes appears to be responsible for the enhancement of their thermal and optical stability. In addition, The fluorescence excitation spectra of these new complexes show more broad excitation bands than that of the complex Eu（TTA）3Phen corresponding to their formation. The enhancement of Eu^3＋ fluorescence in the new complexes can be observed by the addition of Gd^3＋. The bright red luminescent plastics can be obtained when the complex EuGd（TPA）（TTA）4Phen2 is added above 0.5% （mass fraction）.

关键词： fluorescent properties europium terephthalic acid 2-thienyltrifluoroacetonate

Size-tunable synthesis of gold nanorods using pyrogallol as a reducing agent

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Science China Chemistry 2015年第11期58卷 1759-1765页

作者： Yuanfu Huang Kai Xia Nongyue He Zhuoxuan Lu Liming Zhang Yan Deng Libo Nie Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in hunan province hunan key laboratory of green packaging and Application of biological nanotechnology hunan University of Technology State key laboratory of Bioelectronics School of biological Science and Medical Engineering Southeast University

A novel and facile seed-mediated method for the preparation of monodispersed gold nanorods(GNRs) is presented by introducing pyrogallol as a reductant. Fast Fourier transformation of high-resolution transmission electron microscopy reveals that the synthesized GNRs are single crystalline. The longitudinal surface plasmon resonance of GNRs can be finely tuned by varying silver ion concentrations or seed amounts. Also, both thick(diameter >30 nm) and thin(diameter <10 nm) GNRs with exceptional monodispersity can be well prepared by this method. These findings indicate that this method has a greater performance in controlling the morphology of GNRs than that of traditional approach with ascorbic acid as a reductant.

关键词： gold nanorods seed-mediated pyrogallol size tunability

Isolation,characterization and extraction of mer gene of Hg^(2+) resisting strain D_2

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Transactions of Nonferrous Metals Society of China 2010年第3期20卷 507-512页

作者：曾晓希汤建新蒋佩刘宏伟戴志敏刘学端 School of Mineral Processing and Bioengineering Central South University hunan key laboratory of green packaging and biological nanotechnology Hunan University of Technology

Mercury-resistant strain D2 was isolated from mercury-contaminated soil and investigations on its 16S rDNA sequence,growth,minimal inhibitory concentrations(MICs) of metals,antibiotic susceptibility and mer gene were *** strain D2 can grow in the medium containing 60 mg/L mercury *** presents more than 99% identity of 16S rRNA gene with Pseudomonas aeruginosa,and exhibits high MIC values for heavy metals and a large spectrum antibiotics *** mer RT gene sequence was amplified from *** D2 is identified as Pseudomonas aeruginosa and the resistance to mercuric ion is related to chromosome.

关键词： isolation mercury-resistant strain Pseudomonas aeruginosa mer RT

In situ synthesis of DNA micro-arrays using typography technique

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Science China Chemistry 2007年第1期50卷 75-83页

作者： TANG JianXin 1,2 ,HE NongYue 1,2,3 &LI Song 1 1State key laboratory of Bioelectronics,Southeast University,Nanjing 210096,China 2key laboratory of green packaging and Application of biological nanotechnology of hunan province,hunan University of Tech- nology,Zhuzhou 412008,China 3Zhongshan Huoju Polytechnic,Zhongshan 528436,China State key laboratory of Bioelectronics Southeast University Nanjing China key laboratory of green packaging and Application of biological nanotechnology of hunan province Hunan University of Technology Zhuzhou China Zhongshan Huoju Polytechnic Zhongshan China

A novel typography technique was developed to in situ synthesize oligonucleotide arrays on glass slide,which has the celerity,high spatial resolution,lower cost,reliable operation,and high synthetic *** principle and process of the typography technique for fabricating gene-chips have been described in detail.A suit of poly(terafluoroethylene)devices for synthesizing oligonucleotide arrays were designed and prepared,and the fiber tubes with a number of nano-or micron-channels were em- *** oligonucleotide arrays of 16 and 160 features with four different probes were synthesized using the typography *** four specific oligonucleotide probes including the matched and the mismatched by the fluorescent target sequence gave obviously different hybridization fluorescent *** was indicated that the gene-chip fabricated by the typography method could be used to rapidly screen single-nucleotide polymorphisms(SNP)and to detect mutations.

关键词： typography oligonucleotide in situ synthesis DNA microarrays

In situ Synthesis of Oligonucleotide and Detection of Gold-label-silver-stain on PTFE Slices

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Chinese Journal of Chemistry 2005年第9期23卷 1247-1252页

作者：谭美军汤建新陈洪 key laboratory of green packaging and biological nanotechnology of hunan province Hunan Polytechnic University Zhuzhou Hunan 412008 China key laboratory of Non-timber Breeding and Cultivation of Ministry of Forestry of China Central South Forestry University Zhuzhou Hunan 412006 China

Poly（tetrafluoroethylene）（PTFE） was treated with plasma in a mixture of nitrogen and hydrogen （1 ： 2 in volume）. X-ray photoelectron spectroscopy （XPS） demonstrated the success of amino group grafting. The as-treated PTFE slices were successively applied to the in situ synthesis of oligonucleotides. With the detection of gold-label-silver-stain, the hybridization signals were recorded with a gel document and analysis system. A target DNA concentration as low as 10 pmol/L could be detected. The complementary and mismatched sequences were distinguished clearly, and the ratio of background-subtracted gray scale values for perfect match ： 1 base mismatch ： 2 base mismatch ： 3 base mismatch was 72 ： 44.4 ： 22.5 ： 11.4. The sensitivity of in situ synthesis system was 1 order of magnitude higher than that of spotting system, and the signal of the former was about 1.5 times stronger than that of the latter under the same target DNA concentration. These plasma modified PTFE slices might open novel prospects for the in situ synthesis of DNA micro-arrays.

关键词： oligonucleotide gold-label-silver-stain in situ synthesis plasma poly(tetrafluoroethylene)

Simultaneous extraction of DNA and RNA from Escherichia coli BL 21 based on silica-coated magnetic nanoparticles

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Science China Chemistry 2015年第11期58卷 1774-1778页

作者： Jiuhai Wang Zeeshan Ali Nianyue Wang Wenbiao Liang Hongna Liu Fu Li Haowen Yang Lei He Libo Nie Nongyue He Zhiyang Li State key laboratory of Bioelectronics School of biological Science and Medical Engineering Southeast University Clinical laboratory the Second Hospital of Nanjing Jiangsu province Blood Center Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in hunan province hunan key laboratory of green packaging and Application of biological nanotechnology hunan University of Technology Department of laboratory Medicine the Second Affiliated Hospital of Nanjing Medical University

The extraction of nucleic acid is recognized as one of the most essential steps in molecular biology for initiating other downstream applications such as sequencing, amplification, hybridization, and cloning. Many commercial kits and methods are currently available that allow the extraction of only one type of nucleic acids-DNA or RNA. However, in parallel clinical detection of several diseases, a method for simultaneous extraction of both DNA and RNA from the same source is needed in such cases. In this study, a method for simultaneous extraction of DNA and RNA from bacteria based on magnetic nanoparticles(MNPs) was described. Lysis buffers were prepared to help the nucleic acid released and adsorbed to MNPs. Then, two washing buffers were used to remove the contamination of proteins and carbohydrates. The nucleic acids were finally eluted by Deoxyribonuclease(DNase) and Ribonucleases(RNase) free water. Different factors which might affect the purification of the nucleic acid were investigated, and the quantity and quality parameters of the nucleic acid were also recorded. The DNA and RNA extracted from bacteria were then respectively subjected to polymerase chain reaction(PCR) and reverse transcription PCR(RT-PCR) to further confirm its quality. The results indicated that our method can be successfully used to simultaneously extract DNA and RNA from bacteria.

关键词： nucleic acids simultaneous extraction magnetic nanoparticles (MNPs) bacteria

A new method for improving the accuracy of miRNA detection with NaYF4：Yb,Er upconversion nanoparticles

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Science China Chemistry 2017年第1期60卷 157-162页

作者： Lanlan Mao Zhuoxuan Lu Nongyue He Liming Zhang Yan Deng Deming Duan Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in hunan province Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology Hunan University of Technology Zhuzhou 412007 China key laboratory of Protein and Peptide Pharmaceuticals Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China State key laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University Nanjing 210096 China

MicroRNAs(miRNAs)are small noncoding RNAs,which play a central role in gene expression regulation and have been considered as excellent biomarker candidates for clinical diagnosis and *** far,many miRNAs detection methods require polymerase chain reaction(PCR)amplification following reverse transcription of *** processes are complicated and *** this work,we have developed a simpler method for miRNA detection based on base stacking hybridization happening on the surface of NaYF_4:Yb,Er upconversion *** this method,the fluorescence of NaYF_4:Yb,Er upconversion nanoparticles were functionalized as a reference standard,which can improve the accuracy of miRNA *** the basis of these findings,we suggest this novel approach for miRNA detection could be applied as an accurate and specific technique for miRNAs detection.

关键词： microRNAs detection NaYF4:Yb Er reference standard base stacking hybridization