A new method for improving the accuracy of miRNA detection with NaYF4：Yb,Er upconversion nanoparticles

作     者：Lanlan Mao Zhuoxuan Lu Nongyue He Liming Zhang Yan Deng Deming Duan 

作者机构：Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology Hunan University of Technology Zhuzhou 412007 China Key Laboratory of Protein and Peptide Pharmaceuticals Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China State Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University Nanjing 210096 China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2017年第60卷第1期

页      面：157-162页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070205[理学-凝聚态物理] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0702[理学-物理学] 

基　　金：the National Natural Science Foundation of China(61301039,21205036,31270908,61271056, 31540018) the Hunan Provincial Natural Science Foundation of China (13JJ4091) the Scientific Research Fund of Hunan Provincial Education Department(13A003) China Postdoctoral Science Foundation funded project(2014T70459) the Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province[(2013) 448] 

主　　题：microRNAs detection NaYF4:Yb Er reference standard base stacking hybridization 

摘      要：MicroRNAs(miRNAs)are small noncoding RNAs,which play a central role in gene expression regulation and have been considered as excellent biomarker candidates for clinical diagnosis and *** far,many miRNAs detection methods require polymerase chain reaction(PCR)amplification following reverse transcription of *** processes are complicated and *** this work,we have developed a simpler method for miRNA detection based on base stacking hybridization happening on the surface of NaYF_4:Yb,Er upconversion *** this method,the fluorescence of NaYF_4:Yb,Er upconversion nanoparticles were functionalized as a reference standard,which can improve the accuracy of miRNA *** the basis of these findings,we suggest this novel approach for miRNA detection could be applied as an accurate and specific technique for miRNAs detection.