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Research Progress on the Integrated Detection Technology for forensic Deoxyribonucleic Acid Genetic Markers and Ribonucleic Acid Molecular Markers

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Journal of forensic Science and Medicine 2023年第1期9卷 64-69页

作者： Lei Miao Jia-Hui Yuan Ke-Lai Kang Jie Zhao Chi Zhang Le Wang key laboratory of forensic genetics of ministry of public security Institute of Forensic ScienceMinistry of Public SecurityBeijingChina Department of forensic Medicine School of Forensic MedicineKunming Medical UniversityKunmingYunnanChina

Deoxyribonucleic acid(DNA)genetic markers and ribonucleic acid(RNA)molecular markers have been widely used in forensic practices including individual identification,parentage testing,body fluid identification,determination of the age of stains,and molecular pathological *** information of biological evidence and their interrelation could be revealed by the integrated detection of DNA/RNA *** integrated detection workflow aims to simplify working procedures,reduce time consuming and save valuable samples collected from crime ***-generation sequencing(NGS)may be an effective method for integrated DNA/RNA *** this review,DNA/RNA co-extraction strategies,simultaneous detection methods based on capillary electrophoresis were *** on NGS-based integrated detection methods of DNA and RNA markers was reviewed to provide a reference for forensic medicine researches and applications.

关键词： forensic genetics deoxyribonucleic acid ribonucleic acid integrated detection methods next-generation sequencing

Twenty-seven continental ancestry-informative SNP analysis of bone remains to resolve a forensic case

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forensic Sciences Research 2019年第4期4卷 364-366页

作者： Qifan Sun Li Jiang Guangfeng Zhang Jing Liu Lei Zhao Wenting Zhao Caixia Li key laboratory of forensic genetics Beijing Engineering Research Center of Crime Scene Evidence ExaminationInstitute of Forensic ScienceMinistry of Public SecurityBeijingChina

We employed our previously developed 27-plex ancestry-informative single nucleotide polymorphism(SNP)panel to infer the ancestral components of bone remains of a possible foreign pilot found in south-western *** ancestry assignment of this unknown individual,we first obtained the 27-SNP genotype of the ***,based on a reference database of 3081 individuals from 33 populations,we calculated the match probability and likelihood ratio using the self-developed software program forensic *** ancestral components of this individual were calculated by structure at K=3.A complete profile was obtained for the individual using our multiplexed SNP *** European population was within one order of magnitude of the highest *** major ancestral component of this individual was 97.6%European.

关键词： forensic science skeletal remains ancestry inference SNPs multiplex assay capillary electrophoresis

Degradation ofβ‑Actin mRNA and 18S rRNA in Mouse Spleen Cells after Death

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Journal of forensic Science and Medicine 2019年第3期5卷 123-129页

作者： Zhiyuan An Feng Li Dong Zhao key laboratory of forensic genetics of ministry of public security Institute of Forensic ScienceMinistry of Public SecurityBeijingChina Collaborative Innovation Center of Judicial Civilization ChinaKey Laboratory of Evidence Science(China University of Political Science and Law)Ministry of EducationBeijingChina public security Bureau of Huaian City JiangsuChina

We observed degradation ofβ‑actin mRNA and 18S rRNA in mouse spleen cells under constant temperature conditions in the different temperature group during postmortem intervals(PMIs)of 0-72 ***‑nine mice were sacrificed by cervical dislocation and kept at constant temperatures of 10℃,15℃,20℃,25℃,and 30℃.From 0 to 72 h after death,total RNA in spleen cells was extracted every 6 *** cycle threshold(Ct)values ofβ‑actin mRNA and 18S rRNA were obtained by real‑time‑quantitative polymerase chain *** results showed that,under the conditions of different and constant temperatures after mouse death at 72 h,the Ct values ofβ‑actin and 18S,Ct ratios ofβ‑actin to 18S,and relative ratios ofβ‑actin to 18S were significantly correlated with *** addition,the relative degradation rates ofβ‑actin and 18S appeared to change from fast to slow with the increase of *** interpolation and fitting analysis of the data,we obtained a ternary quintic equation of the relationship between the change in the relative ratios and PMI,which can be used to infer PMI within a certain temperature range(10℃-30℃).

关键词： 18S rRNA forensic pathology interpolation function postmortem interval β‑actin mRNA

The Impact of Different Stain Carriers on the mRNA Profiling from Bloodstains

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Journal of forensic Science and Medicine 2022年第2期8卷 76-79页

作者： Hemiao Zhao Qingluan Lin Qi Zhang Jing Chen Zheng Tu Ruiqin Yang Lan Hu Chong Wang Division of forensic genetics Key Laboratory of Forensic GeneticsInstitute of Forensic ScienceMinistry of Public Security Qionghai Police Bureau Hainan ProvinceChina School of Crime Investigation Chinese People's Public Security UniversityBeijing

Unlike DNA profiling,mRNA profiling is greatly affected by external *** analyze the influence of different stain carriers on the detectability of mRNA markers from bloodstains,this study examined 10 carriers,including a knife,cotton swab,paper,plastic,leather,cement,chopsticks,clothes,ceramic block,and *** detecting five specific mRNA markers(HBA,HBB,ALAS2,GYPA,and SPTB)and the housekeeping gene B2M in peripheral blood samples,no statistically significant differences in the effects of the carriers were *** results suggest that when performing mRNA testing on bloodstains,the effect of the stain carrier has little influence.

关键词： Bloodstains mRNA profiling stain carrier

Validation of the DNATyper^(TM)15 PCR Genotyping System for forensic Application

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Journal of forensic Science and Medicine 2015年第1期1卷 8-15页

作者： Jian Ye Chengtao Jiang Xingchun Zhao Le Wang Caixia Li Anquan Ji Li Yuan Jing Sun Shuaifeng Chen Institute of forensic Science Ministry of Public Security Beijing Engineering Research Center of Crime Scene Evidence Examination key laboratory of forensic genetics Ministry of Public Security Fada Institute of forensic Medicine and Science key laboratory of Evidence Science China University of Political Science and LawMinistry of Education Department of Investigation People’s Public Security University of ChinaBeijingChina

We describe the optimization and validation of the DNATyper^(TM)15 multiplex polymerase chain reaction(PCR)genotyping system for autosomal short tandem repeat(STR)amplification at 14 autosomal loci(D6S1043,D21S11,D7S820,CSF1PO,D2S1338,D3S1358,D13S317,D8S1179,D16S539,Penta E,D5S818,vWA,D18S51,and FGA)and amelogenin,a sex‑determining *** DNATyper^(TM)15 assay variables were optimized,including hot start Taq polymerase concentration,Taq polymerase activation time,magnesium concentration,primer concentration,annealing temperature,reaction volume,and cycle *** performance of the assay was validated with respect to species specificity,sensitivity to template concentration,stability,accuracy,influence of the DNA extraction methods,and the ability to genotype the mixture *** performance of the DNATyper^(TM)15 system on casework samples was compared with that of two widely used STR amplification kits,Identifiler^(TM)(Applied Biosystems,Carlsbad,CA,USA)and PowerPlex 16®(Promega,Madison,WI,USA).The conditions for PCR‑based DNATyper^(TM)15 genotyping were *** from forensically relevant nonhuman DNA was not found to impact genotyping results,and full profiles were generated for all the reactions containing≥0.125 ng of DNA *** significant difference in performance was observed even after the DNATyper^(TM)15 assay components were subjected to 20 freeze‑thaw *** performances of DNATyper^(TM)15,Identifiler^(TM),and PowerPlex 16®were comparable in terms of sensitivity and the ability to genotype the mixed samples and case‑type samples,with the assays giving the same genotyping results for all the shared *** DNA extraction methods did not affect the performance of any of the *** results demonstrate that the DNATyper^(TM)15 system is suitable for genotyping in both forensic DNA database work and case‑type samples.

关键词： DNATyper^(TM)15 kit short tandem repeats(STRs) validation

forensic Investigation of Atypical Asphysia

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Journal of forensic Science and Medicine 2018年第4期4卷 233-237页

作者： Zhe Cao Zhiyuan An Xiaoning Hou Dong Zhao Anshan public security Bureau AnshanChina key laboratory of Evidence Science(China University of Political Science and Law) Ministry of EducationChinaCollaborative Inn ovation Center of Judicial CivilizationChina key laboratory of forensic genetics of ministry of public security Institute of Forensic ScienceMinistry of Public SecurityBeijingChina

Smothering,choking,confined spaces,traumatic asphyxia,positional asphyxia,and other kinds of atypical mechanical asphyxia are not rare in forensic ***,these are not commonly well demonstrated in forensic monographs *** authors researched related works and literatures and summarized these with a view to contribute to the existing teaching resources and provide help to forensic practitioners who are involved in scene investigation and identification of such deaths.

关键词： Asphyxia forensic pathology forensic medicine

A Case of Two Full Sisters Share Identical Genotypes on the X Chromosome

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Journal of forensic Science and Medicine 2024年第3期10卷 255-260页

作者： Jinpei Zhang Guangbin Zhao Shicheng Hao Chong Chen Yan Shi Xinyue Xiao Le Wang Li Yuan Collaborative Innovation Center of Judicial Civilization BeijingChina key laboratory of Evidence Science(China University of Political Science and Law) Ministry of EducationBeijingChina key laboratory of forensic genetics of ministry of public security Institute of Forensic ScienceMinistry of Public SecurityBeijingChina Beijing Tongda Shoucheng Institute of forensic Science BeijingChina

X-chromosomal genetic markers are frequently employed in forensic parentage determination owing to their distinctive inheritance *** kinship analysis revealed that two sisters who were not identical twins had identical genotypes on the X chromosome,encompassing 36 X-chromosomal short tandem repeats(X-STRs)and 29 X-chromosomal single-nucleotide polymorphisms(X-SNPs)that spanned the whole X chromosome from the p-telomere to the *** identical X-STRs and X-SNPs in the daughters could be the result of linkage or a rare chance of *** highlights the need for careful analysis and interpretation when dealing with X chromosome markers and that in individual cases,even if two women share an allele at each locus,this does not necessarily mean that they are paternal *** likelihood of random concordance due to maternal alleles must be taken into account.

关键词： Linkage disequilibrium recombination short tandem repeats single-nucleotide polymorphisms X chromosome

Perspectives on the Construction of forensic Autopsy Laboratories Designed to Handle Infectious Diseases

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Journal of forensic Science and Medicine 2020年第2期6卷 62-64页

作者： Jian Zhao Yong Niu Department of forensic Pathology Guangzhou Forensic Science Institute and Key Laboratory of Forensic PathologyMinistry of Public SecurityGuangzhouChina Department of forensic Science Criminal Investigation DepartmentMinistry of Public SecurityBeijingChina

Coronavirus disease 2019 has already infected tens of thousands of people in *** existing autopsy laboratories are not qualified to perform autopsies on individuals confirmed or suspected to have died from an infectious *** dealing with corpses with uncertain health conditions or unknown contact with a pathogen,the forensic medical practitioners face severe risks of being *** meet the requirements of performing autopsies on such bodies,ensure the health and safety of forensic medical practitioners,and study the pathophysiological changes of infected patients,it is necessary to improve the construction requirements and regulations for forensic autopsy laboratories.

关键词： Autopsy laboratory COVID-19 forensic pathology infectious diseases

Compressive Mechanical Properties of Normalized 10^#Low Carbon Steel

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Journal of Beijing Institute of Technology 2017年第4期26卷 434-439页

作者： Jifeng Wei Xin Xie Ziyan Jin Wulong Fan Jifeng Zhang State key laboratory of Explosion Science and Technology Beijing Institute of TechnologyBeijing 100081China key laboratory of forensic Marks Ministry of Public SecurityBeijing 100038China

Compressive mechanical properties of 10^# lowcarbon steel with normalizing heat treatment are studied. A Gleeble system is adopted to analyze the quasi-static properties and thermal softening effects of heat treated 10^# steel,while a Hopkinson bar apparatus is used to investigate its dynamic characteristics under different strain rates. The results showthat yield stress of heat treated 10^# steel is more than that of untreated one at room temperature. When the specimens are tested at different temperatures,yield stresses decrease with increasing temperature except 573 K. Moreover,the influence of strain rate on yield stress are verified,which shows that the yield stress increases sharply from 500 s^-1 to 1 890 s^-1,while it changes a little from 1 890 s^-1 to 4 850 s^-1. The results indicate that yield stress is mainly influenced by hardening effect at lowstrain rate and controlled by both thermal softening effect and strain rate hardening effect at high strain rate.

关键词： mechanical properties Hopkinson bar thermal softening effect strain rate hardening effect