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The soybean PLATZ transcription factor GmPLATZ17 suppresses drought tolerance by interfering with stress-associated gene regulation of GmDREB5

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The crop Journal 2022年第4期10卷 1014-1025页

作者： Juanying Zhao Lei Zheng Jitong Wei Yixuan Wang Jun Chen Yongbin Zhou Ming Chen Fengzhi Wang Youzhi Ma Zhao-Shi Xu Institute of crop Sciences Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic ImprovementKey Laboratory of Biology and Genetic Improvement of Triticeae CropsMinistry of AgricultureBeijing 100081China Hebei key laboratory of crop Salt-alkali Stress Tolerance Evaluation and genetic improvement/Cangzhou Academy of Agriculture and Forestry Sciences Cangzhou 061000HebeiChina

Plant AT-rich sequence and zinc binding(PLATZ)transcription factors are a class of plant specific zincdependent DNA-binding proteins that function in abiotic stress response and plant *** this study,31 GmPLATZ genes were identified in ***17 was down-regulated by drought and exogenous abscisic *** Arabidopsis and soybean hairy roots overexpressing GmPLATZ17 showed drought sensitivity and inhibition of stress-associated gene *** contrast,suppressed expression of GmPLATZ17 led to increased drought tolerance in transgenic soybean hairy *** GmPLATZ17 protein was verified to interact physically with the GmDREB5 transcription factor,and overexpression of GmDREB5 increased drought tolerance in soybean hairy *** of GmPLATZ17 with GmDREB5 was shown to interfere with the DRE-binding activity of GmDREB5,suppressing downstream stress-associated gene *** results show that GmPLATZ17 inhibits drought tolerance by interacting with *** study sheds light on PLATZ transcription factors and the function of GmPLATZ17 in regulating drought sensitivity.

关键词： Soybean PLATZ transcription factor Protein interaction Regulation mechanism Drought response

genetic dissection of carotenoids in maize kernels using high-density single nucleotide polymorphism markers in a recombinant inbred line population

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The crop Journal 2017年第1期5卷 63-72页

作者： Orawan Jittham Xiuyi Fu Jing Xu Subhash Chander Jiansheng Li Xiaohong Yang National Maize improvement Center of China/Beijing key laboratory of crop genetic improvement/China Agricultural University Beijing 100193China

Carotenoids are antioxidants and vitamin A precursors that have important roles in human health. Hence, improving the carotenoid contents in maize kernels is a priority objective for breeders in order to obtain nutritional biofortification outcomes. In the current study, the genetic architecture of carotenoids in maize kernels was explored using a recombinant inbred line(RIL) population derived from a cross between inbred lines By804 and B73. A total of 81 QTLs were detected by using a high-density bin map and a simple sequence repeat(SSR)-based linkage map, with one to seven QTLs for each trait explaining 4.21%–47.53% of the phenotypic variation. A comparison of the QTL mapping efficiency between the two linkage maps revealed that the high-density bin map had higher resolution. In the current study 46 additional QTLs were identified, with 16 being common with previous studies and14 newly identified. Among the results, 29.6%(24/81) of QTLs explained > 10% of the phenotypic variation in the RIL population, and 70.4%(57/81) explained ≤ 10%. These results suggest that a few large-effect QTLs, together with a variable number of minor-effect QTLs,contributed to most of the genetic components of carotenoids in maize kernels.

关键词： Carotenoids QTL mapping SNP SSR Zea mays

Fine Mapping and Cloning of the Grain Number Per-Panicle Gene (Gnp4) on Chromosome 4 in Rice (Oryza sativa L.)

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Agricultural Sciences in China 2011年第12期10卷 1825-1833页

作者： ZHANG Zhan-ying LI Jin-jie YAO Guo-xin ZHANG Hong-liang DOU Hui-jing SHI Hong-li SUN Xing-ming LI Zi-chao key laboratory of crop Heterosis and Utilization Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement ChinaAgricultural University Beijing 100193 P.R.China

Grain number per-panicle is one of the most important components for rice yield. Spikelets on the primary and secondary branches determine the grain number per-panicle in rice. In this study, we identified a natural mutant, gnp4, lack of lateral spikelet on the secondary branches in the field condition. In addition, the Gnp4 and Lax1-1 double mutant showed dramatically reduced secondary branches and spikelets in panicle at reproductive stage, and tillers at vegetative stage. By map-based cloning approach, and using four F2 segregating populations, the Gnp4 gene was finally mapped to a 10.7-kb region on the long arm of chromosome 4 in rice. In this region, only one gene was predicted, and genomic DNA sequencing of the 10.7-kb region showed no nucleotide differences between the mutant and wild type. Interestingly, we found that the methylation level of several cytosines in the promoter CpG islands region of the predicted gene in gnp4 were different from the wild type. Thus, we propose that the DNA methylation changes at these sites may induce to decrease expression level of Gnp4, consequently, resulting in phenotypic variation.

关键词： rice gnp4 laxl-1 grain number per-panicle methylation

Fine Mapping of RppP25, a Southern Rust Resistance Gene in Maize

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Journal of Integrative Plant Biology 2013年第5期55卷 462-472页

作者： Panfeng Zhao Guobin Zhang Xiaojun Wu Na Li Dianyi Shi Dengfeng Zhang Chunfang Ji Mingliang Xu Shoucai Wang National Maize improvement Center of China Key Laboratory of Biology and Genetic Improvement of Maize (Ministry of Agriculture) China Agricultural University National key Facility for crop Gene Resources and genetic improvement (NFCRI) Institute of Crop ScienceChinese Academy of Agricultural Sciences

Southern rust （Puccinia polysora Underw.） is a major disease that can cause severe yield losses in maize （Zea mays L.）. In our previous study, a major gene RppP25 that confers resistance to southern rust was identified in inbred line P25. Here, we report the fine mapping and candidate gene analysis of RppP25 from the near-isogenic line F939, which harbors RppP25 in the genetic background of the susceptible inbred line F349. The inheritance of resistance to southern rust was investigated in the BC1F1 and BC3F1 populations, which were derived from a cross between F939 and F349 （as the recurrent parent）. The 1：1 segregation ratio of resistance to susceptible plants in these two populations indicated that the resistance is controlled by a single dominant gene. Ten markers, including three simple sequence repeat （SSR） markers and seven insertion/deletion （InDel） markers, were developed in the RppP25 region. RppP25 was delimited to an interval between P091 and M271, with an estimated length of 40 kb based on the physical map of B73. In this region, a candidate gene was identified that was predicted to encode a putative nucleotide-binding site leucine-rich repeat （NBS-LRR） protein. Two co-segregated markers will aid in pyramiding diverse southern rust resistance alleles into elite materials, and thereby improve southern rust resistance worldwide.

关键词： Fine mapping maize Puccinia polysora resistance gene southern rust.

Molecular Cloning,Expression Analysis and Localization of Exo70A1 Related to Self Incompatibility in Non-Heading Chinese Cabbage(Brassica campestris ***)

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Journal of Integrative Agriculture 2013年第12期12卷 2149-2156页

作者： WANG Li GE Ting-ting PENG Hai-tao WANG Cheng LIU Tong-kun HOU Xi-lin LI Ying Horticultural Department Nanjing Agricultural University State key laboratory of crop genetics and Germplasm Enhancement key laboratory of Southern Vegetable crop genetic improvement Ministry of Agriculture

The exocyst is a conserved protein complex,and required for vesicles tethering,fusion and polarized ***70A1,the exocyst subunit,is essential for assembly of the exocyst *** better understand potential roles of Exo70A1 in non-heading Chinese cabbage（Brassica campestris ***）,we obtained the full-length cDNA of Exo70A1 gene,which consisted of 1 917 bp and encoded a protein of 638 amino *** showed BcExo70A1 shared 94.9% identity with Brassica oleracea ***（AEI26267.1）,and clustered into a same group with other homologues in *** *** and Brassica *** localization analysis showed BcExo70A1 was localized to punctate structures in cytosol of onion epithelial *** showed that BcExo70A1 was widely presented in stamens,young stems,petals,unpollinated pistils,roots and leaves of self compatible and incompatible *** transcripts of BcExo70A1 in non- heading Chinese cabbage declined during initial 1.5 h after incompatible pollination,while an opposite trend was presented after compatible *** study reveals that BcExo70A1 could play essential roles in plant growth and development,and is related to the rejection of self pollen in non-heading Chinese cabbage.

关键词： Exo70A1 gene expression subcellular localization self-incompatibility non-heading Chinese cabbage

Isolation and Characterization of Copia-like Retrotransposons from 12 Sweet Orange （Citrus sinensis Osbeck） Cultivars

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Journal of Integrative Plant Biology 2005年第12期47卷 1507-1515页

作者： Neng-Guo TAO Juan XU Yun-Jiang CHENG Liu HONG Wen-Wu GUO Hua-Lin YI Xiu-Xin DENG State key laboratory of crop genetic improvement National Center of Crop Molecular Breeding Huazhong Agricultural University Wuhan 430070 China

As the largest transposable element in the plant genome, retrotransposons are thought to be involved in citrus genetic instability and genome evolution, especially in sweet orange, which is prone to bud mutation. In the present study, the presence of copia-like retrotransposons, their heterogeneity, genomic distribution, and transcriptional activities in Citrus were investigated in 12 sweet orange （Citrus sinensis Osbeck） cultivars using a PCR assay designed to detect copia-like reverse transcriptase （RT） sequences. Twelve amplification products from each cultivar were cloned and sequenced. The cloned sequences showed great heterogeneity, except “Dream” navel and “Hamlin”, both of which shared the same sequence. Frame shifting, termination, deletion, and substitution accounted for the heterogeneity of RT sequences. Southern blot hybridization using the RT1 clone from the “Cara Cara” navel as a probe showed that multiple copies were integrated throughout the sweet orange genomes, which made the retrotransposon possible an effective molecular marker to detect citrus evolution events and to reveal its relationship with bud mutation. No transcriptional activities of the retrotransposon were detected by RT-PCR and Northern analysis in the fruits and leaves of either “Cara Cara” or “Seike” navels.

关键词： bud mutation Citrus cloning PCR retrotransposons.

Identification of QTLs for Starch Content in Sweetpotato(Ipomoea batatas(L.) Lam.)

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Journal of Integrative Agriculture 2014年第2期13卷 310-315页

作者： YU Xiao-xia ZHAO Ning LI Hui JIE Qin ZHAI Hong HE Shao-zhen LI Qiang LIU Qing-chang laboratory of crop Heterosis and Utilization Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement/China Agricultural University Xuzhou Sweetpotato Research Center

Sweetpotato （Ipomoea batatas （L.） Lam.） breeding is challenging due to its genetic complexity. In the present study, interval mapping （IM） and multiple quantitative trait locus （QTL） model （MQM） analysis were used to identify QTLs for starch content with a mapping population consisting of 202 F1 individuals of a cross between Xushu 18, a cultivar susceptible to stem nematodes, with high yield and moderate starch, and Xu 781, which is resistant to stem nematodes, has low yield and high starch content. Six QTLs for starch content were mapped on six linkage groups of the Xu 781 map, explaining 9.1-38.8% of the variation. Especially, one of them, DMFN 4, accounted for 38.8% of starch content variation, which is the QTL that explains the highest phenotypic variation detected to date in sweetpotato. All of the six QTLs had a positive effect on the variation of the starch content, which indicated the inheritance derived from the parent Xu 781. Two QTLs for starch content were detected on two linkage groups of the Xushu 18 map, explaining 14.3 and 16.1% of the variation, respectively. They had a negative effect on the variation, indicating the inheritance derived from Xu 781. Seven of eight QTLs were co-localized with a single marker. This is the first report on the development of QTLs co-localized with a single marker in sweetpotato. These QTLs and their co-localized markers may be used in marker-assisted breeding for the starch content of sweetpotato.

关键词： co-localized marker lpomoea batatas (L.) Lam. QTLs starch content

Development of SRAP Markers Linked to a Gene for Stem Nematode Resistance in Sweetpotato, Ipomoea batatas (L.) Lam.

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Journal of Integrative Agriculture 2013年第3期12卷 414-419页

作者： ZHAO Ning ZHAI Hong YU Xiao-xia LIU Zhe-sheng HE Shao-zhen LI Qiang MA Dai-fu LIU Qing-chang Beijing key laboratory of crop genetic improvement/laboratory of crop Heterosis and Utilization Ministry of Education/China Agricultural University Xuzhou Sweetpotato Research Center

Sequence-related amplification polymorphism （SRAP） markers closely linked to stem nematode resistance gene were developed in sweetpotato, lpomoea batatas （L.） Lam. Using bulked segregant analysis （BSA）, 200 SRAP primer combinations were screened with the resistant and susceptible bulked DNA from the 196 progenies of an F1 single-cross population of resistant parent Xu 781xsusceptible parent Xushu 18, 77 of them showed polymorphic bands between resistant and susceptible DNA. Primer combinations detecting polymorphism between the two bulks were used to screen both parents and 10 individuals from each of the bulks. The results showed that primer combination A9B4 produced 3 specific bands in the resistant plants but not in the susceptible plants, suggesting that the markers, named Nspl, Nsp2 and Nsp3, respectively, linked to a gene for stem nematode resistance. Primer combination A3B6 also produced a SRAP marker named Nsp4 linking to the resistance gene. Amplified analysis of the 196 F1 individuals indicated that the genetic distance between these markers and the resistance gene was 4.7, 4.7, 6.3, and 9.6 cM, respectively.

关键词： Ipomoea batatas (L.) Lam. SRAP marker stem nematode

RNA editing machinery in plant organelles

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Science China(Life Sciences) 2018年第2期61卷 162-169页

作者： Junjie Yan Qunxia Zhang Ping Yin National key laboratory of crop genetic improvement and National Centre of Plant Gene Research Huazhong Agricultural University

RNA editing is a type of post-transcriptional modification that includes nucleotide insertion/deletion or conversion. Different categories of RNA editing have been widely observed in distinct RNAs from divergent organisms. In flowering plants, RNA editing usually alters cytidine to uridine in plastids and mitochondria, playing important roles in various plant developmental processes, including organelle biogenesis, adaptation to environmental changes, and signal transduction. Numerous studies have demonstrated that a number of factors are involved in plant RNA editing, such as pentatricopeptide repeat(PPR) proteins, multiple organelle RNA editing factors(MORF, also known as RIP), organelle RNA recognition motif(ORRM) containing proteins,protoporphyrinogen IX oxidase 1(PPO1) and organelle zinc finger 1(OZ1). These factors play diverse roles in plant RNA editing due to their distinct characteristics. In this review, we discuss the functional roles of the individual editing factors and their associations in plant RNA editing.

关键词： RNA editing editosome plant organelles