The soybean PLATZ transcription factor GmPLATZ17 suppresses drought tolerance by interfering with stress-associated gene regulation of GmDREB5

作     者：Juanying Zhao Lei Zheng Jitong Wei Yixuan Wang Jun Chen Yongbin Zhou Ming Chen Fengzhi Wang Youzhi Ma Zhao-Shi Xu Juanying Zhao;Lei Zheng;Jitong Wei;Yixuan Wang;Jun Chen;Yongbin Zhou;Ming Chen;Fengzhi Wang;Youzhi Ma;Zhao-Shi Xu

作者机构：Institute of Crop SciencesChinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic ImprovementKey Laboratory of Biology and Genetic Improvement of Triticeae CropsMinistry of AgricultureBeijing 100081China Hebei Key Laboratory of Crop Salt-alkali Stress Tolerance Evaluation and Genetic Improvement/Cangzhou Academy of Agriculture and Forestry SciencesCangzhou 061000HebeiChina 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2022年第10卷第4期

页      面：1014-1025页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China(31871624) the Agricultural Science and Technology Innovation Program(CAAS-ZDRW202109 and CAAS-ZDRW202002) the Central Public-interest Scientific Institution Basal Research Fund. 

主　　题：Soybean PLATZ transcription factor Protein interaction Regulation mechanism Drought response 

摘      要：Plant AT-rich sequence and zinc binding(PLATZ)transcription factors are a class of plant specific zincdependent DNA-binding proteins that function in abiotic stress response and plant development.In this study,31 GmPLATZ genes were identified in soybean.GmPLATZ17 was down-regulated by drought and exogenous abscisic acid.Transgenic Arabidopsis and soybean hairy roots overexpressing GmPLATZ17 showed drought sensitivity and inhibition of stress-associated gene transcription.In contrast,suppressed expression of GmPLATZ17 led to increased drought tolerance in transgenic soybean hairy roots.The GmPLATZ17 protein was verified to interact physically with the GmDREB5 transcription factor,and overexpression of GmDREB5 increased drought tolerance in soybean hairy roots.Interaction of GmPLATZ17 with GmDREB5 was shown to interfere with the DRE-binding activity of GmDREB5,suppressing downstream stress-associated gene expression.These results show that GmPLATZ17 inhibits drought tolerance by interacting with GmDREB5.This study sheds light on PLATZ transcription factors and the function of GmPLATZ17 in regulating drought sensitivity.